LOCUS       NM_007382               2062 bp    mRNA    linear   ROD 04-DEC-2024
DEFINITION  Mus musculus acyl-Coenzyme A dehydrogenase, medium chain (Acadm),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_007382
VERSION     NM_007382.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Karunanidhi,A., Basu,S., Zhao,X.J., D'Annibale,O., Van't Land,C.,
            Vockley,J. and Mohsen,A.W.
  TITLE     Heptanoic and medium branched-chain fatty acids as anaplerotic
            treatment for medium chain acyl-CoA dehydrogenase deficiency
  JOURNAL   Mol Genet Metab 140 (3), 107689 (2023)
   PUBMED   37660571
REFERENCE   3  (bases 1 to 2062)
  AUTHORS   Pruss,K.M., Chen,H., Liu,Y., Van Treuren,W., Higginbottom,S.K.,
            Jarman,J.B., Fischer,C.R., Mak,J., Wong,B., Cowan,T.M.,
            Fischbach,M.A., Sonnenburg,J.L. and Dodd,D.
  TITLE     Host-microbe co-metabolism via MCAD generates circulating
            metabolites including hippuric acid
  JOURNAL   Nat Commun 14 (1), 512 (2023)
   PUBMED   36720857
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2062)
  AUTHORS   Zou,J., Wang,W., Lu,Y., Ayala,J., Dong,K., Zhou,H., Wang,J.,
            Chen,W., Weintraub,N.L., Zhou,J., Li,J. and Su,H.
  TITLE     Neddylation is required for perinatal cardiac development through
            stimulation of metabolic maturation
  JOURNAL   Cell Rep 42 (1), 112018 (2023)
   PUBMED   36662623
REFERENCE   5  (bases 1 to 2062)
  AUTHORS   Kurtz,D.M., Rinaldo,P., Rhead,W.J., Tian,L., Millington,D.S.,
            Vockley,J., Hamm,D.A., Brix,A.E., Lindsey,J.R., Pinkert,C.A.,
            O'Brien,W.E. and Wood,P.A.
  TITLE     Targeted disruption of mouse long-chain acyl-CoA dehydrogenase gene
            reveals crucial roles for fatty acid oxidation
  JOURNAL   Proc Natl Acad Sci U S A 95 (26), 15592-15597 (1998)
   PUBMED   9861014
REFERENCE   6  (bases 1 to 2062)
  AUTHORS   Ruiz-Lozano,P., Smith,S.M., Perkins,G., Kubalak,S.W., Boss,G.R.,
            Sucov,H.M., Evans,R.M. and Chien,K.R.
  TITLE     Energy deprivation and a deficiency in downstream metabolic target
            genes during the onset of embryonic heart failure in RXRalpha-/-
            embryos
  JOURNAL   Development 125 (3), 533-544 (1998)
   PUBMED   9425147
REFERENCE   7  (bases 1 to 2062)
  AUTHORS   Sladek,R., Bader,J.A. and Giguere,V.
  TITLE     The orphan nuclear receptor estrogen-related receptor alpha is a
            transcriptional regulator of the human medium-chain acyl coenzyme A
            dehydrogenase gene
  JOURNAL   Mol Cell Biol 17 (9), 5400-5409 (1997)
   PUBMED   9271417
REFERENCE   8  (bases 1 to 2062)
  AUTHORS   Tolwani,R.J., Farmer,S.C., Johnson,K.R., Davisson,M.T., Kurtz,D.M.,
            Hinsdale,M.E., Cresci,S., Kelly,D.P. and Wood,P.A.
  TITLE     Structure and chromosomal location of the mouse medium-chain
            acyl-CoA dehydrogenase-encoding gene and its promoter
  JOURNAL   Gene 170 (2), 165-171 (1996)
   PUBMED   8666240
REFERENCE   9  (bases 1 to 2062)
  AUTHORS   Tolwani,R.J., Farmer,S.C. and Wood,P.A.
  TITLE     Molecular cloning and characterization of the mouse medium-chain
            acyl-CoA dehydrogenase cDNA
  JOURNAL   Genomics 23 (1), 247-249 (1994)
   PUBMED   7829081
REFERENCE   10 (bases 1 to 2062)
  AUTHORS   Wood,P.A., Farmer,S.C., Tolwani,R.J., Warren,J.R., Steinkampf,M.P.,
            Johnson,L.W., Mountz,J.D. and Kelly,D.P.
  TITLE     Molecular studies of mouse medium and long-chain acyl-CoA
            dehydrogenase genes for site-directed mutagenesis of embryonic stem
            cells
  JOURNAL   Prog Clin Biol Res 375, 151-160 (1992)
   PUBMED   1438358
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC087184.7, CX232934.1,
            AK146444.1 and BB745012.1.
            
            On Nov 28, 2012 this sequence version replaced NM_007382.4.
            
            Summary: This gene encodes a homotetrameric mitochondrial
            flavoprotein and is a member of the acyl-CoA dehydrogenase family.
            Members of this family catalyze the first step of fatty acid
            beta-oxidation, forming a C2-C3 trans-double bond in a
            FAD-dependent reaction. As beta-oxidation cycles through its four
            steps, each member of the acyl-CoA dehydrogenase family works at an
            optimum fatty acid chain-length. This enzyme has its optimum length
            between C6- and C12-acylCoA. In mice, deficiency of this gene can
            cause neonatal mortality as well as fasting and cold intolerance.
            This gene has multiple, intronless pseudogenes. [provided by
            RefSeq, Nov 2012].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments. The transit
            peptide annotated at aa 1-25 is inferred by similarity to the
            cleavage site in the orthologous rat protein as described in PMID:
            2777793. The software program TargetP1.1 predicts that the transit
            peptide in mouse is aa 1-27.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC013498.1, AK146444.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849390, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 AC087184.7         130830-130838       c
            10-433              CX232934.1         1-424
            434-1965            AK146444.1         239-1770
            1966-2062           BB745012.1         367-463
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 78.77 cM"
     gene            1..2062
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /note="acyl-Coenzyme A dehydrogenase, medium chain"
                     /db_xref="GeneID:11364"
                     /db_xref="MGI:MGI:87867"
     exon            1..248
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            249..336
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            337..434
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            435..504
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            505..605
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            606..686
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            687..817
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            818..926
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1067
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1163
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1412
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..2062
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2038..2043
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /note="hexamer: AATAAA"
     polyA_site      2058
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /note="major polyA site"
ORIGIN      
        1 ccgaagtcag tctgagagct cagacctcat ctgtgtgtaa agggactccc taggccacgc
       61 ccccgaggtc tgcagacttt gacccgcacg cctccgcacc cctagagtct aaggcccgcc
      121 cctctcctcc ctccaagcca cgcctcctgg gggcgggacc cccgtccgtt ttgcttgctg
      181 cacggaggtg gtcggaggca gcagatcgag gagccatcat ggcagcggcg ttccgcagag
      241 gctgcagggt cctgagaagt gtttctcatt ttgagtgtcg aacacaacac tcgaaagcgg
      301 ctcacaagca ggagcccgga ttagggttta gttttgagtt gacggaacag cagaaagagt
      361 ttcaagcaac tgcccgcaag tttgccagag aggagattat ccccgtcgcc ccggaatatg
      421 acaaaagcgg ggagtacccg ttccctctca tcaaaagagc ctgggaactc ggcttgatca
      481 acgcgcacat tccggaaagt tgcggtggcc ttggcctggg aacgttcgat gcttgtttaa
      541 ttaccgaaga gttggcgtat gggtgtacag gggtgcaaac tgctattgaa gcaaattctt
      601 tggggcaaat gcctgtgatt cttgctggaa atgatcaaca aaaaaagaag tatttgggga
      661 ggatgacgga gcagccaatg atgtgtgctt actgtgtgac agagccctcc gcaggctctg
      721 atgtggcggc cattaagacc aaagcagaga agaagggtga cgagtatgtt atcaacggcc
      781 agaagatgtg gataaccaac gggggaaagg ccaactggta tttcttgttg gcacgttcta
      841 acccagatcc taaagtaccc gctagtaaag cctttactgg attcattgtg gaagccgaca
      901 ccccgggaat acatattgga aaaaaggaac taaacatggg ccagcgatgc tctgacacca
      961 gaggaattgc tttcgaagac gtcagagtgc ctaaggaaaa tgtgttaatc ggtgaaggag
     1021 caggtttcaa gatcgcaatg ggtgcttttg atagaaccag acctacagtc gcagctggcg
     1081 ctgtcgggct agcccagaga gctctagacg aagccacgaa gtatgccctg gataggaaga
     1141 catttggaaa gctgctagtg gagcaccaag gagtttcatt tctgctcgca gaaatggcga
     1201 tgaaggttga actcgctagg ctcagttacc agagagcagc ctgggaggtt gactccggtc
     1261 gccggaacac ttactatgcc tcgattgcaa aggcctttgc tggagacatt gccaatcagc
     1321 tagccactga cgccgtgcag attttcggag gctatggatt caacacagag taccctgtgg
     1381 agaagctgat gagggacgcc aagatctatc agatttatga aggtactgca caaattcaga
     1441 ggctgatcat agctcgtgag cacattgaaa agtataaaaa ttaacaggaa ttactattga
     1501 acgatgcatc accctcgtgt aactaagctc caagcactgt tgctgcttca ggggaaaagg
     1561 gctttactgt cttcccaagg aaatgagatc aaagacgagt ttggatctgt gcagcggatt
     1621 cccatggcgg aggaacctgt cttcagctct atggtgaccc tttctagata ggtttggctt
     1681 ttggacaatg attggtcctt agccccgaat tgtgttagtt tgctctttga tcacttaaaa
     1741 tggaaaaaca ccctggactt ttaatgttca ttcaagtgac aggaaaggcg gcttgtcaag
     1801 gaagaactca tgattctaac ataaacactg aaaatttgtg gtagattgga cacgtcagac
     1861 tgtgacatag cagcatttct gtgctgaact gttaatttta taattttgat tatatttgct
     1921 ttgttttgca caaaagagta aaaagtttat attcatattc tcccattata aaactaaaac
     1981 ttttctggga atcttagtac tgaacgagaa ttttatttgt cttgtttaaa ataattcaat
     2041 aaagctaacc ttaacttatt tt
//
