LOCUS       NM_025862               2865 bp    mRNA    linear   ROD 26-JUN-2024
DEFINITION  Mus musculus acyl-Coenzyme A dehydrogenase family, member 8
            (Acad8), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_025862
VERSION     NM_025862.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Zhang,D., Deng,W., Jiang,T., Zhao,Y., Bai,D., Tian,Y., Kong,S.,
            Zhang,L., Wang,H., Gao,S. and Lu,Z.
  TITLE     Maternal Ezh1/2 deficiency impairs the function of mitochondria in
            mouse oocytes and early embryos
  JOURNAL   J Cell Physiol 239 (6), e31244 (2024)
   PUBMED   38529784
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Peng,M.Z., Shao,Y.X., Li,X.Z., Zhang,K.D., Cai,Y.N., Lin,Y.T.,
            Jiang,M.Y., Liu,Z.C., Su,X.Y., Zhang,W., Jiang,X.L. and Liu,L.
  TITLE     Mitochondrial FAD shortage in SLC25A32 deficiency affects
            folate-mediated one-carbon metabolism
  JOURNAL   Cell Mol Life Sci 79 (7), 375 (2022)
   PUBMED   35727412
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2865)
  AUTHORS   Dani,N., Herbst,R.H., McCabe,C., Green,G.S., Kaiser,K., Head,J.P.,
            Cui,J., Shipley,F.B., Jang,A., Dionne,D., Nguyen,L., Rodman,C.,
            Riesenfeld,S.J., Prochazka,J., Prochazkova,M., Sedlacek,R.,
            Zhang,F., Bryja,V., Rozenblatt-Rosen,O., Habib,N., Regev,A. and
            Lehtinen,M.K.
  TITLE     A cellular and spatial map of the choroid plexus across brain
            ventricles and ages
  JOURNAL   Cell 184 (11), 3056-3074 (2021)
   PUBMED   33932339
REFERENCE   4  (bases 1 to 2865)
  AUTHORS   Russo,L.M., Abdeltawab,N.F., O'Brien,A.D., Kotb,M. and
            Melton-Celsa,A.R.
  TITLE     Mapping of genetic loci that modulate differential colonization by
            Escherichia coli O157:H7 TUV86-2 in advanced recombinant inbred BXD
            mice
  JOURNAL   BMC Genomics 16, 947 (2015)
   PUBMED   26573818
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2865)
  AUTHORS   Sabbagha,N.G., Kao,H.J., Yang,C.F., Huang,C.C., Lin,W.D.,
            Tsai,F.J., Chen,T.H., Tarn,W.Y., Wu,J.Y. and Chen,Y.T.
  TITLE     Alternative splicing in Acad8 resulting a mitochondrial defect and
            progressive hepatic steatosis in mice
  JOURNAL   Pediatr Res 70 (1), 31-36 (2011)
   PUBMED   21659959
  REMARK    GeneRIF: N-ethyl-N-nitrosourea-mediated mutagenesis caused
            alternative splicing in Acad8 resulting a mitochondrial defect and
            progressive hepatic steatosis in mice.
REFERENCE   6  (bases 1 to 2865)
  AUTHORS   Quina,L.A., Wang,S., Ng,L. and Turner,E.E.
  TITLE     Brn3a and Nurr1 mediate a gene regulatory pathway for habenula
            development
  JOURNAL   J Neurosci 29 (45), 14309-14322 (2009)
   PUBMED   19906978
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BQ931973.1, AK077335.1 and AC138616.4.
            
            On Nov 25, 2006 this sequence version replaced NM_025862.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK019502.1, AK029897.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-714               BQ931973.1         1-714
            715-1949            AK077335.1         695-1929
            1950-2865           AC138616.4         182743-183658       c
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 12.0 cM"
     gene            1..2865
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /note="acyl-Coenzyme A dehydrogenase family, member 8"
                     /db_xref="GeneID:66948"
                     /db_xref="MGI:MGI:1914198"
     exon            1..156
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..1295
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /EC_number="1.3.8.5"
                     /note="isobutyryl-CoA dehydrogenase, mitochondrial;
                     ACAD-8; acyl-CoA dehydrogenase family member 8"
                     /codon_start=1
                     /product="isobutyryl-CoA dehydrogenase, mitochondrial
                     precursor"
                     /protein_id="NP_080138.2"
                     /db_xref="CCDS:CCDS40567.1"
                     /db_xref="GeneID:66948"
                     /db_xref="MGI:MGI:1914198"
                     /translation="MAMLRSGYRRFGCLRAALKSLAQTHHRSITFCIDPSLGLNEEQK
                     GFQKVAFDFAAREMAPNMAEWDQKELFPVDVMRKAAQLGFGGVYVRTDVGGSGLSRLD
                     TSVIFEALATGCTSTTAYISIHNMCAWMIDSFGNEEQRHKFCPPLCTMEKFASYCLTE
                     PGSGSDAASLLTSAKQQGDHYILNGSKAFISGGGESDIYVVMCRTGESGAKGISCIVV
                     EKGTPGLSFGKKEKKVGWNSQPTRAVIFEDCAVPVANRIGTEGQGFLIAMKGLNGGRI
                     NVASCSLGAAHASVILTQEHLKVRKQFGAPLARSQYLQFQLADMATKLVASRLMIRTA
                     AVALQEEREDAVALCSMAKLFATEECFAICNQALQMHGGYGYLKDYAVQQYMRDSRVH
                     QILEGSNEVMRMLISRNLLQD"
     exon            157..257
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            258..427
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            428..537
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            538..614
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            615..752
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            753..888
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            889..986
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1139
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1242
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..2865
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1818..1823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1849
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /note="major polyA site"
     regulatory      2004..2009
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2035
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
     regulatory      2830..2835
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /note="hexamer: AGTAAA"
     regulatory      2838..2843
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      2849
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
     polyA_site      2865
                     /gene="Acad8"
                     /gene_synonym="2310016C19Rik"
ORIGIN      
        1 cggctagttc tggcctgggc taagaggcct ggggtctgag attcgaagtt tacatggcga
       61 tgttgcggtc cggatacagg cgctttggct gtctgcgcgc ggcactcaag tccctggctc
      121 agacccacca ccggagcatc accttctgca tcgatccttc cttggggcta aatgaagaac
      181 agaaaggatt tcagaaagtg gcctttgact ttgcagctcg ggaaatggct cccaatatgg
      241 cggagtggga tcagaaggag ctgttccctg tggatgtgat gcggaaggct gcacagctgg
      301 gatttggggg ggtctatgtg cgaacagatg tgggtggatc tggactgtct cgccttgata
      361 cttctgtcat ctttgaagcc ttggccacag gctgcaccag caccactgcc tatataagca
      421 tccacaacat gtgtgcctgg atgattgata gctttggaaa tgaggaacag aggcacaaat
      481 tttgcccacc gctctgtacc atggagaagt ttgcttccta ctgcctcact gaaccaggca
      541 gtgggagtga tgctgcatct cttctgacct cagctaaaca acaaggagat cattacatcc
      601 tcaatggttc caaggccttc atcagtgggg gaggtgagtc agacatctat gtggtcatgt
      661 gccgaactgg tgagtctggc gccaaaggta tctcctgcat agttgttgag aaaggaaccc
      721 ctggtctcag ctttggcaag aaggagaaga aggtggggtg gaactcgcag ccaacccgag
      781 cagtgatctt tgaagactgt gctgtccctg tggccaacag gattgggacc gaggggcagg
      841 gttttctcat cgccatgaaa ggactgaatg gtggaaggat caatgttgca tcctgctctc
      901 tgggagccgc tcatgcttca gttatcctaa cccaagaaca cctcaaggtc cgcaagcagt
      961 ttggagcacc tttggccaga agccagtacc tccaattcca gttggcagat atggccacca
     1021 agctagttgc ttcccggctg atgatccgta ctgcagcagt ggctttgcag gaagagcggg
     1081 aagatgcagt ggccctgtgt tccatggcca agctctttgc tacagaggag tgctttgcta
     1141 tctgcaacca ggctttgcag atgcatgggg gctacggcta tctgaaggac tatgctgttc
     1201 aacagtatat gcgggactcc agggtccacc agatcctaga aggtagcaat gaagtgatga
     1261 ggatgctgat ctctcgaaac ctgcttcagg actagctccc aagctggaga gcctcgcctg
     1321 gagtctgtgt gcagctggaa tcagtcttga tgctcctatg ggctgctcag ttcaagtgaa
     1381 tcatgaagta gaccaaaggg ctgagctctt ctaaacagac tgagctgtca gccacgctgc
     1441 tggttctgag acaagaacag agttcttagc agagctagaa gggctgctct gaggagccat
     1501 gcccaaagaa gacctgcaca ctgccttcct cattctggaa agacaagcgg tcaaagctgt
     1561 ggcttggatc cacactatgg gcattttggt gattcttgtc cttagggtct gggtacttga
     1621 atgaagcttt tcctgattaa acaggtaaag tctggggagg ccagggcagt ggacagatct
     1681 gcccctttct ggtttctgtt ctgcactagg ggtatgtcag gacagtactc ctgtactcag
     1741 atcatgatga aataaatatt tgggaaccta ctgctaagca atgagttagg gtgcaccttt
     1801 aactccagga accatgaaat aaatgtcaag ggctaagtct gttttggaat atttggattt
     1861 tttatttggg atttatttcc tctggggccc ccttctcttt ttaagtatat ttaattagtc
     1921 ccttgataag caaaatctat gtctttactg cctccttcaa ctataagact taaaactgta
     1981 tttgagattt tatattatat tcgattaaat atttaattgg attttagaac aattagaaat
     2041 ttctcctaag gcagtttgtc ttttaaaaag tacattcttt taatgatttt gcaaacaaag
     2101 cattatttgt catgtgtgaa tgtctaagta ttaggtatag tctcctgcag tggcatctta
     2161 cagctcgtca tggagtcagc ccggctcagt cttctcaaga tgtccctcac agcccccaga
     2221 caaaaaccaa aaacccagac caacaaaaca tgacgtttgt gacattctga cttagtttct
     2281 tggtgacacc actcaacttg tggtattttg ttacggtatt ctgagctgag taatataatc
     2341 aacctcactg atgtcttata aagaagtgtg accaaatgct ttctccaaaa gcaaaatagg
     2401 gctgaggcta tggatcagtg gtacagtgtt tgccttgtgt gtttgttcca cagcatcaaa
     2461 aataagttaa taaatcacag tgtattgttt cccaatacta acaataagaa atttaaaata
     2521 tcacagtagt ctccagttta catttcagta taaatataaa gttatacaga gcctctctac
     2581 tgaacataat taacactgtt acaaaaattt taaagttata gacagaatac cgatattaac
     2641 attaaagttt gccttttagt taatttcagc taaaatcatt gcagttaaaa attaaatttt
     2701 gtgtgtggca ggggaggtga ttgttgaaaa tagaatatga caaacttatt ctaaaagtat
     2761 ggaaatacaa aggatatgtc cagtgttgaa tatttactat tgaatattca atttgatttt
     2821 gaatatataa gtaaaagagt aaagttgaag atcattttga aaaaa
//
