LOCUS       NM_134076               2351 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus abhydrolase domain containing 4 (Abhd4), transcript
            variant 1, mRNA.
ACCESSION   NM_134076
VERSION     NM_134076.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Seramur,M.E., Sink,S., Cox,A.O., Furdui,C.M. and Key,C.C.
  TITLE     ABHD4 regulates adipocyte differentiation in vitro but does not
            affect adipose tissue lipid metabolism in mice
  JOURNAL   J Lipid Res 64 (8), 100405 (2023)
   PUBMED   37352974
  REMARK    GeneRIF: ABHD4 regulates adipocyte differentiation in vitro but
            does not affect adipose tissue lipid metabolism in mice.
REFERENCE   3  (bases 1 to 2351)
  AUTHORS   Laszlo,Z.I., Lele,Z., Zoldi,M., Miczan,V., Mogor,F., Simon,G.M.,
            Mackie,K., Kacskovics,I., Cravatt,B.F. and Katona,I.
  TITLE     ABHD4-dependent developmental anoikis safeguards the embryonic
            brain
  JOURNAL   Nat Commun 11 (1), 4363 (2020)
   PUBMED   32868797
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2351)
  AUTHORS   Lee,H.C., Simon,G.M. and Cravatt,B.F.
  TITLE     ABHD4 regulates multiple classes of N-acyl phospholipids in the
            mammalian central nervous system
  JOURNAL   Biochemistry 54 (15), 2539-2549 (2015)
   PUBMED   25853435
  REMARK    GeneRIF: These findings, taken together, designate ABHD4 as a
            principal regulator of N-acyl phospholipid metabolism in the
            mammalian nervous system.
REFERENCE   5  (bases 1 to 2351)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   6  (bases 1 to 2351)
  AUTHORS   Simon,G.M. and Cravatt,B.F.
  TITLE     Endocannabinoid biosynthesis proceeding through
            glycerophospho-N-acyl ethanolamine and a role for
            alpha/beta-hydrolase 4 in this pathway
  JOURNAL   J Biol Chem 281 (36), 26465-26472 (2006)
   PUBMED   16818490
  REMARK    GeneRIF: Lysophospholipase/phospholipase B (EC 3.1.1.5) specific
            for N-acyl phosphatidylethanolamine (NAPE) suggesting a role in the
            biosynthesis of N-acyl ethanolamines including the endocannabinoid
            anandamide.
REFERENCE   7  (bases 1 to 2351)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT573018.19.
            
            On May 17, 2019 this sequence version replaced NM_134076.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC017532.1, AK161853.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                CT573018.19        90666-90702         c
            38-126              CT573018.19        88271-88359         c
            127-499             CT573018.19        86962-87334         c
            500-654             CT573018.19        86662-86816         c
            655-766             CT573018.19        84593-84704         c
            767-953             CT573018.19        82804-82990         c
            954-2351            CT573018.19        80820-82217         c
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 27.71 cM"
     gene            1..2351
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /note="abhydrolase domain containing 4"
                     /db_xref="GeneID:105501"
                     /db_xref="MGI:MGI:1915938"
     exon            1..37
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1043
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     abhydrolase domain-containing protein 4;
                     alpha/beta-hydrolase 4;
                     lyso-N-acylphosphatidylethanolamine lipase; alpha/beta
                     hydrolase domain-containing protein 4; protein ABHD4;
                     (Lyso)-N-acylphosphatidylethanolamine lipase"
                     /codon_start=1
                     /product="(Lyso)-N-acylphosphatidylethanolamine lipase
                     isoform 1"
                     /protein_id="NP_598837.3"
                     /db_xref="CCDS:CCDS56954.1"
                     /db_xref="GeneID:105501"
                     /db_xref="MGI:MGI:1915938"
                     /translation="MADDLEQQPQGWLSSWLPTWRPTSMSQLKNVEARILQCLQNKFL
                     ARYVSLPNQNKIWTVTVSPEQKDRTPLVMVHGFGGGVGLWILNMDSLSARRTLHTFDL
                     LGFGRSSRPTFPRDPEGAEDEFVASIETWRETMGIPTMILLGHSLGGFLATSYSIKYP
                     ERVKHLILVDPWGFPLRPTDPSEIRAPPTWVKAVASVLGRSNPLAVLRVAGPWGPGLV
                     QRFRPDFKRKFADFFEDDTISEYIYHCNAQNPSGETAFKAMMESFGWARRPMLERIHL
                     IRKDVPITMIYGANTWIDTSTGKKVKMQRPDSYVRDMEIEGASHHVYADQPHIFNAVV
                     EEICNSVD"
     exon            38..126
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /inference="alignment:Splign:2.1.0"
     exon            127..499
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /inference="alignment:Splign:2.1.0"
     exon            500..654
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /inference="alignment:Splign:2.1.0"
     exon            655..766
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /inference="alignment:Splign:2.1.0"
     exon            767..953
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /inference="alignment:Splign:2.1.0"
     exon            954..2351
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2328..2333
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /note="hexamer: AATAAA"
     polyA_site      2351
                     /gene="Abhd4"
                     /gene_synonym="1110035H23Rik; Abh4"
                     /note="major polyA site"
ORIGIN      
        1 agggcttgtt tactatggct gatgatctgg agcagcagcc tcagggctgg ctgagtagct
       61 ggctccccac ttggcgcccc acttccatgt ctcaactgaa gaatgtggaa gccaggatcc
      121 tccagtgtct ccagaacaag ttcctggccc gttatgtatc cctcccaaac cagaacaaga
      181 tctggacggt gactgtgagc ccagaacaaa aggatcgcac ccctctggtg atggtacatg
      241 gctttggggg cggtgtgggc ctctggatcc tcaacatgga ttcactgagt gcccgccgca
      301 cacttcatac ctttgatctg cttggttttg ggcgaagctc aaggccaaca ttcccaaggg
      361 acccagaagg agctgaagat gagtttgtgg cctcaataga gacatggcgg gagaccatgg
      421 gaatccccac catgatcctc ctggggcaca gtttgggagg attcctggcc acttcttact
      481 ctatcaagta ccctgaaaga gttaaacatc ttatcctggt ggatccatgg ggctttcccc
      541 tacgaccaac tgaccctagt gagatccgtg cacctccaac ctgggtcaag gctgtggcat
      601 ctgtcctggg acgttccaat ccactggctg ttcttcgagt ggctgggcct tgggggcctg
      661 ggctggtgca gagattccgt ccagacttca agcgcaagtt tgcagacttc tttgaggatg
      721 acaccatctc ggaatacatc taccactgca atgcacagaa tcccagtggg gaaacggcat
      781 tcaaagccat gatggagtcc tttggctggg cccggcgccc catgttggag cgaatccact
      841 taattcgaaa agatgtgccc atcaccatga tctatggggc caacacctgg atagatacca
      901 gcacagggaa gaaggtgaag atgcaaaggc cggattccta cgtccgagac atggagatcg
      961 agggcgcatc ccaccacgtc tatgctgacc agccacacat cttcaatgct gtggtagaag
     1021 agatctgcaa ctcagttgac tgagatgccc tgagagggag aggaggaagc cagagttgct
     1081 gtcatctgtg tccctgtctt agtcagaacc tctgtccttt cctcaccaac taacatgtgc
     1141 cagccaggca gagtctcggg ctgcccccaa gataggacca cagtgaacaa gagcacttcc
     1201 aatcccatgc agagggcaag aggcagaatc caccaaaggc cttgaactac ccatgctggg
     1261 gaaaagcata aagggctgcc tagtaccgcc cgttccctcc acgccaggtg gagactacaa
     1321 ggagactggg cgaaccacac cgtctccctg catggccttc ccttcctcta gacagaggag
     1381 ggctcccagc agcctttcta gtttggcagt gcgactgaga gaagattcca cacaggaaca
     1441 tctttcttct ccatcactcc acgcctccat ccaagctcag tttccactcg cccaccagaa
     1501 gcagctctag tcacctgcac aaccgcttcc atgaccttgg aggtatatcc caagctctca
     1561 ttggcagatg aggcaagaga taagggcagg atatagcaca tgatggcttc cagcagaact
     1621 gagtctatag cccaggtctg ctggcttcca gcacatgttg tacactaaga tttcatgcag
     1681 acaccattgc tgtggagccg attgaaattt aaccctgctc catattctaa cctgttatgc
     1741 aggtacactg ttatcttgcc ctgtgtctca atcctggttg tctaagttgg gacacctgag
     1801 tctcttcctc ttgttccttc cactatgagg ctctgagcct ctctgtgcct tgggccctaa
     1861 agccccatgt gtagtataga gcaaaggtga ttcctggtgg agaaggggaa aagcccttct
     1921 accctagagt catgcctgga tccacaactc cacaggtatc tgcagcattt tctacctggc
     1981 cctatcgctg agagcccctc cccatggctc actgaggagc actcagccag cagttctcta
     2041 tccttatcag ccacagaatg cttctcactg cccgaacctc tatgctctca tctgtcacca
     2101 tggcctccaa ctccttctgc ttcaaaatta tccatcagct ctctccagtg aatctcattc
     2161 caggtcctgc tctaaggctc tggggactag gctgggcctg gccccagaag ccgggcagtt
     2221 cctgcctcct tctccacttt tataaagcca agcctttccc cagaatagta ttaactcctg
     2281 gtgctttgta ctactggttc agcggcctcg ggcttctttt ctatattaat aaagaagagt
     2341 aaaaactgtc a
//
