LOCUS       NM_001163676            5504 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus ATP-binding cassette, sub-family C member 4 (Abcc4),
            transcript variant 3, mRNA.
ACCESSION   NM_001163676
VERSION     NM_001163676.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ranjit,S., Wang,Y., Zhu,J., Cheepala,S.B., Schuetz,E.G., Cho,W.J.,
            Xu,B., Robinson,C.G., Wu,G., Naren,A.P. and Schuetz,J.D.
  TITLE     ABCC4 impacts megakaryopoiesis and protects megakaryocytes against
            6-mercaptopurine induced cytotoxicity
  JOURNAL   Drug Resist Updat 72, 101017 (2024)
   PUBMED   37988981
  REMARK    GeneRIF: ABCC4 impacts megakaryopoiesis and protects megakaryocytes
            against 6-mercaptopurine induced cytotoxicity.
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Yerushalmi,G.M., Shuraki,B., Yung,Y., Maman,E., Baum,M.,
            Hennebold,J.D., Adashi,E.Y. and Hourvitz,A.
  TITLE     ABCC4 is a PGE2 efflux transporter in the ovarian follicle: A
            mediator of ovulation and a potential non-hormonal contraceptive
            target
  JOURNAL   FASEB J 37 (4), e22858 (2023)
   PUBMED   36943419
  REMARK    GeneRIF: ABCC4 is a PGE2 efflux transporter in the ovarian
            follicle: A mediator of ovulation and a potential non-hormonal
            contraceptive target.
REFERENCE   3  (bases 1 to 5504)
  AUTHORS   Belleville-Rolland,T., Leuci,A., Mansour,A., Decouture,B.,
            Martin,F., Poirault-Chassac,S., Rouaud,M., Guerineau,H., Dizier,B.,
            Pidard,D., Gaussem,P. and Bachelot-Loza,C.
  TITLE     Role of Membrane Lipid Rafts in MRP4 (ABCC4) Dependent Regulation
            of the cAMP Pathway in Blood Platelets
  JOURNAL   Thromb Haemost 121 (12), 1628-1636 (2021)
   PUBMED   33851387
  REMARK    GeneRIF: Role of Membrane Lipid Rafts in MRP4 (ABCC4) Dependent
            Regulation of the cAMP Pathway in Blood Platelets.
REFERENCE   4  (bases 1 to 5504)
  AUTHORS   Donepudi,A.C., Lee,Y., Lee,J.Y., Schuetz,J.D. and Manautou,J.E.
  TITLE     Multidrug resistance-associated protein 4 (Mrp4) is a novel genetic
            factor in the pathogenesis of obesity and diabetes
  JOURNAL   FASEB J 35 (2), e21304 (2021)
   PUBMED   33417247
REFERENCE   5  (bases 1 to 5504)
  AUTHORS   Chatterjee,S., Mukherjee,S., Sankara Sivaprasad,L.V.J., Naik,T.,
            Gautam,S.S., Murali,B.V., Hadambar,A.A., Gunti,G.R.,
            Kuchibhotla,V., Deyati,A., Basavanthappa,S., Ramarao,M.,
            Mariappan,T.T., Zinker,B.A., Zhang,Y., Sinz,M. and Shen,H.
  TITLE     Transporter Activity Changes in Nonalcoholic Steatohepatitis:
            Assessment with Plasma Coproporphyrin I and III
  JOURNAL   J Pharmacol Exp Ther 376 (1), 29-39 (2021)
   PUBMED   33127749
  REMARK    GeneRIF: Transporter Activity Changes in Nonalcoholic
            Steatohepatitis: Assessment with Plasma Coproporphyrin I and III.
REFERENCE   6  (bases 1 to 5504)
  AUTHORS   Assem,M., Schuetz,E.G., Leggas,M., Sun,D., Yasuda,K., Reid,G.,
            Zelcer,N., Adachi,M., Strom,S., Evans,R.M., Moore,D.D., Borst,P.
            and Schuetz,J.D.
  TITLE     Interactions between hepatic Mrp4 and Sult2a as revealed by the
            constitutive androstane receptor and Mrp4 knockout mice
  JOURNAL   J Biol Chem 279 (21), 22250-22257 (2004)
   PUBMED   15004017
  REMARK    GeneRIF: and Sult2a1 participate in an integrated pathway mediating
            elimination of sulfated steroid and bile acid metabolites from the
            liver
REFERENCE   7  (bases 1 to 5504)
  AUTHORS   Rius,M., Nies,A.T., Hummel-Eisenbeiss,J., Jedlitschky,G. and
            Keppler,D.
  TITLE     Cotransport of reduced glutathione with bile salts by MRP4 (ABCC4)
            localized to the basolateral hepatocyte membrane
  JOURNAL   Hepatology 38 (2), 374-384 (2003)
   PUBMED   12883481
  REMARK    GeneRIF: abcc4 can mediate the efflux of reduced glutathione from
            hepatocytes into blood by cotransport with monoanionic bile salts.
REFERENCE   8  (bases 1 to 5504)
  AUTHORS   Lamba,J.K., Adachi,M., Sun,D., Tammur,J., Schuetz,E.G.,
            Allikmets,R. and Schuetz,J.D.
  TITLE     Nonsense mediated decay downregulates conserved alternatively
            spliced ABCC4 transcripts bearing nonsense codons
  JOURNAL   Hum Mol Genet 12 (2), 99-109 (2003)
   PUBMED   12499391
REFERENCE   9  (bases 1 to 5504)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 5504)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167668.1, AC154419.2 and CT025694.11.
            
            Transcript Variant: This variant (3) lacks an in-frame exon in the
            coding region, compared to variant 1. The encoded isoform (2) is
            shorter, compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC171974.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-184               AC167668.1         99364-99547         c
            185-295             AC167668.1         62325-62435         c
            296-416             AC167668.1         61675-61795         c
            417-506             AC154419.2         32854-32943
            507-670             AC154419.2         33899-34062
            671-796             AC154419.2         35298-35423
            797-1046            AC154419.2         37340-37589
            1047-1148           AC154419.2         46051-46152
            1149-1238           AC154419.2         48586-48675
            1239-1430           AC154419.2         49719-49910
            1431-1525           AC154419.2         53229-53323
            1526-1612           AC154419.2         53518-53604
            1613-1709           AC154419.2         60788-60884
            1710-1919           AC154419.2         65518-65727
            1920-2060           AC154419.2         67407-67547
            2061-2098           AC154419.2         67865-67902
            2099-2193           AC154419.2         68267-68361
            2194-2339           AC154419.2         70133-70278
            2340-2340           AC154419.2         70279-70279
            2341-2420           CT025694.11        194501-194580       c
            2421-2571           CT025694.11        175209-175359       c
            2572-2691           CT025694.11        171075-171194       c
            2692-2802           CT025694.11        170184-170294       c
            2803-2903           CT025694.11        169168-169268       c
            2904-3095           CT025694.11        167429-167620       c
            3096-3251           CT025694.11        157650-157805       c
            3252-3341           CT025694.11        148810-148899       c
            3342-3514           CT025694.11        141925-142097       c
            3515-3620           CT025694.11        141183-141288       c
            3621-3755           CT025694.11        131716-131850       c
            3756-5504           CT025694.11        123909-125657       c
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 62.24 cM"
     gene            1..5504
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /note="ATP-binding cassette, sub-family C member 4"
                     /db_xref="GeneID:239273"
                     /db_xref="MGI:MGI:2443111"
     exon            1..184
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..3863
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /EC_number="7.6.2.2"
                     /EC_number="7.6.2.3"
                     /note="isoform 3 is encoded by transcript variant 3;
                     multidrug resistance-associated protein 4; ATP-binding
                     cassette, sub-family C (CFTR/MRP), member 4"
                     /codon_start=1
                     /product="ATP-binding cassette sub-family C member 4
                     isoform 3"
                     /protein_id="NP_001157148.1"
                     /db_xref="CCDS:CCDS49565.1"
                     /db_xref="GeneID:239273"
                     /db_xref="MGI:MGI:2443111"
                     /translation="MLPVHTEVKPNPLQDANLCSRVFFWWLNPLFKTGHKRRLEEDDM
                     FSVLPEDRSKHLGEELQRYWDKELLRAKKDSRKPSLTKAIIKCYWKSYLILGIFTLIE
                     ALRLSNSAMGKTTTGQIVNLLSNDVNKFDQVTIFLHFLWAGPLQAIAVTVLLWVEIGI
                     SCLAGLAVLVILLPLQSCIGKLFSSLRSKTAAFTDARIRTMNEVITGMRIIKMYAWEK
                     SFADLIANLRKKEISKILGSSYLRGMNMASFFIANKVILFVTFTSYVLLGNEITASHV
                     FVAMTLYGAVRLTVTLFFPSAIERGSEAIVSIRRIKNFLLLDELPQRKAHVPSDGKAI
                     VHVQDFTAFWDKALDSPTLQGLSFIARPGELLAVVGPVGAGKSSLLSAVLGELPPASG
                     LVSVHGRIAYVSQQPWVFSGTVRSNILFGKKYEKERYEKVIKACALKKDLQLLEDGDL
                     TVIGDRGATLSGGQKARVNLARAVYQDADIYLLDDPLSAVDAEVGKHLFQLCICQALH
                     EKITILVTHQLQYLKAASHILILKDGEMVQKGTYTEFLKSGVDFGSLLKKENEEAEPS
                     TAPGTPTLRKRTFSEASIWSQQSSRPSLKDGAPEGQDAENTQAVQPEESRSEGRIGFK
                     AYKNYFSAGASWFFIIFLVLLNMVGQVFYVLQDWWLSHWANKQGALNNTRNANGNITE
                     TLDLSWYLGIYAGLTAVTVLFGIARSLLVFYILVNASQTLHNRMFESILKAPVLFFDR
                     NPIGRILNRFSKDIGHMDDLLPLTFLDFIQTLLLVVSVIAVAAAVIPWILIPLVPLSV
                     VFLVLRRYFLETSRDVKRLESTTRSPVFSHLSSSLQGLWTIRAYKAEERCQELFDAHQ
                     DLHSEAWFLFLTTSRWFAVRLDAICAIFVIVVAFGSLVLAKTLNAGQVGLALSYALTL
                     MGMFQWSVRQSAEVENMMISVERVIEYTDLEKEAPWECKKRPPPGWPHEGVIVFDNVN
                     FTYSLDGPLVLKHLTALIKSREKVGIVGRTGAGKSSLISALFRLSEPEGKIWIDKILT
                     TEIGLHDLRKKMSIIPQEPVLFTGTMRKNLDPFNEHTDEELWRALEEVQLKEAIEDLP
                     GKMDTELAESGSNFSVGQRQLVCLARAILKNNRILIIDEATANVDPRTDELIQQKIRE
                     KFAQCTVLTIAHRLNTIIDSDKIMVLDSGRLKEYDEPYVLLQNPESLFYKMVQQLGKG
                     EAAALTETAKQVYFRRNYPDITFTSPAVMNTSNGQPSALTIFETAL"
     exon            185..295
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            296..416
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            417..506
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            507..670
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            671..796
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            797..1046
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1148
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1238
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1430
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1525
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1612
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1709
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1919
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2060
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2098
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2193
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2194..2340
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2341..2420
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2571
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2572..2691
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2692..2802
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2903
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2904..3095
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3096..3251
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3252..3341
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3514
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3515..3620
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3621..3755
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3756..5504
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5059..5064
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /note="hexamer: AATAAA"
     polyA_site      5078
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /note="major polyA site"
     regulatory      5403..5408
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
                     /note="hexamer: ATTAAA"
     polyA_site      5424
                     /gene="Abcc4"
                     /gene_synonym="ABCC4-N1; D630049P08Rik; MOATB; MRP4"
ORIGIN      
        1 ggttcccgga agccgctgct tgcctgcgag gctccggccg aggccggagc tcgagcatcc
       61 tcacccgcgc ttcccggagc cgccgcccgg ccgcgcgcac cgcgggcgag atgctgccgg
      121 tgcacaccga ggtgaaaccc aacccgctgc aggacgccaa cctctgctcg cgcgtgttct
      181 tctggtggct caacccgctg tttaaaactg gtcataagcg gagactggaa gaagatgaca
      241 tgttctcagt gcttccagaa gatcgctcaa agcacctcgg agaggagctt caacggtact
      301 gggataaaga acttctgcga gccaagaagg actcgaggaa gccctcctta acaaaggcaa
      361 tcataaagtg ttactggaag tcttacctga ttttgggaat ttttacgtta attgaggcac
      421 tccggttaag taactcggcc atggggaaga caaccacagg ccagatagtt aacctgctgt
      481 ccaacgacgt gaacaaattc gaccaagtga caatcttctt gcactttctg tgggcagggc
      541 cgctgcaggc catcgcggta accgtcctcc tctgggtgga gataggaatc tcctgcctgg
      601 cgggcttggc cgttctggtt attcttctgc ctctgcaaag ctgcatcggg aagctgttct
      661 cgtcactgcg gagtaaaact gcggctttca cggatgccag gatccggacc atgaatgaag
      721 tcataacagg catgaggata ataaagatgt atgcgtggga gaaatcgttt gctgacctca
      781 ttgccaatct gagaaagaag gagatttcca agattctggg cagctcctac ctcagaggga
      841 tgaacatggc gtcgtttttc atcgcaaaca aagtcatcct gttcgtgacc ttcactagct
      901 acgtgctgct tggcaatgag attacagcta gccacgtgtt tgtggccatg actctgtacg
      961 gtgccgttcg gttgacagtc accctcttct tcccgtcagc cattgagaga gggtcagagg
     1021 ccatcgtcag cattcggagg atcaagaact ttctgttact cgatgaacta ccacagcgca
     1081 aagcccatgt accatctgat ggcaaagcca ttgtccacgt gcaagatttc accgctttct
     1141 gggacaaggc actagacagt ccaaccctgc aaggtctttc ctttattgcc aggcctggtg
     1201 agttgttagc cgtggttggc ccagttggag caggcaagtc gtcgctgttg agcgcagtgc
     1261 tgggtgagct gcctcctgcc agcgggctgg tcagcgtgca cgggaggatc gcctacgttt
     1321 ctcagcagcc ctgggtgttc tcgggcaccg tgaggagcaa tattttattt gggaagaaat
     1381 atgagaagga gcgatatgag aaagtgatca aggcctgtgc tctgaagaag gacctgcagc
     1441 ttctggagga cggggatctg acggttatag gagaccgggg agccacgctg agtggaggcc
     1501 agaaagctcg ggtgaacctg gcacgggccg tctaccagga cgccgacatc tacctccttg
     1561 atgatccgct cagcgctgtc gatgcagaag tgggcaagca cctgttccaa ctgtgtatct
     1621 gtcaggcgtt gcacgagaag atcaccattt tagtgactca ccagttacag tacctcaaag
     1681 ctgcaagcca catcctcata ctcaaagatg gtgagatggt gcagaagggg acttacacgg
     1741 agtttctgaa atctggtgta gattttggct ccctgttaaa gaaagaaaac gaggaagcag
     1801 agccctccac agccccagga accccgacac tcaggaaacg aaccttctcc gaggcctcaa
     1861 tttggtctca gcagtcatcc agaccctcgt tgaaagacgg ggccccagag ggccaagacg
     1921 cagagaacac gcaggcagtg caacccgagg agagccgttc ggaagggaga atcggcttca
     1981 aggcctacaa gaattacttc tcggcgggcg catcctggtt cttcatcatt ttccttgtgc
     2041 tgcttaacat ggtgggccag gttttctatg ttcttcagga ctggtggctt tcccactggg
     2101 cgaacaagca aggtgcactg aacaacacca gaaatgcgaa tggaaatata acggagaccc
     2161 tagacctcag ctggtactta ggaatttacg caggtctaac tgcggtcacc gtcctttttg
     2221 gcatagcgag atccctactg gtgttctata tccttgtgaa cgcttcccag actttgcaca
     2281 acaggatgtt tgagtccata ctgaaggctc ccgtgttgtt cttcgacaga aatccaatcg
     2341 ggaggatttt aaatcgtttc tccaaagaca tcggacacat ggatgatttg cttcccctga
     2401 cgttcctgga cttcatccag acgttgctcc tcgtcgtaag tgtgatcgct gtggccgcgg
     2461 ccgtgatccc ttggatcctc ataccattgg ttccgctctc agtcgtcttc ctggttcttc
     2521 ggagatactt cttagagacg tcacgggatg tcaagcgcct ggaatccaca acacggagcc
     2581 cggtattctc ccatttatcg tcctccctcc agggactctg gaccatccgg gcttacaaag
     2641 ctgaggagag gtgtcaggag ctgtttgatg cacaccagga cttgcattca gaggcttggt
     2701 tcttgttcct gacgacatcg agatggttcg ctgtgcgtct ggacgccatc tgcgccatct
     2761 ttgtaatcgt cgttgccttc gggtcccttg ttctggcgaa gactttgaat gctgggcagg
     2821 ttggcctggc cttgtcctac gccctcacac tcatggggat gttccagtgg tctgtgcgac
     2881 agagcgccga agtagagaat atgatgattt cagtggagag agtgattgag tacacggacc
     2941 tagagaagga ggcgccttgg gagtgcaaga agcgcccacc cccaggctgg ccccacgagg
     3001 gagtcatcgt cttcgacaat gtgaacttca cctacagctt agatgggcct ctggttctga
     3061 agcacctgac tgcgctcatc aagtccaggg aaaaggttgg aattgtgggc agaaccggag
     3121 ctgggaaaag ctccctcatc tcggccctct tcaggctgtc agaacccgag gggaaaatct
     3181 ggatcgataa gatcttgaca accgaaattg ggcttcacga cttaaggaag aaaatgtcaa
     3241 tcataccaca ggaacctgtt ctgttcactg gaaccatgag gaaaaacctg gaccccttca
     3301 atgagcacac ggacgaggag ctgtggaggg ccttggagga ggtacaactt aaagaggcca
     3361 ttgaagatct tcctggaaaa atggatactg aattagcaga atctggatcc aatttcagtg
     3421 ttggacagag acagttagtg tgccttgcaa gggccattct aaagaataac cgaatactga
     3481 tcattgatga agcaactgca aatgtggacc caagaacgga tgagttaata caacagaaga
     3541 tccgggagaa gtttgcccag tgcacagtgc tcaccattgc tcacagactg aacaccatca
     3601 ttgacagtga caagataatg gttttggatt caggaagact gaaagaatat gatgagccgt
     3661 atgtcttgct gcagaatcca gagagcctct tttacaagat ggttcagcaa ctgggcaagg
     3721 gcgaagccgc tgccctcacc gaaacagcaa aacaggtata cttcagacgg aattacccag
     3781 atattacatt caccagcccc gcggttatga acacctccaa tggacagccc tcggccttaa
     3841 caatatttga aacagcattg tgaccaccag gacatcatcg taaagtctgt cttgaaggtg
     3901 ttccccaccc cgcccagggg cttgcacttg gaggactaca gcacacctgg tttcctttct
     3961 ttctgccaca gatccttcac agacgagaga ctgttgtctt tggatttttc cctccgactt
     4021 tactctgtga tttcaggttc taacaacatg gcctgtcgtt ttattttaaa agttcctgct
     4081 gttctttaca aatccagttc tgaggtgcag gccaccaaca gcagcgtcta ccaggttttg
     4141 tgccttaaca gtctcaaggg ctgaagccca ttcattttat aagctgtcac catgggctgc
     4201 tgcttcctct tgccttaaaa gtacactctc caggtgtgtc agggactctg tttacttgaa
     4261 gactgtgtgc agttgccagt tcgtctccct gctgtctttg agctaggact cataatttcc
     4321 ctacaaaggt ctctggttaa aatagaatag gtagaattaa caagaaccac aacaacaacc
     4381 cccccaaaaa ataatccata taatttaagt atattgctgg agagagacta tcccaccacc
     4441 tatacatcta caaagatata tgtctaaagt tggaaaatca acttctgctt tctcagaatg
     4501 ggagaccgag cgcaggttag gcacccctcc cccttgcatc tgtgagtccc tctgggcggc
     4561 tcgtgtactg atcggatctg tgagtccctc tgggcggctc gtgtactgat cggatcggca
     4621 ccatgacact tcccctagac tgtgactccc aaatgcataa tattaatctc atttggcttc
     4681 tggtttacag aaggtaaagc tcctattggc tgtgagccgt tggtgaaatc ctttcacatc
     4741 gacaccaaaa gctctcccca gctagtgaaa acctgaacat cgttagagtc accgacttta
     4801 catgctcaga atgctgtgca caagccacgc tttctaaagc ccatccagat tagtttagct
     4861 ctgaccacta atggcaggtc ttaaggacct tgtgattagt caatcaacaa accacaacac
     4921 ttcctaggac ctgttgtact ccgtgtaact ataggtctga atattttctg ggtggtggtg
     4981 gcaagggtct atgtcagggt tcaagttact aaaggtttta ctgtcataat ttaaagggga
     5041 ctcttcacga cccgtcacaa taaaccattc tcaagttatc tatgtgtgtg tgtatatata
     5101 tctcaggttt ttagactggt tgacagaaca aagcctgttt taacagtgga accttaaaga
     5161 gcacacctac accaaaccac caaaaccacg ccgagcacag tgactgtttg actcttggta
     5221 ttcagaagtc tgtaatgaat gtaattatgt ttaggccagt aaatgatgta gattcgtcct
     5281 ttattggtag tcgcagttga tatttcaaga gagtgagatg ccatgctgac aatattaaga
     5341 tctatttgaa tatttagaaa tgtcttactg tatatatgtt tatgtttatt tggtccttag
     5401 agattaaaag cggtcataca agtatgttcc tatttcattt ctatttttca caccatcaca
     5461 ggacaaacca aagtcaactg aaataaatga cagtttccat cccc
//
