LOCUS       NM_013454              10260 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus ATP-binding cassette, sub-family A member 1 (Abca1),
            mRNA.
ACCESSION   NM_013454
VERSION     NM_013454.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Dahik,V.D., Kc,P., Materne,C., Reydellet,C., Lhomme,M.,
            Cruciani-Guglielmacci,C., Denom,J., Bun,E., Ponnaiah,M.,
            Deknuydt,F., Frisdal,E., Hardy,L.M., Durand,H., Guillas,I.,
            Lesnik,P., Gudelj,I., Lauc,G., Guerin,M., Kontush,A., Soprani,A.,
            Magnan,C., Diedisheim,M., Bluteau,O., Venteclef,N. and Le Goff,W.
  TITLE     ABCG1 orchestrates adipose tissue macrophage plasticity and insulin
            resistance in obesity by rewiring saturated fatty acid pools
  JOURNAL   Sci Transl Med 16 (777), eadi6682 (2024)
   PUBMED   39661702
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   Yamauchi,Y., Abe-Dohmae,S., Iwamoto,N., Sato,R. and Yokoyama,S.
  TITLE     ABCA1 deficiency causes tissue-specific dysregulation of the SREBP2
            pathway in mice
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1869 (8), 159546 (2024)
   PUBMED   39089642
  REMARK    GeneRIF: ABCA1 deficiency causes tissue-specific dysregulation of
            the SREBP2 pathway in mice.
REFERENCE   3  (bases 1 to 10260)
  AUTHORS   Kloosterman,D.J., Erbani,J., Boon,M., Farber,M., Handgraaf,S.M.,
            Ando-Kuri,M., Sanchez-Lopez,E., Fontein,B., Mertz,M., Nieuwland,M.,
            Liu,N.Q., Forn-Cuni,G., van der Wel,N.N., Grootemaat,A.E.,
            Reinalda,L., van Kasteren,S.I., de Wit,E., Ruffell,B.,
            Snaar-Jagalska,E., Petrecca,K., Brandsma,D., Kros,A., Giera,M. and
            Akkari,L.
  TITLE     Macrophage-mediated myelin recycling fuels brain cancer malignancy
  JOURNAL   Cell 187 (19), 5336-5356 (2024)
   PUBMED   39137777
REFERENCE   4  (bases 1 to 10260)
  AUTHORS   Ou,Z., Cheng,Y., Ma,H., Chen,K., Lin,Q., Chen,J., Guo,R., Huang,Z.,
            Cheng,Q., Alaeiilkhchi,N., Zhu,Q., Huang,Z. and Jiang,H.
  TITLE     miR-223 accelerates lipid droplets clearance in microglia following
            spinal cord injury by upregulating ABCA1
  JOURNAL   J Transl Med 22 (1), 659 (2024)
   PUBMED   39010173
  REMARK    GeneRIF: miR-223 accelerates lipid droplets clearance in microglia
            following spinal cord injury by upregulating ABCA1.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10260)
  AUTHORS   Wang,X., Cheng,Z., Tai,W., Shi,M., Ayazi,M., Liu,Y., Sun,L., Yu,C.,
            Fan,Z., Guo,B., He,X., Sun,D., Young,W. and Ren,Y.
  TITLE     Targeting foamy macrophages by manipulating ABCA1 expression to
            facilitate lesion healing in the injured spinal cord
  JOURNAL   Brain Behav Immun 119, 431-453 (2024)
   PUBMED   38636566
  REMARK    GeneRIF: Targeting foamy macrophages by manipulating ABCA1
            expression to facilitate lesion healing in the injured spinal cord.
REFERENCE   6  (bases 1 to 10260)
  AUTHORS   Coveney,E., Clary,B., Iacobucci,M., Philip,R. and Lyerly,K.
  TITLE     Active immunotherapy with transiently transfected
            cytokine-secreting tumor cells inhibits breast cancer metastases in
            tumor-bearing animals
  JOURNAL   Surgery 120 (2), 265-72 (1996)
   PUBMED   8751592
REFERENCE   7  (bases 1 to 10260)
  AUTHORS   Luciani,M.F. and Chimini,G.
  TITLE     The ATP binding cassette transporter ABC1, is required for the
            engulfment of corpses generated by apoptotic cell death
  JOURNAL   EMBO J 15 (2), 226-235 (1996)
   PUBMED   8617198
REFERENCE   8  (bases 1 to 10260)
  AUTHORS   Pilz,A., Woodward,K., Povey,S. and Abbott,C.
  TITLE     Comparative mapping of 50 human chromosome 9 loci in the laboratory
            mouse
  JOURNAL   Genomics 25 (1), 139-149 (1995)
   PUBMED   7774911
REFERENCE   9  (bases 1 to 10260)
  AUTHORS   Luciani,M.F., Denizot,F., Savary,S., Mattei,M.G. and Chimini,G.
  TITLE     Cloning of two novel ABC transporters mapping on human chromosome 9
  JOURNAL   Genomics 21 (1), 150-159 (1994)
   PUBMED   8088782
REFERENCE   10 (bases 1 to 10260)
  AUTHORS   Higgins,C.F.
  TITLE     ABC transporters: from microorganisms to man
  JOURNAL   Annu Rev Cell Biol 8, 67-113 (1992)
   PUBMED   1282354
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK051920.1, AL807243.9, BP761367.1, AL772397.3, CD351847.1,
            CF745062.1, CN702062.1, CD351490.1, CF734665.1, AK019548.1 and
            AK045442.1.
            
            On Mar 25, 2006 this sequence version replaced NM_013454.2.
            
            Summary: The membrane-associated protein encoded by this gene is a
            member of the superfamily of ATP-binding cassette (ABC)
            transporters. ABC proteins transport various molecules across
            extra- and intracellular membranes. ABC genes are divided into
            seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20,
            White). This protein is a member of the ABC1 subfamily. Members of
            the ABC1 subfamily comprise the only major ABC subfamily found
            exclusively in multicellular eukaryotes. In humans, this protein
            functions as a cholesterol efflux pump in the cellular lipid
            removal pathway. Mutations in the human gene have been associated
            with Tangier's disease and familial high-density lipoprotein
            deficiency. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: X75926.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMN00849374,
                                    SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1631              AK051920.1         2-1632
            1632-1829           AL807243.9         8318-8515           c
            1830-4782           AK051920.1         1831-4783
            4783-5018           BP761367.1         168-403
            5019-5093           AL772397.3         201932-202006       c
            5094-5787           CD351847.1         61-754
            5788-6247           CF745062.1         189-648
            6248-6713           CN702062.1         137-602
            6714-7006           CD351490.1         337-629
            7007-7462           CF734665.1         150-605
            7463-8880           AK019548.1         267-1684
            8881-10140          AK045442.1         947-2206
            10141-10260         AK019548.1         2945-3064
FEATURES             Location/Qualifiers
     source          1..10260
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 28.57 cM"
     gene            1..10260
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /note="ATP-binding cassette, sub-family A member 1"
                     /db_xref="GeneID:11303"
                     /db_xref="MGI:MGI:99607"
     exon            1..220
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            221..386
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             321..7106
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /EC_number="7.6.2.1"
                     /note="ATP-binding cassette transporter 1; ATP-binding
                     cassette 1; ATP-binding cassette, sub-family A (ABC1),
                     member 1"
                     /codon_start=1
                     /product="phospholipid-transporting ATPase ABCA1"
                     /protein_id="NP_038482.3"
                     /db_xref="CCDS:CCDS18187.1"
                     /db_xref="GeneID:11303"
                     /db_xref="MGI:MGI:99607"
                     /translation="MACWPQLRLLLWKNLTFRRRQTCQLLLEVAWPLFIFLILISVRL
                     SYPPYEQHECHFPNKAMPSAGTLPWVQGIICNANNPCFRYPTPGEAPGVVGNFNKSIV
                     SRLFSDAQRLLLYSQRDTSIKDMHKVLRMLRQIKHPNSNLKLQDFLVDNETFSGFLQH
                     NLSLPRSTVDSLLQANVGLQKVFLQGYQLHLASLCNGSKLEEIIQLGDAEVSALCGLP
                     RKKLDAAERVLRYNMDILKPVVTKLNSTSHLPTQHLAEATTVLLDSLGGLAQELFSTK
                     SWSDMRQEVMFLTNVNSSSSSTQIYQAVSRIVCGHPEGGGLKIKSLNWYEDNNYKALF
                     GGNNTEEDVDTFYDNSTTPYCNDLMKNLESSPLSRIIWKALKPLLVGKILYTPDTPAT
                     RQVMAEVNKTFQELAVFHDLEGMWEELSPQIWTFMENSQEMDLVRTLLDSRGNDQFWE
                     QKLDGLDWTAQDIMAFLAKNPEDVQSPNGSVYTWREAFNETNQAIQTISRFMECVNLN
                     KLEPIPTEVRLINKSMELLDERKFWAGIVFTGITPDSVELPHHVKYKIRMDIDNVERT
                     NKIKDGYWDPGPRADPFEDMRYVWGGFAYLQDVVEQAIIRVLTGSEKKTGVYVQQMPY
                     PCYVDDIFLRVMSRSMPLFMTLAWIYSVAVIIKSIVYEKEARLKETMRIMGLDNGILW
                     FSWFVSSLIPLLVSAGLLVVILKLGNLLPYSDPSVVFVFLSVFAMVTILQCFLISTLF
                     SRANLAAACGGIIYFTLYLPYVLCVAWQDYVGFSIKIFASLLSPVAFGFGCEYFALFE
                     EQGIGVQWDNLFESPVEEDGFNLTTAVSMMLFDTFLYGVMTWYIEAVFPGQYGIPRPW
                     YFPCTKSYWFGEEIDEKSHPGSSQKGVSEICMEEEPTHLRLGVSIQNLVKVYRDGMKV
                     AVDGLALNFYEGQITSFLGHNGAGKTTTMSILTGLFPPTSGTAYILGKDIRSEMSSIR
                     QNLGVCPQHNVLFDMLTVEEHIWFYARLKGLSEKHVKAEMEQMALDVGLPPSKLKSKT
                     SQLSGGMQRKLSVALAFVGGSKVVILDEPTAGVDPYSRRGIWELLLKYRQGRTIILST
                     HHMDEADILGDRIAIISHGKLCCVGSSLFLKNQLGTGYYLTLVKKDVESSLSSCRNSS
                     STVSCLKKEDSVSQSSSDAGLGSDHESDTLTIDVSAISNLIRKHVSEARLVEDIGHEL
                     TYVLPYEAAKEGAFVELFHEIDDRLSDLGISSYGISETTLEEIFLKVAEESGVDAETS
                     DGTLPARRNRRAFGDKQSCLHPFTEDDAVDPNDSDIDPESRETDLLSGMDGKGSYQLK
                     GWKLTQQQFVALLWKRLLIARRSRKGFFAQIVLPAVFVCIALVFSLIVPPFGKYPSLE
                     LQPWMYNEQYTFVSNDAPEDMGTQELLNALTKDPGFGTRCMEGNPIPDTPCLAGEEDW
                     TISPVPQSIVDLFQNGNWTMKNPSPACQCSSDKIKKMLPVCPPGAGGLPPPQRKQKTA
                     DILQNLTGRNISDYLVKTYVQIIAKSLKNKIWVNEFRYGGFSLGVSNSQALPPSHEVN
                     DAIKQMKKLLKLTKDSSADRFLSSLGRFMAGLDTKNNVKVWFNNKGWHAISSFLNVIN
                     NAILRANLQKGENPSQYGITAFNHPLNLTKQQLSEVALMTTSVDVLVSICVIFAMSFV
                     PASFVVFLIQERVSKAKHLQFISGVKPVIYWLSNFVWDMCNYVVPATLVIIIFICFQQ
                     KSYVSSTNLPVLALLLLLYGWSITPLMYPASFVFKIPSTAYVVLTSVNLFIGINGSVA
                     TFVLELFTNNKLNDINDILKSVFLIFPHFCLGRGLIDMVKNQAMADALERFGENRFVS
                     PLSWDLVGRNLFAMAVEGVVFFLITVLIQYRFFIRPRPVKAKLPPLNDEDEDVRRERQ
                     RILDGGGQNDILEIKELTKIYRRKRKPAVDRICIGIPPGECFGLLGVNGAGKSTTFKM
                     LTGDTPVTRGDAFLNKNSILSNIHEVHQNMGYCPQFDAITELLTGREHVEFFALLRGV
                     PEKEVGKVGEWAIRKLGLVKYGEKYASNYSGGNKRKLSTAMALIGGPPVVFLDEPTTG
                     MDPKARRFLWNCALSIVKEGRSVVLTSHSMEECEALCTRMAIMVNGRFRCLGSVQHLK
                     NRFGDGYTIVVRIAGSNPDLKPVQEFFGLAFPGSVLKEKHRNMLQYQLPSSLSSLARI
                     FSILSQSKKRLHIEDYSVSQTTLDQVFVNFAKDQSDDDHLKDLSLHKNQTVVDVAVLT
                     SFLQDEKVKESYV"
     exon            387..480
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            481..622
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            623..741
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            742..863
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            864..1040
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1133
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1374
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1514
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1631
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1829
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..2035
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2212
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2435
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2657
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2658..2862
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2863..2976
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..3148
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3280
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3281..3423
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3424..3561
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3562..3782
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3783..3855
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3856..4058
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            4059..4107
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            4108..4221
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            4222..4370
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            4371..4495
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            4496..4594
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            4595..4784
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            4785..4879
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            4880..4912
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            4913..5018
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            5019..5093
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            5094..5263
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            5264..5441
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            5442..5557
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            5558..5702
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            5703..5826
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            5827..5956
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            5957..6077
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6078..6140
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6141..6247
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6248..6389
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6390..6524
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6525..6628
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6629..6721
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6722..6965
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6966..10260
                     /gene="Abca1"
                     /gene_synonym="ABC-1; Abc1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtaattccg agggcgagcg agcgggccgg gaccggcaga gcccacttct ctccgcggcg
       61 cagcgcaaag ctgggcaggg ggcgccgcgg gacccgcgca accacagccg gcttggggag
      121 ctgctctgct ccctgtttcc ccccactttt ttcttcccct ttctggaagg gtttgtgcag
      181 gggtagggaa aacagactca aacagcaaag tggaaaacag ttaatgacca gccacagagt
      241 cacagctctg tgctctggct gctccctcca gggctctcga gccgcagacg caggtcgctg
      301 tgggtgccgg ctgtggtgac atggcttgtt ggcctcagtt aaggctgctg ctgtggaaga
      361 atctgacatt tcgaaggaga caaacatgtc agctgttact ggaagtggcc tggcctctct
      421 ttatcttcct gatcctgatc tctgtacgcc tgagctaccc accctacgaa caacatgagt
      481 gccactttcc gaataaagcc atgccgtctg caggaaccct cccctgggta caggggatta
      541 tctgtaatgc caacaacccc tgcttccgtt atccaactcc cggcgaggct cccggtgttg
      601 ttggaaactt taacaagtcc atcgtgtctc gcctgttctc agacgctcag aggcttcttc
      661 tgtacagcca aagagatacc agcattaagg acatgcacaa ggtcctgaga atgttacggc
      721 agatcaagca tcccaactca aatttgaagc tccaggattt tctggtggac aatgaaacat
      781 tctctggatt cctgcagcac aatttgtccc ttccaagatc tactgtggac agcctgctgc
      841 aggcgaatgt tggtctccag aaggtatttt tgcaaggcta ccaattacat ttggccagtc
      901 tgtgtaacgg atcaaaatta gaagaaatta ttcagcttgg tgatgcggaa gtttctgccc
      961 tctgtggtct accgaggaag aagctcgatg cagccgagag agtactgcgc tacaacatgg
     1021 acatcctgaa gccagttgtg acaaaactaa attccacatc tcatctcccg acccagcatc
     1081 tggctgaagc caccacagtg ttgcttgaca gcttgggggg cctggcccaa gagctgttca
     1141 gcacaaagag ctggagcgac atgcggcagg aggtgatgtt tctgaccaac gtgaacagct
     1201 ccagctcctc cacccagatc taccaggcag tgtcccgcat cgtgtgtggt cacccagagg
     1261 gtgggggcct gaagatcaag tccctcaact ggtacgagga taacaactac aaagccctct
     1321 ttggagggaa taacaccgag gaagacgtgg acaccttcta tgacaattct acaactcctt
     1381 attgcaatga tttgatgaag aacttggagt ctagtcctct ttctcgaatt atttggaagg
     1441 cactcaagcc actgcttgtt ggaaagattc tctatacacc tgacacacca gctacaaggc
     1501 aggtcatggc tgaggtgaac aagacctttc aggagttggc tgtgttccat gacctggagg
     1561 gcatgtggga agaactcagc ccccaaattt ggaccttcat ggagaacagc caagagatgg
     1621 accttgtccg gacgctgtta gacagcagag gcaatgacca gttttgggaa cagaagttgg
     1681 atggattaga ttggactgcc caagacatca tggcgtttct ggccaagaac ccagaagatg
     1741 ttcagtcccc aaatggctct gtgtatacct ggagagaagc tttcaatgag accaaccagg
     1801 caatccagac gatatctcga ttcatggagt gtgtcaacct gaacaagctg gaacccattc
     1861 cgacagaagt caggctcatc aacaagtcca tggagctgct ggacgagagg aagttctggg
     1921 ctggcatcgt gttcacaggc atcactccag atagtgtgga gctgccccat catgtaaagt
     1981 acaagatccg gatggacatt gacaacgtgg agagaactaa taagatcaag gatgggtact
     2041 gggaccctgg tcctcgggct gacccttttg aagatatgcg ctatgtctgg ggcggcttcg
     2101 cctacttgca ggatgtggtg gaacaggcca tcatcagagt gctgacggga tctgagaaga
     2161 aaacgggtgt ctacgtgcaa cagatgccct acccctgtta tgttgatgac atttttctgc
     2221 gggtcatgag ccggtcaatg cccctcttca tgactctagc ctggatctac tctgtcgctg
     2281 tgatcatcaa gagcattgtg tatgagaagg aggctcggct gaaggagacc atgcggatca
     2341 tgggtctgga caatggcatc ctctggttta gctggtttgt tagcagcctc atccctctgc
     2401 ttgtgagcgc tggcctgctg gtggtcatct tgaagttagg aaacctgctg ccctatagtg
     2461 accccagcgt ggtgttcgtc ttcctgtctg tgtttgccat ggtgaccatc ctacagtgct
     2521 tcctcattag cacgctcttc tcccgtgcca acctggcagc agcctgtggg ggcatcatct
     2581 acttcacgct gtacctgccc tatgtgctgt gcgtagcctg gcaggactat gtgggcttct
     2641 ccatcaagat ctttgctagc ctgctgtctc ctgtggcttt tggattcggc tgtgagtatt
     2701 tcgccctttt cgaggagcaa ggtatcgggg tccaatggga caatctcttt gagagcccgg
     2761 tggaggagga cggcttcaat ctcaccactg cagtgtccat gatgctcttt gacacctttc
     2821 tctatggcgt gatgacatgg tacatcgaag ccgtctttcc aggacagtat ggaattccca
     2881 ggccctggta ttttccttgt accaagtcat actggtttgg tgaggaaatt gatgagaaga
     2941 gccaccctgg ttccagccag aagggagtgt cagaaatctg catggaagag gaacccactc
     3001 atctgaggct gggggtgtcc attcagaacc tggtgaaggt ttaccgagat ggcatgaagg
     3061 ttgctgtgga tggcttggcg ctcaactttt acgaaggcca gattacctcc ttcctgggcc
     3121 acaatggagc agggaagacc accaccatgt caatactgac tgggctgttt cccccaactt
     3181 ctggcacggc ctacatcctg gggaaggaca ttcgctcgga gatgagctcc atccggcaga
     3241 acctgggagt ctgtccccag cataatgtgc tgtttgacat gctgactgtc gaagagcaca
     3301 tctggttcta tgcgcgccta aaggggctct cagagaagca cgtgaaagca gagatggagc
     3361 agatggccct ggatgttggc ttacccccga gcaagctgaa aagcaaaacg agtcagctct
     3421 caggtgggat gcagagaaag ctgtctgtgg ccttggcctt cgtgggtgga tccaaggttg
     3481 tcattctgga cgagcccaca gccggggtgg acccgtactc tcgcagggga atatgggaac
     3541 tcctgctaaa ataccggcaa ggccgcacca ttattttgtc tacacaccac atggacgaag
     3601 ctgacatcct tggggacaga attgccatca tttcccatgg gaagctgtgt tgtgtgggct
     3661 cctccctgtt tttgaaaaac cagttgggaa cgggttacta tctgaccctg gttaagaaag
     3721 atgtggaatc gtccctcagt tcctgcagaa acagtagcag caccgtgtct tgtctgaaaa
     3781 aggaggacag tgtttctcag agcagttctg atgctggcct gggcagcgac catgaaagtg
     3841 acacgctgac catcgatgtc tctgctatct ccaacctcat caggaagcac gtgtctgaag
     3901 cccggctggt ggaggacatt gggcacgagc tgacctatgt gctgccgtac gaagccgcga
     3961 aggagggagc ctttgtggaa ctcttccatg agattgatga ccggctctca gacctgggca
     4021 tctccagtta tggcatctcg gagaccaccc tggaagaaat attcctcaaa gtggctgaag
     4081 agagcggggt ggatgctgag acctcagatg gtactttgcc agcaagacga aacagacggg
     4141 ccttcgggga caagcagagc tgtctgcacc catttacgga agatgatgct gttgatccca
     4201 atgactctga catagaccca gaatccaggg agaccgacct gctcagtggg atggacggca
     4261 aaggctccta ccagctgaag ggctggaaac tcacccagca acagtttgtg gcccttttgt
     4321 ggaagaggct gctgattgcc agacggagcc ggaagggttt ctttgctcag attgtcctgc
     4381 cagctgtctt tgtttgcatt gccctggtct tcagcctgat tgtgccaccc tttggcaagt
     4441 accccagcct ggaacttcag ccctggatgt ataatgagca gtatacattt gtcagtaatg
     4501 atgctcccga ggacatgggc acccaggaac tcctgaatgc tctgaccaaa gatccaggct
     4561 ttgggacccg ctgtatggaa ggaaacccaa tcccagatac cccttgcttg gctggggagg
     4621 aggactggac catcagcccc gtcccccaga gcatcgtgga cctcttccag aatggaaact
     4681 ggaccatgaa gaacccctca cctgcgtgcc agtgtagcag tgacaaaatc aagaagatgc
     4741 tgcctgtgtg tcccccaggg gcaggggggc tgccacctcc tcagagaaaa cagaaaaccg
     4801 cagacatcct tcagaatctg acaggaagaa atatttcaga ttacctggtg aagacgtacg
     4861 tgcagatcat agcaaagagc ttaaagaata agatctgggt gaacgagttt cggtatggcg
     4921 ggttttccct gggtgtcagt aattctcaag cacttcctcc gagccatgaa gttaatgatg
     4981 ctatcaagca aatgaagaaa ctcctgaagc tgaccaagga cagctccgca gatcgcttcc
     5041 tcagcagcct gggaaggttc atggcagggc tggatacgaa aaacaatgtc aaggtgtggt
     5101 tcaataacaa gggctggcat gctatcagtt cgttcctgaa tgtcatcaac aatgccattc
     5161 tccgggccaa cctgcagaag ggagagaacc cgagccagta tggaatcact gctttcaacc
     5221 accccttgaa cctcactaaa cagcagctct cagaggtggc tctgatgacc acctctgtcg
     5281 acgtcctcgt gtctatctgt gtcatctttg cgatgtcctt tgtccctgcc agctttgttg
     5341 tgttcctgat ccaggagcgt gtgagcaaag ccaagcatct tcagttcatc agcggcgtga
     5401 agcctgtcat ctactggctg tccaattttg tctgggatat gtgcaattat gtggtccctg
     5461 ctacactggt cattatcatc ttcatctgct tccagcagaa gtcctatgtg tcctctacca
     5521 acctgcccgt tctagccctt ctgctcttgc tgtatgggtg gtcgatcact cctctcatgt
     5581 atccagcatc ctttgtgttc aagatcccca gcaccgccta tgtggttctc accagtgtga
     5641 acctcttcat cggcatcaat ggcagtgtgg ccactttcgt actggagctg tttacaaaca
     5701 ataagctcaa tgacatcaat gacatcctga agtctgtgtt tcttatcttc ccacattttt
     5761 gcctggggcg agggctcatc gacatggtga agaaccaggc catggccgat gccctggaga
     5821 ggtttgggga gaaccgcttc gtctctccgc tctcttggga cttggtagga cggaaccttt
     5881 ttgccatggc cgtggaaggg gtggtgttct tcctcattac tgttctgatc cagtacagat
     5941 ttttcatcag gcccagacct gtaaaggcga agcttcctcc tttgaatgac gaggatgagg
     6001 atgtgaggcg ggagagacag aggattctgg acggtggcgg acagaatgac atcctagaga
     6061 tcaaggaact gaccaagatc tataggagga agcggaagcc tgcagtcgac aggatctgca
     6121 tcggcatccc tcccggagag tgctttggac tcctgggagt taacggagct gggaagtcaa
     6181 caactttcaa gatgctgact ggagacaccc ctgtgaccag aggggatgcg ttccttaaca
     6241 aaaacagcat cttatcaaat atccatgaag tacaccagaa catgggctac tgccctcagt
     6301 ttgacgccat cacagagctg ctgacgggaa gagagcatgt ggagttcttt gccctcctga
     6361 ggggagtccc agaaaaggaa gttggcaagg ttggtgaatg ggcaattcgc aaactgggcc
     6421 tggtaaagta tggagaaaaa tatgccagta actacagtgg cggcaacaaa cgaaagctct
     6481 ccacagccat ggctttgatt ggcggacctc ctgtggtgtt tctggatgaa ccaaccacag
     6541 gcatggaccc taaagcccgg agattcttgt ggaattgtgc cctaagcatt gtcaaggagg
     6601 ggagatctgt agtccttaca tctcatagta tggaagaatg tgaagctctt tgtacaagga
     6661 tggccataat ggtcaatgga aggttcaggt gccttggcag tgtccaacat ctgaaaaaca
     6721 ggtttggaga tggttataca atagttgtac gaatagcagg ctccaaccct gacctgaagc
     6781 ctgtccagga gttctttgga cttgcgtttc cgggaagtgt cctaaaagag aaacatcgaa
     6841 acatgcttca gtaccagctt ccatcctcct tgtcatctct agccaggata ttcagcatcc
     6901 tctcccagag caaaaagcga ctccacatag aagactactc tgtctctcag acaacacttg
     6961 accaagtatt tgtgaacttt gccaaggacc aaagtgatga tgaccactta aaggacctgt
     7021 cactgcacaa aaaccagaca gttgtggatg tggccgttct cacatccttt ttgcaggatg
     7081 agaaagtgaa agaaagttat gtatgaagaa tcccgttcac acagggtgaa tgaaaggaag
     7141 gaagagcgag gtcttccttt gcactgtgtc aagtgttcta gaagaaagcg tcctgcgttt
     7201 ctgtggagaa gaacaaactg gatactgtac tgacactatt caatgcaatg cacttcaatg
     7261 caacgagaac acaattccat tacaggggca gtgcctttgt agcctatgtc ttgtatggct
     7321 ctctagtgaa aatgacttga agttagttca ttaccttata cagatgtgaa actctggtgt
     7381 ggaaccaagc agactctggg tttggattca tacttttttt gttcctgtgt attctcacta
     7441 ggattgcaac aacagtctat caaatagtca tggccagtga taatcaaagt caaaggcaca
     7501 cacatcctcg tccattaagc cattaagcca tgctgaacca caaaacaggt ttcccggtga
     7561 cacatccatt gctggcaatg agtgtgccag agttactagt gccaagttgc tcagaaagtc
     7621 tgaagcactg agtgtgtcac aaacactttt gtgaaaaccg ccctactgtc ggttgacatc
     7681 attaaatatc aggtgacaaa aacggtgcca catgtgacta aatccccatt ttccttctct
     7741 ctttgatgag ctgctgttgt ggctgtcttg tacaaaatgt gcatctctct aacccaggta
     7801 cttggttctc ttgttcagtg ttgcccatgc ccctgtcatg gcttgccata gcatctttct
     7861 gagacttttt caaatacatt agatcctcct aagcagcaaa gattagcagc caaactgctg
     7921 gggctgcaag cggctcagtt cagggtgtgg catgaaagat ccagtgcatt tacacgtggc
     7981 aggtctgggt tggtttgtgc tgactgtctc ctaacacaag atgggtacac tacaccttga
     8041 gatccttcat tatttaacag aaatctagtt ctttctaact gtttgaatta acctagaaaa
     8101 tgaaagggtg gcatttcatt ttgacaaaaa tgtttgcatt gttagtatta tttggaattt
     8161 taagttttat caatgcttct ggaagcttag aactgtacac gtgtgatgtc agtcacatag
     8221 aggaatgtgc ccggactgcc tcatgccttt attttccttg gtaaatttga agatagaatg
     8281 tctgactagc gcagtgacca gaaaacaatg tggtagtcaa catctcaggc catattttaa
     8341 gatcctgtag agcactattc atttcaggtt gcagatggag tatttttgaa acatcattac
     8401 tatgtagatg cttggatagg agtgaggggg agctagcaga tttcctgtgc catttattca
     8461 gctgattgat gtacagatgt aggtttattt tgtaaaatcc actgaaagaa tatggccaca
     8521 cccttgccta cttgatagca tcaatacaga agccaagaag gaccactaag taaccccctc
     8581 ttcccaggga gagcagctag cttgaaatct ctcggataca atcgatgcgt ctgacctttg
     8641 ggatcctcac catatgggca aacaatgggc tttgcaggat gagagacacc cacttaaacc
     8701 tctgacgatc tcgaatggtt catctcttcc gtcattaacc agtcatggaa aacaatcaac
     8761 aaactctgcc acgtgaaata ttttttcaga cttttctaac ccaagcttaa tttgttttca
     8821 tctgcaagca acgtggaaaa aaatagttat ttcaccagtg ctcaatgtca tccgtcttca
     8881 tacaaaacaa aaatatgagt aagttcctgt attgggcaaa gttcaagtga gcttttgcta
     8941 tccttattta acttcttcta aaaatttgga agaataagat ttcagatcta tttcaaatta
     9001 tgtttatata atgttatgta attcattgct tgaaaggaaa aatgacaagt ttgatactaa
     9061 tgtcaacata ggtttctaaa atgataagga aagtgtgttt ccagtagtta ttggcatacc
     9121 attcatttta gatatcaaag ataccgaaat caaagaagca tgtctttatt tttcaaaaac
     9181 aaacttccgt tggctttctc agtccattat caccatgtac agcccttaga aaaatccttg
     9241 ttttaattac tcagattttt gaggccacaa aacaacaaca aaagtaaggc accatttttg
     9301 aaaacaaaat caattgagtg tagatttaaa atcttttctg aagctagaga caatctgtag
     9361 ctattatgtt accttttaca ctatctgaag agcaatcttc aactttcctg tttaaacgta
     9421 attattttag aaagttaaaa atcaatccta tcttcaaata agaatttctt agattcccta
     9481 tggagatgtg cattgtgagt tgagaaattc tcaagtgcaa acacttctgg tttactttaa
     9541 aaaaaaatag tggaaaactt actaactgtg aatatgagaa agacaaaaga aagtgagtcc
     9601 tctcgctaga tatacctagc ataattcatt atgaaacaaa caaacctcaa actactgtat
     9661 tttggtgtct gtaccgcaag catatgcctc atgactataa atgttacata catcatttgc
     9721 tctctgtata gtaggcattg acttaaaggg attgtttgtt gtcttctcat ggttgtatat
     9781 atcaggtaaa attgttccaa agagccatgt gttatgtaat ggggaaccac tttgatactg
     9841 aattactaat ttgtactttc attactattt gctggtaata gtgtaacgcc acagtaatac
     9901 tgttctgatt caaaactgtt gcatcccttt ttgtagaaca tttatatttc cataaggatt
     9961 cggtatgttc ttttccctcc tgccctagga tgaagctggt tttgtgcttt ttctttatca
    10021 ttggccctca ttccaagcac tttatgctgt ctgtaatgga tctatttttg cactggaata
    10081 tctgagattt gcaaaactag acaaaagttt cagagcagat ttctaagtta aatcgttttc
    10141 attaaaagaa aaaaaaactg aaaaaaattg tattgtgaat aactttatat gacgtgtttt
    10201 tttaaaagct tgtttctatg ttatgagtca caaaataaag ctgtgacagt cctgctggtc
//
