LOCUS       NM_198608               3368 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus alanyl-tRNA synthetase 2, mitochondrial (Aars2),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_198608 XM_128656
VERSION     NM_198608.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Hilander,T., Zhou,X.L., Konovalova,S., Zhang,F.P., Euro,L.,
            Chilov,D., Poutanen,M., Chihade,J., Wang,E.D. and Tyynismaa,H.
  TITLE     Editing activity for eliminating mischarged tRNAs is essential in
            mammalian mitochondria
  JOURNAL   Nucleic Acids Res 46 (2), 849-860 (2018)
   PUBMED   29228266
  REMARK    GeneRIF: Editing-deficient mitochondrial alanyl-tRNA synthetase
            (mtAlaRS) mice, bearing either a mild or a severe mtAlaRS editing
            mutation, and indicate that editing of mischarged tRNAs by
            alanyl-tRNA synthetase is an essential protein quality control
            mechanism in mammalian mitochondria.
REFERENCE   3  (bases 1 to 3368)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB872387.1 and BC079844.1.
            
            [WARNING] On Jul 24, 2024 this sequence was replaced by
            NM_198608.3.
            
            On or before Sep 27, 2004 this sequence version replaced
            XM_128656.5, NM_198608.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079844.1, AK167771.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-275               BB872387.1         2-276
            276-3368            BC079844.1         248-3340
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.51 cM"
     gene            1..3368
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /note="alanyl-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:224805"
                     /db_xref="MGI:MGI:2681839"
     exon            1..241
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..2956
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /EC_number="6.1.1.7"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; alanyl-tRNA synthetase, mitochondrial;
                     alanine--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="alanine--tRNA ligase, mitochondrial isoform 1
                     precursor"
                     /protein_id="NP_941010.2"
                     /db_xref="CCDS:CCDS28809.1"
                     /db_xref="GeneID:224805"
                     /db_xref="MGI:MGI:2681839"
                     /translation="MAVALAAAAGKLRRAIGRSCPWQPFSTEPGPPHGAAVRDAFLSF
                     FRDRHGHRLVPSATVRPRGDPSLLFVNAGMNQFKPIFLGTVDPRSEMAGFRRVVNSQK
                     CVRAGGRHNDLEDVGRDLSHHTFFEMLGNWAFGGEYFKEEACSMAWELLTQVYGIPED
                     RLWVSYFSGDSQTGLDPDLETRDIWLSLGVPASRVLSFGPQENFWEMGDTGPCGPCTE
                     IHYDLAGGVGSPQLVELWNLVFMQHYREADGSLQLLPQRHVDTGMGLERLVAVLQGKR
                     STYDTDLFSPLLDAIHQSCGAPPYSGRVGAADEGRIDTAYRVVADHIRTLSVCIADGV
                     SPGMSGAPLVLRRILRRAVRYSTEVLQAPPGFLGSLVPVVVETLGSAYPELEKNSVKI
                     ASLVSEDEAAFLASLQRGRRIIDRTVKRLGPSDLFPAEVAWSLSLSGNLGIPLDLVEL
                     MLEEKGVKLDTAGLEQLAQKEAQHRAQQAEADQEDRLCLDVHALEELHRQGIPTTDDS
                     PKYNYTLHPNGDYEFGLCEARVLQLYSETGTAVASVGAGQRCGLLLDRTNFYAEQGGQ
                     ASDRGYLVRTGQQDMLFPVAGAQLCGGFILHEAMAPERLQVGDQVQLYVDKAWRMGCM
                     VKHTATHLLSWALRQTLGPTTEQRGSHLNPERLRFDVATQTLLTTEQLRTVESYVQEV
                     VGQDKPVFMEEVPLAHTARIPGLRSLDEVYPDPVRVVSVGVPVAHALGPASQAAMHTS
                     VELCCGTHLLSTGAVGDLVIIGERQLVKGITRLLAITGEQAQQAREVGQSLSQEVEAA
                     SERLSQGSRDLPEAHRLSKDIGRLTEVAESAVIPQWQRQELQTTLKMLQRRANTAIRK
                     LEKGQATEKSQELLKRHSEGPLIVDTVSAESLSVLVKVVRQLCKQAPSISVLLLSPQP
                     TGSVLCACQVAQDATPTFTAEAWALAVCSHMGGKAWGSRVVAQGTGHTADLEAALGTA
                     RAYALSQL"
     exon            242..433
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            434..579
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            580..747
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            748..892
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1038
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1147
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1186
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1298
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1432
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1577
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1750
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1864
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..2005
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2143
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2253
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2362
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2485
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            2486..2596
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            2597..2680
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            2681..2791
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     exon            2792..3349
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3187..3192
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /note="hexamer: AATAAA"
     polyA_site      3206
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /note="major polyA site"
     regulatory      3312..3317
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /note="hexamer: AATACA"
     polyA_site      3349
                     /gene="Aars2"
                     /gene_synonym="Aarsl; AlaRS; Gm89"
                     /note="major polyA site"
ORIGIN      
        1 gcccggagct aagatggcgg tggcgttggc tgccgcagcc ggtaagctgc ggcgagccat
       61 tgggaggtcg tgcccatggc agcctttctc aaccgagccc ggtccacccc acggagcggc
      121 cgtgcgggac gccttcctga gcttcttccg agatcgccac ggccaccggc tcgtgccctc
      181 cgctaccgtg aggccgcgcg gcgaccccag cctgcttttc gtcaatgcag gcatgaacca
      241 gttcaagccc atcttcctgg gcacagtgga tccacgaagt gagatggcag gcttccgacg
      301 tgtagttaat agccagaagt gtgtcagggc tggaggacgc cataacgacc tggaggatgt
      361 ggggcgtgat ctctctcatc atacgttttt cgagatgctt ggcaactggg ctttcggggg
      421 tgaatatttt aaggaggaag cttgtagcat ggcctgggaa cttctgactc aagtctacgg
      481 gatccctgag gacaggttgt gggtctccta cttcagtggt gactcccaga caggactgga
      541 cccagacctg gagaccagag acatctggct aagcttggga gtgcctgcca gccgtgtgct
      601 ctccttcgga ccacaagaga acttctggga gatgggagac actgggcctt gtgggccttg
      661 tactgagatc cactatgacc tggctggggg cgtgggaagc ccccagctgg tagagctttg
      721 gaatctggtc ttcatgcaac actacagaga ggcagatgga agcctgcagc tgctgccgca
      781 gcgacatgta gacacaggaa tgggcctgga aaggctggtg gccgtcctgc aaggcaaacg
      841 ttccacctac gacaccgacc tcttctctcc actgctcgac gccatacacc agagctgcgg
      901 ggctccccct tactctggcc gggtaggggc agcagatgag ggacggatag acacagcgta
      961 ccgagtggta gccgatcaca tccgcacact cagtgtctgc attgctgacg gtgtctcccc
     1021 ggggatgtca ggtgccccgc tagttctccg tcggattctc cgccgagctg tgcgctattc
     1081 cacagaggtc ttgcaggcac ctcctggctt tctaggcagc ctggtgccag tggtggtaga
     1141 gacactggga tctgcttatc cagaacttga gaagaactca gtcaagatag ccagcctggt
     1201 gtcagaggat gaggcagcct tcctggcctc cctgcagcgg ggccggcgga tcatcgatcg
     1261 caccgtcaag cgcctgggcc cttctgattt gttccctgcc gaagtggcct ggtcattgtc
     1321 gctgtctggg aatctgggga ttcccctgga cctggtggag ctgatgctgg aggagaaggg
     1381 ggtgaagctg gacacggcag gactggagca gctagcccag aaggaggctc agcaccgggc
     1441 ccagcaagca gaggcagatc aggaggaccg attgtgtctt gatgtccacg cactggagga
     1501 gctgcaccgt caaggcatac ccacaactga tgacagcccc aagtataact acactcttca
     1561 ccccaacggg gattatgagt ttggcctctg tgaggcccgg gtgttacagc tgtattcaga
     1621 gactgggaca gccgtggcct ccgtgggagc aggccagcgc tgtggcctcc tattagatag
     1681 aaccaacttc tacgctgaac aagggggtca ggcttcagac cgaggctacc ttgtccgtac
     1741 agggcagcag gacatgttgt tccctgtggc tggggctcag ctctgtgggg gttttatcct
     1801 gcatgaggcc atggctcccg agcgcctaca ggtgggggat caagtgcagc tgtatgtaga
     1861 taaggcctgg cgaatgggat gcatggtgaa gcacacggcc acccacctgc tgagctgggc
     1921 ccttcggcag accctcggac cgaccaccga gcagcggggc tcccatctca accccgagcg
     1981 gctgcgcttt gacgtggcca cccagaccct actaaccaca gagcagctac ggacagtaga
     2041 gagctacgtg caggaagtcg tggggcagga taagcctgtg ttcatggagg aagtgcccct
     2101 ggcacacact gcccgcatcc ccggccttcg ctcactggat gaggtgtacc cagaccccgt
     2161 ccgggtggtg tccgttgggg ttcctgttgc ccacgcactg gggccagcct cccaggctgc
     2221 aatgcacacc tccgtggagc tgtgctgtgg gacgcacctg ctaagtaccg gggccgtggg
     2281 agacttggtg atcattgggg aacggcagct ggtcaagggc atcactcgcc tactggccat
     2341 cactggggag caggcccaac aggcccgaga ggtaggccag agcctgtctc aggaggtgga
     2401 agcagccagc gagcgactaa gtcagggcag ccgagacctg ccggaagctc accggctatc
     2461 taaggacata ggacgtctca ccgaggtagc agagtctgct gtgatacctc agtggcaacg
     2521 gcaggagctg cagaccacac tgaagatgct acagcgccgt gccaacactg ccatccggaa
     2581 actggagaag ggccaggcta cagagaaatc ccaggagctg ttgaaacgac actcggaggg
     2641 gcctctgatt gtggacactg tctctgccga gtccctctca gtgctggtaa aggtcgtgcg
     2701 gcagctgtgc aaacaggccc ctagcatatc cgtgctgctg ctcagccccc agcccactgg
     2761 cagtgtcctg tgtgcctgcc aggtggccca ggatgccacg cccaccttca ctgctgaagc
     2821 ctgggcactg gctgtgtgca gccacatggg aggcaaggcg tggggctcca gagtggtagc
     2881 tcaggggact ggacacacgg ctgacctgga ggccgccctc gggacagccc gagcttacgc
     2941 actcagccag ctctgacgag gaccacccag gacacagagg aacaagaggc aggcaagcat
     3001 ctggagccct catgaaacca cagagctttc ctggaggctg aggcagagga ccagcagccg
     3061 gaggccaggt atctgagcca aagaaggtca cccgggccac caggagactg taagaaacaa
     3121 aagccaactg agaggcagca acctcaacag atgtgaagac ccgggctgct ggagctggga
     3181 gcagctaata aaagtcttcc acaggactgg agacttggct cagtggttaa gagcacttgt
     3241 tgttcataga aaggacccag gttcggttcc cagcatccac atggaggctc cctacctcct
     3301 cacgcactag aaatacatgt ggtacacaat aatacaataa tttggtgtaa aaaaaaaaaa
     3361 aaaaaaaa
//
