LOCUS       NM_146217               3728 bp    mRNA    linear   ROD 20-OCT-2024
DEFINITION  Mus musculus alanyl-tRNA synthetase 1 (Aars1), mRNA.
ACCESSION   NM_146217
VERSION     NM_146217.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Gehlen-Breitbach,S., Schmid,T., Frob,F., Rodrian,G., Weider,M.,
            Wegner,M. and Golz,L.
  TITLE     The Tip60/Ep400 chromatin remodeling complex impacts basic cellular
            functions in cranial neural crest-derived tissue during early
            orofacial development
  JOURNAL   Int J Oral Sci 15 (1), 16 (2023)
   PUBMED   37024457
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Shim,J.A., Jo,Y., Hwang,H., Lee,S.E., Ha,D., Lee,J.H., Kim,J.,
            Song,P., Lee,D. and Hong,C.
  TITLE     Defects in aminoacyl-tRNA synthetase cause partial B and T cell
            immunodeficiency
  JOURNAL   Cell Mol Life Sci 79 (2), 87 (2022)
   PUBMED   35067747
  REMARK    GeneRIF: Defects in aminoacyl-tRNA synthetase cause partial B and T
            cell immunodeficiency.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3728)
  AUTHORS   Zoabi,M., Zhang,L., Li,T.M., Elias,J.E., Carlson,S.M. and Gozani,O.
  TITLE     Methyltransferase-like 21C (METTL21C) methylates alanine tRNA
            synthetase at Lys-943 in muscle tissue
  JOURNAL   J Biol Chem 295 (33), 11822-11832 (2020)
   PUBMED   32611769
  REMARK    GeneRIF: Methyltransferase-like 21C (METTL21C) methylates alanine
            tRNA synthetase at Lys-943 in muscle tissue.
REFERENCE   4  (bases 1 to 3728)
  AUTHORS   Hidalgo San Jose,L., Sunshine,M.J., Dillingham,C.H., Chua,B.A.,
            Kruta,M., Hong,Y., Hatters,D.M. and Signer,R.A.J.
  TITLE     Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell
            Self-Renewal
  JOURNAL   Cell Rep 30 (1), 69-80 (2020)
   PUBMED   31914399
REFERENCE   5  (bases 1 to 3728)
  AUTHORS   Tian,C. and Johnson,K.R.
  TITLE     TBX1 is required for normal stria vascularis and semicircular canal
            development
  JOURNAL   Dev Biol 457 (1), 91-103 (2020)
   PUBMED   31550482
REFERENCE   6  (bases 1 to 3728)
  AUTHORS   Furuya,S., Yoshida,K., Kawakami,Y., Yang,J.H., Sayano,T., Azuma,N.,
            Tanaka,H., Kuhara,S. and Hirabayashi,Y.
  TITLE     Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice:
            changes in gene expression and associated regulatory networks
            resulting from serine deficiency
  JOURNAL   Funct Integr Genomics 8 (3), 235-249 (2008)
   PUBMED   18228065
REFERENCE   7  (bases 1 to 3728)
  AUTHORS   Lee,J.W., Beebe,K., Nangle,L.A., Jang,J., Longo-Guess,C.M.,
            Cook,S.A., Davisson,M.T., Sundberg,J.P., Schimmel,P. and
            Ackerman,S.L.
  TITLE     Editing-defective tRNA synthetase causes protein misfolding and
            neurodegeneration
  JOURNAL   Nature 443 (7107), 50-55 (2006)
   PUBMED   16906134
  REMARK    GeneRIF: the mouse sticky mutation, which causes cerebellar
            Purkinje cell loss and ataxia, is a missense mutation in the
            editing domain of the alanyl-tRNA synthetase gene that compromises
            the proofreading activity of this enzyme during aminoacylation of
            tRNAs
REFERENCE   8  (bases 1 to 3728)
  AUTHORS   Tsukiyama,K., Yamada,Y., Yamada,C., Harada,N., Kawasaki,Y.,
            Ogura,M., Bessho,K., Li,M., Amizuka,N., Sato,M., Udagawa,N.,
            Takahashi,N., Tanaka,K., Oiso,Y. and Seino,Y.
  TITLE     Gastric inhibitory polypeptide as an endogenous factor promoting
            new bone formation after food ingestion
  JOURNAL   Mol Endocrinol 20 (7), 1644-1651 (2006)
   PUBMED   16469773
REFERENCE   9  (bases 1 to 3728)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 3728)
  AUTHORS   Mizoguchi,A., Mizoguchi,E., de Jong,Y.P., Takedatsu,H.,
            Preffer,F.I., Terhorst,C. and Bhan,A.K.
  TITLE     Role of the CD5 molecule on TCR gammadelta T cell-mediated immune
            functions: development of germinal centers and chronic intestinal
            inflammation
  JOURNAL   Int Immunol 15 (1), 97-108 (2003)
   PUBMED   12502730
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM943271.1, AK085725.1, AK150227.1 and AA416313.1.
            
            On Feb 28, 2013 this sequence version replaced NM_146217.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK158139.1, AY223875.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                BM943271.1         1-60
            61-3406             AK085725.1         2-3347
            3407-3711           AK150227.1         3335-3639
            3712-3728           AA416313.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 57.9 cM"
     gene            1..3728
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /note="alanyl-tRNA synthetase 1"
                     /db_xref="GeneID:234734"
                     /db_xref="MGI:MGI:2384560"
     exon            1..422
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            423..587
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     CDS             444..3350
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /EC_number="6.1.1.7"
                     /note="alanyl-tRNA synthetase, cytoplasmic; alaRS; protein
                     lactyltransferase AARS1; protein sticky"
                     /codon_start=1
                     /product="alanine--tRNA ligase, cytoplasmic"
                     /protein_id="NP_666329.2"
                     /db_xref="CCDS:CCDS40478.1"
                     /db_xref="GeneID:234734"
                     /db_xref="MGI:MGI:2384560"
                     /translation="MDATLTAREIRERFINFFRRNEHTYVHSSATIPLDDPTLLFANA
                     GMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLG
                     SWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLG
                     LDEARILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLV
                     FIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFMPYFEAIQKGTGA
                     RPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRY
                     SHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPEMVKDIINEEEVQFLKTLSRGRRI
                     LDRKIQSLGDCKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMNGFEEERRLAQL
                     KSQGKGAGDEDLIMLDIYAIEELRAKGLEATDDSPKYNYQSDSSGSYVFECTVATVLA
                     LRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNA
                     QVRGGYVLHIGTIYGNLKVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGEADQ
                     KGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIVNGMIEAAKPVYTQDCPLAAAKAIQGL
                     RAVFDETYPDPVRVVSIGVPVSELLDDPCGPAGSLTSVEFCGGTHLRNSSHAGAFVIV
                     TEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEAKVKAQTAPNKDVQREIADL
                     GEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQP
                     LVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGL
                     KASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN"
     exon            588..776
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            777..922
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1114
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1259
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1405
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1514
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1665
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1790
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..1935
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2114
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2228
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2435
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2620
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2729
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2843
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            2844..2963
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            2964..3050
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3164
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..3715
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3693..3698
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /note="hexamer: AATAAA"
     polyA_site      3715
                     /gene="Aars1"
                     /gene_synonym="Aars; sti"
                     /note="major polyA site"
ORIGIN      
        1 cgggtcactt ttcatcagag cgctctgatt ggatggtgag gtacggctct ggcaccggtt
       61 gtggcgggtg agtatagctt cttgtgcgca tctgagggag caggtaactg ccggcgagtg
      121 gcggtgggct ccgggcaagg aaggacctgg ggacccggac cctcgggaaa aaaagcaagt
      181 gagccccgag agctctacga gaagagccgg cgcttggagg gggaggggca gctgcagcgg
      241 gctgccgggc ggtcccgccc ctggcctggg tcaggacccg gtctccgcag gagccggatc
      301 tcgagagtag cttttgctat tccctcggag cactgagagt ggtgtggttt ctggtggact
      361 tgcagaggac caagaccttg ggaagatcgc ggttgctctt cagtcgccac ccagagctaa
      421 ggttcccgag gagactttcc gaaatggatg ccactttaac agcacgagag atccgagagc
      481 ggtttataaa tttcttcaga cgaaatgagc acacctatgt ccactcttcc gccaccatcc
      541 ctctggatga ccccactctg ctcttcgcca acgctggcat gaaccagttc aaacccatct
      601 tcctaaacac aatcgaccca tctcacccta tggcgaagct gagcagagct gccaataccc
      661 agaaatgcat ccgagctggg ggcaagcaca acgacctgga tgacgtgggc aaggatgtct
      721 accaccacac tttctttgag atgctgggct cctggtcttt tggggactat ttcaaggaat
      781 tggcatgtaa gatggccctg gaacttctta cccaggagtt tggtattcca gttgaaagac
      841 tctatgtcac ttactttggt ggtgatgagg cggccggcct agagccagat ctggagtgca
      901 gacagatttg gcaaaattta ggactggatg aagccagaat cctccctggc aacatgaagg
      961 acaacttctg ggagatgggt gacacaggcc cctgtggtcc ctgcagcgag atccactatg
     1021 accgcattgg tggcagggac gccgcacacc ttgtcaacca ggatgacccc aatgtgctgg
     1081 agatctggaa cctcgtgttc atccagtata acagggagtc ggatggtgtt ctgaaacctc
     1141 ttcccaagaa aagcatcgac acagggatgg gcctagagag gctggtgtct gtgctgcaga
     1201 acaagatgtc caactacgac accgaccttt tcatgcctta cttcgaagcc attcagaagg
     1261 gcaccggcgc ccggccgtat actgggaagg ttggtgctga ggatgcagac ggaatcgaca
     1321 tggcctaccg ggttctggct gaccacgccc ggaccatcac tgtggcgctg gctgatggcg
     1381 gccggcctga caacacagga cgggggtacg tgctgagacg gatccttcgc cgagctgttc
     1441 ggtattccca tgagaaactg aatgccagca ggggtttctt cgctacattg gttgatgttg
     1501 tcgttcaatc cctgggagac gcatttcctg agctgaagaa ggacccagag atggtgaagg
     1561 acatcattaa tgaagaagag gtgcagtttc tcaagactct cagccgaggg cggcgcatcc
     1621 tggaccgaaa aattcagagc ttaggagact gcaaaaccat cccaggtgac accgcttggc
     1681 tcctctatga tacttatgga ttcccagtgg acctcactgg attgattgct gaagagaagg
     1741 gcctggtggt agatatgaat ggctttgagg aagagaggag actggcccag ctgaagtcac
     1801 agggcaaggg agcaggggac gaagacctca tcatgctgga catttacgct attgaagagc
     1861 tccgggcaaa gggtttggag gccacagatg attctccaaa gtataactac caatcagact
     1921 ccagtggtag ctacgtgttt gagtgtacag tggctaccgt gcttgctctg cgtcgggaaa
     1981 agatgtttgt ggacgaggtg gtcactggcc aggagtgtgg agtggtgctg gacaagacct
     2041 gtttctatgc ggagcaagga gggcagatct acgatgaagg ctacttggtg aaggtcgacg
     2101 acagcagtga ggacaaaacc gagtttacag tgaagaacgc tcaggtccga ggcgggtacg
     2161 tgctgcacat aggaacgata tatggtaacc tgaaagtggg ggatcaagtc cggctgttta
     2221 ttgatgagcc ccggcggaga cccgtcatga gcaaccacac agctacacac atcctcaact
     2281 tcgccttgcg ctcggtgctt ggggaagcgg accagaaagg ttcactggtt gctcctgacc
     2341 gccttcggtt tgacttcact gccaagggag ccatgtccac ccagcaaatc aagaaggctg
     2401 aagagattgt caatggcatg attgaggcag ctaagcctgt ctacacccag gattgtcccc
     2461 tggcagcagc aaaagccatc cagggccttc gagctgtgtt tgatgagacc tatcctgacc
     2521 ccgttcgagt cgtctccatt ggggtccccg tgtcagagct gctggatgac ccgtgtgggc
     2581 ctgctggctc ccttacttct gttgagttct gtggaggaac acatctacgg aattctagcc
     2641 acgcaggcgc ttttgtgata gtgacagaag aagctattgc caagggtatc cggaggattg
     2701 ttgctgtcac aggtgctgaa gcccagaagg ctctcaggaa gtcagaaacc ttgaagaaat
     2761 ctctctctgc catggaggcc aaagtgaagg cccagactgc acccaacaag gacgtgcaga
     2821 gggaaattgc tgacctcggt gaggccctgg ccactgcagt catcccccag tggcagaaag
     2881 atgaacaacg agagactctg aagtccctga agaaagtcat ggatgaccta gaccgggcca
     2941 gcaaagccga tgtgcagaag cgagtgttag agaagacaaa gcagctgatt gacagcaacc
     3001 ccaaccagcc gctggtcatc ctggagatgg agagtggagc atcggccaag gccctgaatg
     3061 aagcgttgaa gcttttcaag acgcattctc ctcagacatc tgccatgctg ttcacggtgg
     3121 acaatgaggc tggcaagatc acgtgcttgt gccaggttcc ccagaatgca gccaaccggg
     3181 gactgaaagc cagcgagtgg gtccagcagg tttcaggcct gatggatggt aaaggcggtg
     3241 gcaaggacat gtccgcccag gccacaggca agaacgtggg ctgccttcag gaagcactgc
     3301 agctggctac ctcctttgcc cagctccgtc tgggagatgt gaagaactga tgggagggaa
     3361 gccctctcgg gaaagcaact cgccatcaac cagacatgtc cgaaggcggg aattcagcca
     3421 ggagctctcc tcccgccacg aagacattgg agtcatggga cctctcagca gccctgtccg
     3481 tcctgatcca gcggtaatgg gaacacgcct ggagctgtcc tgtgattcat tgcccccctc
     3541 cattactgct ctgagcccat tcatgtcagc accatctgtc taaaatcact acccaggaat
     3601 gctttttaac gtcatgatca catctagatg aggtcactct gccagcctga ggctttaggt
     3661 ctgcagagga aggaagcctt ttctctgcag aaaataaaaa ggaccatgtg caataaaaaa
     3721 aaaaaaaa
//
