LOCUS       NM_175503               1360 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus alanine and arginine rich domain containing protein
            (Aard), mRNA.
ACCESSION   NM_175503
VERSION     NM_175503.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Geng,Q., Ni,L.W., Ouyang,B., Hu,Y.H., Zhao,Y. and Guo,J.
  TITLE     Alanine and arginine rich domain containing protein, Aard, is
            directly regulated by androgen receptor in mouse Sertoli cells
  JOURNAL   Mol Med Rep 15 (1), 352-358 (2017)
   PUBMED   27959439
  REMARK    GeneRIF: The results of the present study indicate that Aard is a
            target of AR action in mouse Sertoli cells and suggest a novel
            finding by which the loss of AR function in Sertoli cells blocks
            spermatogenesis and results in male infertility.
REFERENCE   3  (bases 1 to 1360)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 1360)
  AUTHORS   West,D.B., Engelhard,E.K., Adkisson,M., Nava,A.J., Kirov,J.V.,
            Cipollone,A., Willis,B., Rapp,J., de Jong,P.J. and Lloyd,K.C.
  TITLE     Transcriptome Analysis of Targeted Mouse Mutations Reveals the
            Topography of Local Changes in Gene Expression
  JOURNAL   PLoS Genet 12 (2), e1005691 (2016)
   PUBMED   26839965
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1360)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1360)
  AUTHORS   Lin,Q., Donahue,S.L., Moore-Jarrett,T., Cao,S., Osipovich,A.B. and
            Ruley,H.E.
  TITLE     Mutagenesis of diploid mammalian genes by gene entrapment
  JOURNAL   Nucleic Acids Res 34 (20), e139 (2006)
   PUBMED   17062627
REFERENCE   7  (bases 1 to 1360)
  AUTHORS   Bouma,G.J., Albrecht,K.H., Washburn,L.L., Recknagel,A.K.,
            Churchill,G.A. and Eicher,E.M.
  TITLE     Gonadal sex reversal in mutant Dax1 XY mice: a failure to
            upregulate Sox9 in pre-Sertoli cells
  JOURNAL   Development 132 (13), 3045-3054 (2005)
   PUBMED   15944188
REFERENCE   8  (bases 1 to 1360)
  AUTHORS   Bouma,G.J., Hart,G.T., Washburn,L.L., Recknagel,A.K. and
            Eicher,E.M.
  TITLE     Using real time RT-PCR analysis to determine multiple gene
            expression patterns during XX and XY mouse fetal gonad development
  JOURNAL   Gene Expr Patterns 5 (1), 141-149 (2004)
   PUBMED   15533830
REFERENCE   9  (bases 1 to 1360)
  AUTHORS   Menke,D.B., Koubova,J. and Page,D.C.
  TITLE     Sexual differentiation of germ cells in XX mouse gonads occurs in
            an anterior-to-posterior wave
  JOURNAL   Dev Biol 262 (2), 303-312 (2003)
   PUBMED   14550793
REFERENCE   10 (bases 1 to 1360)
  AUTHORS   Menke,D.B. and Page,D.C.
  TITLE     Sexually dimorphic gene expression in the developing mouse gonad
  JOURNAL   Gene Expr Patterns 2 (3-4), 359-367 (2002)
   PUBMED   12617826
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK046414.1 and AY134665.1.
            
            On Nov 19, 2006 this sequence version replaced NM_175503.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY134665.1, AK046414.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-61                AK046414.1         2-62
            62-1360             AY134665.1         1-1299
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 19.55 cM"
     gene            1..1360
                     /gene="Aard"
                     /gene_synonym="A5D3"
                     /note="alanine and arginine rich domain containing
                     protein"
                     /db_xref="GeneID:239435"
                     /db_xref="MGI:MGI:2181621"
     exon            1..424
                     /gene="Aard"
                     /gene_synonym="A5D3"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..580
                     /gene="Aard"
                     /gene_synonym="A5D3"
                     /codon_start=1
                     /product="alanine and arginine-rich domain-containing
                     protein"
                     /protein_id="NP_780712.2"
                     /db_xref="CCDS:CCDS49603.1"
                     /db_xref="GeneID:239435"
                     /db_xref="MGI:MGI:2181621"
                     /translation="MGLGDYSHCRQRMSRGLYGVSGRAALWSPVFHPVHRMPCGTWRI
                     GIEVPEHVRASSPVLEHLRRQLERAFQRAAARGRARRAREAVAAVAAAAAAAREERSR
                     TRMECALARLRAELLELRFQNHQLARTLLDLNMKMQQLKKRQDQELASKPQSPQDKEM
                     NSECGSA"
     exon            425..1348
                     /gene="Aard"
                     /gene_synonym="A5D3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1326..1331
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aard"
                     /gene_synonym="A5D3"
                     /note="hexamer: AATAAA"
     polyA_site      1348
                     /gene="Aard"
                     /gene_synonym="A5D3"
                     /note="major polyA site"
ORIGIN      
        1 ccccgacccg atcggcctta aaaagctcca acttgcaacc aaatccccag tcggagcgct
       61 gcagaaaagg accacgatgg gcttgggtga ctacagccac tgcagacaga ggatgtcccg
      121 gggactctac ggagtctctg gaagggctgc gctatggtcc ccagtcttcc acccggtgca
      181 ccgcatgcct tgcggcacct ggcgcatcgg catcgaagtg ccagagcacg tccgggcgtc
      241 cagcccggtg ctggagcacc tccgcaggca gctggagcgc gcctttcagc gggcggcggc
      301 acgcgggcgc gccagacgcg cgcgggaggc ggtggcggcg gtggcggcgg ccgcagcggc
      361 agcacgggag gaaaggagcc ggacgcgcat ggagtgcgcc ctggcccggc tgcgcgcgga
      421 gctgctggaa ctgcgtttcc agaaccacca gctggcccga actttactgg atttaaacat
      481 gaaaatgcag cagctgaaga agaggcagga ccaggagcta gcttccaaac cccagagccc
      541 gcaggataag gagatgaatt ccgagtgtgg aagcgcatag tggagagtcc gaggccacca
      601 ctccaaagca accctgagcc ctcgccgtaa cagggcgctc tgaaaccctc ctagcattaa
      661 cactagcgat gcttatgtgt gctctaaagg acaatttgtc cagcgaaaac ttaggcttaa
      721 ggaagaagtg gggaaaaaag ctaagaaagg gggcgagact tttttgtttg tttgttttaa
      781 aaatgttcat tcattggttt gactggttat tgtcttatta gtcgaaggga ggaggggtga
      841 ggaggatgga ggtggaagac aaccaaagca ctaaaagttt gccttaatgc cagcttacaa
      901 cgctacacgt taccttcatg tagcagtgca tgaacttgca gaatctgtgc tcatttttac
      961 tttatttatt tttggctgat ctagtagcct tcccagtcct cttctgattt gtgaagctga
     1021 gaattacttg tgtctagatt ccacccatac cctcgatgtg gccagcgaga agggcttctc
     1081 acctttttgc tgaatgtgga catgtatttc aggacttgct gtcattgaaa tggaaaacac
     1141 taactattct tctcataaaa tttcttgctt cagttgattc aagtttgatt tttcaagatg
     1201 cacaccccta gattcctgac taatttagtt cttttgttca gttaatttgg gagtttaagt
     1261 ggttcttatt gaattaaaag gttgaattgt acacccaaag tgctttacaa tgcaaaaata
     1321 aacttaataa aaatagttgc agacctaaaa aaaaaaaaaa
//
