LOCUS       NM_001378654           10196 bp    mRNA    linear   ROD 19-NOV-2024
DEFINITION  Mus musculus AP2 associated kinase 1 (Aak1), transcript variant 4,
            mRNA.
ACCESSION   NM_001378654
VERSION     NM_001378654.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   Taoufiq,Z., Ninov,M., Villar-Briones,A., Wang,H.Y., Sasaki,T.,
            Roy,M.C., Beauchain,F., Mori,Y., Yoshida,T., Takamori,S., Jahn,R.
            and Takahashi,T.
  TITLE     Hidden proteome of synaptic vesicles in the mammalian brain
  JOURNAL   Proc Natl Acad Sci U S A 117 (52), 33586-33596 (2020)
   PUBMED   33376223
REFERENCE   3  (bases 1 to 10196)
  AUTHORS   Fu,X., Ke,M., Yu,W., Wang,X., Xiao,Q., Gu,M. and Lu,Y.
  TITLE     Periodic Variation of AAK1 in an Abeta1-42-Induced Mouse Model of
            Alzheimer's Disease
  JOURNAL   J Mol Neurosci 65 (2), 179-189 (2018)
   PUBMED   29774516
  REMARK    GeneRIF: Periodic variation in AAK1 expression is closely
            correlated to the decline in cognition in mouse model of
            Alzheimer's disease.
REFERENCE   4  (bases 1 to 10196)
  AUTHORS   Hu,W., Li,S., Park,J.Y., Boppana,S., Ni,T., Li,M., Zhu,J., Tian,B.,
            Xie,Z. and Xiang,M.
  TITLE     Dynamic landscape of alternative polyadenylation during retinal
            development
  JOURNAL   Cell Mol Life Sci 74 (9), 1721-1739 (2017)
   PUBMED   27990575
REFERENCE   5  (bases 1 to 10196)
  AUTHORS   Munton,R.P., Tweedie-Cullen,R., Livingstone-Zatchej,M.,
            Weinandy,F., Waidelich,M., Longo,D., Gehrig,P., Potthast,F.,
            Rutishauser,D., Gerrits,B., Panse,C., Schlapbach,R. and Mansuy,I.M.
  TITLE     Qualitative and quantitative analyses of protein phosphorylation in
            naive and stimulated mouse synaptosomal preparations
  JOURNAL   Mol Cell Proteomics 6 (2), 283-293 (2007)
   PUBMED   17114649
REFERENCE   6  (bases 1 to 10196)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   7  (bases 1 to 10196)
  AUTHORS   Jha,A., Agostinelli,N.R., Mishra,S.K., Keyel,P.A., Hawryluk,M.J.
            and Traub,L.M.
  TITLE     A novel AP-2 adaptor interaction motif initially identified in the
            long-splice isoform of synaptojanin 1, SJ170
  JOURNAL   J Biol Chem 279 (3), 2281-2290 (2004)
   PUBMED   14565955
REFERENCE   8  (bases 1 to 10196)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 10196)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157099.5 and AC159712.7.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.776643.1,
                                           SRR7345562.3703620.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-711               AC157099.5         75076-75786         c
            712-830             AC157099.5         159-277             c
            831-939             AC159712.7         83506-83614
            940-1082            AC159712.7         85018-85160
            1083-1204           AC159712.7         92433-92554
            1205-1286           AC159712.7         94180-94261
            1287-1419           AC159712.7         94937-95069
            1420-1523           AC159712.7         97618-97721
            1524-1603           AC159712.7         98857-98936
            1604-1758           AC159712.7         103180-103334
            1759-2072           AC159712.7         104335-104648
            2073-2315           AC159712.7         107108-107350
            2316-2545           AC159712.7         112072-112301
            2546-2703           AC159712.7         113553-113710
            2704-2808           AC159712.7         115345-115449
            2809-10196          AC159712.7         143916-151303
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 37.75 cM"
     gene            1..10196
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /note="AP2 associated kinase 1"
                     /db_xref="GeneID:269774"
                     /db_xref="MGI:MGI:1098687"
     exon            1..711
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     CDS             549..3932
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /EC_number="2.7.11.1"
                     /note="isoform 3 is encoded by transcript variant 4;
                     adaptor-associated kinase 1; AP2-associated protein kinase
                     1"
                     /codon_start=1
                     /product="AP2-associated protein kinase 1 isoform 3"
                     /protein_id="NP_001365583.1"
                     /db_xref="GeneID:269774"
                     /db_xref="MGI:MGI:1098687"
                     /translation="MKKFFDSRREQGSSGLGSGSSGGGGSSSGLGSGYIGRVFGIGRQ
                     QVTVDEVLAEGGFALVFLVRTSNGVKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKN
                     IVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCE
                     AVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQAEGVNAVEDEIK
                     KYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGSFTI
                     PDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPVPNVQNSPIPAKLP
                     EPVKASEAAVKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRK
                     RATVQPLPQAAGPSNQPGLLPSVSQPKAQATPSQPLQSSQPKQPQAPPTPQQTPATQT
                     QGLPTQAQATPQHQQQHLLKQQQQQQQQPQQPTAPPQPAGTFYQQQQQQQQQQAQTQQ
                     FQAVHPAAQQPVTAQFPVGSQGGAQQQLMQNFYHQQQQQQQQQQQLMAQQAALQQKTA
                     VVVPQSQAQPATAPQAAAAQEPGIQAPVRQQPKVQTTPPPTIQGQKVGSLTPPSSPKT
                     QRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQN
                     VSNASEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKLPEKLGGSAESLIPGFQPTQ
                     GDAFTTPSFSAGTGLQRDPSPCPVITVEHFKESMGTKGLPLYPDPCRAPGTKTQNTLA
                     SDSLAREGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLESEEEDENCRPLQEKLGEA
                     ALFSESGVCTEPEERGQGGKKSQFLPINQRASDDLGEPDVFATAPFRSSLVPADDVDI
                     FSKAPFVSKGSVAPSQMDEVDVFSRAPFTKKRSMEEFLAVQGSSQDLPMQANLSQSNE
                     GPLLAGRDRAIYTPAQAQYPMTGFAPQAGLPSHSVQVADHFDGNSPRGSPMSSGGHPV
                     DRNRGLQPQKEAFSGPAAGKPFHPQALSKYSRHYSPEDELSAEAQPIAAYKIVSQSNK
                     QLLAGSVSVTSLSSRTTELATADPFALAPFPSKAGKQKP"
     exon            712..830
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            831..939
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1082
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1204
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1286
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1419
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..1523
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1603
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1758
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..2072
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2315
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2545
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2703
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2808
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     exon            2809..10196
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10173..10178
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /note="hexamer: ATTAAA"
     polyA_site      10196
                     /gene="Aak1"
                     /gene_synonym="5530400K14Rik; 9630042K20; D6Ertd245e;
                     Gm38495; mKIAA1048"
                     /note="major polyA site"
ORIGIN      
        1 agcgcggcgc tcggggatca gctggcgggc gggcgggcgc cgaagcggcc ccggctctgc
       61 ggcagcgccg cctccttcgt gtcgcaggcc ggcggccgtg gccatgtcgc ggggctggta
      121 gctgggctcg ccgccgagcg gccgctagtg ggtgtccggg gcccggggcg ggggtccggg
      181 caggggtccg gtgcgtttcc agggctttcc cgaagaaaat gcacgcagcg tttcctctgg
      241 atgacggcct gtgctttccc taggacccgg cagttaggct ctggagtgtg ttactgattc
      301 atcctctctc tgcctctgtc atcgcaggtt taaacttaaa cgatcgctcc ccgaggagga
      361 ggggacccgc cgcgcgattg acagtctatt cctgcaggcg ccgtccctca gcccccaccc
      421 cacggccgga ttcccggggc tctgctcagg gctgaaaccg gagaagaaat ccttagatct
      481 cttttcttta caacaaatac gaacctagaa accatcggca ttttgccttg gcatttcata
      541 ttggaaagat gaagaagttt ttcgactcca ggcgggagca ggggagctct ggcctgggtt
      601 caggctccag cggaggaggg ggcagcagct caggcctggg cagtggttac atcggaaggg
      661 tcttcggcat cgggaggcag caggtcaccg tggacgaggt gttggcggaa ggtggattcg
      721 ctcttgtctt tctggtgaga acaagcaatg gggtgaaatg tgccttgaaa cgtatgtttg
      781 tcaacaatga acatgacctc caggtgtgca agagggagat ccagatcatg agagacctat
      841 cagggcacaa gaacattgtg ggctacattg attctagtat caacaatgtg agcagcggcg
      901 acgtctggga agttctcatt ctcatggact tctgcagagg aggccaggtg gtaaacctga
      961 tgaaccagcg tctccagaca ggcttcacag agaatgaagt gctgcagata ttctgtgaca
     1021 cctgtgaagc tgtggcccgc ctgcatcagt gcaaaactcc cattatccac cgggacctga
     1081 aggttgaaaa tatcctcttg catgaccgag gccactatgt cttgtgtgac tttggaagtg
     1141 ccaccaacaa attccagaat ccacaggccg agggagtcaa tgcagtagaa gatgagatta
     1201 agaaatacac aacgctctcc tatcgagccc cagaaatggt caacttgtac agtggcaaaa
     1261 tcatcactac gaaggcagat atttgggctc taggctgttt gttgtataaa ttatgctact
     1321 tcactttgcc gtttggggag agccaggtgg cgatttgtga cggaagcttc acaattcctg
     1381 ataactctcg ttattctcaa gatatgcact gccttattag gtatatgttg gaaccagacc
     1441 ctgacaaaag gccggatatt taccaggttt catacttctc atttaaacta ctcaagaaag
     1501 aatgccccgt tccaaatgta cagaactctc ccattcctgc aaaacttcct gaaccagtga
     1561 aagccagtga agcagctgtg aaaaagaccc agccaaaggc cagactgacc gatcccattc
     1621 ccaccacaga gacttcaatc gcaccccgcc agaggcctaa agctgggcag actcagccaa
     1681 acccaggcat ccttcccatc cagccagccc tgactcctcg gaagagggcc actgttcagc
     1741 ccctacctca ggctgcagga cccagcaatc agcctggact tctacccagt gtttcccaac
     1801 ctaaagccca agccacaccc agtcaacctc ttcagtcatc tcaacccaag caaccacaag
     1861 ctccacccac cccacagcag acacctgcta cccagacaca aggtttgccc acccaggccc
     1921 aggccactcc ccagcaccag cagcaacacc tcctcaagca acagcagcaa cagcagcaac
     1981 agcctcagca gcctacagca ccaccacagc ctgcaggcac cttttaccag cagcagcagc
     2041 aacagcagca gcagcaggct cagactcagc agtttcaggc agtacatcca gcagcccagc
     2101 aaccagtcac tgctcagttc cctgtggggt cccagggagg tgctcagcaa cagctgatgc
     2161 agaacttcta ccaccagcag caacagcagc agcagcagca gcagcagctg atggctcagc
     2221 aggctgccct gcagcagaag actgctgtgg tagtaccaca gtctcaggca cagcccgcca
     2281 cagccccaca ggcagctgct gcccaggagc ctgggattca agccccagtg agacaacagc
     2341 caaaagttca gaccactcca cctccaacca tccagggaca gaaagttgga tctctcactc
     2401 ctccatcatc acccaaaacc caacgtgctg ggcacagacg gattctcagt gatgtaaccc
     2461 acagtgcagt ctttggggtt cctgccagca aatcaaccca gctgctccag gcagctgcag
     2521 ctgaggccag tctcaataaa tctaagtctg caaccaccac tccctcaggc tctccacgga
     2581 cttcacagca gaatgtctcc aatgcttcag aaggctccac atggaatcct tttgatgacg
     2641 acaacttctc caaactcaca gctgaagagc tgctaaacaa ggactttgcc aagcttgggg
     2701 aaggaaagct gcctgagaag cttggtggct ctgcagagag tttgatccca ggcttccagc
     2761 caacacaagg agatgccttc accactcctt ctttttctgc tggaactggt ctccagagag
     2821 atcccagtcc ttgtcctgta attactgtag agcactttaa agaatccatg ggcaccaaag
     2881 gccttcccct ctacccagat ccctgcagag ctcctggcac aaagactcag aacaccttag
     2941 catctgattc tctggcccga gagggtcctt ccagcaacag ctccttccac agcagtgagg
     3001 aggaggggac tgacctggag ggggacatgc tggactgcag tgggtctcgg cctctcctgg
     3061 agtctgaaga ggaggacgag aactgccggc ctctccagga gaagctggga gaagccgccc
     3121 tgttctctga atctggagtg tgcactgagc cagaagaaag gggacaaggc gggaaaaaga
     3181 gccagttctt gccaatcaac cagcgagcct cagacgatct tggggagcct gatgtctttg
     3241 ccacagcccc cttcaggagc tctctggttc cagcagacga cgtggacatt ttctccaaag
     3301 ccccttttgt ttccaagggc agcgtagcgc cttcccagat ggatgaagta gatgtgttct
     3361 ccagagctcc ttttaccaag aagaggagca tggaggagtt cctggctgtg cagggctcct
     3421 cacaggattt gccgatgcag gcaaacctca gtcagtccaa cgaagggccc ctacttgcag
     3481 gccgagatag agccatttac acccctgcac aggcgcagta tcctatgact ggctttgcac
     3541 cgcaggctgg tctcccctcc cactctgtcc aggtggcaga ccattttgat ggcaactctc
     3601 ccagggggtc tcctatgagc tctgggggcc atcctgttga cagaaacaga ggactgcagc
     3661 cccagaaaga agccttctca ggcccagcag ctggcaagcc attccacccc caggctttat
     3721 ccaaatattc tcgacactat agcccagaag atgagctaag tgcagaagcc cagcccattg
     3781 ctgcctacaa gattgtttca caaagcaata agcagctgct agctggctct gtgtctgtca
     3841 catccctgtc ctccaggact acagagctgg ccactgctga tccttttgct ttagccccct
     3901 ttccttcaaa agcaggcaaa cagaaaccct aggagcagag acgagcctga ggcctgcctc
     3961 cacccacccc accagcaccc accacaccac tcctggctgg cctttcaagg agcaaatccc
     4021 acccactgtg accatgcagt tccctgcctc agagcagaat cacttcaagg caactagcca
     4081 agttgccacg agacctggca gaagctcctt atgtcacatg tactgctttg gtttatattc
     4141 atacccaggc ttgtagatgg ttgtgtcacc ttcatagagg ccttccaccg cgctcagaac
     4201 tctgattttt cttttttttc tgcccagaaa cctttttaat ccaggaatat tttaccccta
     4261 gttaacaggg gctatattat accatgactt tgaattccta cagctcctgt tggagtcact
     4321 tgtattgaag aattaagatt gatccctcca ggactcatca atactcttgg aacagagaag
     4381 tttctagaaa gaagagcaat acaaacctac tgcacatctg aagaagttaa tcttttagct
     4441 actcatcatt cattcctatg gagccagcac ctcgcaaagc actctgggat ctgtcaccaa
     4501 gtctgtagga gccaagctgg ccctgtgcac aagtgtctgg gtgcatactg tgggtcagac
     4561 tctgtgtcga gtaaaggaac acacagatgg gagcagcatt ctgcctccca cggtctacat
     4621 ttagactagt gtggacgctt gtatgctcat gcatatgaga aactcttgtc ctttgagtga
     4681 aaccaccatg cctacctcca ttagcgaatc actcgttttt cttgcatttg cctgttgacc
     4741 tgacttgaac cttggcttta ttagattttt tttttttctt ctcatggtag aattgaagtt
     4801 gttggacaat ttggggcaat cctgctggaa ctgactgagg tgtcagagtg aattttttaa
     4861 attccattta cctggttttg aaaaaatcaa tttcctaaaa ctggacatgg tagataccta
     4921 atgaatgtaa tcctagcact tgggaagctg aggcaggagg attgccacaa gttaagagat
     4981 tagtctgggc ttaatactga attccaggtc tctatatctc aacaccttct ccttccagcc
     5041 aaaaaagtca tcagtctttg tgtttccctg gcaacagggg aagagaatct tcagaggtcc
     5101 agccacgggg actcctgcct gggtaccatt gttgtgaaat tcattaatca ggagcccaag
     5161 ccacaagcta agtttgcctg ttatttctcc caaacccttc ccatacacct ctactgcctc
     5221 ttaagtgggt acagacctga gacccggaag taaactgaga atggaaaaga gttggtcagt
     5281 tgtccttgca aatctttaaa tgatctgtgc ctttcagatg ttccagtaga gcagacaggt
     5341 gactggctgc gtgttcagga gtgtgttttt atatctggac ggctctgacc attcctagtg
     5401 gttttcatgt cctttatttt tactgcctta gaataaaccc tgactccatg aggcatccac
     5461 tgaggaaaga gttgcagcaa acccaggtgt gcactcatgg cttctcacag acactgcttc
     5521 cacttgcctg cggaagtgtg gagagctgtg tgttgcgctg gattagtgat ggaagaaagg
     5581 ctagaagcta ggcacagtag cgtaccgctg caaacagggc actcgggtgg tacagaaaga
     5641 aggaattcca gggcagccta gactgcacga cacactgtcc caaaagagaa aatccatcca
     5701 gggctaaagc ctgaatgcca ggctgcactt gcaatcactt agttgtgata gccttaaaat
     5761 ttgaaagtca gggtttgggt gcacaaggca gcagcagaag atatgcttag cttgcacaat
     5821 gccctggggt ctgtcgtcag tacacaaaaa caatacagtt aaatctagaa gtcttgacgg
     5881 taatataaga atgactattt ttttatgata tcaggtactt ttaaccccag aacttgcgag
     5941 gcaaggacag gtggatctct gtgagtttga agccagcctg tctacataac aagttccagt
     6001 ccagccagtg agtgaaaccc tgtctttaga ggggggtagg gggctatttt tccctctttt
     6061 cttcatagca ttctccacct gtttcaataa caggcactgc gtcggggccc atagcagcgc
     6121 catttgagga ctccacccca ccagaaacct gcctttcact tcagtaaagg ttaaacattt
     6181 tctttaggaa ttttctttct cacaattatt attttttaat ttcctgtata atagtggata
     6241 caaaattgtc tttctattaa ataagtagtt tgactaacgc acatggaact ttgcaaccaa
     6301 acttcccaag cttgccgtca gatgaaggcc tccaaggcac ttgcagaaag gatggtgatg
     6361 ttcagggagc ccttgcttaa tgtcatagct cctcccaggt tgcagcacat acctgtagtc
     6421 ccagctgctt aggaggctgg ggcagaaaga ctgcttgagc ctgggatctc atggccagcc
     6481 ttggcaacac agaaggaccc atctcttgat gagaaacttc aaaatgcaag gtcaacacca
     6541 cctcggtaat agctctgcaa actattcttc ctttgattac tggattgctc catctaatca
     6601 gtcatttttt tctcacaagg tccaaattag aataaaatgg attctttatc ttatatagtt
     6661 taatcatttg tgaagtgtgg gcttagggcc tctttttgta ctgagtggaa agtctatcta
     6721 ccatcttaga atagacagtg aaaatgacca catgcagact gtttttctta catagtgatt
     6781 cgtgaaggaa agctgacaca tgataacccg tgatgagttc tgaaggaggc gctgggatga
     6841 tcctggatta caggatcagt gtgtaatgta aagattaggt atctttgtgg gatgccatcc
     6901 tgaatggaaa tccagagatg tttgtcttac agggaattat gagtgcttaa cgtctgtgga
     6961 tcaggagcag gttccctggg accatactgt cacaacttaa taataaggaa gaggagaaca
     7021 ttgtcaacaa tgtactgcac tctctttgtt ttagtgtctg ctgtgtgttt gtcactttgt
     7081 atatttcata ggaaacacca gagattctgg acttttgact cagtagacag aaatgagcca
     7141 gagtttcctt aggcaacgcc ttatgctgta tcatatacta atgaactcct aaatgatgtg
     7201 ctaggctggg agcatggctc agagatggag ccggtggtgc acaagagagg ccctaggttc
     7261 agccctatta actgactaat gagcactgtt tacagagtta acttaagtgt gaccaatttc
     7321 ttaggcttac agtacagttc tttagcacca aaaccttggt tggagtcaaa acaaatgaaa
     7381 tgaaataatg aagtgagcct ctgtggcatg ccaaggcaaa cggattgcga attcaaattt
     7441 attctagcac aggcagctta gcaagctctt actatcgaac tttaaagcag cttggtttat
     7501 agtcaggtgg gagagcactt agttagcacg tgtgaccact gggttttata aaactgaagt
     7561 cccgggaaac agtgcaagat gcttccatca tgtgatggtg atcagtctca ccagagaaag
     7621 tgagcagaag ttggtgctca aaaggaaatt tcagttaata cagcctgttt tgtcctggct
     7681 tatttgtgga gttgaagacc tatgtactgg cacatgccct agtgtgtgtg catatgtgtg
     7741 cgtgtgtgtg cgtgtgcgtg tgtgtgtgtg tgtgtgtgtg tgtgtaacag tcttctactc
     7801 actaacccat ggtatgtctc agtttcatct ggcaactgag ttcttctaaa tggttaagaa
     7861 aatgctgcct tagagcctgt tgtatattta tgtgttggag tctgtgctta tcagtatttt
     7921 cagctagtct tcattgtgcg tgaaatatta gagtggtctg atgataagaa attctgtatt
     7981 tttattctgt aaattagaat aatcaaaaaa tgtatcaaat tgtatgatat atgcattact
     8041 tcaaatagga aatgtaaagt ggaaaaccag ggaccaggag ctgcagtgcc ctccccagca
     8101 gtgagtggac aacttccagt ggagtccatt tgggtccctc agatgtagag atggtgccct
     8161 ctgtggtgtc tttggcattc aaactttaga acacatccca gtcaactcca aaatggctgc
     8221 tagtcacagc atttctctca gcatcgaagt cgttatggtg aaaggtagtc tgcaggagtg
     8281 cagtggtaga ggcaggagca cctggcactt ctcatggctg ccatttccca ggcctccagg
     8341 aaacactagg gttataagta gaatcatgtc attttgagtt tttagttttt gaaccatagc
     8401 ctttgtcaat atttgttttg ttttaaccac cagaacactt gaaaactcag taaaaatttc
     8461 attaaagaaa agctttcaaa atgtttaaga ataaaaagta gtgtccagtt atagatggtt
     8521 ctgccctgta cctgagagat ctccgtgctc cctagccctg tttctgctct cccttaatta
     8581 ttcccccagc attcccctga ccattctgac cagaccaatc agtgggtgct caacagtcat
     8641 actaactcat ggctgtttcc cttgctactg ctgtaaaatg tctgtttagg actcaggcca
     8701 accttgacaa tctaacagtg aaatggaata gaggacaggg gagggttggt ggaacccagc
     8761 cgtcctctgg agatgggggg cagctctttc cctgatccta tgtggctcta aaacgagcac
     8821 atatgtgaaa gggccttaga atagcaaaaa tagccaagtt ttggccatgg ggacacacat
     8881 gcctgtatgg ggcgagaggt tcactctcaa ttcaatgact gttgccgggc ggtggtgtct
     8941 tacaccttta atcccagcac ttgggatgca gaggcaggcg gatttctgag tttgaggcca
     9001 gcttggtcta cagagtgagt tccagaaacc ctgtctcgaa aaaacgaaaa aaaaagaaat
     9061 tcaaggactg tgtctgggct gcataacaag accttgtctc aacacacaca cacacataca
     9121 cacacacagg ctaaatatta actacatgtg acgtaggcca aagtctctgt aagttgtaat
     9181 ttcataggtc ttgtcaggtg tatttcagca gtctctggct tcagcaagtg cgccattaat
     9241 tctgacttga tagtgcccag tgagcatggc ctgtgctgcc ttgtggtaga accatgcgta
     9301 tatacatcaa agggatcaga ttccttttgt caattttggc atttcagaat ggatttttaa
     9361 acaccagtca aggataggtc agtgttggct gataccacat gcttagactg tgtgagaacc
     9421 cttgtccagt ctccagcacc ttcactttga gagacagtaa aatcctttcc ttcagaagtg
     9481 gtgatcctgc tgtgttctgc aagtctacca aagattcttg tgaggagttt ctcagcgcct
     9541 gtgtgtgcag acatgtaccc tcctgcttcc ttcacggtgt tagctccagc ccctccgctt
     9601 agtcatctgc agagggctaa gacaggaccg caggcagctg gcacaagcaa ctctgttccc
     9661 tttgccaaaa cctccaaagt tagaaatgaa gttgtttgcg tctgtggtga gggaattgac
     9721 cagccattgc ttcagcctcc ttcccgagta agcaccttct tggtgcctgg gtagccctgg
     9781 gaggacagtg gtgacctgca ccacaaaacc tgcaagacat tattttgaat ggaacatttg
     9841 atgtcatttt tagggaacag aaaccaaata acagaggcca aggaccatgc gtatttcaag
     9901 gagggaagat gagcgccctc tgctggcagt atttgtagtt gattgagtga ggagctctaa
     9961 gcagcctgga aggagtgctg aggtcagcag gcctcaggaa tccttaacat gcaaacaatc
    10021 ttgaggggaa accagaaggt cattcctagc agttactgta atacctctca ttgcaggctg
    10081 ttgggtttct gtcgttcact cggtgtgatt tatcttatgt atctgatagt ttttatggat
    10141 aattgtttgg gttgtaaact cctatactct ttattaaaat gaacctaatt aagaga
//
