LOCUS       NM_030210               3175 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus acetoacetyl-CoA synthetase (Aacs), mRNA.
ACCESSION   NM_030210
VERSION     NM_030210.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Hasegawa,S., Imai,M., Yamasaki,M., Takahashi,N. and Fukui,T.
  TITLE     Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in
            neuroblastoma cells
  JOURNAL   Biochem Biophys Res Commun 495 (1), 652-658 (2018)
   PUBMED   29137983
  REMARK    GeneRIF: These results suggest that Sp1 regulates gene expression
            of AACS in Neuro-2a cells and ketone body utilization affects the
            balance of histone acetylation.
REFERENCE   3  (bases 1 to 3175)
  AUTHORS   Hasegawa,S., Inoue,D., Yamasaki,M., Li,C., Imai,M., Takahashi,N.
            and Fukui,T.
  TITLE     Site-specific cleavage of acetoacetyl-CoA synthetase by legumain
  JOURNAL   FEBS Lett 590 (11), 1592-1601 (2016)
   PUBMED   27129883
  REMARK    GeneRIF: Cleavage of AACS by legumain is critical for the
            regulation of enzymatic activity and results in gain-of-function
            changes.
REFERENCE   4  (bases 1 to 3175)
  AUTHORS   Hasegawa,S., Noda,K., Maeda,A., Matsuoka,M., Yamasaki,M. and
            Fukui,T.
  TITLE     Acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, is
            controlled by SREBP-2 and affects serum cholesterol levels
  JOURNAL   Mol Genet Metab 107 (3), 553-560 (2012)
   PUBMED   22985732
  REMARK    GeneRIF: Acetoacetyl-CoA synthetase, a ketone body-utilizing
            enzyme, is controlled by SREBP-2 and affects serum cholesterol
            levels.
REFERENCE   5  (bases 1 to 3175)
  AUTHORS   Hasegawa,S., Kume,H., Iinuma,S., Yamasaki,M., Takahashi,N. and
            Fukui,T.
  TITLE     Acetoacetyl-CoA synthetase is essential for normal neuronal
            development
  JOURNAL   Biochem Biophys Res Commun 427 (2), 398-403 (2012)
   PUBMED   23000407
  REMARK    GeneRIF: These results suggest that AACS is regulated by SREBP-2
            and involves in the normal development of neurons.
REFERENCE   6  (bases 1 to 3175)
  AUTHORS   Hasegawa,S., Ikeda,Y., Yamasaki,M. and Fukui,T.
  TITLE     The role of acetoacetyl-CoA synthetase, a ketone body-utilizing
            enzyme, in 3T3-L1 adipocyte differentiation
  JOURNAL   Biol Pharm Bull 35 (11), 1980-1985 (2012)
   PUBMED   23123469
  REMARK    GeneRIF: Knockdown of AACS inhibits differentiation of 3T3-L1
            adipocytes and suppresses expression of the adipocyte markers,
            peroxisome proliferator-activated receptor gama and CCAAT/enhancer
            binding protein alpha.
REFERENCE   7  (bases 1 to 3175)
  AUTHORS   Hasegawa,S., Yamasaki,M., Inage,T., Takahashi,N. and Fukui,T.
  TITLE     Transcriptional regulation of ketone body-utilizing enzyme,
            acetoacetyl-CoA synthetase, by C/EBPalpha during adipocyte
            differentiation
  JOURNAL   Biochim Biophys Acta 1779 (6-7), 414-419 (2008)
   PUBMED   18514076
  REMARK    GeneRIF: AACS promoter activity was controlled mainly by C/EBPalpha
            during adipogenesis.
REFERENCE   8  (bases 1 to 3175)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   9  (bases 1 to 3175)
  AUTHORS   Ohgami,M., Takahashi,N., Yamasaki,M. and Fukui,T.
  TITLE     Expression of acetoacetyl-CoA synthetase, a novel cytosolic ketone
            body-utilizing enzyme, in human brain
  JOURNAL   Biochem Pharmacol 65 (6), 989-994 (2003)
   PUBMED   12623130
REFERENCE   10 (bases 1 to 3175)
  AUTHORS   Gu,T., Orita,S. and Han,M.
  TITLE     Caenorhabditis elegans SUR-5, a novel but conserved protein,
            negatively regulates LET-60 Ras activity during vulval induction
  JOURNAL   Mol Cell Biol 18 (8), 4556-4564 (1998)
   PUBMED   9671465
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC119268.12.
            
            On Aug 16, 2018 this sequence version replaced NM_030210.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK044017.1, AK165418.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-237               AC119268.12        10489-10725
            238-341             AC119268.12        17446-17549
            342-462             AC119268.12        19153-19273
            463-576             AC119268.12        24399-24512
            577-674             AC119268.12        29523-29620
            675-789             AC119268.12        37799-37913
            790-871             AC119268.12        38165-38246
            872-1019            AC119268.12        40765-40912
            1020-1100           AC119268.12        43293-43373
            1101-1225           AC119268.12        44395-44519
            1226-1290           AC119268.12        46256-46320
            1291-1413           AC119268.12        46462-46584
            1414-1527           AC119268.12        47241-47354
            1528-1653           AC119268.12        47718-47843
            1654-1723           AC119268.12        48535-48604
            1724-1782           AC119268.12        48785-48843
            1783-1985           AC119268.12        49544-49746
            1986-3175           AC119268.12        50832-52021
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 64.24 cM"
     gene            1..3175
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /note="acetoacetyl-CoA synthetase"
                     /db_xref="GeneID:78894"
                     /db_xref="MGI:MGI:1926144"
     exon            1..237
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..2123
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /EC_number="6.2.1.16"
                     /codon_start=1
                     /product="acetoacetyl-CoA synthetase"
                     /protein_id="NP_084486.1"
                     /db_xref="CCDS:CCDS19687.1"
                     /db_xref="GeneID:78894"
                     /db_xref="MGI:MGI:1926144"
                     /translation="MSKLARLEREEIMECQVMWEPDSKKDTQMDRFRAAVGTACGLAL
                     GNYNDLYHWSVRSYMDFWAEFWKFSGIVYSRMYDEVVDTSKGIADVPEWFRGSRLNYA
                     ENLLRHKENDRVALYVAREGREEIVKVTFEELRQQVALFAAAMRKMGVKKGDRVVGYL
                     PNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHG
                     HLEKLQRVVKGLPDLQRVVLIPYVLPREKIDISKIPNSVFLDDFLASGTGAQAPQLEF
                     EQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHMLHGNMTSSDILLYYTTVG
                     WMMWNWMVSALATGASLVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKD
                     MKPVETHNLHTLHTILSTGSPLKAQSYEYVYRCIKSSVLLGSISGGTDIISCFMGQNS
                     SIPVYKGEIQARNLGMAVEAWDEEGKAVWGASGELVCTKPIPCQPTHFWNDENGSKYR
                     KAYFSKFPGVWAHGDYCRINPKTGGIIMLGRSDGTLNPNGVRFGSSEIYNIVEAFDEV
                     EDSLCVPQYNRDGEERVVLFLKMASGHTFQPDLVKRIRDAIRLGLSARHVPSLILETR
                     GIPYTLNGKKVEVAVKQVMAGRTVEHRGAFSNPETLDLYRDIPELQDF"
     exon            238..341
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            342..462
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            463..576
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            577..674
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            675..789
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            790..871
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1019
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1100
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1225
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1290
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1413
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1527
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1653
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1723
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1782
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1985
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..3175
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3149..3154
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /note="hexamer: AATAAA"
     polyA_site      3175
                     /gene="Aacs"
                     /gene_synonym="2210408B16Rik; SUR5"
                     /note="major polyA site"
ORIGIN      
        1 agtctcgcgc tgtggttcgt cggcgcaccg ctgatccgct ccacgccttg cgctctccgc
       61 tctcagccaa agcccggcag ccccggccac gcagctccgc aaccatgtcc aagctggcgc
      121 ggctcgagcg cgaggagatc atggagtgcc aggtgatgtg ggagcctgac agcaagaagg
      181 acacgcagat ggaccgcttc cgggcggccg tgggtaccgc ctgcggcctg gcgcttggga
      241 attacaatga cttataccac tggtctgtcc ggtcgtatat ggacttttgg gctgagttct
      301 ggaagttcag tggaatcgtc tactcacgca tgtatgatga ggttgtggac acatccaaag
      361 ggattgcaga tgtccccgag tggttcagag gcagccgcct caactacgca gagaacctcc
      421 tgcggcacaa ggagaacgac agagtcgccc tttacgtggc ccgggaaggc agagaggaga
      481 tcgtgaaggt gacttttgaa gagctgcggc agcaggtggc tctgttcgca gctgccatga
      541 ggaagatggg cgtgaagaaa ggggaccgtg tggtcggcta tctacccaac agtgcgcatg
      601 cggtggaggc catgctggct gctgccagta ttggggccat ttggagttct acctcgccgg
      661 actttggtgt aaatggtgtc ctggaccgct tttctcaaat tcagccgaaa cttatcttct
      721 cggtggaggc tgttgtctac aatggcaagg agcatggtca cctggagaag ctgcagcgag
      781 ttgtgaaagg actgcctgac ctgcagcgag tggtgctgat cccctatgtc ctcccaaggg
      841 agaagattga catttccaag atccccaaca gtgtgttcct ggatgatttc ctggcaagcg
      901 ggaccggcgc gcaggcgccg cagctcgagt ttgagcagct gcccttcagc caccctctgt
      961 tcatcatgtt ctcctcaggc acgacgggag cgcccaagtg catggtgcac tcagccgggg
     1021 gcaccctcat ccagcacctg aaggagcaca tgctacacgg caacatgaca agcagtgaca
     1081 tcctgctcta ctacaccacg gtcggctgga tgatgtggaa ctggatggtg tcagccctgg
     1141 ccacaggagc atccttggtc ctgtatgatg gctccccgct ggttccgacc cccaacgtgt
     1201 tgtgggacct tgtggacagg atagggatca ccatcctggg aacgggagcc aagtggctgt
     1261 cagtgctgga ggagaaggac atgaagccag tggaaactca caacctccac acgctgcaca
     1321 cgatcctgtc caccggctcg ccgctgaaag cccagagtta cgagtatgtg tacagatgca
     1381 tcaagagctc cgtgctcctg ggctccatct caggaggcac tgacatcatc tcctgtttca
     1441 tgggccagaa ctcctctatt cctgtgtaca agggtgagat ccaagcccgg aaccttggca
     1501 tggctgtgga agcctgggac gaggaaggga aggccgtctg gggagcgagt ggcgagctgg
     1561 tgtgcactaa gcccattccc tgccagccca cgcacttctg gaacgacgag aacggcagca
     1621 agtaccggaa ggcttacttc tccaaattcc caggtgtctg ggcacacggt gactactgca
     1681 ggatcaaccc caaaacagga ggcattatca tgctgggccg tagtgatggc accctcaacc
     1741 ccaatggcgt ccgctttggc agctcggaga tctacaacat cgtggaagcc ttcgatgagg
     1801 tggaggacag cctgtgtgta ccccagtaca acagagatgg cgaggagcgg gtggtcctgt
     1861 tcctgaagat ggcgtccggg cacactttcc agcctgacct cgtgaagcgc atccgagacg
     1921 ccatccgact tggcctgtct gcccgccatg tgcccagcct catcctggag acccgaggca
     1981 ttccatacac actcaatggc aagaaagtgg aggtggccgt gaagcaggtg atggctggga
     2041 ggactgtgga gcaccggggg gccttctcca accccgagac cctcgacctg taccgggaca
     2101 tccctgagct gcaggacttc tgagccagca gctcgcactc catccagctg tgcatgtgat
     2161 ggaacttagg gacactttag agacaacagc tgctccaggt ggccctggca ctgcacactc
     2221 cacaggctca ggaacagctt tcctctttcg gagtcattgg tgggggccag gtctcccgag
     2281 ctccaggatg tgcctggcct tcggagaccc taagcataca ctgaggcttg tctcggccct
     2341 gctgctatag gtcagcacac agccttgcag gggcagggct ggtgacattt ggtgacagca
     2401 cactggagga gcgatgtggc cttcagcctc atgagcccgc tgttgtatct cgctctatga
     2461 aggtgaagtc ttacctatac tctctgtcct taactggcct ggcttcctca gcgttactgt
     2521 cttctcaaga gctgactaaa gccagggcac ctggcccagc atgcctgtca cctcacagct
     2581 ggctgttggg acacctgggc tgacggctcc tgtaccgtct accaaggcca gaacaccatt
     2641 ctagggaatg gtgagtgaat tgtccagaca gccaagagct ctctgctatg gcaagtcccc
     2701 tctgctgtcg ggtgtccccg actgtttctg ggtcttacca gcacagtgtt tgaatgggtc
     2761 aaacggttta atgttaattg agggcctggg ggcacttgaa tccctgggtg tctaccccag
     2821 gttaccactt agctgtgtgc tgggcagtgt gatcctggag tccgccaggg atggagctgc
     2881 cccgcctcag agggaaatgg ccccgtgctc cctcctaata gttcctgggc tctttctatt
     2941 gtgactgtcc tctacatttg taaatgaagt ctcaggcttt ggctgccagg acttaatggt
     3001 gcttctcctc tagcacacgg aatatgtcat aaatcctggt gattgctttt gtattttttt
     3061 ttttaaaaac tgagcacaac ataaaacctt ttaaagatat cctggatctt aagtctataa
     3121 aggaaaagtg ctatgaagaa ttttatggaa taaatctgtg ccatgcacac ctgta
//
