LOCUS       NM_001077424            2757 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Mus musculus alpha-1,4-N-acetylglucosaminyltransferase (A4gnt),
            mRNA.
ACCESSION   NM_001077424 XM_286168 XM_907314 XM_992622
VERSION     NM_001077424.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Iida,M., Desamero,M.J., Yasuda,K., Nakashima,A., Suzuki,K.,
            Chambers,J.K., Uchida,K., Ogawa,R., Hachimura,S., Nakayama,J.,
            Kyuwa,S., Miura,K., Kakuta,S. and Hirayama,K.
  TITLE     Effects of orally administered Euglena gracilis and its reserve
            polysaccharide, paramylon, on gastric dysplasia in A4gnt knockout
            mice
  JOURNAL   Sci Rep 11 (1), 13640 (2021)
   PUBMED   34210998
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Tan,S.H., Swathi,Y., Tan,S., Goh,J., Seishima,R., Murakami,K.,
            Oshima,M., Tsuji,T., Phuah,P., Tan,L.T., Wong,E., Fatehullah,A.,
            Sheng,T., Ho,S.W.T., Grabsch,H.I., Srivastava,S., Teh,M.,
            Denil,S.L.I.J., Mustafah,S., Tan,P., Shabbir,A., So,J., Yeoh,K.G.
            and Barker,N.
  TITLE     AQP5 enriches for stem cells and cancer origins in the distal
            stomach
  JOURNAL   Nature 578 (7795), 437-443 (2020)
   PUBMED   32025032
REFERENCE   3  (bases 1 to 2757)
  AUTHORS   Kawakubo,M., Komura,H., Goso,Y., Okumura,M., Sato,Y., Fujii,C.,
            Miyashita,M., Arisaka,N., Harumiya,S., Yamanoi,K., Yamada,S.,
            Kakuta,S., Kawashima,H., Fukuda,M.N., Fukuda,M. and Nakayama,J.
  TITLE     Analysis of A4gnt Knockout Mice Reveals an Essential Role for
            Gastric Sulfomucins in Preventing Gastritis Cystica Profunda
  JOURNAL   J Histochem Cytochem 67 (10), 759-770 (2019)
   PUBMED   31246144
REFERENCE   4  (bases 1 to 2757)
  AUTHORS   Karasawa,F., Shiota,A., Goso,Y., Kobayashi,M., Sato,Y.,
            Masumoto,J., Fujiwara,M., Yokosawa,S., Muraki,T., Miyagawa,S.,
            Ueda,M., Fukuda,M.N., Fukuda,M., Ishihara,K. and Nakayama,J.
  TITLE     Essential role of gastric gland mucin in preventing gastric cancer
            in mice
  JOURNAL   J Clin Invest 122 (3), 923-934 (2012)
   PUBMED   22307328
  REMARK    GeneRIF: A4gnt(-/-) mice show complete lack of alphaGlcNAc
            expression in gastric gland mucin
REFERENCE   5  (bases 1 to 2757)
  AUTHORS   Zheng,Z., Jia,Y., Hou,L., Persson,C., Yeager,M., Lissowska,J.,
            Chanock,S.J., Blaser,M., Chow,W.H. and Ye,W.
  TITLE     Genetic variation in a4GnT in relation to Helicobacter pylori
            serology and gastric cancer risk
  JOURNAL   Helicobacter 14 (5), 120-125 (2009)
   PUBMED   19751437
  REMARK    GeneRIF: This publication contains functional information for the
            human homolog of mouse A4gnt.
REFERENCE   6  (bases 1 to 2757)
  AUTHORS   Nakayama,J., Yeh,J.C., Misra,A.K., Ito,S., Katsuyama,T. and
            Fukuda,M.
  TITLE     Expression cloning of a human alpha1,
            4-N-acetylglucosaminyltransferase that forms
            GlcNAcalpha1-->4Galbeta-->R, a glycan specifically expressed in the
            gastric gland mucous cell-type mucin
  JOURNAL   Proc Natl Acad Sci U S A 96 (16), 8991-8996 (1999)
   PUBMED   10430883
  REMARK    GeneRIF: This publication contains functional information for the
            human homolog of mouse A4gnt.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK136425.1 and AC157949.1.
            
            On Jan 7, 2010 this sequence version replaced NM_001077424.1.
            
            Summary: This gene encodes a protein from the glycosyltransferase
            32 family. The encoded enzyme catalyzes the transfer of
            N-acetylglucosamine to alpha-1,4-linked beta-galactose residues.
            This enzyme is required for type III mucin synthesis and it is
            largely associated with the Golgi apparatus membrane. The encoded
            protein appears to be expressed in adenocarcinoma cells of
            pancreatic, biliary tract and gastric cancers.[provided by RefSeq,
            Jan 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK136425.1, BY708771.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-233               AK136425.1         2-234
            234-831             AK136425.1         236-833
            832-886             AC157949.1         166387-166441       c
            887-2559            AK136425.1         888-2560
            2560-2757           AC157949.1         164516-164713       c
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 51.91 cM"
     gene            1..2757
                     /gene="A4gnt"
                     /gene_synonym="Alpha4gnt; Gm798"
                     /note="alpha-1,4-N-acetylglucosaminyltransferase"
                     /db_xref="GeneID:333424"
                     /db_xref="MGI:MGI:2143261"
     exon            1..100
                     /gene="A4gnt"
                     /gene_synonym="Alpha4gnt; Gm798"
                     /inference="alignment:Splign:2.1.0"
     exon            101..589
                     /gene="A4gnt"
                     /gene_synonym="Alpha4gnt; Gm798"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..1204
                     /gene="A4gnt"
                     /gene_synonym="Alpha4gnt; Gm798"
                     /codon_start=1
                     /product="alpha-1,4-N-acetylglucosaminyltransferase"
                     /protein_id="NP_001070892.1"
                     /db_xref="CCDS:CCDS40740.1"
                     /db_xref="GeneID:333424"
                     /db_xref="MGI:MGI:2143261"
                     /translation="MLKEIYLSLSLVLVFACGLLYQLTMRSQCFFACLPPFSFPQGLD
                     GLLRSGRSIMFIETSERVEPPPMVSCAVESAAKIYPEQPIIFFMKGLRDSVQLTSNTS
                     YPAFSLLSAINNVFFVPLDMERLFKDTPLFSWYTKVNSSTEKHWLHVSSDAARLAIIW
                     KYGGIYMDTDVISLQPIPEENFLAAQGSRHSSNGVFGFLPHHPFLWACMENFVEHYDS
                     TIWGNQGPQLMTRMLRVWCRLKDFHGLGDLKCLNISFLHPQRFYPIPYPQWKRYYQVW
                     DKEPSFNESYALHLWNYMNKEGKTVVRGSKTLVENLYQKHCPKTYRVLIQGAEGTVSK
                     KPGTGSR"
     exon            590..2757
                     /gene="A4gnt"
                     /gene_synonym="Alpha4gnt; Gm798"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaagattag gcagtgagtt accagactgc atacaggaaa aaaagtgtcc tcacttctaa
       61 gacttcaaat actggtgaac tgcaagtctc tgactgaaaa gttgtctacg tcaggatcac
      121 cagggcgact tctaatgaac tctgttgctt gaaggagagg ctggcagggc tggaggacat
      181 gctgaaggaa atttatctct ccctgtccct ggtcctggtg tttgcctgtg gcctgctgta
      241 ccagctcacc atgaggtctc agtgcttctt tgcctgcctg cctcctttct catttccaca
      301 gggactggac ggcctcctga ggagtggacg aagcatcatg tttatagaga cctctgaaag
      361 ggtggagcca cctccgatgg tctcctgtgc tgtggagtct gctgctaaaa tctatcctga
      421 gcagcccatc atcttcttca tgaaaggact cagggactcc gtacagctga cttcaaatac
      481 cagctaccca gccttctccc tcctctcggc cattaataat gttttctttg tgcctttgga
      541 catggaaaga ctgttcaaag acacaccctt gttttcgtgg tacacaaaag tcaacagcag
      601 tacagagaaa cactggctcc acgtcagctc cgacgcagcc cgcctggcca tcatctggaa
      661 gtacggaggc atctacatgg acactgacgt catctccctt cagcccatcc ctgaggagaa
      721 cttcctggca gcccagggct cccgacactc cagtaacggg gtatttgggt tcctccccca
      781 ccaccccttc ctgtgggcct gcatggagaa ctttgtagag cactatgact caaccatttg
      841 gggcaaccaa ggtccccagt tgatgacaag gatgttacgg gtgtggtgtc gtcttaaaga
      901 cttccacggg ttgggtgacc tgaagtgttt gaatatttct ttccttcatc cccagagatt
      961 ttaccccatc ccttatccac agtggaagcg ctactaccaa gtgtgggaca aagagcccag
     1021 cttcaatgag tcctacgcac tgcatttgtg gaactatatg aataaagagg gcaagactgt
     1081 ggttagagga agcaaaactc tggtggaaaa tctctatcaa aagcactgtc ctaagactta
     1141 tagggttctg attcaaggtg cagaagggac agtgtccaag aagccaggca caggttccag
     1201 gtagagagat gttgctactg tggaagagta gatgcttcct gggctacccc attctcgctt
     1261 ggtctacatt gtctctaggg gataggtgct aatccacacc cccaggatta actttcctgg
     1321 ctataactgt atgcttccaa aggaaagcgg ctgcaaacac agaagttgat ttatccttca
     1381 tggaacagca agtctggggg cagataattt agggcagggt atatgggttc ctcccccact
     1441 gtaaattaac actggccgtt ggaagcccat gctagaatct ttctgtttgt tttcctgctg
     1501 tctatagtcc atgctcatgt ggctgctgga cctacagctc atcatttggg ctattccaga
     1561 cagtaggatg acagaagaag gaaagaaaat gcatctcata cgactttatg catgtacggt
     1621 ataagtgcaa aaaccatgta tccttgtatc tcccttggct agattgtagc tgcaagaaag
     1681 actgggaggt agctaagagc tctgtttact gttctattgt cattaaatat taagattaat
     1741 tttaacacct gtcagaatga agggacctgg ctgcttgttg ttttgttttg atttttgaga
     1801 tagggtctca ctatgaagat caggatagta tccaactcac cgagatcctc ctgcctctac
     1861 ctcccttatg ctgggattaa aggcatgtga catccatcac acctggccct tgatttagac
     1921 attaatgtat taatccattt tctgtcactg taacaaaata tccaaactgg gaaatttatt
     1981 aagaaaagat atttatagaa ctggagactg agctcttgac ccagatgact ctgctctagt
     2041 gaagccatcg tggagagtgg catcacagtg acagggaaca tgtcacatgg cgagtcagaa
     2101 agccagagga gctcaggaac aagtcgtgcc ccctccccgc ctcctcatcc ctagagagct
     2161 tggtgtcaga gggaaggtct tgctgtgtcc caggcaggcc tcaaactctc aatccttttt
     2221 cctaaggaaa aaggggctgg gattacaggt atgtgccacc atagcccact caggcttcct
     2281 ctttttatag gaactaactg gaatcccatc aaaagtacat taactccttg tgatggaatc
     2341 ctcccatgac atggagactg ggctagaaat acacaaaacc aggtataaac tacagcaatg
     2401 ttcattaact cattaactca gtacatacta attgatcttc ctgggtacca agcactaatt
     2461 tagggaacaa agatgcaggt ggaccaagaa gccttggtcc cagcacatgt atcctaattc
     2521 ctggtcactt gtcttcagga tactgtctcc cttcacaggc tgccttcccc acacttcccc
     2581 tttccaacac acacctttat cttactaccc cagctgttga ttaagagcaa attctaagct
     2641 caaactattt taatctgctt tgtccataga tgcaaatgaa attgaatttc tccagcatca
     2701 tgttgttcat atgtgatttt tattgttcaa ttataaataa actcatttct gattttc
//
