LOCUS       NM_001004150            2047 bp    mRNA    linear   ROD 04-OCT-2024
DEFINITION  Mus musculus alpha 1,4-galactosyltransferase (A4galt), transcript
            variant 2, mRNA.
ACCESSION   NM_001004150 XM_139501 XM_488751
VERSION     NM_001004150.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Sharma,P., Zhang,X., Ly,K., Zhang,Y., Hu,Y., Ye,A.Y., Hu,J.,
            Kim,J.H., Lou,M., Wang,C., Celuzza,Q., Kondo,Y., Furukawa,K.,
            Bundle,D.R., Furukawa,K., Alt,F.W. and Winau,F.
  TITLE     The lipid globotriaosylceramide promotes germinal center B cell
            responses and antiviral immunity
  JOURNAL   Science 383 (6684), eadg0564 (2024)
   PUBMED   38359115
REFERENCE   3  (bases 1 to 2047)
  AUTHORS   Bowen,E.E., Hurcombe,J.A., Barrington,F., Keir,L.S., Farmer,L.K.,
            Wherlock,M.D., Ortiz-Sandoval,C.G., Bruno,V.,
            Bohorquez-Hernandez,A., Diatlov,D., Rostam-Shirazi,N., Wells,S.,
            Stewart,M., Teboul,L., Lay,A.C., Butler,M.J., Pope,R.J.P.,
            Larkai,E.M.S., Morgan,B.P., Moppett,J., Satchell,S.C., Welsh,G.I.,
            Walker,P.D., Licht,C., Saleem,M.A. and Coward,R.J.M.
  TITLE     Shiga toxin targets the podocyte causing hemolytic uremic syndrome
            through endothelial complement activation
  JOURNAL   Med 4 (11), 761-777 (2023)
   PUBMED   37863058
REFERENCE   4  (bases 1 to 2047)
  AUTHORS   Gao,S., Ni,C., Huang,W., Hao,H., Jiang,H., Lv,Q., Zheng,Y., Liu,P.,
            Kong,D. and Jiang,Y.
  TITLE     The interaction between flagellin and the glycosphingolipid Gb3 on
            host cells contributes to Bacillus cereus acute infection
  JOURNAL   Virulence 11 (1), 769-780 (2020)
   PUBMED   32507026
  REMARK    GeneRIF: The interaction between flagellin and the
            glycosphingolipid Gb3 on host cells contributes to Bacillus cereus
            acute infection.
REFERENCE   5  (bases 1 to 2047)
  AUTHORS   Hamamura,K., Hamajima,K., Yo,S., Mishima,Y., Furukawa,K.,
            Uchikawa,M., Kondo,Y., Mori,H., Kondo,H., Tanaka,K., Miyazawa,K.,
            Goto,S. and Togari,A.
  TITLE     Deletion of Gb3 Synthase in Mice Resulted in the Attenuation of
            Bone Formation via Decrease in Osteoblasts
  JOURNAL   Int J Mol Sci 20 (18), 4619 (2019)
   PUBMED   31540393
  REMARK    GeneRIF: The deficiency of Gb3 synthase in mice decreased the
            number of osteoblasts on the bone surface, and suppressed mRNA
            levels of osteogenic differentiation markers.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2047)
  AUTHORS   Kondo,Y., Tokuda,N., Fan,X., Yamashita,T., Honke,K., Takematsu,H.,
            Togayachi,A., Ohta,M., Kotzusumi,Y., Narimatsu,H., Tajima,O.,
            Furukawa,K. and Furukawa,K.
  TITLE     Glycosphingolipids are not pivotal receptors for Subtilase
            cytotoxin in vivo: sensitivity analysis with
            glycosylation-defective mutant mice
  JOURNAL   Biochem Biophys Res Commun 378 (2), 179-181 (2009)
   PUBMED   18996356
  REMARK    Erratum:[Biochem Biophys Res Commun. 2009 Jun 26;384(2):275.
            Takematsu, Hiroshi [corrected to Takematsu, Hiromu]; Furukaw, Keiko
            [corrected to Furukawa, Keiko]]
REFERENCE   7  (bases 1 to 2047)
  AUTHORS   Porubsky,S., Luckow,B., Bonrouhi,M., Speak,A., Cerundolo,V.,
            Platt,F. and Grone,H.J.
  TITLE     [Glycosphingolipids Gb3 and iGb3. In vivo roles in hemolytic-uremic
            syndrome and iNKT cell function]
  JOURNAL   Pathologe 29 Suppl 2, 297-302 (2008)
   PUBMED   18810447
  REMARK    GeneRIF: Verification of the function of Gb3 in the pathogenesis of
            hemolytic-uremic syndrome as the cellular receptor responsible for
            cytotoxicity caused by verotoxin (VT) elaborated by Shigella and
            certain strains of E.coli.
REFERENCE   8  (bases 1 to 2047)
  AUTHORS   Okuda,T., Tokuda,N., Numata,S., Ito,M., Ohta,M., Kawamura,K.,
            Wiels,J., Urano,T., Tajima,O., Furukawa,K. and Furukawa,K.
  TITLE     Targeted disruption of Gb3/CD77 synthase gene resulted in the
            complete deletion of globo-series glycosphingolipids and loss of
            sensitivity to verotoxins
  JOURNAL   J Biol Chem 281 (15), 10230-10235 (2006)
   PUBMED   16476743
  REMARK    GeneRIF: Gb3/CD77 synthase has a role in synthesis of globo-series
            glycosphingolipids and sensitivity to verotoxins
REFERENCE   9  (bases 1 to 2047)
  AUTHORS   Fujii,Y., Numata,S., Nakamura,Y., Honda,T., Furukawa,K., Urano,T.,
            Wiels,J., Uchikawa,M., Ozaki,N., Matsuo,S., Sugiura,Y. and
            Furukawa,K.
  TITLE     Murine glycosyltransferases responsible for the expression of
            globo-series glycolipids: cDNA structures, mRNA expression, and
            distribution of their products
  JOURNAL   Glycobiology 15 (12), 1257-1267 (2005)
   PUBMED   16079416
  REMARK    GeneRIF: Gb3 and Gb4 synthase are responsible for the expression of
            globo-series glycolipids
REFERENCE   10 (bases 1 to 2047)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL591952.9.
            
            On May 17, 2019 this sequence version replaced NM_001004150.3.
            
            Summary: The protein encoded by this gene catalyzes the transfer of
            galactose to lactosylceramide to form globotriaosylceramide, which
            has been identified as the P(k) antigen of the P blood group
            system. The encoded protein, which is a type II membrane protein
            found in the Golgi, is also required for the synthesis of the
            bacterial verotoxins receptor. Alternative splicing results in
            multiple transcript variants.[provided by RefSeq, Jan 2010].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. All three variants encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY371179.1, AK149186.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AL591952.9         29676-29705
            31-174              AL591952.9         50748-50891
            175-2047            AL591952.9         52811-54683
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 39.4 cM"
     gene            1..2047
                     /gene="A4galt"
                     /note="alpha 1,4-galactosyltransferase"
                     /db_xref="GeneID:239559"
                     /db_xref="MGI:MGI:3512453"
     exon            1..30
                     /gene="A4galt"
                     /inference="alignment:Splign:2.1.0"
     exon            31..174
                     /gene="A4galt"
                     /inference="alignment:Splign:2.1.0"
     exon            175..2047
                     /gene="A4galt"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..1268
                     /gene="A4galt"
                     /EC_number="2.4.1.228"
                     /note="Gb3 synthase; Gb3/CD77 synthase;
                     UDP-galactose:beta-D-galactosyl-beta1-R
                     4-alpha-D-galactosyltransferase;
                     alpha-1,4-N-acetylglucosaminyltransferase; alpha4Gal-T1;
                     globotriaosylceramide synthase"
                     /codon_start=1
                     /product="lactosylceramide 4-alpha-galactosyltransferase"
                     /protein_id="NP_001004150.2"
                     /db_xref="GeneID:239559"
                     /db_xref="MGI:MGI:3512453"
                     /translation="MSKPPDCLLRMLRGTPRQRVFTFFIISFKFMFLISILIYWHTVG
                     APKDQREYSLPVDFSCPQLAFPRVSAPGNIFFLETSDRTSPNFLFMCSVESAARAHPE
                     SQVVVLMKGLPRDTTAQPRNLGISLLSCFPNVWIRPLDLQELFEDTPLAAWYSEARHR
                     WEPYQLPVLSDASRIALLWKFGGIYLDTDFIVLKNLLNLTNTLGIQSRYVLNGAFLAF
                     ERKHEFLALCLHDFVANYNGWIWGHQGPQLLTRVFKKWCSIQSLEKSHACRGVTALPP
                     EAFYPIPWQNWKKYFEDISPEELTQLLNATYAVHVWNKKSQGTHLEATSKALLAQLHA
                     RYCPTTHRAMKMYL"
     regulatory      2027..2032
                     /regulatory_class="polyA_signal_sequence"
                     /gene="A4galt"
                     /note="hexamer: AATAAA"
     polyA_site      2047
                     /gene="A4galt"
                     /note="major polyA site"
ORIGIN      
        1 agtagccgcc cgccgacgcg ggagccggag gtgggtgtga caggcctgag catgagagga
       61 caagctatat actccggttg gcacagaaga ctagttccta acccagtgtg catgtccagg
      121 atgcctgcag gctgctagga cccctcacct ccttgttatg gactgcccag gctgacaagt
      181 caatctttat gggcatctcc tgttcccatc tggaggagac catgtccaag ccccccgact
      241 gcctgctgcg gatgctcagg ggcaccccca gacagcgggt cttcaccttc ttcatcatca
      301 gcttcaagtt catgttcttg atctccatcc tgatctactg gcacactgtg ggtgcaccca
      361 aggaccaaag agagtatagc ctgccagtag acttctcctg cccccagctg gcctttccta
      421 gagtctctgc cccaggcaac atcttcttcc tagagacatc ggacaggacc agtcccaact
      481 ttctatttat gtgttctgtg gaatcagctg ccagggcaca ccctgaatcc caggtggttg
      541 tgctgatgaa agggctgcct agggacacca cagctcagcc ccggaacctc ggcatctctc
      601 ttctgagctg ttttcctaat gtctggatac gacctctgga cttgcaagaa ctgtttgagg
      661 acacaccact ggcagcctgg tactcggagg cacggcacag atgggagccc taccagttgc
      721 ccgtactgtc tgatgcttcc aggatcgcac tcctctggaa gtttggtggg atctacctgg
      781 acacagactt catcgtcctc aagaacctgc tcaacctgac caacacgctg ggcatccaat
      841 cccgctatgt cctcaacggt gccttcctag ccttcgagcg caagcatgag ttcttggcac
      901 tgtgtctaca tgacttcgtg gccaactaca atggttggat ttggggtcac cagggccccc
      961 aactgcttac ccgagtcttc aagaagtggt gttccatcca aagcctggaa aagagccatg
     1021 cttgccgtgg ggtcactgcc ctgccccccg aggccttcta ccccatcccc tggcagaact
     1081 ggaagaaata ctttgaagac atcagccccg aggagctgac ccagctgctc aatgccacct
     1141 atgctgttca tgtgtggaac aagaaaagtc agggtacaca cctagaggcc acatccaagg
     1201 ctctgctggc ccagctacat gcccgctatt gccctacgac acatagagcc atgaagatgt
     1261 acttgtgaca gcccttcagg ctgcagtcat atcatatcaa cattgttacc gcctttccag
     1321 ggagacaaga tgagcagaca gggaaaaagg ggcctttgag atgctgctgt tgactttaga
     1381 ggagaccaag ctggcctggg gaacacttgc cagttctaca cctgcccctc taagccaaag
     1441 ggagaccgat ggagaacact ttgtccggtg ttctaggctg accagtgatt acagaattac
     1501 taacatctgg ggccttaatg ggctagatca caagaacact aaagagcatt ctaaggtagc
     1561 tggaagacag ctggttagag gtggggtcag gagcggagct ctgaaaacct gtgtagaaat
     1621 gctgagggag aaggacctgg ctctctcagc ctcatgagcc caaggggagg ggcagggctc
     1681 cctgggaaca ccatctttcc tcccctggga cagtgctctg aagatctatc acagcctgtt
     1741 ccagtgttcg gggatgtgga cttttttttt ttttttccga gacagggttt ctctgtgtag
     1801 ccctggctgt cctggaactc actctgtaga ccaggctggc ctcgaactca gaagccctcc
     1861 cagtgctggg attaaaggcg tatgccacca ctgcctggct tttttttttt ttttaatttt
     1921 ttaatttttt atttttattt ttattttggt ttgtggaaat tctttaggtt ttaaaaaagg
     1981 aaaaagagtt ttaaactccc tccctgttgt agacactggg ggctctaata aaagtgactc
     2041 accaaca
//
