LOCUS       NM_001376944            3147 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus RIKEN cDNA 4930550C14 gene (4930550C14Rik), transcript
            variant 3, mRNA.
ACCESSION   NM_001376944 XM_006510652
VERSION     NM_001376944.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Lee,J., Pappalardo,Z., Chopra,D.G., Hennings,T.G., Vaughn,I.,
            Lan,C., Choe,J.J., Ang,K., Chen,S., Arkin,M., McManus,M.T.,
            German,M.S. and Ku,G.M.
  TITLE     A Genetic Interaction Map of Insulin Production Identifies Mfi as
            an Inhibitor of Mitochondrial Fission
  JOURNAL   Endocrinology 159 (9), 3321-3330 (2018)
   PUBMED   30059978
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC079869.22.
            
            On Dec 5, 2019 this sequence version replaced XM_006510652.4.
            
            Transcript Variant: Variants 1, 2 and 3 encode the same isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.192766.1,
                                           SRR5189679.148555.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-327               AC079869.22        129423-129749       c
            328-417             AC079869.22        126893-126982       c
            418-510             AC079869.22        123196-123288       c
            511-564             AC079869.22        120643-120696       c
            565-765             AC079869.22        113266-113466       c
            766-896             AC079869.22        112017-112147       c
            897-1067            AC079869.22        109342-109512       c
            1068-1123           AC079869.22        106845-106900       c
            1124-3147           AC079869.22        100609-102632       c
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 29.12 cM"
     gene            1..3147
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /note="RIKEN cDNA 4930550C14 gene"
                     /db_xref="GeneID:75311"
                     /db_xref="MGI:MGI:1922561"
     exon            1..327
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /inference="alignment:Splign:2.1.0"
     exon            328..417
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /inference="alignment:Splign:2.1.0"
     CDS             337..1284
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /note="isoform 1 is encoded by transcript variant 3;
                     uncharacterized protein C11orf65 homolog; mitochondrial
                     fission factor interactor; protein MFI"
                     /codon_start=1
                     /product="protein MFI isoform 1"
                     /protein_id="NP_001363873.1"
                     /db_xref="GeneID:75311"
                     /db_xref="MGI:MGI:1922561"
                     /translation="MSVKHIQDNSNQEIAARIIQRAWKTFLNVSVYQHFKSLIDLRRQ
                     GEPRQIVRYINPKEAQLLDAAAGVQVRFRLGGVRFPPEIYYKIFTHRHIEDLCANSPR
                     DYTKLPARYTSHNKDDPPQVEDNSGWYRRVENNGWRPVSYRFWMPLESGVVDSTKESE
                     FHFSKLKRKQDLEKKRKIKKIDWMRQMYYMGSLEAKATDNETLGLIHKATKGLIKSIE
                     DGGVDSVMEWEVDEVLNWTNTLNFDEYIASWRETATSNSSANLKDVKLQRIQKSLQSN
                     IYGDEAKQAEESLYDDSTYYENAYTKQFTRLTPDSMFGM"
     exon            418..510
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /inference="alignment:Splign:2.1.0"
     exon            511..564
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /inference="alignment:Splign:2.1.0"
     exon            565..765
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /inference="alignment:Splign:2.1.0"
     exon            766..896
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1067
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1123
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..3147
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1501..1506
                     /regulatory_class="polyA_signal_sequence"
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /note="hexamer: AATAAA"
     polyA_site      1527
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /note="major polyA site"
     regulatory      3065..3070
                     /regulatory_class="polyA_signal_sequence"
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /note="hexamer: AATAAA"
     polyA_site      3081
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
     regulatory      3126..3131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
                     /note="hexamer: AATAAA"
     polyA_site      3147
                     /gene="4930550C14Rik"
                     /gene_synonym="Mfi"
ORIGIN      
        1 aagtcgttac caaggaaaca gcggagagcg acagagattg gcaagtcaag ggacatgttc
       61 cctccccagc tcctttctgc tctggtgtct ggtttgatct tggcctgctg aaagacgcta
      121 aatactcacg atcttagtat gctaccatcc ttctccatgc tctgttcccc aaacccctct
      181 cctggcacag ttgctctgct gtgatggaaa agctttcctc tacctcacca acccccactc
      241 cagcctcgcc ctaactcagg agctgcagct tcggggatat tgctaaccca cctctcgcat
      301 tgggtttcct ccaccctgtt aaatgcaaac ttccaaatgt ctgtgaaaca tatccaggac
      361 aattcaaacc aggaaatagc tgcaagaatc attcagaggg cctggaaaac tttccttaat
      421 gtttccgtat accagcattt taaaagtctg attgacctaa gaagacaagg ggagccacgt
      481 cagatagtga ggtacattaa ccctaaagag gcacagctcc tagatgctgc tgctggcgtc
      541 caagtccgat tcaggttggg tggggttaga tttccacctg agatatacta taaaattttt
      601 actcacagac atattgagga tctgtgtgct aacagcccta gagattacac aaagcttcca
      661 gcaagataca catcccacaa caaagatgat ccaccccagg tggaggacaa cagtggttgg
      721 tatcgccgtg tagagaacaa cggctggagg cctgtttcct acagattttg gatgccactt
      781 gaaagcggag tggtggacag cacaaaggaa agcgaatttc atttctctaa actgaagaga
      841 aagcaagatc tggagaagaa aaggaaaatt aaaaaaattg attggatgag acaaatgtac
      901 tacatgggaa gcctggaggc caaggcaaca gataatgaga ctttaggtct aattcacaaa
      961 gcaacaaaag gcctaatcaa gagtattgaa gatggtggag tggattctgt aatggagtgg
     1021 gaagtagatg aagtgctgaa ctggacaaac acactcaact ttgatgagta tattgcaagt
     1081 tggagagaaa ctgctacaag caactcttca gctaacttaa aagatgtcaa gcttcagcga
     1141 atacagaaaa gcctacagag caacatctat ggcgatgagg caaagcaagc agaggagtca
     1201 ctgtacgatg attctactta ctatgaaaat gcttatacaa aacagttcac tagattaaca
     1261 cccgattcca tgttcggcat gtaaccagaa aggattccct tttctgagcg atggacctac
     1321 cttcaagccc actttccagc gaaagccaag aaactactta ttctgactac aatgacatgt
     1381 gtacgtctta catgtcttac ttactcttag ttaagggata ctgaagaaat actgccctgg
     1441 acagtgtaca gttcctcctg gtattctgat aaactatagt tgtttgacag ttatacttaa
     1501 aataaagata cttttttttg acattaagaa ccttcaaata cagttttgct tgtggggcag
     1561 ttgctcagtg cagggctcac tcactggaca ctccatctcc aaacggctct cacttacttg
     1621 tcttactgaa gtgtccactg attttttttt tcctttttaa ttctgcacag gtaacaattt
     1681 acacatttat ggagaatggt gtatttcaca gtatttatac aacatgtacc gacggagtca
     1741 gggtggccga tgtctgtcca cttggtttag tttggcacct ttgggtgtgt agtgccacag
     1801 tgcatgtaga gtgcacggac gtctttggag atacactaag aagtgacact gctgagccac
     1861 acagcagatt cactttcccc tcatatcaat caacacacac attttgtttg gtttttgata
     1921 atgtgcattg tttatagcat gacagcatct tgtggttttg atggcttaac attttttaaa
     1981 gttttttttt aatgtgttct tgatattgac tctgtagatt aatagatggc aagtatcttt
     2041 acctaaaacc tgtaagctct ctcttattct tatagttttg ttgctccgcg tgagcatttc
     2101 agttggtatg attcccaacc tgccactttt gtgttttctg tgcattaagg gtgcgtcaag
     2161 aaagtcttta ctcccactag catcttgcat ttcctgtgtt cctgtaatag ttctgtctta
     2221 agacctctag gtcttgacac atttgagttg attttggtat agggtgtgac acagggaatc
     2281 aacttttaat cttctgcatt aagatatagt ttatcagcac caatggaaat aaggaaacgg
     2341 tgcctaggta aacatttttt ttccgaacat cagtgtagaa ttagcttagt gaggtatata
     2401 gctttatctc tggggccata aaattttcat tattgtaact caagcatgtc tcgcaatcct
     2461 ttcaagtgtt ttgttgttag ctactggcaa gttggttttc tatatgaatt ttgtgatttt
     2521 atagttctct taaaggatgt tcctcagatt tttaaaagga ttggtcagtc tacaaacttc
     2581 tttgtctggt atgggaagtc tttccatctt gggatttctc cagtgacttt gatcagcgct
     2641 ttgtaatttc cacagtagag gtctttctga acctcagctg aatttactcc aaggtatttt
     2701 acttgtaact gctgtgaatg gatttgcctt tacagctgcc tttacgaagt tttgttagaa
     2761 gcaggataaa actgagcctg catgtagtca tttttggctt gacctatctg gacctaagat
     2821 ctcactgcaa acttgtgagc tctaaaataa gaagcttaag aacaaagcaa gacatccctg
     2881 tcctcagcaa ggaagacttg tgcttaccat aaagggacag acacgtccta atgcaggtaa
     2941 cagcgactta aatatttctc accaatacag catcctcttc tagaaaaaga aatatatgca
     3001 tgcatgcatg catgcatgta tatatgtatg tgtatatatt tgaatactat ttgttcataa
     3061 aaagaataaa atcatgccgc ccatcatctg tggcaaatgg atctgaaggg catcatgtta
     3121 agcaaaataa aagccagaca tagcaaa
//
