LOCUS       NM_026336               1319 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus RIKEN cDNA 2310057J18 gene (2310057J18Rik), mRNA.
ACCESSION   NM_026336
VERSION     NM_026336.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK010222.1, AK009921.1 and AK008932.1.
            
            On Aug 15, 2009 this sequence version replaced NM_026336.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK010222.1, AK009961.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849378 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AK010222.1         1-3
            4-8                 AK009921.1         2-6
            9-1241              AK010222.1         9-1241
            1242-1319           AK008932.1         1364-1441
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 16.46 cM"
     gene            1..1319
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /note="RIKEN cDNA 2310057J18 gene"
                     /db_xref="GeneID:67719"
                     /db_xref="MGI:MGI:1914969"
     exon            1..315
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1028
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /note="UPF0762 protein C6orf58 homolog; protein LEG1
                     homolog"
                     /codon_start=1
                     /product="protein LEG1 homolog precursor"
                     /protein_id="NP_080612.1"
                     /db_xref="CCDS:CCDS23759.1"
                     /db_xref="GeneID:67719"
                     /db_xref="MGI:MGI:1914969"
                     /translation="MAVLASWVWVLAGCFCAAVAEVSDSSDPYPPLWEDSPEQLSDYM
                     MEDGNYIINPWVYTDRMGMYRILLEETAMYFAKYGPENEQNLLWGLPLQFGWQYQSGR
                     LADPTGMTDCGNELNESLCVSVDSWWADINYYLSVIPFLAAVDSGITGISPNQITILP
                     PPKDQMRFCYNVSDCQSAVPGTMNRWRDFFQYMQLNSSDFDGLLNYLWEAHASSLDYP
                     TSAFGDRYNFYSEKEANFEENWAIAVNYLAAARLPTTQNRTYSFQRGLPPRVLVDTDI
                     APFIPDFTPLQNEVLVSLKLLGDTDRNSGSLSLTLWENLMSTKLARTLFLKAFEEFLA
                     TSS"
     exon            316..402
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /inference="alignment:Splign:2.1.0"
     exon            403..587
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /inference="alignment:Splign:2.1.0"
     exon            588..688
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /inference="alignment:Splign:2.1.0"
     exon            689..927
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1319
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1221..1226
                     /regulatory_class="polyA_signal_sequence"
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /note="hexamer: ATTAAA"
     polyA_site      1243
                     /gene="2310057J18Rik"
                     /gene_synonym="2210414P04Rik; Leg1"
                     /note="major polyA site"
ORIGIN      
        1 cccagtttgg caccatggct gtcctggctt cctgggtctg ggtactggct ggctgcttct
       61 gtgcagctgt agcagaggtt tctgatagct cagatccata tcctccttta tgggaagaca
      121 gccctgagca gttgagtgac tacatgatgg aggatgggaa ctacataatt aatccttggg
      181 tatacactga cagaatgggg atgtacagaa tcttactgga agagacagct atgtattttg
      241 caaaatatgg tccagaaaat gaacaaaatc ttctttgggg attgcctctg cagtttggct
      301 ggcagtatca atcaggtaga ttagctgatc ccactggaat gacagactgt ggcaatgaac
      361 ttaatgagag tctgtgtgtc tctgtggaca gttggtgggc agatataaat tattatctga
      421 gtgtgatacc tttccttgct gctgttgatt ctggaataac aggaatatca ccaaaccaaa
      481 tcacgatcct gcctccaccc aaggatcaga tgaggttttg ttataatgtt tctgactgtc
      541 aatctgccgt ccctggaaca atgaacagat ggagagactt ttttcagtac atgcagttga
      601 attccagtga ctttgatggc cttcttaact acttatggga agcccatgcc tcatccttgg
      661 actatcccac cagtgccttt ggagacagat ataatttcta ttctgaaaaa gaagcaaatt
      721 ttgaggaaaa ctgggctatt gctgtgaact atttagcggc agcccgtctt cctacaaccc
      781 aaaatagaac atacagcttt cagaggggcc tgcccccacg agtccttgtt gatactgaca
      841 tagccccttt tattcctgac ttcactcctc ttcagaacga agtcctggta tcactcaaac
      901 ttcttggtga tactgacaga aattcaggat cattatcttt gactctatgg gaaaatctca
      961 tgagtacaaa acttgcaaga acattatttc taaaagcctt tgaagagttc ctggcaacat
     1021 cttcttaaga cataaaattt ttggtgaatt gttaacaact gcctttgacc acaagcaatt
     1081 gaaaacaatg gctatgatta caatcattta gatctatgat ttgggctttt ttcctagtgt
     1141 tcatttttca gggttttctg catttattcc tagtaatgat tttaaatact ggtcaatgac
     1201 aacgaagcca gaaagaaatc attaaactga tattaagctt ctatagctgc tttgattttt
     1261 gaatatagaa cgatatatct tactgatgtt taaccaaaat ccagaacatt atgaaatcc
//
