LOCUS       NM_003461               2228 bp    mRNA    linear   PRI 07-MAY-2024
DEFINITION  Homo sapiens zyxin (ZYX), transcript variant 1, mRNA.
ACCESSION   NM_003461
VERSION     NM_003461.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Kliewe,F., Siegerist,F., Hammer,E., Al-Hasani,J., Amling,T.R.J.,
            Hollemann,J.Z.E., Schindler,M., Drenic,V., Simm,S., Amann,K.,
            Daniel,C., Lindenmeyer,M., Hecker,M., Volker,U. and Endlich,N.
  TITLE     Zyxin is important for the stability and function of podocytes,
            especially during mechanical stretch
  JOURNAL   Commun Biol 7 (1), 446 (2024)
   PUBMED   38605154
  REMARK    GeneRIF: Zyxin is important for the stability and function of
            podocytes, especially during mechanical stretch.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Cai,T., Bai,J., Tan,P., Huang,Z., Liu,C., Wu,Z., Cheng,Y., Li,T.,
            Chen,Y., Ruan,J., Gao,L., DU,Y. and Fu,W.
  TITLE     Zyxin promotes hepatocellular carcinoma progression via the
            activation of AKT/mTOR signaling pathway
  JOURNAL   Oncol Res 31 (5), 805-817 (2023)
   PUBMED   37547758
  REMARK    GeneRIF: Zyxin promotes hepatocellular carcinoma progression via
            the activation of AKT/mTOR signaling pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2228)
  AUTHORS   Huang,Y., Zhao,H., Zhang,Y., Tang,Y., Shi,X., Jiang,S., Pu,W.,
            Liu,J., Ma,Y., Lin,J., Lin,J., Wu,W., Gong,Y., Wang,J. and Liu,Q.
  TITLE     Enhancement of Zyxin Promotes Skin Fibrosis by Regulating
            FAK/PI3K/AKT and TGF-beta Signaling Pathways via Integrins
  JOURNAL   Int J Biol Sci 19 (8), 2394-2408 (2023)
   PUBMED   37215989
  REMARK    GeneRIF: Enhancement of Zyxin Promotes Skin Fibrosis by Regulating
            FAK/PI3K/AKT and TGF-beta Signaling Pathways via Integrins.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2228)
  AUTHORS   Partynska,A., Gomulkiewicz,A., Piotrowska,A., Grzegrzolka,J.,
            Rzechonek,A., Ratajczak-Wielgomas,K., Podhorska-Okolow,M. and
            Dziegiel,P.
  TITLE     Expression of Zyxin in Non-Small Cell Lung Cancer-A Preliminary
            Study
  JOURNAL   Biomolecules 12 (6), 827 (2022)
   PUBMED   35740950
  REMARK    GeneRIF: Expression of Zyxin in Non-Small Cell Lung Cancer-A
            Preliminary Study.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2228)
  AUTHORS   Yan,R., Ge,X., Pang,N., Ye,H., Yuan,L., Cheng,B., Zhou,K., Yang,M.,
            Sun,Y., Zhang,S., Ding,Z., Luo,J., Ruan,C. and Dai,K.
  TITLE     Essential role of zyxin in platelet biogenesis and glycoprotein
            Ib-IX surface expression
  JOURNAL   Cell Death Dis 12 (11), 955 (2021)
   PUBMED   34657146
  REMARK    GeneRIF: Essential role of zyxin in platelet biogenesis and
            glycoprotein Ib-IX surface expression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2228)
  AUTHORS   Macalma,T., Otte,J., Hensler,M.E., Bockholt,S.M., Louis,H.A.,
            Kalff-Suske,M., Grzeschik,K.H., von der Ahe,D. and Beckerle,M.C.
  TITLE     Molecular characterization of human zyxin
  JOURNAL   J Biol Chem 271 (49), 31470-31478 (1996)
   PUBMED   8940160
REFERENCE   7  (bases 1 to 2228)
  AUTHORS   Zumbrunn,J. and Trueb,B.
  TITLE     A zyxin-related protein whose synthesis is reduced in virally
            transformed fibroblasts
  JOURNAL   Eur J Biochem 241 (2), 657-663 (1996)
   PUBMED   8917469
REFERENCE   8  (bases 1 to 2228)
  AUTHORS   Hobert,O., Schilling,J.W., Beckerle,M.C., Ullrich,A. and Jallal,B.
  TITLE     SH3 domain-dependent interaction of the proto-oncogene product Vav
            with the focal contact protein zyxin
  JOURNAL   Oncogene 12 (7), 1577-1581 (1996)
   PUBMED   8622875
REFERENCE   9  (bases 1 to 2228)
  AUTHORS   Reinhard,M., Jouvenal,K., Tripier,D. and Walter,U.
  TITLE     Identification, purification, and characterization of a
            zyxin-related protein that binds the focal adhesion and
            microfilament protein VASP (vasodilator-stimulated phosphoprotein)
  JOURNAL   Proc Natl Acad Sci U S A 92 (17), 7956-7960 (1995)
   PUBMED   7644520
REFERENCE   10 (bases 1 to 2228)
  AUTHORS   Wang,L.F., Miao,S.Y., Zong,S.D., Bai,Y. and Koide,S.S.
  TITLE     Gene encoding a mammalian epididymal protein
  JOURNAL   Biochem Mol Biol Int 34 (6), 1131-1136 (1994)
   PUBMED   7696985
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC008743.2.
            
            On Nov 22, 2018 this sequence version replaced NM_003461.4.
            
            Summary: Focal adhesions are actin-rich structures that enable
            cells to adhere to the extracellular matrix and at which protein
            complexes involved in signal transduction assemble. Zyxin is a
            zinc-binding phosphoprotein that concentrates at focal adhesions
            and along the actin cytoskeleton. Zyxin has an N-terminal
            proline-rich domain and three LIM domains in its C-terminal half.
            The proline-rich domain may interact with SH3 domains of proteins
            involved in signal transduction pathways while the LIM domains are
            likely involved in protein-protein binding. Zyxin may function as a
            messenger in the signal transduction pathway that mediates
            adhesion-stimulated changes in gene expression and may modulate the
            cytoskeletal organization of actin bundles. Alternative splicing
            results in multiple transcript variants that encode the same
            isoform. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008743.2, X95735.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000322764.10/ ENSP00000324422.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2228              BC008743.2         43-2270
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q34"
     gene            1..2228
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /note="zyxin"
                     /db_xref="GeneID:7791"
                     /db_xref="HGNC:HGNC:13200"
                     /db_xref="MIM:602002"
     exon            1..65
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     exon            66..288
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..1799
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     zyxin-2"
                     /codon_start=1
                     /product="zyxin isoform 1"
                     /protein_id="NP_003452.1"
                     /db_xref="CCDS:CCDS5883.1"
                     /db_xref="GeneID:7791"
                     /db_xref="HGNC:HGNC:13200"
                     /db_xref="MIM:602002"
                     /translation="MAAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDS
                     EPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEE
                     SFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTK
                     NDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQS
                     QTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPV
                     ASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPP
                     PAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLA
                     RAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEP
                     ITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCS
                     EPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCH
                     TARAQT"
     exon            289..488
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     exon            489..581
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     exon            582..1103
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1224
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1394
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1573
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1694
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..2228
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2202..2207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /note="hexamer: AATAAA"
     polyA_site      2228
                     /gene="ZYX"
                     /gene_synonym="ESP-2; HED-2"
                     /note="major polyA site"
ORIGIN      
        1 gcagagtctg cggacccggc gccgaggcgg ccacccgaga cgcggcgcgc acgctccggc
       61 ctgcgcagcc cggcccggcc atggcggccc cccgcccgtc tcccgcgatc tccgtttcgg
      121 tctcggctcc ggctttttac gccccgcaga agaagttcgg ccctgtggtg gccccaaagc
      181 ccaaagtgaa tcccttccgg cccggggaca gcgagcctcc cccggcaccc ggggcccagc
      241 gcgcacagat gggccgggtg ggcgagattc ccccgccgcc cccggaagac tttcccctgc
      301 ctccacctcc ccttgctggg gatggcgacg atgcagaggg tgctctggga ggtgccttcc
      361 cgccgccccc tcccccgatc gaggaatcat ttccccctgc gcctctggag gaggagatct
      421 tcccttcccc gccgcctcct ccggaggagg agggagggcc tgaggccccc ataccgcccc
      481 caccacagcc cagggagaag gtgagcagta ttgatttgga gatcgactct ctgtcctcac
      541 tgctggatga catgaccaag aatgatcctt tcaaagcccg ggtgtcatct ggatatgtgc
      601 ccccaccagt ggccactcca ttcagttcca agtccagtac caagcctgca gccgggggca
      661 cagcacccct gcctccttgg aagtcccctt ccagctccca gcctctgccc caggttccgg
      721 ctccggctca gagccagaca cagttccatg ttcagcccca gccccagccc aagcctcagg
      781 tccaactcca tgtccagtcc cagacccagc ctgtgtcttt ggctaacacc cagccccgag
      841 ggcccccagc ctcatctccg gctccagccc ctaagttttc tccagtgact cctaagttta
      901 ctcctgtggc ttccaagttc agtcctggag ccccaggtgg atctgggtca caaccaaatc
      961 aaaaattggg gcaccccgaa gctctttctg ctggcacagg ctcccctcaa cctcccagct
     1021 tcacctatgc ccagcagagg gagaagcccc gagtgcagga gaagcagcac cccgtgcccc
     1081 caccggctca gaaccaaaac caggtgcgct cccctggggc cccagggccc ctgactctga
     1141 aggaggtgga ggagctggag cagctgaccc agcagctaat gcaggacatg gagcatcctc
     1201 agaggcagaa tgtggctgtc aacgaactct gcggccgatg ccatcaaccc ctggcccggg
     1261 cgcagccagc cgtccgcgct ctagggcagc tgttccacat cgcctgcttc acctgccacc
     1321 agtgtgcgca gcagctccag ggccagcagt tctacagtct ggagggggcg ccgtactgcg
     1381 agggctgtta cactgacacc ctggagaagt gtaacacctg cggggagccc atcactgacc
     1441 gcatgctgag ggccacgggc aaggcctatc acccgcactg cttcacctgt gtggtctgcg
     1501 cccgccccct ggagggcacc tccttcatcg tggaccaggc caaccggccc cactgtgtcc
     1561 ccgactacca caagcagtac gccccgaggt gctccgtctg ctctgagccc atcatgcctg
     1621 agcctggccg agatgagact gtgcgagtgg tcgccctgga caagaacttc cacatgaagt
     1681 gttacaagtg tgaggactgc gggaagcccc tgtcgattga ggcagatgac aatggctgct
     1741 tccccctgga cggtcacgtg ctctgtcgga agtgccacac tgctagagcc cagacctgag
     1801 tgaggacagg ccctcttcag accgcagtcc atgccccatt gtggaccacc cacactgaga
     1861 ccacctgccc ccacctcagt tattgttttg atgtctagcc cctcccattt ccaacccctc
     1921 cctagcatcc caggtgccct gacccaggac ccaacatggt ctagggatgc aggatccccg
     1981 ccctggggtc tggtcctcgc ccatcctgca gggattgccc accgtcttcc agacacccca
     2041 cctgaggggg gcaccaggtt tagtgctgct gctttcactg ctgcacccgc gccctcggcc
     2101 ggccccccga gcagcctttg tactctgctt gcggagggct gggagaccct ccaggacatt
     2161 cccaccctcc cccatgctgc caagttgtag ctatagctac aaataaaaaa aaaccttgtt
     2221 ttccagaa
//
