LOCUS       NM_004724               2873 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens zw10 kinetochore protein (ZW10), mRNA.
ACCESSION   NM_004724
VERSION     NM_004724.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Li,D., Zhao,Y.G., Li,D., Zhao,H., Huang,J., Miao,G., Feng,D.,
            Liu,P., Li,D. and Zhang,H.
  TITLE     The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact
            Formation
  JOURNAL   Cell Rep 27 (2), 343-358 (2019)
   PUBMED   30970241
REFERENCE   3  (bases 1 to 2873)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 2873)
  AUTHORS   Mosalaganti,S., Keller,J., Altenfeld,A., Winzker,M., Rombaut,P.,
            Saur,M., Petrovic,A., Wehenkel,A., Wohlgemuth,S., Muller,F.,
            Maffini,S., Bange,T., Herzog,F., Waldmann,H., Raunser,S. and
            Musacchio,A.
  TITLE     Structure of the RZZ complex and molecular basis of its interaction
            with Spindly
  JOURNAL   J Cell Biol 216 (4), 961-981 (2017)
   PUBMED   28320825
REFERENCE   5  (bases 1 to 2873)
  AUTHORS   Altenfeld,A., Wohlgemuth,S., Wehenkel,A., Vetter,I.R. and
            Musacchio,A.
  TITLE     Complex assembly, crystallization and preliminary X-ray
            crystallographic analysis of the human Rod-Zwilch-ZW10 (RZZ)
            complex
  JOURNAL   Acta Crystallogr F Struct Biol Commun 71 (Pt 4), 438-442 (2015)
   PUBMED   25849506
  REMARK    GeneRIF: The RZZ complex was purified to homogeneity and subjected
            to systemic crystallization.
REFERENCE   6  (bases 1 to 2873)
  AUTHORS   Scaerou,F., Starr,D.A., Piano,F., Papoulas,O., Karess,R.E. and
            Goldberg,M.L.
  TITLE     The ZW10 and Rough Deal checkpoint proteins function together in a
            large, evolutionarily conserved complex targeted to the kinetochore
  JOURNAL   J Cell Sci 114 (Pt 17), 3103-3114 (2001)
   PUBMED   11590237
REFERENCE   7  (bases 1 to 2873)
  AUTHORS   Chan,G.K., Jablonski,S.A., Starr,D.A., Goldberg,M.L. and Yen,T.J.
  TITLE     Human Zw10 and ROD are mitotic checkpoint proteins that bind to
            kinetochores
  JOURNAL   Nat Cell Biol 2 (12), 944-947 (2000)
   PUBMED   11146660
REFERENCE   8  (bases 1 to 2873)
  AUTHORS   Starr,D.A., Saffery,R., Li,Z., Simpson,A.E., Choo,K.H., Yen,T.J.
            and Goldberg,M.L.
  TITLE     HZwint-1, a novel human kinetochore component that interacts with
            HZW10
  JOURNAL   J Cell Sci 113 (Pt 11), 1939-1950 (2000)
   PUBMED   10806105
REFERENCE   9  (bases 1 to 2873)
  AUTHORS   Starr,D.A., Williams,B.C., Hays,T.S. and Goldberg,M.L.
  TITLE     ZW10 helps recruit dynactin and dynein to the kinetochore
  JOURNAL   J Cell Biol 142 (3), 763-774 (1998)
   PUBMED   9700164
REFERENCE   10 (bases 1 to 2873)
  AUTHORS   Starr,D.A., Williams,B.C., Li,Z., Etemad-Moghadam,B., Dawe,R.K. and
            Goldberg,M.L.
  TITLE     Conservation of the centromere/kinetochore protein ZW10
  JOURNAL   J Cell Biol 138 (6), 1289-1301 (1997)
   PUBMED   9298984
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM833775.1, U54996.1,
            BC128264.1, DB506745.2 and BQ773926.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004724.3.
            
            Summary: This gene encodes a protein that is one of many involved
            in mechanisms to ensure proper chromosome segregation during cell
            division. This protein is an essential component of the mitotic
            checkpoint, which prevents cells from prematurely exiting mitosis.
            [provided by RefSeq, Aug 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U54996.1, AK312925.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000200135.8/ ENSP00000200135.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                BM833775.1         72-81
            11-2508             U54996.1           8-2505
            2509-2785           BC128264.1         2459-2735
            2786-2871           DB506745.2         564-649
            2872-2873           BQ773926.1         21-22               c
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.2"
     gene            1..2873
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /note="zw10 kinetochore protein"
                     /db_xref="GeneID:9183"
                     /db_xref="HGNC:HGNC:13194"
                     /db_xref="MIM:603954"
     exon            1..131
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..2366
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /note="zeste white 10 homolog; ZW10 homolog,
                     centromere/kinetochore protein; ZW10, kinetochore
                     associated, homolog"
                     /codon_start=1
                     /product="centromere/kinetochore protein zw10 homolog"
                     /protein_id="NP_004715.1"
                     /db_xref="CCDS:CCDS8363.1"
                     /db_xref="GeneID:9183"
                     /db_xref="HGNC:HGNC:13194"
                     /db_xref="MIM:603954"
                     /translation="MASFVTEVLAHSGRLEKEDLGTRISRLTRRVEEIKGEVCNMISK
                     KYSEFLPSMQSAQGLITQVDKLSEDIDLLKSRIESEVRRDLHVSTGEFTDLKQQLERD
                     SVVLSLLKQLQEFSTAIEEYNCALTEKKYVTGAQRLEEAQKCLKLLKSRKCFDLKILK
                     SLSMELTIQKQNILYHLGEEWQKLIVWKFPPSKDTSSLESYLQTELHLYTEQSHKEEK
                     TPMPPISSVLLAFSVLGELHSKLKSFGQMLLKYILRPLASCPSLHAVIESQPNIVIIR
                     FESIMTNLEYPSPSEVFTKIRLVLEVLQKQLLDLPLDTDLENEKTSTVPLAEMLGDMI
                     WEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYA
                     RNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKV
                     SNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQL
                     FYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPI
                     LCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASK
                     AVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALEDISTEDGDRL
                     YSLCKTVMDEGPQVFAPLSEESKNKKYQEEVPVYVPKWMPFKELMMMLQASLQEIGDR
                     WADGKGPLAAAFSSSEVKALIRALFQNTERRAAALAKIK"
     exon            132..266
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            267..368
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            369..446
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            447..606
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            607..759
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            760..951
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1115
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1298
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1537
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1609
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1779
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1910
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2042
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2245
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     exon            2246..2873
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2853..2858
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /note="hexamer: AATAAA"
     polyA_site      2873
                     /gene="ZW10"
                     /gene_synonym="HZW10; KNTC1AP"
                     /note="major polyA site"
ORIGIN      
        1 agtcagcgtt ggttcccgtc ttggccatgg cctcgttcgt gacagaagtt ttggcacact
       61 ccgggaggct ggaaaaggag gatctgggga cccggatcag ccgcctgacc cggcgggtgg
      121 aggagatcaa gggtgaggtg tgcaatatga ttagcaagaa gtacagtgaa ttcctgccta
      181 gcatgcagag cgcgcagggc ctgattaccc aggtggataa gctatctgaa gacattgacc
      241 tgctgaaatc caggatagag agtgaggtcc gccgggatct tcacgtatca accggtgaat
      301 ttacagactt aaagcagcag ttggaaagag actcagttgt cctaagtttg cttaaacagt
      361 tgcaggagtt ttccactgct attgaagaat ataattgtgc attaacagag aagaagtatg
      421 tcactggtgc tcagcgtctg gaagaggcac agaaatgctt gaagttatta aaatccagaa
      481 aatgctttga tttaaaaata ttgaaatctc tcagcatgga gctcacaata cagaaacaga
      541 acatacttta tcaccttgga gaagagtggc agaagctgat tgtatggaag ttcccaccat
      601 caaaagatac cagcagtttg gaatcttacc tacaaactga acttcattta tacactgaac
      661 aatcgcacaa agaggagaag acccctatgc cacccatcag ttctgtcctc ttggcatttt
      721 ctgttcttgg agaactacac agcaagctta aatcatttgg tcagatgctg ctgaagtata
      781 tccttaggcc gctggcatct tgcccatccc ttcatgctgt gatagaaagc cagcctaaca
      841 tagttattat tcgttttgaa tctataatga ctaacttgga atatccatca ccatctgaag
      901 tttttacaaa gatcagactg gtactagaag tgctccagaa acagcttcta gatttgccac
      961 ttgacactga cctggaaaat gaaaaaacat ctactgtccc attggctgag atgcttggag
     1021 acatgatctg ggaggacttg tctgagtgcc tcatcaaaaa ctgtttggtt tattcgattc
     1081 caacaaatag cagcaaatta cagcaatatg aagagatcat acagtccact gaagaatttg
     1141 aaaatgccct aaaggaaatg agatttttaa aaggagatac tacagatttg ctgaaatacg
     1201 ctcgtaacat caattctcat tttgcaaaca aaaagtgcca ggatgtgatt gtggcagcca
     1261 gaaatctaat gacctcagaa attcataaca ctgtgaagat tattcctgat tctaagataa
     1321 atgtgccaga gttacccact cctgatgagg ataacaaact ggaagtacag aaagtatcca
     1381 atactcagta ccacgaagtg atgaatttag agcctgaaaa tacattggac caacattcct
     1441 tttccttgcc cacatgccgt atcagtgagt ctgtgaagaa attaatggaa ctcgcctatc
     1501 agactttact agaggcaaca accagtagtg atcaatgtgc tgttcaactt ttctactcag
     1561 tgaggaatat cttccatttg ttccatgatg ttgtaccaac atatcacaag gagaaccttc
     1621 aaaaacttcc ccagttggct gctattcatc acaacaactg tatgtacatt gctcaccact
     1681 tgctgaccct cgggcatcag ttcagattgc gtcttgcccc cattctttgt gatggcactg
     1741 ctacttttgt ggatcttgta cctggcttca ggagacttgg gacagaatgc tttttggccc
     1801 aaatgcgggc acagaaaggt gaacttctgg aaagattatc aagtgctagg aacttttcaa
     1861 atatggacga tgaagagaat tattctgcag caagtaaagc agtccggcag gtactgcacc
     1921 aactaaagag acttggaatt gtgtggcagg atgtcctgcc agtgaatata tattgcaagg
     1981 ctatggggac tttactcaat acagcaattt ctgaggtcat tggcaaaatt actgccctag
     2041 aggacatatc tactgaagat ggtgataggt tatattcctt atgcaaaaca gtgatggatg
     2101 aaggacccca agtatttgca cctttatctg aagaaagcaa gaacaagaaa tatcaagaag
     2161 aggttccagt ctatgtgcca aaatggatgc cattcaagga attgatgatg atgctacaag
     2221 ccagcttgca agaaattggg gatcggtggg cagatggaaa aggacccctg gcagctgcgt
     2281 tctcttccag tgaagtaaaa gctttaattc gtgccttgtt tcagaacaca gaaagaagag
     2341 cagctgccct tgctaaaatt aaatagctcc atcttcttaa gaaagctatg tcttgaatat
     2401 gtggattctt cccttggcat aattactccc ttaaagactt ctttgaatcg cccattggtt
     2461 ttggtgaacc agtacatctt ggaagtttga ctttacagaa gaacgtctta cctcctggcc
     2521 tgtacgaggc tttgtttaag aactgtttat taagataaat tgtcaagtaa agcacctcaa
     2581 ttcattgact ttctagccat cttcctttga ttagctaaca aactgtcagg cagcattatt
     2641 tcatgctgct tccagagccc tctgggagct atatacattg taaatgcagg ccctagcttt
     2701 ggaacgagga attgggagat tccaggagtc agggtagaga atttctgagc aaatcggaga
     2761 tattttaggg gtgtggagga ggggaaggga ggaatgggcc accatatttg gccttacagg
     2821 aattaaggag acttcctgta atatttcttt ccaataaata ttgcttttta caa
//
