LOCUS       NP_659499                578 aa            linear   PRI 05-APR-2024
DEFINITION  zinc finger-containing ubiquitin peptidase 1 isoform 1 [Homo
            sapiens].
ACCESSION   NP_659499
VERSION     NP_659499.2
DBSOURCE    REFSEQ: accession NM_145062.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 578)
  AUTHORS   Ajadi,M.B., Soremekun,O.S., Adewumi,A.T., Kumalo,H.M. and
            Soliman,M.E.S.
  TITLE     Functional Analysis of Single Nucleotide Polymorphism in ZUFSP
            Protein and Implication in Pathogenesis
  JOURNAL   Protein J 40 (1), 28-40 (2021)
   PUBMED   33512633
  REMARK    GeneRIF: Functional Analysis of Single Nucleotide Polymorphism in
            ZUFSP Protein and Implication in Pathogenesis.
REFERENCE   2  (residues 1 to 578)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (residues 1 to 578)
  AUTHORS   Haahr,P., Borgermann,N., Guo,X., Typas,D., Achuthankutty,D.,
            Hoffmann,S., Shearer,R., Sixma,T.K. and Mailand,N.
  TITLE     ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote
            Genome Stability
  JOURNAL   Mol Cell 70 (1), 165-174 (2018)
   PUBMED   29576528
  REMARK    GeneRIF: ZUFSP is recruited to and regulates K63-Ub conjugates at
            genotoxic stress sites, promoting chromosome stability upon
            replication stress in a manner dependent on its catalytic activity.
REFERENCE   4  (residues 1 to 578)
  AUTHORS   Kwasna,D., Abdul Rehman,S.A., Natarajan,J., Matthews,S., Madden,R.,
            De Cesare,V., Weidlich,S., Virdee,S., Ahel,I., Gibbs-Seymour,I. and
            Kulathu,Y.
  TITLE     Discovery and Characterization of ZUFSP/ZUP1, a Distinct
            Deubiquitinase Class Important for Genome Stability
  JOURNAL   Mol Cell 70 (1), 150-164 (2018)
   PUBMED   29576527
  REMARK    GeneRIF: Identify two ubiquitin binding domains in ZUFSP: a ZHA
            (ZUFSP helical arm) that binds to the distal ubiquitin and an
            atypical UBZ domain in ZUFSP that binds to polyubiquitin.
            Importantly, both domains are essential for ZUFSP to selectively
            cleave K63-linked polyubiquitin.
REFERENCE   5  (residues 1 to 578)
  AUTHORS   Hermanns,T., Pichlo,C., Woiwode,I., Klopffleisch,K., Witting,K.F.,
            Ovaa,H., Baumann,U. and Hofmann,K.
  TITLE     A family of unconventional deubiquitinases with modular chain
            specificity determinants
  JOURNAL   Nat Commun 9 (1), 799 (2018)
   PUBMED   29476094
  REMARK    GeneRIF: ZUFSP,the singular human member of this family, which
            contains multiple ubiquitin-binding domains responsible for the
            specific action on K63-linked chains.
            GeneRIF: ZUFSP (ZUP1) is a K63-specific deubiquitinating enzyme
            that is distantly related to proteases for the ubiquitin-like
            modifiers Ufm1 and Atg8. ZUFSP does not have any activity on these
            modifiers. ZUFSP specifically cleaves long K63-linked
            poly-ubiquitin chains in an ''endo'' mode, i.e. cleavage takes
            place in the middle of a chain rather than by removing the terminal
            ubiquitin from a chain.
            Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC016879.1.
            
            On Mar 30, 2010 this sequence version replaced NP_659499.1.
            
            Summary: This gene encodes a protein containing zinc finger motifs
            and a cysteine peptidase domain. The encoded protein functions as a
            K63-specific de-ubiquitinating enzyme that specifically cleaves
            long K63-linked polyubiquitin chains in the middle of a chain (i.e.
            'endo cleavage) rather than by removing the terminal ubiquitin from
            a chain. This enzyme is thought to be involved in the regulation of
            DNA repair by cleaving K63-linked ubiquitin chains at repair foci.
            This protein is related to proteases for the ubiquitin-like
            modifiers Ufm1 (ubiquitin fold modifier 1) and Atg8/Gabarapl2, but
            does not have any activity on these modifiers. [provided by RefSeq,
            Mar 2018].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016879.1, AK054582.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368576.8/ ENSP00000357565.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q22.1"
     Protein         1..578
                     /product="zinc finger-containing ubiquitin peptidase 1
                     isoform 1"
                     /EC_number="3.4.19.12"
                     /note="zinc finger with UFM1-specific peptidase domain
                     protein; lys-63-specific deubiquitinase ZUFSP; ubiquitin
                     carboxyl-terminal hydrolase ZUFSP"
                     /calculated_mol_wt=65828
     Region          226..248
                     /region_name="MIU. /evidence=ECO:0000305|PubMed:29476094,
                     ECO:0000305|PubMed:29576527"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96AP4.1)"
     Region          249..274
                     /region_name="zUBD/ZHA.
                     /evidence=ECO:0000305|PubMed:29476094,
                     ECO:0000305|PubMed:29576527"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96AP4.1)"
     Site            262
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0007744|PubMed:19608861; propagated from
                     UniProtKB/Swiss-Prot (Q96AP4.1)"
     Region          336..551
                     /region_name="Peptidase_C78"
                     /note="Peptidase family C78; pfam07910"
                     /db_xref="CDD:400318"
     Site            487
                     /site_type="other"
                     /note="Involved in the stabilization of negative charge on
                     the oxyanion by the formation of the oxyanion hole.
                     /evidence=ECO:0000305|PubMed:29576527; propagated from
                     UniProtKB/Swiss-Prot (Q96AP4.1)"
     CDS             1..578
                     /gene="ZUP1"
                     /gene_synonym="C6orf113; DUB; ZUFSP"
                     /coded_by="NM_145062.3:217..1953"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS5110.1"
                     /db_xref="GeneID:221302"
                     /db_xref="HGNC:HGNC:21224"
                     /db_xref="MIM:620543"
ORIGIN      
        1 mlscnicget vtsepdmkah livhmeseii cpfcklsgvn ydemcfhiet ahfeqntler
       61 nferintvqy gtsdnkkdnt lqcgmevnss ilsgcasnhp knsaqnltkd stlkhegfys
      121 enltesrkfl ksrekqsslt eikgsvyett ysppecpfcg kieehsedme thvktkhanl
      181 ldipledcdq plydcpmcgl ictnyhilqe hvdlhleens fqqgmdrvqc sgdlqlahql
      241 qqeedrkrrs eesrqeieef qklqrqygld nsggykqqql rnmeievnrg rmppsefhrr
      301 kadmmeslal gfddgktkts giiealhryy qnaatdvrrv wlssvvdhfh sslgdkgwgc
      361 gyrnfqmlls sllqndaynd clkgmlipci pkiqsmieda wkegfdpqga sqlnnrlqgt
      421 kawigacevy illtslrvkc hivdfhkstg plgthprlfe wilnyysseg egspkvvcts
      481 kppiylqhqg hsrtvigiee kknrtlclli ldpgcpsrem qkllkqdiea sslkqlrksm
      541 gnlkhkqyqi lavegalsle eklarrqasq vftaekip
//
