LOCUS       NM_021186               1928 bp    mRNA    linear   PRI 16-FEB-2024
DEFINITION  Homo sapiens zona pellucida glycoprotein 4 (ZP4), mRNA.
ACCESSION   NM_021186
VERSION     NM_021186.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Wei,X., Li,Y., Liu,Q., Liu,W., Yan,X., Zhu,X., Zhou,D., Tian,Y.,
            Zhang,F., Li,N. and Lu,Z.
  TITLE     Mutations in ZP4 are associated with abnormal zona pellucida and
            female infertility
  JOURNAL   J Clin Pathol 75 (3), 201-204 (2022)
   PUBMED   33461974
  REMARK    GeneRIF: Mutations in ZP4 are associated with abnormal zona
            pellucida and female infertility.
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Litscher,E.S. and Wassarman,P.M.
  TITLE     Zona Pellucida Proteins, Fibrils, and Matrix
  JOURNAL   Annu Rev Biochem 89, 695-715 (2020)
   PUBMED   32569527
  REMARK    GeneRIF: Zona Pellucida Proteins, Fibrils, and Matrix.
            Review article
REFERENCE   3  (bases 1 to 1928)
  AUTHORS   Dai,C., Hu,L., Gong,F., Tan,Y., Cai,S., Zhang,S., Dai,J., Lu,C.,
            Chen,J., Chen,Y., Lu,G., Du,J. and Lin,G.
  TITLE     ZP2 pathogenic variants cause in vitro fertilization failure and
            female infertility
  JOURNAL   Genet Med 21 (2), 431-440 (2019)
   PUBMED   29895852
REFERENCE   4  (bases 1 to 1928)
  AUTHORS   Tan,Y., Zhang,D., Gong,B., Zhang,X., Jiang,L. and Lin,Y.
  TITLE     [Association of ZP4 gene polymorphism with primary open-angle
            glaucoma in an ethnic Chinese population from Sichuan province]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 35 (5), 737-740 (2018)
   PUBMED   30298508
  REMARK    GeneRIF: ZP4 gene SNPs rs547984, rs540782, rs693421, rs2499601 are
            not associated with open-angle glaucoma among ethnic Hans from
            Sichuan.
REFERENCE   5  (bases 1 to 1928)
  AUTHORS   Louros,N.N., Chrysina,E.D., Baltatzis,G.E., Patsouris,E.S.,
            Hamodrakas,S.J. and Iconomidou,V.A.
  TITLE     A common 'aggregation-prone' interface possibly participates in the
            self-assembly of human zona pellucida proteins
  JOURNAL   FEBS Lett 590 (5), 619-630 (2016)
   PUBMED   26879157
REFERENCE   6  (bases 1 to 1928)
  AUTHORS   Kiefer,S.M. and Saling,P.
  TITLE     Proteolytic processing of human zona pellucida proteins
  JOURNAL   Biol Reprod 66 (2), 407-414 (2002)
   PUBMED   11804956
REFERENCE   7  (bases 1 to 1928)
  AUTHORS   Eberspaecher,U., Becker,A., Bringmann,P., van der Merwe,L. and
            Donner,P.
  TITLE     Immunohistochemical localization of zona pellucida proteins ZPA,
            ZPB and ZPC in human, cynomolgus monkey and mouse ovaries
  JOURNAL   Cell Tissue Res 303 (2), 277-287 (2001)
   PUBMED   11291774
REFERENCE   8  (bases 1 to 1928)
  AUTHORS   Gonzalez,P., Epstein,D.L. and Borras,T.
  TITLE     Characterization of gene expression in human trabecular meshwork
            using single-pass sequencing of 1060 clones
  JOURNAL   Invest Ophthalmol Vis Sci 41 (12), 3678-3693 (2000)
   PUBMED   11053263
REFERENCE   9  (bases 1 to 1928)
  AUTHORS   Rankin,T. and Dean,J.
  TITLE     The zona pellucida: using molecular genetics to study the mammalian
            egg coat
  JOURNAL   Rev Reprod 5 (2), 114-121 (2000)
   PUBMED   10864856
  REMARK    Review article
REFERENCE   10 (bases 1 to 1928)
  AUTHORS   Harris,J.D., Hibler,D.W., Fontenot,G.K., Hsu,K.T., Yurewicz,E.C.
            and Sacco,A.G.
  TITLE     Cloning and characterization of zona pellucida genes and cDNAs from
            a variety of mammalian species: the ZPA, ZPB and ZPC gene families
  JOURNAL   DNA Seq 4 (6), 361-393 (1994)
   PUBMED   7841460
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK314151.1 and DV080723.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 13, 2020 this sequence version replaced NM_021186.4.
            
            Summary: The zona pellucida is an extracellular matrix that
            surrounds the oocyte and early embryo. It is composed primarily of
            three or four glycoproteins with various functions during
            fertilization and preimplantation development. The nascent protein
            contains a N-terminal signal peptide sequence, a conserved ZP
            domain, a consensus furin cleavage site, and a C-terminal
            transmembrane domain. It is hypothesized that furin cleavage
            results in release of the mature protein from the plasma membrane
            for subsequent incorporation into the zona pellucida matrix.
            However, the requirement for furin cleavage in this process remains
            controversial based on mouse studies. Previously, this gene has
            been referred to as ZP1 or ZPB and thought to have similar
            functions as mouse Zp1. However, a human gene with higher
            similarity and chromosomal synteny to mouse Zp1 has been assigned
            the symbol ZP1 and this gene has been assigned the symbol ZP4.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK314151.1, BC069521.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366570.5/ ENSP00000355529.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1910              AK314151.1         1-1910
            1911-1928           DV080723.1         574-591
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q43"
     gene            1..1928
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /note="zona pellucida glycoprotein 4"
                     /db_xref="GeneID:57829"
                     /db_xref="HGNC:HGNC:15770"
                     /db_xref="MIM:613514"
     exon            1..462
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             288..1910
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /note="zona pellucida sperm-binding protein 4; zona
                     pellucida protein B"
                     /codon_start=1
                     /product="zona pellucida sperm-binding protein 4
                     preproprotein"
                     /protein_id="NP_067009.1"
                     /db_xref="CCDS:CCDS1615.1"
                     /db_xref="GeneID:57829"
                     /db_xref="HGNC:HGNC:15770"
                     /db_xref="MIM:613514"
                     /translation="MWLLRCVLLCVSLSLAVSGQHKPEAPDYSSVLHCGPWSFQFAVN
                     LNQEATSPPVLIAWDNQGLLHELQNDSDCGTWIRKGPGSSVVLEATYSSCYVTEWDSH
                     YIMPVGVEGAGAAEHKVVTERKLLKCPMDLLARDAPDTDWCDSIPARDRLPCAPSPIS
                     RGDCEGLGCCYSSEEVNSCYYGNTVTLHCTREGHFSIAVSRNVTSPPLLLDSVRLALR
                     NDSACNPVMATQAFVLFQFPFTSCGTTRQITGDRAVYENELVATRDVKNGSRGSVTRD
                     SIFRLHVSCSYSVSSNSLPINVQVFTLPPPFPETQPGPLTLELQIAKDKNYGSYYGVG
                     DYPVVKLLRDPIYVEVSILHRTDPYLGLLLQQCWATPSTDPLSQPQWPILVKGCPYIG
                     DNYQTQLIPVQKALDLPFPSHHQRFSIFTFSFVNPTVEKQALRGPVHLHCSVSVCQPA
                     ETPSCVVTCPDLSRRRNFDNSSQNTTASVSSKGPMILLQATKDPPEKLRVPVDSKVLW
                     VAGLSGTLILGALLVSYLAVKKQKSCPDQMCQ"
     exon            463..584
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            585..687
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            688..840
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            841..1028
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1126
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1257
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1447
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1598
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1677
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1782
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1928
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1906..1911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /note="hexamer: AATAAA"
     polyA_site      1928
                     /gene="ZP4"
                     /gene_synonym="ZBP; Zp-4; ZP1; ZP1B; ZPB; ZPB2"
                     /note="major polyA site"
ORIGIN      
        1 agctttcttt ccttcctttc tgttagctaa aagttactac atttttcttg ggctctgaag
       61 tctgcctgga ttgtggtatt taatgtgagg cagctgcaga ctgtgcccca aagcaaatga
      121 ggctagagga attccccttt ctgaaggcat cccacctgag tgtcacaaag atgtgcagct
      181 gaaaacaagc ctgacttctg tatataaagg aatctgggca ggcagaccct cggctcttgg
      241 gagaggaggc ggagagtctg cagccggcag gaactcctgg tagcattatg tggctgctgc
      301 ggtgcgtttt gctgtgtgtt tcattatctc ttgctgtgag tggccagcat aagcctgagg
      361 caccagatta ttccagtgtg ctccactgtg ggccgtggag cttccagttt gctgtaaacc
      421 tcaaccagga ggcaacgtct cctcctgtac taatagcttg ggacaaccaa gggctgctgc
      481 acgagctgca gaatgactcc gactgtggca cctggataag aaaaggtcca ggcagctccg
      541 tggtgttgga ggcaacctat agcagctgct atgtcactga gtgggactcc cactacatca
      601 tgccagttgg agttgaagga gcaggcgcgg ctgaacacaa ggtggttaca gagaggaagc
      661 tgctcaagtg tcctatggat cttctagccc gagatgctcc agatactgac tggtgtgact
      721 ccatcccagc acgggacaga ctgccatgtg caccttcacc catctctcga ggagactgtg
      781 aagggctagg ctgttgttat agctctgaag aggtgaattc ctgctactat ggaaacactg
      841 tgaccttgca ttgtacccga gagggccatt tctctattgc tgtgtctcgg aacgtgacct
      901 cgccaccact gctcttggat tctgtgcgct tggcccttag gaatgacagt gcgtgtaacc
      961 ctgtgatggc aacacaagct tttgttctgt tccagtttcc atttacttcc tgtggcacca
     1021 caagacagat cactggagac cgagcagtat atgaaaatga actggtggca actagggatg
     1081 tgaaaaatgg gagccgtggc tctgtcactc gtgacagcat cttcaggctc catgtcagct
     1141 gcagctactc agtaagtagc aactctctcc caatcaatgt ccaggttttc actctcccac
     1201 caccctttcc tgagacccag cctggacccc tcactctgga acttcagatt gccaaagata
     1261 aaaactatgg ctcttactac ggtgttggtg actacccagt ggtgaagttg cttcgggatc
     1321 ccatttacgt ggaggtctcc atccttcaca gaacagaccc ctacctgggg ctgctcctac
     1381 aacagtgttg ggcaacaccc agcactgacc ccctgagtca gccacagtgg cccatcctgg
     1441 taaagggctg cccctacatt ggagacaact atcagaccca gctgatccct gtccagaaag
     1501 ccttggatct tccatttccc tctcaccacc agcgcttcag catcttcacc ttcagctttg
     1561 tgaaccctac agtggagaaa caggccctca ggggaccggt gcatctgcac tgcagcgtgt
     1621 cagtctgcca gcctgctgag acaccatcct gtgtggtgac ctgtcctgat ctcagtcgaa
     1681 gaagaaattt tgacaacagt tctcagaaca ctactgctag tgtttctagc aaaggcccca
     1741 tgattctact ccaagccact aaggaccctc cagaaaagct ccgtgttcct gtagactcga
     1801 aagttctgtg ggtggcaggc ctttctggga ccttaatcct tggagccttg ttagtatcct
     1861 acttggctgt caagaaacag aagagttgcc cagaccaaat gtgtcaataa aaccagagtg
     1921 tattccca
//
