LOCUS       NM_001376232            2295 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript
            variant 4, mRNA.
ACCESSION   NM_001376232
VERSION     NM_001376232.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Nishio,S., Emori,C., Wiseman,B., Fahrenkamp,D., Dioguardi,E.,
            Zamora-Caballero,S., Bokhove,M., Han,L., Stsiapanava,A.,
            Algarra,B., Lu,Y., Kodani,M., Bainbridge,R.E., Komondor,K.M.,
            Carlson,A.E., Landreh,M., de Sanctis,D., Yasumasu,S., Ikawa,M. and
            Jovine,L.
  TITLE     ZP2 cleavage blocks polyspermy by modulating the architecture of
            the egg coat
  JOURNAL   Cell 187 (6), 1440-1459 (2024)
   PUBMED   38490181
  REMARK    GeneRIF: ZP2 cleavage blocks polyspermy by modulating the
            architecture of the egg coat.
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Sun,L., Tong,K., Liu,W., Tian,Y., Liu,D., Huang,G. and Li,J.
  TITLE     Novel variants in ZP1, ZP2 and ZP3 associated with empty follicle
            syndrome and abnormal zona pellucida
  JOURNAL   Reprod Biomed Online 46 (5), 847-855 (2023)
   PUBMED   36931917
  REMARK    GeneRIF: Novel variants in ZP1, ZP2 and ZP3 associated with empty
            follicle syndrome and abnormal zona pellucida.
REFERENCE   3  (bases 1 to 2295)
  AUTHORS   Hou,M., Zhu,L., Jiang,J., Liu,Z., Li,Z., Jia,W., Hu,J., Zhou,X.,
            Zhang,D., Luo,Y., Peng,X., Xi,Q., Jin,L. and Zhang,X.
  TITLE     Novel Heterozygous Mutations in ZP2 Cause Abnormal Zona Pellucida
            and Female Infertility
  JOURNAL   Reprod Sci 29 (10), 3047-3054 (2022)
   PUBMED   35595959
  REMARK    GeneRIF: Novel Heterozygous Mutations in ZP2 Cause Abnormal Zona
            Pellucida and Female Infertility.
REFERENCE   4  (bases 1 to 2295)
  AUTHORS   Jia,W., Xi,Q., Zhu,L., Luo,Y., Li,Z., Hou,M., Zhang,D., Yang,X.,
            Hu,J., Jin,L. and Zhang,X.
  TITLE     Novel mutations in ZP2 and ZP3 cause female infertility in three
            patients
  JOURNAL   J Assist Reprod Genet 39 (5), 1205-1215 (2022)
   PUBMED   35366744
  REMARK    GeneRIF: Novel mutations in ZP2 and ZP3 cause female infertility in
            three patients.
REFERENCE   5  (bases 1 to 2295)
  AUTHORS   Wassarman,P.M. and Litscher,E.S.
  TITLE     Influence of the zona pellucida of the mouse egg on
            folliculogenesis and fertility
  JOURNAL   Int J Dev Biol 56 (10-12), 833-839 (2012)
   PUBMED   23417405
  REMARK    GeneRIF: Studies indicate that eggs from mice lacking an ortholog
            of ZP2 do not contain a zona pellucida (ZP), and the mice are
            completely infertile due to a severely reduced number of ovulated
            eggs in their oviducts.
            Review article
REFERENCE   6  (bases 1 to 2295)
  AUTHORS   Hinsch,E., Groeger,S., Oehninger,S. and Hinsch,K.D.
  TITLE     Localization and functional importance of a conserved zona
            pellucida 2 protein domain in the human and bovine ovary using
            monoclonal anti-ZP2 peptide antibodies
  JOURNAL   Theriogenology 60 (7), 1331-1344 (2003)
   PUBMED   14511786
REFERENCE   7  (bases 1 to 2295)
  AUTHORS   Mori,T., Guo,M.W., Sato,E., Baba,T., Takasaki,S. and Mori,E.
  TITLE     Molecular and immunological approaches to mammalian fertilization
  JOURNAL   J Reprod Immunol 47 (2), 139-158 (2000)
   PUBMED   10924747
  REMARK    Review article
REFERENCE   8  (bases 1 to 2295)
  AUTHORS   Rankin,T. and Dean,J.
  TITLE     The zona pellucida: using molecular genetics to study the mammalian
            egg coat
  JOURNAL   Rev Reprod 5 (2), 114-121 (2000)
   PUBMED   10864856
  REMARK    Review article
REFERENCE   9  (bases 1 to 2295)
  AUTHORS   Tsubamoto,H., Hasegawa,A., Nakata,Y., Naito,S., Yamasaki,N. and
            Koyama,K.
  TITLE     Expression of recombinant human zona pellucida protein 2 and its
            binding capacity to spermatozoa
  JOURNAL   Biol Reprod 61 (6), 1649-1654 (1999)
   PUBMED   10570015
REFERENCE   10 (bases 1 to 2295)
  AUTHORS   Liang,L.F. and Dean,J.
  TITLE     Conservation of mammalian secondary sperm receptor genes enables
            the promoter of the human gene to function in mouse oocytes
  JOURNAL   Dev Biol 156 (2), 399-408 (1993)
   PUBMED   8385033
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF001550.1 and AK313003.1.
            
            Summary: The zona pellucida is an extracellular matrix that
            surrounds the oocyte and early embryo. It is composed of three
            glycoproteins with various functions during fertilization and
            preimplantation development. The glycosylated mature peptide is one
            of the structural components of the zona pellucida and functions in
            secondary binding and penetration of acrosome-reacted spermatozoa.
            Female mice lacking this gene do not form a stable zona matrix and
            are sterile. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Feb 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M90366.1, AK313003.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968540,
                                           SAMEA1968832 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000574091.6/ ENSP00000458991.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AF001550.1         78998-78999         c
            3-2265              AK313003.1         1-2263
            2266-2295           AF001550.1         64875-64904         c
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.3-p12.2"
     gene            1..2295
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /note="zona pellucida glycoprotein 2"
                     /db_xref="GeneID:7783"
                     /db_xref="HGNC:HGNC:13188"
                     /db_xref="MIM:182888"
     exon            1..89
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..2265
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 4; zona pellucida protein A; zona pellucida
                     sperm-binding protein 2; zona pellucida glycoprotein 2
                     (sperm receptor); zona pellucida glycoprotein ZP2; zona
                     pellucida 2"
                     /codon_start=1
                     /product="zona pellucida sperm-binding protein 2 isoform 1
                     preproprotein"
                     /protein_id="NP_001363161.1"
                     /db_xref="CCDS:CCDS10596.1"
                     /db_xref="GeneID:7783"
                     /db_xref="HGNC:HGNC:13188"
                     /db_xref="MIM:182888"
                     /translation="MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSGNSIDVSQL
                     VNPAFPGTVTCDEREITVEFPSSPGTKKWHASVVDPLGLDMPNCTYILDPEKLTLRAT
                     YDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPAMQVEETQGLSASTICQKDFM
                     SFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLPEAMKEGFSLLIDNHRMTF
                     HVPFNATGVTHYVQGNSHLYMVSLKLTFISPGQKVIFSSQAICAPDPVTCNATHMTLT
                     IPEFPGKLKSVSFENQNIDVSQLHDNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQF
                     YLASLKLTFLLRPETVSMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALD
                     LGTLRVGNSSCQPVFEAQSQGLVRFHIPLNGCGTRYKFEDDKVVYENEIHALWTDFPP
                     SKISRDSEFRMTVKCSYSRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQP
                     YGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWNVVVDGC
                     AYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLS
                     PDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGS
                     SGEKSRSETGEEVGSRGAMDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKR
                     TVSNH"
     exon            90..178
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            179..262
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            263..357
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            358..510
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            511..555
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            556..720
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            721..817
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            818..999
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1126
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1314
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1406
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1531
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1721
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1857
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..1954
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2038
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2122
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2295
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2277..2282
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /note="hexamer: AATAAA"
     polyA_site      2295
                     /gene="ZP2"
                     /gene_synonym="OOMD6; OZEMA6; Zp-2; ZPA"
                     /note="major polyA site"
ORIGIN      
        1 ctggttgatc tggtagtgtc ttctgctatg gcgtgcaggc agagaggagg ctcttggagt
       61 ccctcaggct ggttcaatgc aggctggagc acctacaggt cgatttctct cttcttcgcc
      121 cttgtgactt cagggaactc catagatgtt tctcagttgg taaatcctgc ctttccaggc
      181 actgtcactt gcgatgaaag ggaaataaca gtggagttcc caagcagtcc tggcaccaag
      241 aaatggcatg catctgtggt ggatcctctt ggtctcgaca tgccgaactg cacttacatc
      301 ctggacccag aaaagctcac cctgagggct acctatgata actgtaccag gagagtgcat
      361 ggtggacacc agatgaccat cagagtcatg aacaacagtg ctgccttaag acacggagct
      421 gtcatgtatc agttcttctg tccagctatg caagtagaag agacccaggg gctttcagca
      481 tctacaatct gccagaagga tttcatgtct ttttccttgc cacgggtctt ctctggcttg
      541 gctgacgaca gtaaggggac caaagttcag atgggatgga gcattgaggt tggtgatggt
      601 gcaagagcca aaactctgac cctgccagag gccatgaagg aaggcttcag cctcttgatt
      661 gacaaccaca ggatgacctt ccatgtgcca ttcaatgcca ctggagtgac tcactatgtg
      721 caaggtaaca gtcatctcta catggtgtct ctgaagctta catttatatc tcctggacag
      781 aaggtgatct tctcttcaca agctatttgt gcaccagatc ctgtgacctg caatgccaca
      841 cacatgactc tcaccatacc agagtttcct gggaagctta agtctgtgag ctttgaaaac
      901 cagaacattg atgtgagcca gctgcatgac aatggaattg atctagaagc aacaaatggc
      961 atgaaattgc atttcagcaa aactctgctc aaaacgaaat tatctgaaaa atgcctactc
     1021 catcagttct acttagcttc actcaagctg acctttctcc ttcggccaga gacagtatcc
     1081 atggtgatct atcctgagtg tctctgtgag tcacccgttt ctatagttac aggggagctg
     1141 tgcacccagg atgggtttat ggacgtcgag gtctacagct accaaacaca accagctctt
     1201 gacctgggta ctctgagggt gggaaactca tcctgccagc ctgtctttga ggctcagtct
     1261 caggggctgg tacggttcca catacccctg aatggatgtg gaacgagata taagttcgaa
     1321 gatgataaag tcgtctatga aaacgaaata catgctctct ggacggattt tcctccaagc
     1381 aaaatatcta gagacagtga gttcagaatg acagtgaagt gttcttatag caggaatgac
     1441 atgctactaa acatcaacgt tgaaagcctt actcctccag tggcctcagt gaagttgggt
     1501 ccatttacct tgatcctgca aagctaccca gataattcct accaacaacc ttatggggaa
     1561 aacgagtacc ctctagtgag attcctccgc caaccaattt acatggaagt gagagtccta
     1621 aacagggatg accccaacat caagctggtc ttagatgact gctgggcgac gtccaccatg
     1681 gatccagact ctttccccca gtggaacgtt gtcgtggatg gctgtgcata tgacctggac
     1741 aactaccaga ccaccttcca tccagtcggc tcctctgtga cccatcctga tcactatcag
     1801 aggtttgaca tgaaggcttt tgcctttgta tcagaagccc acgtgctctc tagcctggtc
     1861 tacttccact gcagtgcctt aatctgtaat cgactctccc ctgactcccc actgtgttct
     1921 gtgacctgcc ctgtgtcctc taggcacagg cgagccacag gggccactga agcagagaaa
     1981 atgacagtca gcctcccagg acccattctc ctgttgtcag atgactcctc attcagaggt
     2041 gtcggctcat ctgatctaaa agcaagtggg agcagtgggg agaagagtag gagtgaaaca
     2101 ggggaggagg ttggctcacg aggtgctatg gacaccaaag ggcacaagac tgctggagat
     2161 gttggttcca aagctgtggc tgctgtggct gcctttgcag gtgtggtggc aactctaggc
     2221 ttcatctact acctgtacga gaaaaggact gtgtcaaatc actaaatggg cttctaaata
     2281 aagcagtcaa aataa
//
