LOCUS       NM_001206998            6872 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens zinc and ring finger 3 (ZNRF3), transcript variant 1,
            mRNA.
ACCESSION   NM_001206998
VERSION     NM_001206998.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Boonsawat,P., Asadollahi,R., Niedrist,D., Steindl,K., Begemann,A.,
            Joset,P., Bhoj,E.J., Li,D., Zackai,E., Vetro,A., Barba,C.,
            Guerrini,R., Whalen,S., Keren,B., Khan,A., Jing,D., Palomares
            Bralo,M., Rikeros Orozco,E., Hao,Q., Schlott Kristiansen,B.,
            Zheng,B., Donnelly,D., Clowes,V., Zweier,M., Papik,M., Siegel,G.,
            Sabatino,V., Mocera,M., Horn,A.H.C., Sticht,H. and Rauch,A.
  TITLE     Deleterious ZNRF3 germline variants cause neurodevelopmental
            disorders with mirror brain phenotypes via domain-specific effects
            on Wnt/beta-catenin signaling
  JOURNAL   Am J Hum Genet 111 (9), 1994-2011 (2024)
   PUBMED   39168120
  REMARK    GeneRIF: Deleterious ZNRF3 germline variants cause
            neurodevelopmental disorders with mirror brain phenotypes via
            domain-specific effects on Wnt/beta-catenin signaling.
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Bugter,J.M., van Kerkhof,P., Jordens,I., Janssen,E., Tran Ngoc
            Minh,T., Iglesias van Montfort,D., Jamieson,C. and Maurice,M.M.
  TITLE     E3 ligases RNF43 and ZNRF3 display differential specificity for
            endocytosis of Frizzled receptors
  JOURNAL   Life Sci Alliance 7 (9), e202402575 (2024)
   PUBMED   38969364
  REMARK    GeneRIF: E3 ligases RNF43 and ZNRF3 display differential
            specificity for endocytosis of Frizzled receptors.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6872)
  AUTHORS   Kschonsak,Y.T., Gao,X., Miller,S.E., Hwang,S., Marei,H., Wu,P.,
            Li,Y., Ruiz,K., Dorighi,K., Holokai,L., Perampalam,P., Tsai,W.K.,
            Kee,Y.S., Agard,N.J., Harris,S.F., Hannoush,R.N. and de Sousa E
            Melo,F.
  TITLE     Potent and selective binders of the E3 ubiquitin ligase ZNRF3
            stimulate Wnt signaling and intestinal organoid growth
  JOURNAL   Cell Chem Biol 31 (6), 1176-1187 (2024)
   PUBMED   38056465
  REMARK    GeneRIF: Potent and selective binders of the E3 ubiquitin ligase
            ZNRF3 stimulate Wnt signaling and intestinal organoid growth.
REFERENCE   4  (bases 1 to 6872)
  AUTHORS   Liu,M., Zhao,H., Peng,S., Wu,Y., Liu,Y., Sun,W., Zen,K. and Sun,X.
  TITLE     Comprehensive analysis of zinc and ring finger 3 in prognostic
            value and pan-cancer immunity
  JOURNAL   FASEB J 38 (5), e23523 (2024)
   PUBMED   38457275
  REMARK    GeneRIF: Comprehensive analysis of zinc and ring finger 3 in
            prognostic value and pan-cancer immunity.
REFERENCE   5  (bases 1 to 6872)
  AUTHORS   Amano,N., Narumi,S., Aizu,K., Miyazawa,M., Okamura,K., Ohashi,H.,
            Katsumata,N., Ishii,T. and Hasegawa,T.
  TITLE     Single-Exon Deletions of ZNRF3 Exon 2 Cause Congenital Adrenal
            Hypoplasia
  JOURNAL   J Clin Endocrinol Metab 109 (3), 641-648 (2024)
   PUBMED   37878959
  REMARK    GeneRIF: Single-Exon Deletions of ZNRF3 Exon 2 Cause Congenital
            Adrenal Hypoplasia.
REFERENCE   6  (bases 1 to 6872)
  AUTHORS   Schleinitz,D., Kloting,N., Lindgren,C.M., Breitfeld,J.,
            Dietrich,A., Schon,M.R., Lohmann,T., Dressler,M., Stumvoll,M.,
            McCarthy,M.I., Bluher,M. and Kovacs,P.
  TITLE     Fat depot-specific mRNA expression of novel loci associated with
            waist-hip ratio
  JOURNAL   Int J Obes (Lond) 38 (1), 120-125 (2014)
   PUBMED   23670221
  REMARK    GeneRIF: Genes within recently identified loci associated with
            waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression,
            which correlates with obesity-related traits. Adipose tissue (AT)
            mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14
REFERENCE   7  (bases 1 to 6872)
  AUTHORS   Xie,Y., Zamponi,R., Charlat,O., Ramones,M., Swalley,S., Jiang,X.,
            Rivera,D., Tschantz,W., Lu,B., Quinn,L., Dimitri,C., Parker,J.,
            Jeffery,D., Wilcox,S.K., Watrobka,M., LeMotte,P., Granda,B.,
            Porter,J.A., Myer,V.E., Loew,A. and Cong,F.
  TITLE     Interaction with both ZNRF3 and LGR4 is required for the signalling
            activity of R-spondin
  JOURNAL   EMBO Rep 14 (12), 1120-1126 (2013)
   PUBMED   24165923
REFERENCE   8  (bases 1 to 6872)
  AUTHORS   Zhou,Y., Lan,J., Wang,W., Shi,Q., Lan,Y., Cheng,Z. and Guan,H.
  TITLE     ZNRF3 acts as a tumour suppressor by the Wnt signalling pathway in
            human gastric adenocarcinoma
  JOURNAL   J Mol Histol 44 (5), 555-563 (2013)
   PUBMED   23504200
  REMARK    GeneRIF: ZNRF3 inhibits gastric cancer cell growth and promotes
            cell apoptosis by affecting the Wnt/beta-catenin/TCF4 signalling
            pathway.
REFERENCE   9  (bases 1 to 6872)
  AUTHORS   Zebisch,M., Xu,Y., Krastev,C., MacDonald,B.T., Chen,M.,
            Gilbert,R.J., He,X. and Jones,E.Y.
  TITLE     Structural and molecular basis of ZNRF3/RNF43 transmembrane
            ubiquitin ligase inhibition by the Wnt agonist R-spondin
  JOURNAL   Nat Commun 4, 2787 (2013)
   PUBMED   24225776
  REMARK    GeneRIF: Signalling potency of R-spondin proteins depends on their
            ability to recruit ZNRF3 or RNF43 via Fu1 into a complex with LGR
            receptors, which interact with Rspo via Fu2
REFERENCE   10 (bases 1 to 6872)
  AUTHORS   Hao,H.X., Xie,Y., Zhang,Y., Charlat,O., Oster,E., Avello,M.,
            Lei,H., Mickanin,C., Liu,D., Ruffner,H., Mao,X., Ma,Q., Zamponi,R.,
            Bouwmeester,T., Finan,P.M., Kirschner,M.W., Porter,J.A.,
            Serluca,F.C. and Cong,F.
  TITLE     ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive
            manner
  JOURNAL   Nature 485 (7397), 195-200 (2012)
   PUBMED   22575959
  REMARK    GeneRIF: study provides new mechanistic insights into the
            regulation of Wnt receptor turnover, and reveals ZNRF3 as a
            tractable target for therapeutic exploration
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL031596.7, AB051436.1, AL021393.1 and AI244417.1.
            
            On May 17, 2019 this sequence version replaced NM_001206998.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB051436.1, CR456397.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000544604.7/ ENSP00000443824.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-330               AL031596.7         670-999
            331-2636            AB051436.1         1-2306
            2637-2637           AL021393.1         43930-43930
            2638-3404           AB051436.1         2308-3074
            3405-6871           AB051436.1         3076-6542
            6872-6872           AI244417.1         20-20               c
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.1"
     gene            1..6872
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /note="zinc and ring finger 3"
                     /db_xref="GeneID:84133"
                     /db_xref="HGNC:HGNC:18126"
                     /db_xref="MIM:612062"
     exon            1..495
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /inference="alignment:Splign:2.1.0"
     CDS             196..3006
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; novel C3HC4 type Zinc finger (ring finger);
                     RING-type E3 ubiquitin transferase ZNRF3; zinc/RING finger
                     protein 3; RING finger protein 203; E3 ubiquitin-protein
                     ligase ZNRF3; CTA-292E10.6"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase ZNRF3 isoform 1
                     precursor"
                     /protein_id="NP_001193927.1"
                     /db_xref="CCDS:CCDS56225.1"
                     /db_xref="GeneID:84133"
                     /db_xref="HGNC:HGNC:18126"
                     /db_xref="MIM:612062"
                     /translation="MRPRSGGRPGATGRRRRRLRRRPRGLRCSRLPPPPPLPLLLGLL
                     LAAAGPGAARAKETAFVEVVLFESSPSGDYTTYTTGLTGRFSRAGATLSAEGEIVQMH
                     PLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKAKRAVQRGATAVIFDVSE
                     NPEAIDQLNQGSEDPLKRPVVYVKGADAIKLMNIVNKQKVARARIQHRPPRQPTEYFD
                     MGIFLAFFVVVSLVCLILLVKIKLKQRRSQNSMNRLAVQALEKMETRKFNSKSKGRRE
                     GSCGALDTLSSSSTSDCAICLEKYIDGEELRVIPCTHRFHRKCVDPWLLQHHTCPHCR
                     HNIIEQKGNPSAVCVETSNLSRGRQQRVTLPVHYPGRVHRTNAIPAYPTRTSMDSHGN
                     PVTLLTMDRHGEQSLYSPQTPAYIRSYPPLHLDHSLAAHRCGLEHRAYSPAHPFRRPK
                     LSGRSFSKAACFSQYETMYQHYYFQGLSYPEQEGQSPPSLAPRGPARAFPPSGSGSLL
                     FPTVVHVAPPSHLESGSTSSFSCYHGHRSVCSGYLADCPGSDSSSSSSSGQCHCSSSD
                     SVVDCTEVSNQGVYGSCSTFRSSLSSDYDPFIYRSRSPCRASEAGGSGSSGRGPALCF
                     EGSPPPEELPAVHSHGAGRGEPWPGPASPSGDQVSTCSLEMNYSSNSSLEHRGPNSST
                     SEVGLEASPGAAPDLRRTWKGGHELPSCACCCEPQPSPAGPSAGAAGSSTLFLGPHLY
                     EGSGPAGGEPQSGSSQGLYGLHPDHLPRTDGVKYEGLPCCFYEEKQVARGGGGGSGCY
                     TEDYSVSVQYTLTEEPPPGCYPGARDLSQRIPIIPEDVDCDLGLPSDCQGTHSLGSWG
                     GTRGPDTPRPHRGLGATREEERALCCQARALLRPGCPPEEAGAVRANFPSALQDTQES
                     STTATEAAGPRSHSADSSSPGA"
     exon            496..621
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /inference="alignment:Splign:2.1.0"
     exon            622..696
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /inference="alignment:Splign:2.1.0"
     exon            697..828
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /inference="alignment:Splign:2.1.0"
     exon            829..939
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1107
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1210
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..2962
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /inference="alignment:Splign:2.1.0"
     exon            2963..6872
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3191..3196
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /note="hexamer: AACAAA"
     polyA_site      3209
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /note="major polyA site"
     regulatory      3350..3355
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /note="hexamer: AATATA"
     polyA_site      3373
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /note="major polyA site"
     regulatory      6847..6852
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /note="hexamer: AATACA"
     polyA_site      6872
                     /gene="ZNRF3"
                     /gene_synonym="BK747E2.3; RNF203"
                     /note="major polyA site"
ORIGIN      
        1 gataacccct cacgtggagc agatgaaagg gccgcggcgc gacggccggg ggagccgagc
       61 tgagcctgcg acccacaaag ccgccgccgc cgccgccgtg atggggctgt gaggcgtccg
      121 cccgcgttcg gtcctcagcc ggcccgcgac tatgcccggc cgcgcccgcc ctccgcgccc
      181 tcccgccgca ggaccatgag gccgcgctcg ggcgggcgcc caggggccac gggccgccgc
      241 cgccgccgcc tgcgccgccg cccccgcggc ctccggtgca gccgcctgcc gccgccgccg
      301 ccgctgccgc tgctgctcgg gctgctgctg gcggccgcgg ggcccggcgc ggcgcgggcc
      361 aaggagacgg cgttcgtgga ggtggtgctg ttcgagtcga gcccaagcgg cgattacacc
      421 acctacacca ccggcctcac gggccgcttc tcgcgggccg gggccacgct cagcgccgag
      481 ggcgagatcg tgcagatgca cccactgggc ctatgtaata acaatgacga agaggacttg
      541 tatgaatatg gctgggtagg agtggtgaag ctggaacagc cagaattgga cccgaaacca
      601 tgcctcactg tcctaggcaa ggccaagcga gcagtacagc ggggagctac tgcagtcatc
      661 tttgatgtgt ctgaaaaccc agaagctatt gatcagctga accagggctc tgaagacccg
      721 ctcaagaggc cggtggtgta tgtgaagggt gcagatgcca ttaagctgat gaacatcgtc
      781 aacaagcaga aagtggctcg agcaaggatc cagcaccgcc ctcctcgaca acccactgaa
      841 tactttgaca tggggatttt cctggctttc ttcgtcgtgg tctccttggt ctgcctcatc
      901 ctccttgtca aaatcaagct gaagcagcga cgcagtcaga attccatgaa caggctggct
      961 gtgcaggctc tagagaagat ggaaaccaga aagttcaact ccaagagcaa ggggcgccgg
     1021 gaggggagct gtggggccct ggacacactc agcagcagct ccacgtccga ctgtgccatc
     1081 tgtctggaga agtacattga tggagaggag ctgcgggtca tcccctgtac tcaccggttt
     1141 cacaggaagt gcgtggaccc ctggctgctg cagcaccaca cctgccccca ctgtcggcac
     1201 aacatcatag aacaaaaggg aaacccaagc gcggtgtgtg tggagaccag caacctctca
     1261 cgtggtcggc agcagagggt gaccctgccg gtgcattacc ccggccgcgt gcacaggacc
     1321 aacgccatcc cagcctaccc tacgaggaca agcatggact cccacggcaa ccccgtcacc
     1381 ttgctgacca tggaccggca cggggagcag agcctctatt ccccgcagac ccccgcctac
     1441 atccgcagct acccacccct ccacctggac cacagcctgg ccgctcaccg ctgcggcctg
     1501 gagcaccggg cctactcccc agcccacccc ttccgcaggc ccaagttgag tggccgcagc
     1561 ttctccaagg cagcttgctt ctcccagtat gagaccatgt accagcacta ctacttccag
     1621 ggcctcagct acccggagca ggaggggcag tccccaccta gcctcgcacc ccggggcccg
     1681 gcccgtgcct ttcctccgag cggcagtggc agcctgctct tccccaccgt ggtgcacgtg
     1741 gccccgccct cccacctgga gagcggcagc acgtccagct tcagctgcta tcacggccac
     1801 cgctcggtgt gcagtggcta cctggccgac tgcccaggca gcgacagcag cagcagcagc
     1861 agctccggcc agtgccactg ttcctccagt gactctgtgg tagactgcac tgaggtcagc
     1921 aaccagggcg tgtacgggag ctgctccacc ttccgcagct ccctcagcag cgactatgac
     1981 cccttcatct accgcagccg gagcccctgt cgtgccagtg aggcgggggg ctcgggcagc
     2041 tcgggccggg gacctgccct gtgcttcgag ggctccccgc ctcccgagga gctcccggcg
     2101 gtgcacagtc atggtgctgg gcggggcgag ccttggccgg gccctgcctc tccctcgggg
     2161 gatcaggtgt ccacctgcag cctggagatg aactacagca gcaactcctc cctggagcac
     2221 agggggccca atagctctac ctcagaagtg gggctcgagg cttctcctgg ggccgcccct
     2281 gacctcagga ggacctggaa ggggggccac gagttgccgt cgtgtgcctg ctgctgcgag
     2341 ccccagccct ccccagccgg gcctagcgcc ggagcagctg gcagcagcac cttgttcctg
     2401 gggccccacc tctacgaggg ctctggcccg gcgggtgggg agccccagtc aggaagctcc
     2461 cagggcttgt acggccttca ccccgaccat ttgcccagga cagatggggt gaaatacgag
     2521 ggtctgccct gctgcttcta tgaagagaag caggtggccc gcgggggcgg agggggcagc
     2581 ggctgctaca ctgaggacta ctcggtgagt gtgcagtaca cgctcaccga ggaaccaccg
     2641 cccggctgct accccggggc ccgggacctg agccagcgca tccccatcat tccagaggat
     2701 gtggactgtg atctgggcct gccctcggac tgccaaggga cccacagcct cggctcctgg
     2761 ggtgggacgc gaggcccgga taccccacgg ccccacaggg gcctgggagc aacccgggaa
     2821 gaggagcggg ctctgtgctg ccaggctagg gccctactgc ggcctggctg ccctccggag
     2881 gaggcgggtg ctgtcagggc caacttccct agtgccctcc aggacactca ggagtccagc
     2941 accactgcca ctgaggctgc aggaccgaga tctcactcag cagacagcag cagcccggga
     3001 gcctgagctc aggaggaact cttacctgga aattgggaac tgtatggaga ctccaaactg
     3061 acttctttca aaaaacaaaa acaaaaaatt tttttagctt tgacaaacac acaaaagtgg
     3121 taataaagag agccctcctt gtcaacccaa aatgtgagcc ccctgtggca aaaccacccc
     3181 ctaccccatt aacaaatcaa cagacaaaat tctccgagtc ctttgcctct tttgataaca
     3241 tgttgttctg ttttgtaaag tgtgtgtgct tggggttccg aggtgtggga ttgagttctc
     3301 tgctttgttt ttttttaaga tattgtatgt aaatgtaaaa agttatttaa atatatattt
     3361 taaagaaccc taactgccaa cttttgctga aaaagaaaaa aaaatcactg ctgcattaaa
     3421 tgaaccacat catgtgtaga tactgttgtc tccctgaagg gagctcaggc ctttgaaaag
     3481 ctcagggctt cacctgcctt agaaaatgaa ccagaaactt gaagtaaagc tagttgatag
     3541 gggtacaggc tctgaggagc agtgcaaaac tgcctctttc tttctcgtgg caaatcccaa
     3601 tgtacacgat ttcaggtctc agacgccatg cctctccagc ccacgccttt aggcaggtga
     3661 tggcagcagc taggaatagg gtgtacatga tccacagccc tgcggagcca ggtcaagccg
     3721 ctgctatgaa agctccaggg tgatggggac gattctgccc agtgtcctca gtctgtcccc
     3781 tcaggtcatg gtcccaagtg aaatgacaga gttcacagcc ctggtcttgg ctgaggtcca
     3841 ggtcatagta agggcatgtt cttggggccc tcgacctgaa ctctgaccct ccgggcaggg
     3901 aagaggaggt tgtccccttt ggttgtcctg gctttggagt cctttgcaaa aatattttgg
     3961 gccccctgcc actggctgca gaaatggctc gacggggtgt gtggggacag acacccagaa
     4021 ggaatgtact tttgtggcct tggtgtccga tggggctggg ggagagtgct ctccactgac
     4081 ccagcagcac acccatgtgc agtgcgcctg catctgtgtg ggggcagcca caccccttgg
     4141 ctgctgcttc cttgggctgc ctttctgggg gcatgtgact ggacctacga ggtctgcact
     4201 gagctccatt tgaatgatac ctttcctatc ccatttcccc cacggaagca ccgcttcagg
     4261 gttattcagt cctctgcctc atggctgaaa ttgctcatct cgtctgcaga tgtctactat
     4321 cctgtctacc taatgcacta ttatgtattg attctccatg agacagagag agagagagac
     4381 tatcagatag tttacaccca aagggtaggt ttttgtatat ttttccagcc ttttttatta
     4441 aggggaaggg gagagtttaa aaacccaaac cgttgtggtt ttaaggtgtt tcatttttaa
     4501 aagggagaga gaatctattt aaagctattt cagatcaggg attgtcatcc ttttttgtcc
     4561 aatgtattcc ttgttcttta aaaaaatttt ttttagagga aactaatatt agtctttgtg
     4621 ttcactaact cttctggtca cttgtattta tttattcatt cattcatcag atatttgttg
     4681 ccatctgaaa gaactggccc agtgggtctg aaagctcgct tgagaatagg aaacttgaga
     4741 cctggccccc tgtgggtagg agaacaagga ccacctgggt tctccagtct tgaacgagaa
     4801 tctcactctt atcagaatgt ttttcttaac ctcagcgtat gatgaggaaa tttacttatc
     4861 tctagctagg atttgacaaa ttccaacatc aaatgatcaa aacatttgcc actgaggctt
     4921 cactggtgag atccgttctc cgtcctcggg tgcagtccct tgggggctgc tcctcggact
     4981 gcgccccgca cacctgttat cgagggtgtg agaagcgcct aagctggtga catgtgatct
     5041 gggacgcctt catttctcgg gccaggagta gcagctgcta aggacagcag cttgcattgc
     5101 gtggttttag ggaagcaggg tctggctttt aatatgaact gcaaaaagca gcttctcact
     5161 gatatttttt tgttgttgtt tctggggggt ttttttgttt tgtttttaat gcctttgagt
     5221 gcatattttc ttcctcgtct gaaaccgaac tcccaaagtg gctttcttta gccctggctg
     5281 gaaaaccacc tctcaatagc cttaagcaat aaatagatga gtagagaatg tggcttcaac
     5341 tgggcttatt aaagtaagtg tgtctagttt tcacttgaac aagtgatagc tgcagatggc
     5401 gaaagaaacc catttaattt ttgtagctta caggtggtag aaacaaaaat gcaattttaa
     5461 aaccttaaat accaaatacc aaccattgcc tttttttttt ttgagatgga attttgctct
     5521 tgtcacccag gctggagtgc aatggcgcga tctcacctca ctgcaacctc tgcctcccgg
     5581 gtccaagtga ttctcctgcc tcagcctccc aagtagctgg gattacaggc atgcgccacc
     5641 acacccagct aattttgtat ttttggtaga gacagggtat ctccatgttg gtcaggctgg
     5701 tcttggattc ccgacctcag gtgatccgcc cacctcggcc tcccaaagtg ctgggattac
     5761 aggcgtgagc caccatgcct gcccagcaat accaaccatt gtcttttaaa ttcgtgttgg
     5821 cttctcagac agggagatca ctggaataaa ataaccgatg gtcttatttt gtcacacgta
     5881 aatcaaaaga aatgtcctct ttgaagttgt aagactccac caatgacaga cacccttttc
     5941 ggtggactct gagtggtgtg tagtggtttt atagccatgg aaactaggag tatctcactt
     6001 tccactgaga acccctgccc ccaatccctc taagttgggg tgtggcagtt gggcagggtc
     6061 aagtgaccca gccctggctg taggacagcc atatacagtg aagagttcta gaaccagcta
     6121 aaaatggaag tttgggtgtt taccaacaag gtacctcttt atggatgcag ccccagtaag
     6181 ctggctttaa ctctcagctc cttccctgtc tcctcctaat ccaagccctt ttataaaata
     6241 aagccccttc tgtcccactg ctcacatact tatgtgctgc tagtctctac tcgaagttcg
     6301 tgcaggacta atgcttttaa aatgaggtct aaaaaataat tactagtcga gactattatt
     6361 ctttaaacag aactgccttt ttctactctt tatgtaaact ctttctattg tgttggtcta
     6421 acaaggcact attttaaaat tttttaattt ttcccatagc acttaaaaga gattttgtaa
     6481 agaccttgct gtaaagattt tgtaataaaa tggtctaagg gctctttttc caacattacc
     6541 atttttaaaa aatgttttaa aagctagaag acaacttatg tatattctgt atatgtatag
     6601 cagcacattt catttatgga aatatgttct cagaatattt atttactaat atatttatct
     6661 taagccatgt cttatgttga gagtgtgaca ttgttggaat aatcattgaa aatgactaac
     6721 acaagaccct gtaaatacat gataattgca cacagatttt acatatttgc agaccaaaaa
     6781 tgatttaaaa caagttgtag tcttctatgg ttttgtaaca aattgtacac atgactgtaa
     6841 aaaaaaaata caattttatc aagtatgtgt ta
//
