LOCUS       NM_147128               3167 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens zinc and ring finger 2 (ZNRF2), mRNA.
ACCESSION   NM_147128
VERSION     NM_147128.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Nicolson,S., Manning,J.A., Lim,Y., Jiang,X., Kolze,E., Dayan,S.,
            Umargamwala,R., Xu,T., Sandow,J.J., Webb,A.I., Kumar,S. and
            Denton,D.
  TITLE     The Drosophila ZNRF1/2 homologue, detour, interacts with HOPS
            complex and regulates autophagy
  JOURNAL   Commun Biol 7 (1), 183 (2024)
   PUBMED   38360932
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Shi,F., Wu,Y., Wang,K., Wang,J., Liu,M. and Sun,X.
  TITLE     A pancancer analysis of the oncogenic role of ZNRF2 in human
            tumours
  JOURNAL   J Cell Mol Med 27 (21), 3296-3312 (2023)
   PUBMED   37551845
  REMARK    GeneRIF: A pancancer analysis of the oncogenic role of ZNRF2 in
            human tumours.
            Review article
REFERENCE   3  (bases 1 to 3167)
  AUTHORS   Cui,Z., Luo,Z., Lin,Z., Shi,L., Hong,Y. and Yan,C.
  TITLE     Long non-coding RNA TTN-AS1 facilitates tumorigenesis of papillary
            thyroid cancer through modulating the miR-153-3p/ZNRF2 axis
  JOURNAL   J Gene Med 21 (5), e3083 (2019)
   PUBMED   30811764
  REMARK    GeneRIF: The findings obtained in the present study indicate that
            TTN-AS1 facilitated PTC progression by regulating the
            miR-153-3p/ZNRF2 axis and activating the PI3K/Akt/mTOR pathway.
REFERENCE   4  (bases 1 to 3167)
  AUTHORS   Xiao,Q., Yang,Y., An,Q. and Qi,Y.
  TITLE     MicroRNA-100 suppresses human osteosarcoma cell proliferation and
            chemo-resistance via ZNRF2
  JOURNAL   Oncotarget 8 (21), 34678-34686 (2017)
   PUBMED   28416774
  REMARK    GeneRIF: Results detected significantly higher levels of ZNRF2 in
            osteosarcoma cells (OS) and provide evidence that ZNRF2 enhances OS
            cell growth and improves survival against doxorubicin.
REFERENCE   5  (bases 1 to 3167)
  AUTHORS   Hoxhaj,G., Caddye,E., Najafov,A., Houde,V.P., Johnson,C.,
            Dissanayake,K., Toth,R., Campbell,D.G., Prescott,A.R. and
            MacKintosh,C.
  TITLE     The E3 ubiquitin ligase ZNRF2 is a substrate of mTORC1 and
            regulates its activation by amino acids
  JOURNAL   Elife 5, e12278 (2016)
   PUBMED   27244671
  REMARK    GeneRIF: These findings uncover ZNRF2 as a component of the amino
            acid sensing machinery that acts upstream of Rag-GTPases and the
            V-ATPase to activate mTORC1.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3167)
  AUTHORS   Hoxhaj,G., Najafov,A., Toth,R., Campbell,D.G., Prescott,A.R. and
            MacKintosh,C.
  TITLE     ZNRF2 is released from membranes by growth factors and, together
            with ZNRF1, regulates the Na+/K+ATPase
  JOURNAL   J Cell Sci 125 (Pt 19), 4662-4675 (2012)
   PUBMED   22797923
  REMARK    GeneRIF: ZNRF1 and ZNRF2 are new players in regulation of the
            ubiquitous Na(+)/K(+)ATPase that is tuned to changing demands in
            many physiological contexts.
            Erratum:[J Cell Sci. 2022 Mar 15;135(6):jcs259936. doi:
            10.1242/jcs.259936.. PMID: 35267011]
REFERENCE   7  (bases 1 to 3167)
  AUTHORS   Ebstein,F., Lange,N., Urban,S., Seifert,U., Kruger,E. and
            Kloetzel,P.M.
  TITLE     Maturation of human dendritic cells is accompanied by functional
            remodelling of the ubiquitin-proteasome system
  JOURNAL   Int J Biochem Cell Biol 41 (5), 1205-1215 (2009)
   PUBMED   19028597
REFERENCE   8  (bases 1 to 3167)
  AUTHORS   Plans,V., Scheper,J., Soler,M., Loukili,N., Okano,Y. and
            Thomson,T.M.
  TITLE     The RING finger protein RNF8 recruits UBC13 for lysine 63-based
            self polyubiquitylation
  JOURNAL   J Cell Biochem 97 (3), 572-582 (2006)
   PUBMED   16215985
REFERENCE   9  (bases 1 to 3167)
  AUTHORS   Araki,T. and Milbrandt,J.
  TITLE     ZNRF proteins constitute a family of presynaptic E3 ubiquitin
            ligases
  JOURNAL   J Neurosci 23 (28), 9385-9394 (2003)
   PUBMED   14561866
  REMARK    GeneRIF: identification of ZNRF2; data suggest that ZNRF proteins
            play a role in the establishment and maintenance of neuronal
            transmission and plasticity via their ubiquitin ligase activity
            [ZNRF2]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF513707.1, AF527533.1 and AK025240.1.
            
            On Nov 23, 2018 this sequence version replaced NM_147128.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF513707.1, AF527533.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323037.5/ ENSP00000323879.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1997              AF513707.1         291-2287
            1998-2781           AF527533.1         1615-2398
            2782-3167           AK025240.1         1514-1899
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p14.3"
     gene            1..3167
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /note="zinc and ring finger 2"
                     /db_xref="GeneID:223082"
                     /db_xref="HGNC:HGNC:22316"
                     /db_xref="MIM:612061"
     exon            1..1230
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /inference="alignment:Splign:2.1.0"
     CDS             762..1490
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /EC_number="2.3.2.27"
                     /note="zinc finger/RING finger 2; RING-type E3 ubiquitin
                     transferase ZNRF2; protein Ells2; RING finger protein 202;
                     zinc/RING finger protein 2; zinc and ring finger 2, E3
                     ubiquitin protein ligase"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase ZNRF2"
                     /protein_id="NP_667339.1"
                     /db_xref="CCDS:CCDS5426.1"
                     /db_xref="GeneID:223082"
                     /db_xref="HGNC:HGNC:22316"
                     /db_xref="MIM:612061"
                     /translation="MGAKQSGPAAANGRTRAYSGSDLPSSSSGGANGTAGGGGGARAA
                     AAGRFPAQVPSAHQPSASGGAAAAAAAPAAPAAPRSRSLGGAVGSVASGARAAQSPFS
                     IPNSSSGPYGSQDSVHSSPEDGGGGRDRPVGGSPGGPRLVIGSLPAHLSPHMFGGFKC
                     PVCSKFVSSDEMDLHLVMCLTKPRITYNEDVLSKDAGECAICLEELQQGDTIARLPCL
                     CIYHKGCIDEWFEVNRSCPEHPSD"
     exon            1231..1326
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1432
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1512
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /inference="alignment:Splign:2.1.0"
     exon            1513..3167
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2031..2036
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /note="hexamer: AGTAAA"
     polyA_site      2050
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /note="major polyA site"
     regulatory      2759..2764
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /note="hexamer: AATAAA"
     regulatory      2776..2781
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /note="hexamer: AATACA"
     polyA_site      2779
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
     polyA_site      2799
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
     polyA_site      2823
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
     regulatory      3140..3145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
                     /note="hexamer: ATTAAA"
     polyA_site      3167
                     /gene="ZNRF2"
                     /gene_synonym="RNF202"
ORIGIN      
        1 aaacacacgg gccgggcgca ccctgcagcc gcgccgctcc gcggccttcc ggcgcggggc
       61 cggggaacct cctccccatc tgcgcacccc ccgccttcgc ggcccagatc ctcccgccag
      121 cccggcccct ccttcgcagg gggagcgagg cgacggttgc cgggaagcgc gcgccacctc
      181 tccctcccat ttttcgttct cccctcgcgc gccacccgtt tttcctcttt ctccgttaat
      241 aacagctggg tggctggggg aggagggaag gtggccccgg cggagtctgg gcgggcgcct
      301 cccactcagc cgccagccgc cgtgggagcc ggaggatggc ggcggtagca gcggccgccc
      361 gagaggaggc ggtgccgaga tcgggggcgc cgagcgcggc agcagagagc ggtagcggcc
      421 cgtcgtggcg caccagaacc gaaaccagcg gcagccgcac ggccacttga gccgcccctt
      481 cctgctggag ccagcgaggg gtgcctgcag ccgggacccc ttcctctccg cgtctcctcg
      541 tctcccgcgc ccgcgtcagg ccgtcggcct cgcccgccgc cccaagaaga gcgcgccggg
      601 cgccgactgc ccctctggac gccgggcggc ggccctggac gtgcgggggc ctctctgggc
      661 cggccgcggc gcctgggccc tgccctctag ctcccgcgct cgctcccgcc ctcccggctc
      721 tcggggcgca gcgcgcgggc ccggcccggg gcagggcgga catgggcgcc aaacagagcg
      781 gcccggccgc cgctaacggc cgcacgcgcg cgtactcggg ctcggatcta ccttccagta
      841 gcagcggagg cgccaatggg accgcgggcg gcggcggggg cgctcgggcc gccgccgcgg
      901 ggaggttccc ggctcaggtg cccagcgcgc accagcccag cgcctccggc ggcgccgcgg
      961 cggccgcggc ggccccggca gccccggcgg ccccgcgcag ccgctccctc ggcggggccg
     1021 tggggagcgt ggcgtcgggg gcccgcgcgg cgcagtcccc cttcagcatc ccgaacagca
     1081 gcagcggccc gtacggctcg caggactcgg tgcacagcag ccctgaggac ggcggcggcg
     1141 gccgggaccg gccggtgggc gggagccccg gcgggccgcg cctggtgatc ggctccttac
     1201 cagctcacct ctcgccgcac atgtttggag gatttaagtg ccctgtatgc tcaaaatttg
     1261 tatcctcaga tgaaatggat ttgcatcttg taatgtgttt aacaaagcca cgaataacct
     1321 ataatgagga tgtactgagt aaagatgctg gggaatgtgc aatatgcctt gaagaattgc
     1381 agcagggaga tactatagca cgactgcctt gtctatgcat atatcataaa ggctgcatag
     1441 atgaatggtt tgaagtaaat agatcttgcc ctgagcaccc ttcagattaa gcgtcagctt
     1501 cctgttttat aggttttctt gtcttgacaa gatgcttgaa aaaccaagag gatatgaaaa
     1561 tctgtctctg gagaaacaaa gacgcaggca tactcagcca gaaatctgag ttttgtgaga
     1621 cttggtaata cagagatgga caatcgtact ggggtaaaaa aaccctgctg aagagaggac
     1681 agtgaccaca gaactcagtg taccaaacat gcatacaaag gacacacagg gattttgaaa
     1741 atgctgcaca tcccttaata gtcatctaca taggtaatac tgataaacat tttgtattca
     1801 gacgccaaag ttaactgatt taaaagttga tttacttttt attaagttct ccagagctgc
     1861 acaactagtt atgttttgat ttgttttgtt ttttaatttg gggtctcttt gttttcccca
     1921 acataatgtt cataatgttt ctgcattcat ctgttcttaa attgaaaaac atataattta
     1981 cttcttataa attgaagtct taaatgtgaa accaagaaat gtaatcaagc agtaaaaaca
     2041 tctgaatgta gaccatgatc tcaagttctt ccattttctc ccccacgagt ggaaaataga
     2101 cttctacata ggaaagctaa aatatgttaa tatttttaaa ttaaaggttt aatatcagaa
     2161 tgcagtccaa agagcaaatc atattacata attacatttt aattaaatat agaatattct
     2221 actgaattgc aatttattaa atattcttat cctcttaaat aaaactgctc aacagttaat
     2281 cagcagtgaa tcatcttgca gctatgcaat ttaaaaaaaa tacagattac caatttcaag
     2341 tgctgccagc taaaataact gttttaacgg gtatcttttg tttgttcttt tcacttaatt
     2401 attttattgt gctttgcatc tccaggcagt tctctcacat ttgggtaaaa tgtttagcag
     2461 gctgtaaact taagaaaagg gtaaaataaa attttctgga gaggaacttg gaatttgagg
     2521 gagattttat atacctttaa aaactgtaat ttaattggga tgccaggttt atagcaattt
     2581 gcaactttaa ttttccagat aatctggagg ttagcatttg ataaatgatt ttttaaagta
     2641 gatatgaaga ttttgttaat ttataattta ttcatgtgtt attactgtaa ttgaaaatgt
     2701 tatagacact tttaaattca gtttgtgtag aaagaaatgt gttaaacaaa attatgttaa
     2761 taaatattcc cacataatac atctgaatgg ttaaaaatac tggaagagtt gtcagggcta
     2821 caatagtgaa tgtccttttt tttaatatca aaaatatcga taagtaggtt ttgtgttttc
     2881 aacgtaggga aaatgttatc agtgaatagg tggtgtactt tacttggtat aatttaaagt
     2941 tgcacagtaa gtactgtttc cttattttaa tctttcttta ctcataatgt atttaagaat
     3001 ttgctgtttt atttaacgct gctactctta atcttctcta aatttttccc cctactttct
     3061 tgcttctgtt tcacattttt taaaagggca agtacaggag caactgctgc tacccagaaa
     3121 aatgtgtgta ttataaataa ttaaagagtt tttaattgct ttctgta
//
