LOCUS       NM_023074               3192 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens zinc finger protein 649 (ZNF649), mRNA.
ACCESSION   NM_023074
VERSION     NM_023074.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3192)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 3192)
  AUTHORS   Helleboid,P.Y., Heusel,M., Duc,J., Piot,C., Thorball,C.W.,
            Coluccio,A., Pontis,J., Imbeault,M., Turelli,P., Aebersold,R. and
            Trono,D.
  TITLE     The interactome of KRAB zinc finger proteins reveals the
            evolutionary history of their functional diversification
  JOURNAL   EMBO J 38 (18), e101220 (2019)
   PUBMED   31403225
REFERENCE   5  (bases 1 to 3192)
  AUTHORS   Brant,S.R., Okou,D.T., Simpson,C.L., Cutler,D.J., Haritunians,T.,
            Bradfield,J.P., Chopra,P., Prince,J., Begum,F., Kumar,A., Huang,C.,
            Venkateswaran,S., Datta,L.W., Wei,Z., Thomas,K., Herrinton,L.J.,
            Klapproth,J.A., Quiros,A.J., Seminerio,J., Liu,Z., Alexander,J.S.,
            Baldassano,R.N., Dudley-Brown,S., Cross,R.K., Dassopoulos,T.,
            Denson,L.A., Dhere,T.A., Dryden,G.W., Hanson,J.S., Hou,J.K.,
            Hussain,S.Z., Hyams,J.S., Isaacs,K.L., Kader,H., Kappelman,M.D.,
            Katz,J., Kellermayer,R., Kirschner,B.S., Kuemmerle,J.F., Kwon,J.H.,
            Lazarev,M., Li,E., Mack,D., Mannon,P., Moulton,D.E., Newberry,R.D.,
            Osuntokun,B.O., Patel,A.S., Saeed,S.A., Targan,S.R.,
            Valentine,J.F., Wang,M.H., Zonca,M., Rioux,J.D., Duerr,R.H.,
            Silverberg,M.S., Cho,J.H., Hakonarson,H., Zwick,M.E., McGovern,D.P.
            and Kugathasan,S.
  TITLE     Genome-Wide Association Study Identifies African-Specific
            Susceptibility Loci in African Americans With Inflammatory Bowel
            Disease
  JOURNAL   Gastroenterology 152 (1), 206-217 (2017)
   PUBMED   27693347
  REMARK    GeneRIF: We detected African-specific SNPs at ZNF649 and LSAMP,
            with associations of genome-wide significance for ulcerative
            colitis.
REFERENCE   6  (bases 1 to 3192)
  AUTHORS   Pieragostino,D., Agnifili,L., Fasanella,V., D'Aguanno,S.,
            Mastropasqua,R., Di Ilio,C., Sacchetta,P., Urbani,A. and Del
            Boccio,P.
  TITLE     Shotgun proteomics reveals specific modulated protein patterns in
            tears of patients with primary open angle glaucoma naive to therapy
  JOURNAL   Mol Biosyst 9 (6), 1108-1116 (2013)
   PUBMED   23580065
REFERENCE   7  (bases 1 to 3192)
  AUTHORS   Weimann,M., Grossmann,A., Woodsmith,J., Ozkan,Z., Birth,P.,
            Meierhofer,D., Benlasfer,N., Valovka,T., Timmermann,B.,
            Wanker,E.E., Sauer,S. and Stelzl,U.
  TITLE     A Y2H-seq approach defines the human protein methyltransferase
            interactome
  JOURNAL   Nat Methods 10 (4), 339-342 (2013)
   PUBMED   23455924
REFERENCE   8  (bases 1 to 3192)
  AUTHORS   Yang,H., Yuan,W., Wang,Y., Zhu,C., Liu,B., Wang,Y., Yang,D., Li,Y.,
            Wang,C., Wu,X. and Liu,M.
  TITLE     ZNF649, a novel Kruppel type zinc-finger protein, functions as a
            transcriptional suppressor
  JOURNAL   Biochem Biophys Res Commun 333 (1), 206-215 (2005)
   PUBMED   15950191
  REMARK    GeneRIF: These results suggest that ZNF649 protein may act as a
            transcriptional repressor in mitogen-activated protein kinase
            signaling pathway to mediate cellular functions.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA782250.1, AK315487.1, AC011460.3 and AL713677.1.
            
            On Nov 23, 2018 this sequence version replaced NM_023074.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.56188.1,
                                           SRR1803611.101150.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142670, SAMEA2162328
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354957.8/ ENSP00000347043.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DA782250.1         36-38
            4-1809              AK315487.1         1-1806
            1810-2530           AC011460.3         107673-108393
            2531-3192           AL713677.1         2508-3169
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.41"
     gene            1..3192
                     /gene="ZNF649"
                     /note="zinc finger protein 649"
                     /db_xref="GeneID:65251"
                     /db_xref="HGNC:HGNC:25741"
                     /db_xref="MIM:611903"
     exon            1..104
                     /gene="ZNF649"
                     /inference="alignment:Splign:2.1.0"
     exon            105..306
                     /gene="ZNF649"
                     /inference="alignment:Splign:2.1.0"
     CDS             292..1809
                     /gene="ZNF649"
                     /codon_start=1
                     /product="zinc finger protein 649"
                     /protein_id="NP_075562.2"
                     /db_xref="CCDS:CCDS12843.1"
                     /db_xref="GeneID:65251"
                     /db_xref="HGNC:HGNC:25741"
                     /db_xref="MIM:611903"
                     /translation="MTKAQESLTLEDVAVDFTWEEWQFLSPAQKDLYRDVMLENYSNL
                     VSVGYQAGKPDALTKLEQGEPLWTLEDEIHSPAHPEIEKADDHLQQPLQNQKILKRTG
                     QRYEHGRTLKSYLGLTNQSRRYNRKEPAEFNGDGAFLHDNHEQMPTEIEFPESRKPIS
                     TKSQFLKHQQTHNIEKAHECTDCGKAFLKKSQLTEHKRIHTGKKPHVCSLCGKAFYKK
                     YRLTEHERAHRGEKPHGCSLCGKAFYKRYRLTEHERAHKGEKPYGCSECGKAFPRKSE
                     LTEHQRIHTGIKPHQCSECGRAFSRKSLLVVHQRTHTGEKPHTCSECGKGFIQKGNLN
                     IHQRTHTGEKPYGCIDCGKAFSQKSCLVAHQRYHTGKTPFVCPECGQPCSQKSGLIRH
                     QKIHSGEKPYKCSDCGKAFLTKTMLIVHHRTHTGERPYGCDECEKAYFYMSCLVKHKR
                     IHSREKRGDSVKVENPSTASHSLSPSEHVQGKSPVNMVTVAMVAGQCEFAHILHS"
     exon            307..433
                     /gene="ZNF649"
                     /inference="alignment:Splign:2.1.0"
     exon            434..529
                     /gene="ZNF649"
                     /inference="alignment:Splign:2.1.0"
     exon            530..3192
                     /gene="ZNF649"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2181
                     /gene="ZNF649"
                     /note="major polyA site"
     regulatory      3173..3178
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF649"
                     /note="hexamer: AATAAA"
     polyA_site      3192
                     /gene="ZNF649"
                     /note="major polyA site"
ORIGIN      
        1 agaattgact cgcgccctcg tccgccgcca taggccagtg ccggggttta agggccagga
       61 aaggaagcat tcagggaatt taggtgtagc cagaagaaaa tcaggtcctg gctccccaga
      121 agcaagagag ttcaaatgaa ggaaggagga ggttcctgga tgtggatgtc atcatttctg
      181 ggaacactct taaatggaga ctcagatttc ttagccaaaa tttagggagg atccagaaga
      241 aaccaaagac gaagcatccc agttcttggg tatttcctga aacagaagaa aatgacaaag
      301 gcccaggaat cactgaccct ggaggatgtg gctgtggact tcacctggga ggagtggcag
      361 ttcctgagcc ctgctcagaa ggacctgtac cgggatgtga tgttggagaa ctacagcaac
      421 cttgtgtcag tggggtatca agccggcaaa cctgatgccc tcaccaagtt ggaacaagga
      481 gaaccactat ggacactaga agatgaaatc cacagtccag cccacccaga aattgagaaa
      541 gctgatgatc atctgcagca gcccttgcaa aaccaaaaaa tactgaagag gacgggacaa
      601 cgctatgaac acggaagaac tttgaaatca tatttaggtt taaccaacca gagcagaaga
      661 tacaacagaa aggagcctgc tgagtttaat ggagatggag cttttctcca tgataatcat
      721 gaacaaatgc ctacggaaat tgaattccct gaaagtagaa aacccatcag caccaagtca
      781 caattcctta aacatcagca aacacacaac atagagaaag cccatgaatg cactgactgt
      841 gggaaagctt tcctcaagaa gtctcagctc actgagcata agagaattca tacaggaaag
      901 aaaccccacg tgtgtagctt gtgtgggaaa gccttctaca agaagtacag gctcactgaa
      961 cacgagagag ctcacagagg agagaaaccc cacgggtgta gcttgtgtgg gaaagccttc
     1021 tacaagaggt acaggctcac tgaacacgag agagctcaca aaggagagaa accatacggg
     1081 tgcagtgaat gtgggaaagc cttccccagg aaatctgagc ttactgaaca tcaaaggatt
     1141 cacacgggaa ttaagcccca tcaatgcagc gaatgtggga gagctttctc cagaaaatca
     1201 ctactcgttg tacatcagcg aactcataca ggagagaagc ctcatacatg cagtgaatgt
     1261 ggaaaaggct tcattcagaa gggcaatctc aacatacatc aacgaactca cactggagag
     1321 aaaccttatg gatgcattga ctgtggcaag gccttcagcc agaagtcttg ccttgtagca
     1381 catcagagat atcatacagg aaagactccc tttgtatgtc ctgaatgtgg gcaaccctgt
     1441 tcacagaagt caggactcat tagacatcag aaaattcact caggagagaa accctataaa
     1501 tgcagtgact gtgggaaagc cttccttaca aagacaatgc tcattgtaca tcacagaact
     1561 cacacgggag agagacccta tggctgtgat gagtgtgaga aagcttactt ctatatgtct
     1621 tgccttgtta aacataagag aatacactca agggagaaac ggggggattc agtgaaggtg
     1681 gaaaatcctt ccacagcaag tcacagctta agtcctagtg aacatgtgca ggggaaaagc
     1741 cctgttaata tggtaactgt ggcaatggtg gcagggcagt gtgagtttgc ccacatcctg
     1801 cattcatgat aaacagtttg ctgtttgatc atatagcctc cagcggaatg ctgagtttgt
     1861 catgtcccat gggcctttgg ctccctgcac taatatgtat agtagggttt acaagatatg
     1921 aaatatattt tactttttta tatcttataa acctcactac ccctcccaca atattgtttt
     1981 tcatttacta tcttgatcat agagtttggc tggggagggg ggcagtttta gaggcttcca
     2041 cttggtgttc ctcagaatga tatctcttac tccgggggcc aaggtagggg ttagcttttg
     2101 ttctctttgt agtttagatt gtatctcttg ccttgttcaa gttcacaaat ctttttgtgt
     2161 atacacatat gtacatgaaa atgatgttca tgctttttat tattttaccc ttcattattt
     2221 cattttttat agttctcata gctatgtctt tcagttcact aatctttttt ccacagtgtt
     2281 taatctgtca ttaatcccat ccaaagtatg ttttctctca gaaattgtaa tttttaacct
     2341 ctaataattt gacatgggtt tttccctgtt atatctttca tatctttatt tatcatggtt
     2401 ttacttttaa cctatttatg ctttcatatt taaagtgggt gtttagtggg caacatacag
     2461 ttgggccttc cttttttatc cagttaacaa tctctgtctt tttactgggg tatttagatc
     2521 atttacgttc aatgtattga tgtttttagg tttaaattac taacttacta tttgttgttt
     2581 attctatgtc ttctttgttc tccttttctt ctttttctgc tttcccttag agtagctgag
     2641 tgtgtttata ttacatattg tctcctttgt tggcttatta actgtaaatc cttgtcattt
     2701 gattccttga gattttatag catacatcat tgtgagggat tgcatattta tgtcctctcc
     2761 aaatttacat gttgaagtcc taatccctaa gggatggcat taggaagtgt gacctttgga
     2821 aggtaattac atcttgaaag tggaaccctc atgatgagat tatgtgtctt atgagacaac
     2881 acagaagaga gtttgttttc tctttctctg ctctttgcca tgtgaagaca gaatgagatg
     2941 accatgcata aaccaggaaa tggactctca ctggacacta gacctgccaa caccttgatt
     3001 gtgttttaga gtccagttct agagactcct cagcctctgg aacgtgagaa atgaatgttt
     3061 gttaaggcag tcagtcagtc tatggtgtat tttttctagt agcctgaact gactgaaaga
     3121 aaatcatgaa cttatcattc taacttcaag gacattatat cacttactgt ataataaaat
     3181 aatcttacag ta
//
