LOCUS       NM_015461               4887 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens zinc finger protein 521 (ZNF521), transcript variant
            1, mRNA.
ACCESSION   NM_015461
VERSION     NM_015461.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Scicchitano,S., Faniello,M.C. and Mesuraca,M.
  TITLE     Zinc Finger 521 Modulates the Nrf2-Notch Signaling Pathway in Human
            Ovarian Carcinoma
  JOURNAL   Int J Mol Sci 24 (19), 14755 (2023)
   PUBMED   37834202
  REMARK    GeneRIF: Zinc Finger 521 Modulates the Nrf2-Notch Signaling Pathway
            in Human Ovarian Carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Scicchitano,S., Montalcini,Y., Lucchino,V., Melocchi,V.,
            Gigantino,V., Chiarella,E., Bianchi,F., Weisz,A. and Mesuraca,M.
  TITLE     Enhanced ZNF521 expression induces an aggressive phenotype in human
            ovarian carcinoma cell lines
  JOURNAL   PLoS One 17 (10), e0274785 (2022)
   PUBMED   36191006
  REMARK    GeneRIF: Enhanced ZNF521 expression induces an aggressive phenotype
            in human ovarian carcinoma cell lines.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4887)
  AUTHORS   Chiarella,E., Aloisio,A., Scicchitano,S., Todoerti,K.,
            Cosentino,E.G., Lico,D., Neri,A., Amodio,N., Bond,H.M. and
            Mesuraca,M.
  TITLE     ZNF521 Enhances MLL-AF9-Dependent Hematopoietic Stem Cell
            Transformation in Acute Myeloid Leukemias by Altering the Gene
            Expression Landscape
  JOURNAL   Int J Mol Sci 22 (19), 10814 (2021)
   PUBMED   34639154
  REMARK    GeneRIF: ZNF521 Enhances MLL-AF9-Dependent Hematopoietic Stem Cell
            Transformation in Acute Myeloid Leukemias by Altering the Gene
            Expression Landscape.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4887)
  AUTHORS   Chiarella,E., Aloisio,A., Scicchitano,S., Bond,H.M. and Mesuraca,M.
  TITLE     Regulatory Role of microRNAs Targeting the Transcription Co-Factor
            ZNF521 in Normal Tissues and Cancers
  JOURNAL   Int J Mol Sci 22 (16), 8461 (2021)
   PUBMED   34445164
  REMARK    GeneRIF: Regulatory Role of microRNAs Targeting the Transcription
            Co-Factor ZNF521 in Normal Tissues and Cancers.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4887)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 4887)
  AUTHORS   Matsubara,E., Sakai,I., Yamanouchi,J., Fujiwara,H., Yakushijin,Y.,
            Hato,T., Shigemoto,K. and Yasukawa,M.
  TITLE     The role of zinc finger protein 521/early hematopoietic zinc finger
            protein in erythroid cell differentiation
  JOURNAL   J Biol Chem 284 (6), 3480-3487 (2009)
   PUBMED   19049973
  REMARK    GeneRIF: ZNF521 modulates erythroid cell differentiation through
            direct binding with GATA-1.
REFERENCE   7  (bases 1 to 4887)
  AUTHORS   Bond,H.M., Mesuraca,M., Amodio,N., Mega,T., Agosti,V., Fanello,D.,
            Pelaggi,D., Bullinger,L., Grieco,M., Moore,M.A., Venuta,S. and
            Morrone,G.
  TITLE     Early hematopoietic zinc finger protein-zinc finger protein 521: a
            candidate regulator of diverse immature cells
  JOURNAL   Int J Biochem Cell Biol 40 (5), 848-854 (2008)
   PUBMED   17543573
  REMARK    GeneRIF: A review of possible roles for ZNF521 in development, stem
            cell regulation and oncogenesis.
            Review article
REFERENCE   8  (bases 1 to 4887)
  AUTHORS   Bond,H.M., Mesuraca,M., Carbone,E., Bonelli,P., Agosti,V.,
            Amodio,N., De Rosa,G., Di Nicola,M., Gianni,A.M., Moore,M.A.,
            Hata,A., Grieco,M., Morrone,G. and Venuta,S.
  TITLE     Early hematopoietic zinc finger protein (EHZF), the human homolog
            to mouse Evi3, is highly expressed in primitive human hematopoietic
            cells
  JOURNAL   Blood 103 (6), 2062-2070 (2004)
   PUBMED   14630787
  REMARK    GeneRIF: EHZF is likely to play a relevant role in the control of
            human hematopoiesis and might be implicated in the development of
            hematopoietic malignancies.
REFERENCE   9  (bases 1 to 4887)
  AUTHORS   Warming,S., Liu,P., Suzuki,T., Akagi,K., Lindtner,S.,
            Pavlakis,G.N., Jenkins,N.A. and Copeland,N.G.
  TITLE     Evi3, a common retroviral integration site in murine B-cell
            lymphoma, encodes an EBFAZ-related Kruppel-like zinc finger protein
  JOURNAL   Blood 101 (5), 1934-1940 (2003)
   PUBMED   12393497
REFERENCE   10 (bases 1 to 4887)
  AUTHORS   Tchernev,V.T., Mansfield,T.A., Giot,L., Kumar,A.M., Nandabalan,K.,
            Li,Y., Mishra,V.S., Detter,J.C., Rothberg,J.M., Wallace,M.R.,
            Southwick,F.S. and Kingsmore,S.F.
  TITLE     The Chediak-Higashi protein interacts with SNARE complex and signal
            transduction proteins
  JOURNAL   Mol Med 8 (1), 56-64 (2002)
   PUBMED   11984006
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA102816.1, AK027354.1, BC113622.1 and AF141339.1.
            
            On Nov 23, 2018 this sequence version replaced NM_015461.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.215351.1,
                                           SRR1660805.199349.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968189,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361524.8/ ENSP00000354794.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                DA102816.1         85-98
            15-2049             AK027354.1         1-2035
            2050-3759           BC113622.1         1955-3664
            3760-4387           AK027354.1         3746-4373
            4388-4388           AF141339.1         886-886
            4389-4884           AK027354.1         4374-4869
            4885-4887           AF141339.1         1394-1396
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q11.2"
     gene            1..4887
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /note="zinc finger protein 521"
                     /db_xref="GeneID:25925"
                     /db_xref="HGNC:HGNC:24605"
                     /db_xref="MIM:610974"
     exon            1..162
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /inference="alignment:Splign:2.1.0"
     exon            163..203
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..4099
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     LYST-interacting protein 3; early hematopoietic zinc
                     finger protein"
                     /codon_start=1
                     /product="zinc finger protein 521 isoform 1"
                     /protein_id="NP_056276.1"
                     /db_xref="CCDS:CCDS32806.1"
                     /db_xref="GeneID:25925"
                     /db_xref="HGNC:HGNC:24605"
                     /db_xref="MIM:610974"
                     /translation="MSRRKQAKPRSLKDPNCKLEDKTEDGEALDCKKRPEDGEELEDE
                     AVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHG
                     EGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRS
                     RDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLH
                     GHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNE
                     DRAALQCVYCHELFVEETSLMNHMEQVHSGEKKNSCSICSESFHTVEELYSHMDSHQQ
                     PESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPD
                     MTGPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLY
                     NLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAK
                     DSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCT
                     NSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVG
                     GAPARPTGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKH
                     VTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLH
                     LAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVEL
                     QCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEV
                     ELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPG
                     ESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLL
                     TLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVT
                     STLELKIHGTFHMQKTGNGSAVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGL
                     PYGLCAGCVNLSKSASPGINVPPGTNRPGLGQNENLSAIEGKGKVGGLKTRCSSCNVK
                     FESESELQNHIQTIHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVF
                     YNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCF
                     TVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS"
     exon            204..383
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /inference="alignment:Splign:2.1.0"
     exon            384..3736
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /inference="alignment:Splign:2.1.0"
     exon            3737..3821
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /inference="alignment:Splign:2.1.0"
     exon            3822..3953
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..4069
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /inference="alignment:Splign:2.1.0"
     exon            4070..4887
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4865..4870
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /note="hexamer: AATAAA"
     polyA_site      4887
                     /gene="ZNF521"
                     /gene_synonym="EHZF; Evi3"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcaggc tgacaatgat ttcctcagtg attacgtaca gagcgagtcc ctgcgggtta
       61 ggggccccct ctggagccat cctgatggct ttgggggcct tgcttccatt ttccattatt
      121 atgtggacta ccggagcgac agcgcagtcc aagaccttgc aggatgtctc gccgcaagca
      181 agcgaaaccg agatccctca aagaccccaa ctgtaaactt gaagacaaga ctgaagatgg
      241 agaggcacta gattgtaaga agaggccgga agacggggag gagttggaag acgaagctgt
      301 gcacagctgt gacagctgcc tccaggtgtt tgaatcgctg agcgatatca cagaacacaa
      361 gattaatcaa tgtcaactga cagatggagt ggatgttgaa gatgatccga cttgctcttg
      421 gccagcttcc tcaccttcta gcaaggatca gacttcccct agccatggag aaggttgcga
      481 ttttggagag gaagaaggtg gccctgggct tccatacccg tgtcaattct gtgacaagtc
      541 gtttagccgc ctcagctacc taaagcacca tgagcagagt cacagtgaca aactgccttt
      601 caaatgcacc tactgcagta ggctgttcaa acacaagcgc agccgagatc gccacataaa
      661 actccacacc ggggacaaga agtaccactg cagtgaatgt gatgctgcgt tttccagaag
      721 tgatcacttg aagatccact taaagactca cacgtccaac aagccatata aatgtgccat
      781 ttgtcgccgt gggtttctgt cctctagttc cttacacgga cacatgcagg ttcatgagag
      841 gaacaaggac ggctctcagt ccggttccag gatggaggac tggaagatga aggacactca
      901 gaagtgcagt cagtgtgagg aaggctttga cttcccggaa gacctccaaa aacacattgc
      961 agagtgccac cccgaatgct ccccaaatga ggaccgagcg gccctccagt gtgtctactg
     1021 ccacgagctc ttcgtagagg agacctccct catgaaccac atggagcagg tgcatagcgg
     1081 ggagaagaag aactcatgca gcatttgttc tgagagtttc cacacagttg aggaactgta
     1141 cagccacatg gacagtcacc agcaaccgga gtcatgcaat cacagcaaca gcccttccct
     1201 ggtcacggtg ggctatacct ccgtgtccag tacgactcca gattccaacc tctcagtgga
     1261 cagctcaacc atggtggaag ctgccccgcc aatcccaaag agtcgaggga ggaagagggc
     1321 cgctcaacaa acccctgaca tgactggtcc ctcgagtaaa caagcaaaag ttacctacag
     1381 ctgtatttac tgcaacaaac aattattttc aagtcttgca gttctgcaga ttcacctgaa
     1441 aactatgcac ttagataagc cagaacaggc ccatatttgt cagtattgct tggaggtcct
     1501 gccctcactc tataacctaa atgaacatct taagcaagtg catgaagctc aggacccagg
     1561 tctgattgtt tctgccatgc ctgccattgt ctaccagtgt aacttctgtt ccgaagttgt
     1621 caacgacctc aacactcttc aggaacacat ccgatgttct catggatttg caaaccctgc
     1681 agctaaagat agtaatgcat tcttttgtcc ccattgctat atggggtttc tcactgactc
     1741 ttccctggaa gagcatatta gacaggttca ttgtgacctc agtggctccc gatttgggtc
     1801 tccagtgctt gggactccca aagaaccagt agtagaagtc tattcttgtt cctattgtac
     1861 aaattcgcca atattcaaca gcgttcttaa actgaacaag catatcaaag agaatcataa
     1921 aaacattccc ttggccctga attatatcca caatgggaag aaatccaggg ccttaagccc
     1981 cctatctcct gtggccatag agcagacatc tcttaagatg atgcaggcag taggaggtgc
     2041 acctgcacgt cccactggag aatatatctg taatcaatgt ggtgctaagt acacatccct
     2101 agacagcttt cagactcacc taaaaactca tctcgacact gtgcttccaa aattgacctg
     2161 tcctcagtgc aacaaggaat tccccaacca agaatccttg ctgaagcatg ttaccattca
     2221 ctttatgatc acttcaacgt attacatctg tgagagttgt gacaagcaat tcacatcagt
     2281 ggatgacctt cagaaacacc tgctggacat gcacaccttt gtcttctttc gctgcaccct
     2341 ctgccaggaa gtttttgact caaaagtctc cattcagctc cacttggctg tgaagcacag
     2401 taacgaaaag aaagtctata ggtgcacatc ttgcaactgg gacttccgca acgaaactga
     2461 cttgcagctc catgtgaaac acaaccacct ggaaaaccaa gggaaagtgc ataagtgcat
     2521 tttctgcggt gagtcctttg gcaccgaggt ggagctgcaa tgccacatca ccactcacag
     2581 taagaagtac aactgcaagt tctgtagcaa agccttccat gcgatcattt tgttagaaaa
     2641 acacttgcga gaaaaacact gtgtattcga aaccaagaca cccaactgtg gaacaaatgg
     2701 agcttccgag caagtgcaga aagaggaagt ggagctgcag actttgctga ccaacagcca
     2761 ggagtcccac aacagtcacg atgggagcga agaagacgtt gacacctctg agcctatgta
     2821 cggctgcgac atttgtgggg cagcctacac tatggaaact ttgctgcaga atcaccagct
     2881 ccgagaccac aacatcagac ctggagaaag tgccatcgtg aaaaagaaag ctgagctcat
     2941 taaagggaat tacaagtgca acgtgtgctc tcgaaccttc ttctccgaaa atggcctccg
     3001 ggaacatatg cagacccacc taggccctgt caaacactac atgtgcccta tttgcggaga
     3061 gcggtttccc tcccttttaa ctcttactga acacaaagtc acgcatagta agagtcttga
     3121 tactggaaac tgccggattt gcaagatgcc tctccagagt gaagaggagt ttttagagca
     3181 ttgccaaatg caccctgact tgaggaattc cctgacaggc tttcgctgcg tggtgtgcat
     3241 gcagacagtg acctccacct tggaactcaa aatccatggg acgttccaca tgcaaaagac
     3301 agggaatggg tctgcagttc agaccacagg gcggggccag cacgtccaaa aactgtataa
     3361 gtgcgcatct tgcctcaaag aattccgttc caagcaagat ctggtgaaac ttgatatcaa
     3421 tggcctgcca tatggtctgt gtgccggctg cgtgaatctc agtaagagcg ccagcccagg
     3481 cattaacgtc cctcccggca cgaatagacc aggcttgggc cagaatgaga atctgagtgc
     3541 cattgagggg aaaggcaagg tggggggact gaagacacgc tgctctagct gcaacgttaa
     3601 gtttgagtct gaaagtgaac tccagaacca catccaaacc atccaccgag agctcgtgcc
     3661 agacagcaac agcacacagt tgaaaacgcc ccaagtatca ccaatgccca gaatcagtcc
     3721 ctcccagtcg gatgagaaga agacctatca atgcatcaag tgtcagatgg ttttctacaa
     3781 tgaatgggat attcaggttc atgttgcaaa tcacatgatt gatgaaggac tgaaccatga
     3841 atgcaaactc tgcagccaga cctttgactc tcctgccaaa ctccagtgcc acctgataga
     3901 gcacagcttc gaagggatgg gaggcacctt caagtgtcca gtctgcttta cagtatttgt
     3961 tcaagcaaac aagttgcagc agcatatttt ctctgcccat ggacaagaag acaagatcta
     4021 tgactgtaca caatgtccac agaagttttt cttccaaaca gagctgcaga atcatacaat
     4081 gacccaacac agcagttagt gcaagtacag tctctcaagg agaattgatt ttgtggcaca
     4141 aaaagggaac atgttttact ctttgcacga aactttcatt gttaatgtat attattcaga
     4201 aacattgtat tgtaccataa aacttgtatt atcaaactgt tggatgttca tgtgtttgaa
     4261 cttttgcgca ccggatagac cccttgtata taaagtgttg cacatgtatt atgtcgtctg
     4321 atactaaaat ggtcttataa agacaagtgg acttgggccc tattcaggca agattaaaaa
     4381 aaaaaaaaga ctatgaccaa aatggcttaa gataaagtat ttttaaggaa gaaagattaa
     4441 aaacaactgt tatacatgag actatggttg gacttccttt tctttacact taagcctaga
     4501 atttctcttt aggtatatca gcgcttaaat ccaagactat tttttattgc tgaagattct
     4561 tgcaaaccat gaagagatgt tctcacagaa cagaacccca cagctggata aggcccgtat
     4621 atatatattt gtaagccttg caatgtgaca ggtagcatca ctatatatgc aatagttgtt
     4681 atgtagactg tcaaagaatt tttttttccc tggatacatt tgaagctttg agtgttcaag
     4741 gttttcctta atgatttcac gcagccaaat tcttgaatca gttgaactaa cctgtatgtt
     4801 actgttatta atgtttactc tgcagtctga acctggagat tactggaatt gttttccaag
     4861 aggaaataaa ttcagtttac cattagg
//
