LOCUS       NM_001396060            1401 bp    mRNA    linear   PRI 24-FEB-2023
DEFINITION  Homo sapiens ZNF511-PRAP1 readthrough (ZNF511-PRAP1), mRNA.
ACCESSION   NM_001396060
VERSION     NM_001396060.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Qiu GH, Leung CH, Yun T, Xie X, Laban M and Hooi SC.
  TITLE     Recognition and suppression of transfected plasmids by protein
            ZNF511-PRAP1, a potential molecular barrier to transgene expression
  JOURNAL   Mol Ther 19 (8), 1478-1486 (2011)
   PUBMED   21540836
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL360181.37.
            
            Summary: This locus represents naturally occurring readthrough
            transcription between the neighboring ZNF511 (zinc finger protein
            511) and PRAP1 (proline-rich acidic protein 1) genes on chromosome
            10. The putative readthrough transcript may encode a fusion protein
            that shares sequence identity with each individual gene product and
            may be involved in the regulation of gene promoters, particularly
            those found on transfected plasmids. [provided by RefSeq, Apr
            2017].
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2161674
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 118471, 118472
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-183               AL360181.37        16427-16609
            184-257             AL360181.37        16903-16976
            258-459             AL360181.37        17289-17490
            460-584             AL360181.37        17677-17801
            585-710             AL360181.37        19229-19354
            711-777             AL360181.37        57608-57674
            778-830             AL360181.37        58894-58946
            831-964             AL360181.37        59520-59653
            965-1401            AL360181.37        59760-60196
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.3"
     gene            1..1401
                     /gene="ZNF511-PRAP1"
                     /note="ZNF511-PRAP1 readthrough"
                     /db_xref="GeneID:104326056"
                     /db_xref="HGNC:HGNC:38088"
     exon            1..183
                     /gene="ZNF511-PRAP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1158
                     /gene="ZNF511-PRAP1"
                     /codon_start=1
                     /product="ZNF511-PRAP1 readthrough"
                     /protein_id="NP_001382989.1"
                     /db_xref="GeneID:104326056"
                     /db_xref="HGNC:HGNC:38088"
                     /translation="MQLPPALCARLAAGPGAAEPLPVERDPAAGAAPFRFVARPVRFP
                     REHQFFEDGDVQRHLYLQDVIMQVADVPEKPRVPAFACQVAGCCQVFDALDDYEHHYH
                     TLHGNVCSFCKRAFPSGHLLDAHILEWHDSLFQILSERQDMYQCLVEGCTEKFKTSRD
                     RKDHMVRMHLYPADFRFDKPKKSRSPASAEAPGDSGERSEGEAMEICSEPVAASPAPA
                     GERRIYRHRLLLVTSLVVVLLWEAGAVPAPKVPIKMQVKHWPSEQDPEKAWGARVVEP
                     PEKDDQLVVLFPVQKPKLLTTEEKPRGQGRGPILPGTKAWMETEDTLGHVLSPEPDHD
                     SLYHPPPEEDQGEERPRLWVMPNHQVLLGPEEDQDHIYHPQ"
     exon            184..257
                     /gene="ZNF511-PRAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            258..459
                     /gene="ZNF511-PRAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            460..584
                     /gene="ZNF511-PRAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            585..710
                     /gene="ZNF511-PRAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            711..777
                     /gene="ZNF511-PRAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            778..830
                     /gene="ZNF511-PRAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            831..964
                     /gene="ZNF511-PRAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1401
                     /gene="ZNF511-PRAP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1246..1251
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF511-PRAP1"
                     /note="hexamer: AATAAA"
     polyA_site      1266
                     /gene="ZNF511-PRAP1"
                     /note="major polyA site"
     regulatory      1377..1382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF511-PRAP1"
                     /note="hexamer: ACTAAA"
     regulatory      1383..1388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF511-PRAP1"
                     /note="hexamer: AATACA"
     polyA_site      1395
                     /gene="ZNF511-PRAP1"
     polyA_site      1401
                     /gene="ZNF511-PRAP1"
ORIGIN      
        1 gacaggctgc gcccgcccgc gcccggggtg atgcagttgc cccccgcgct gtgcgcccgc
       61 ctcgctgcgg ggcccggggc ggcggagccg ctgcctgtag agcgggatcc cgcggctggg
      121 gccgcgccct ttcgcttcgt tgcgcgcccc gtgcgcttcc cgcgggagca ccagttcttc
      181 gaggatgggg acgtgcagcg ccacctctac ctccaggacg tgatcatgca ggtggccgac
      241 gtgcctgaga agcccagggt gcccgcgttt gcctgccagg tggccggctg ctgccaggtg
      301 ttcgatgccc tggacgacta cgagcaccac taccacacgc tgcacggaaa tgtttgctcc
      361 ttttgcaagc gggccttccc ttccggacac ctgctggacg cccacatcct ggagtggcac
      421 gattcgctct tccagatcct gtctgagagg caggacatgt atcagtgctt ggtagaaggc
      481 tgcacagaga agttcaagac cagcagagac cggaaggatc acatggtgag gatgcacctg
      541 taccccgcgg acttccggtt tgataagcca aagaaaagca gaagcccagc ctcagcagaa
      601 gccccagggg acagtggaga gcggtcagaa ggggaggcca tggaaatctg ctctgagcct
      661 gtggcagcct cccctgcacc ggcaggtgag aggcggatct acagacatag gctcctcctg
      721 gtcaccagcc tggtggttgt gctgctgtgg gaggcaggtg cagtcccagc acccaaggtc
      781 cctatcaaga tgcaagtcaa acactggccc tcagagcagg acccagagaa ggcctggggc
      841 gcccgtgtgg tggagcctcc ggagaaggac gaccagctgg tggtgctgtt ccctgtccag
      901 aagccgaaac tcttgaccac cgaggagaag ccacgaggtc agggcagggg ccccatcctt
      961 ccaggcacca aggcctggat ggagaccgag gacaccctgg gccatgtcct gagtcccgag
     1021 cccgaccatg acagcctgta ccaccctccg cctgaggagg accagggcga ggagaggccc
     1081 cggttgtggg tgatgccaaa tcaccaggtg ctcctgggac cggaggaaga ccaagaccac
     1141 atctaccacc cccagtaggg ctccaggggc catcactgcc cccgccctgt cccaaggccc
     1201 aggctgttgg gactgggacc ctccctaccc tgccccagct agacaaataa accccagcag
     1261 gccgggcgcg gtggctcacc tctgtaatcc cagcactttt agaggccgag gcaggcggat
     1321 cacctgaaat caggagttcc agaccagcct gggcaacatg gtgaaacccc gtctctacta
     1381 aaaatacaaa aattagccgg g
//
