LOCUS       NM_017908               2050 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens zinc finger protein 446 (ZNF446), transcript variant
            1, mRNA.
ACCESSION   NM_017908
VERSION     NM_017908.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 2050)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2050)
  AUTHORS   Helleboid,P.Y., Heusel,M., Duc,J., Piot,C., Thorball,C.W.,
            Coluccio,A., Pontis,J., Imbeault,M., Turelli,P., Aebersold,R. and
            Trono,D.
  TITLE     The interactome of KRAB zinc finger proteins reveals the
            evolutionary history of their functional diversification
  JOURNAL   EMBO J 38 (18), e101220 (2019)
   PUBMED   31403225
REFERENCE   5  (bases 1 to 2050)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2050)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   7  (bases 1 to 2050)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   8  (bases 1 to 2050)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   9  (bases 1 to 2050)
  AUTHORS   Ravasi,T., Suzuki,H., Cannistraci,C.V., Katayama,S., Bajic,V.B.,
            Tan,K., Akalin,A., Schmeier,S., Kanamori-Katayama,M., Bertin,N.,
            Carninci,P., Daub,C.O., Forrest,A.R., Gough,J., Grimmond,S.,
            Han,J.H., Hashimoto,T., Hide,W., Hofmann,O., Kamburov,A., Kaur,M.,
            Kawaji,H., Kubosaki,A., Lassmann,T., van Nimwegen,E.,
            MacPherson,C.R., Ogawa,C., Radovanovic,A., Schwartz,A.,
            Teasdale,R.D., Tegner,J., Lenhard,B., Teichmann,S.A., Arakawa,T.,
            Ninomiya,N., Murakami,K., Tagami,M., Fukuda,S., Imamura,K., Kai,C.,
            Ishihara,R., Kitazume,Y., Kawai,J., Hume,D.A., Ideker,T. and
            Hayashizaki,Y.
  TITLE     An atlas of combinatorial transcriptional regulation in mouse and
            man
  JOURNAL   Cell 140 (5), 744-752 (2010)
   PUBMED   20211142
  REMARK    Erratum:[Cell. 2010 Apr 16;141(2):369. Kamburov, Atanas [added];
            Kaur, Mandeep [added]; MacPherson, Cameron Ross [added];
            Radovanovic, Aleksandar [added]; Schwartz, Ariel [added]]
REFERENCE   10 (bases 1 to 2050)
  AUTHORS   Liu,F., Zhu,C., Xiao,J., Wang,Y., Tang,W., Yuan,W., Zhao,Y., Li,Y.,
            Xiang,Z., Wu,X. and Liu,M.
  TITLE     A novel human KRAB-containing zinc-finger gene ZNF446 inhibits
            transcriptional activities of SRE and AP-1
  JOURNAL   Biochem Biophys Res Commun 333 (1), 5-13 (2005)
   PUBMED   15936718
  REMARK    GeneRIF: Overexpression of ZNF446 in COS-7 cells inhibits the
            transcriptional activities of SRE and AP-1.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA162028.1, AK000633.1 and AL122108.1.
            
            On May 17, 2019 this sequence version replaced NM_017908.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY280800.1, AK000633.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000594369.6/ ENSP00000472802.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-250               DA162028.1         189-438
            251-1362            AK000633.1         178-1289
            1363-2050           AL122108.1         533-1220
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.43"
     gene            1..2050
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /note="zinc finger protein 446"
                     /db_xref="GeneID:55663"
                     /db_xref="HGNC:HGNC:21036"
     exon            1..153
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /inference="alignment:Splign:2.1.0"
     exon            154..535
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /inference="alignment:Splign:2.1.0"
     CDS             194..1546
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /note="isoform 1 is encoded by transcript variant 1; zinc
                     finger protein with KRAB and SCAN domains 20"
                     /codon_start=1
                     /product="zinc finger protein 446 isoform 1"
                     /protein_id="NP_060378.1"
                     /db_xref="CCDS:CCDS12982.1"
                     /db_xref="GeneID:55663"
                     /db_xref="HGNC:HGNC:21036"
                     /translation="MPSPLGPPCLPVMDPETTLEEPETARLRFRGFCYQEVAGPREAL
                     ARLRELCCQWLQPEAHSKEQMLEMLVLEQFLGTLPPEIQAWVRGQRPGSPEEAAALVE
                     GLQHDPGQLLGWITAHVLKQEVLPAAQKTEEPLGSPHPSGTVESPGEGPQDTRIEGSV
                     QLSCSVKEEPNVDGQEVAPSSPPLAAQSPEGNHGHQEPASTSFHPPRIQEEWGLLDRS
                     QKELYWDAMLEKYGTVVSLGLPPHQPEAQAQSELGMLLTGTGVCRSLRSGNESEGPPG
                     CPEAQPPQGPGPAAWEGLSGAATPAPTVRPGTPPVPTQPTPAETRLEPAATPRKPYTC
                     EQCGRGFDWKSVFVIHHRTHTSGPGVQSPGLATGESTEKPPQGEVAFPHHPRRSLTGP
                     RSYPCEECGCSFSWKSQLVIHRKSHTGQRRHFCSDCGRAFDWKSQLVIHRKGHRPEVP
                     "
     exon            536..725
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /inference="alignment:Splign:2.1.0"
     exon            726..820
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /inference="alignment:Splign:2.1.0"
     exon            821..905
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /inference="alignment:Splign:2.1.0"
     exon            906..995
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /inference="alignment:Splign:2.1.0"
     exon            996..2050
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2029..2034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /note="hexamer: CATAAA"
     polyA_site      2050
                     /gene="ZNF446"
                     /gene_synonym="ZKSCAN20; ZSCAN30; ZSCAN52"
                     /note="major polyA site"
ORIGIN      
        1 ctttcctacc ctcaacgccg ttccgggggg ccgggcctgg caagtcctct tggaacgcct
       61 ccctcttgcc ttcccctttt ggggacagat cccgaagttc gagcatccct cggataggcc
      121 gggtgtcagg cctggtctct caggcccgtc caggcccatc ttgacgattc caagaccacc
      181 cccttgagca agaatgccat cccctctggg tcccccatgc ctgcccgtca tggacccaga
      241 gaccaccctt gaggagcctg agactgcccg cctccgcttc cgagggttct gctaccagga
      301 ggtggcaggt ccccgagaag ccctggcccg gctgcgtgag ctgtgttgcc agtggctgca
      361 gcctgaggca cactccaagg agcagatgct ggagatgctg gtgctggagc agttcctggg
      421 cacactgcct cccgagatcc aggcctgggt gcgcggtcag cggccaggca gtcctgagga
      481 ggccgctgcc ctagtcgaag gactgcagca tgaccctggg caactgttgg gctggatcac
      541 agcccatgtc ctgaagcagg aggtgctccc tgcagcccag aagacagagg aaccacttgg
      601 gagcccccac ccctcaggga cagtggagtc ccctggggaa ggtccccagg acaccagaat
      661 agaggggtct gtccagctca gctgcagtgt gaaggaggag cccaatgtcg atggacagga
      721 agtggcaccc tccagccctc cacttgcagc acagtcccct gaggggaacc atggacacca
      781 agaaccagcc tccacatcct tccacccacc caggattcag gaggagtggg ggctgctgga
      841 ccggtcacag aaggaactgt actgggatgc gatgctggag aagtacggca cagtggtctc
      901 cctggggtta ccgccccacc agccagaggc acaggcccag tcagagctgg ggatgctgct
      961 cacggggaca ggcgtctgca gaagcctgcg ctcgggaaat gagagtgagg gtccacctgg
     1021 ctgcccagag gcccagccgc cccagggccc agggccggca gcctgggagg gcttgtctgg
     1081 ggctgccact cctgccccca ctgtgcgccc agggacaccg ccagtgccca ctcagcccac
     1141 acctgcagag acgagactgg agccggctgc cacccccagg aagccctaca cgtgcgagca
     1201 gtgtggccgc ggcttcgact ggaagtcagt gttcgtcatc caccaccgga cacacacgag
     1261 tgggccaggt gtgcagtccc cggggctagc caccggggaa agcacagaga agccaccaca
     1321 aggggaggtg gcctttccgc accacccccg acgctcactc acaggccccc ggagttaccc
     1381 gtgtgaggag tgcgggtgca gcttcagctg gaagtcgcag ctggtcatcc accgcaagag
     1441 ccacacaggc cagcggcgtc acttctgcag tgactgtggc cgcgccttcg actggaagtc
     1501 gcagctggtc atccaccgca agggccaccg gccggaggtt ccatgagcag ccagacagca
     1561 cagtccctcg gggcctcggt gttctcgggg cctggataca gcctctgggg caccagcaga
     1621 agactctgga ggcagcaggg gatgccagag tgaacaaggg gtcccaagcc agttccctgc
     1681 ccctggtctg gtctccccca aaagacctgg gtgcaaggaa aaggagctgc tctctctctt
     1741 cttgcccctg cctcctagag ggaggtctgg gttcccttct atggctgacc agtgcctgtg
     1801 gggtgactgc caagcaccag gctccctccc tccctgtgac atggcctggg ctgacaacac
     1861 tccctctcct gggacctcct tgcctcaggt gggtgttcaa aaactgtgcc ttcccactcg
     1921 tctgtgcaga ggctgggcct gaggtctcag tgtggagagc agcagaagac ccaggaaagc
     1981 acagttggct tccgtttctc ctgctccctg tgtgtgttag aattttaaca taaattccac
     2041 tttcataata
//
