LOCUS       NM_144689               3639 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens zinc finger protein 420 (ZNF420), transcript variant
            2, mRNA.
ACCESSION   NM_144689
VERSION     NM_144689.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Olcina,M.M., Leszczynska,K.B., Senra,J.M., Isa,N.F., Harada,H. and
            Hammond,E.M.
  TITLE     H3K9me3 facilitates hypoxia-induced p53-dependent apoptosis through
            repression of APAK
  JOURNAL   Oncogene 35 (6), 793-799 (2016)
   PUBMED   25961932
  REMARK    GeneRIF: Functional importance of H3K9me3 in hypoxia, apoptosis and
            repression of APAK.
REFERENCE   3  (bases 1 to 3639)
  AUTHORS   Gaykalova,D.A., Vatapalli,R., Wei,Y., Tsai,H.L., Wang,H., Zhang,C.,
            Hennessey,P.T., Guo,T., Tan,M., Li,R., Ahn,J., Khan,Z.,
            Westra,W.H., Bishop,J.A., Zaboli,D., Koch,W.M., Khan,T., Ochs,M.F.
            and Califano,J.A.
  TITLE     Outlier Analysis Defines Zinc Finger Gene Family DNA Methylation in
            Tumors and Saliva of Head and Neck Cancer Patients
  JOURNAL   PLoS One 10 (11), e0142148 (2015)
   PUBMED   26544568
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3639)
  AUTHORS   Wang,S., Wang,S., Yang,L., Guo,H., Kong,X., Yuan,L., Xing,G., He,F.
            and Zhang,L.
  TITLE     ARF-mediated SUMOylation of Apak antagonizes ubiquitylation and
            promotes its nucleolar accumulation to inhibit 47S pre-rRNA
            synthesis
  JOURNAL   J Mol Cell Biol 7 (2), 154-167 (2015)
   PUBMED   25691462
  REMARK    GeneRIF: ARF coordinates Apak to regulate ribosome biogenesis upon
            cellular stress.
REFERENCE   5  (bases 1 to 3639)
  AUTHORS   Yuan,L., Tian,C., Wang,H., Song,S., Li,D., Xing,G., Yin,Y., He,F.
            and Zhang,L.
  TITLE     Apak competes with p53 for direct binding to intron 1 of p53AIP1 to
            regulate apoptosis
  JOURNAL   EMBO Rep 13 (4), 363-370 (2012)
   PUBMED   22334068
  REMARK    GeneRIF: Apak competes with p53 for binding to inhibit p53AIP1
            expression.
REFERENCE   6  (bases 1 to 3639)
  AUTHORS   Wang,S., Tian,C., Xing,G., Gao,M., Jiao,W., Xiao,T., Yin,Y., He,F.
            and Zhang,L.
  TITLE     ARF-dependent regulation of ATM and p53 associated KZNF (Apak)
            protein activity in response to oncogenic stress
  JOURNAL   FEBS Lett 584 (18), 3909-3915 (2010)
   PUBMED   20713054
  REMARK    GeneRIF: Apak is differentially regulated in the ARF and
            ATM-dependent manner in response to oncogenic stress and DNA
            damage, respectively.
REFERENCE   7  (bases 1 to 3639)
  AUTHORS   Wang,S., Tian,C., Xiao,T., Xing,G., He,F., Zhang,L. and Chen,H.
  TITLE     Differential regulation of Apak by various DNA damage signals
  JOURNAL   Mol Cell Biochem 333 (1-2), 181-187 (2010)
   PUBMED   19629643
  REMARK    GeneRIF: four of seven types of DNA damage signals examined
            resulted in significant Apak phosphorylation and dissociation of
            Apak from p53, releasing the inhibition of p53 transcriptional
            activity
            Erratum:[Mol Cell Biochem. 2010 Jan;333(1-2):189. Xiao, Tingtiang
            [corrected to Xiao, Tingting]]
REFERENCE   8  (bases 1 to 3639)
  AUTHORS   Tian,C., Xing,G., Xie,P., Lu,K., Nie,J., Wang,J., Li,L., Gao,M.,
            Zhang,L. and He,F.
  TITLE     KRAB-type zinc-finger protein Apak specifically regulates
            p53-dependent apoptosis
  JOURNAL   Nat Cell Biol 11 (5), 580-591 (2009)
   PUBMED   19377469
  REMARK    GeneRIF: Apak (ZNF420 or FLJ32191) is a negative regulator of
            p53-mediated apoptosis.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK315728.1 and AC012309.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_144689.4.
            
            Summary: The protein encoded by this gene is a KRAB-type zinc
            finger protein that negatively-regulates p53-mediated apoptosis.
            Under stress conditions, the encoded protein is phosphorylated by
            ATM and dissociates from p53, which activates p53 and initiates
            apoptosis. [provided by RefSeq, Jul 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.225604.1,
                                           SRR1803612.259816.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000337995.4/ ENSP00000338770.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2329              AK315728.1         15-2343
            2330-3639           AC012309.8         42284-43593
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.12"
     gene            1..3639
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /note="zinc finger protein 420"
                     /db_xref="GeneID:147923"
                     /db_xref="HGNC:HGNC:20649"
                     /db_xref="MIM:617216"
     exon            1..138
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /inference="alignment:Splign:2.1.0"
     exon            139..182
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /inference="alignment:Splign:2.1.0"
     exon            183..271
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /inference="alignment:Splign:2.1.0"
     CDS             263..2329
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /note="isoform b is encoded by transcript variant 2; ATM
                     and p53-associated KZNF protein"
                     /codon_start=1
                     /product="zinc finger protein 420 isoform b"
                     /protein_id="NP_653290.2"
                     /db_xref="CCDS:CCDS12498.1"
                     /db_xref="GeneID:147923"
                     /db_xref="HGNC:HGNC:20649"
                     /db_xref="MIM:617216"
                     /translation="MARKLVMFRDVAIDFSQEEWECLDSAQRDLYRDVMLENYSNLVS
                     LDLPSRCASKDLSPEKNTYETELSQWEMSDRLENCDLEESNSRDYLEAKGKMEKQQEN
                     QKEYFRQGMIIYDKMSIFNQHTYLSQHSRCHSTEKPYKCKECGKAFRRASHLTQHQSI
                     HTGEKPYECKQCGKAFSRDSQLSLHQRLHTGEKPYACKECGKAFTQSSQLILHHRIHT
                     GEKPYKCEECGKAFIRSSQLTRHQKVHTGEKPYECKECGKAFTQNSQLTLHQRLHTGE
                     KLYECKECRKVFTQLSQLILHKRIHTGEKPYECKECGKAFICGSQLSQHQKIHNGEKP
                     YECKECGRAFIRGSLLMQHQRIHTGEKPYKCEECGKAFIRGSQLTQHQRIHTNEKPYE
                     CKECGKMFSHGSQLTQHQRIHTGEKPYQCKECGKAFNRGSLLTRHQRIHTGEKPYECK
                     ECGKTFSRGSELTQHERIHTGEKPYECKECGKSFIRGSQLTQHQRIHTGEKPYECKEC
                     RMAFTQSSHLSQHQRLHTGEKPYVCNECGKAFARGLLLIQHQRIHTGEKPYQCKECGK
                     AFIRGSQLTQHQRIHTGEKPYECKECGKAFSHGSQLTLHQRIHTGEKPYECRECRKAF
                     TQSSHLSRHQRIHTGEKPYQCKECGKAFTRGSQLTQHQRIHISEKSFEYKECGIDFSH
                     GSQVYM"
     exon            272..398
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /inference="alignment:Splign:2.1.0"
     exon            399..3639
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3564..3569
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /note="hexamer: AATAAA"
     polyA_site      3582
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /note="major polyA site"
     regulatory      3615..3620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF420"
                     /gene_synonym="APAK"
                     /note="hexamer: AATAAA"
     polyA_site      3639
                     /gene="ZNF420"
                     /gene_synonym="APAK"
ORIGIN      
        1 gagatttgga ggcctcgcgt ggggagaggg ccgcgtctgt ggaggagctt ggctccgcac
       61 ctgctgggct ggcgaacccg aaattgggct tggaacccgg gagccagatc ttggaccctg
      121 aactgcacct ctgtcaagag cccagaggag aaagaatggc tgtttcagta gcaatgtcca
      181 agctctgcat tctccagact ctgtgctttc ctaagatagg aacccagaag aggactgatc
      241 atttcttgca gctctaaaaa ccatggctcg gaaattagtg atgttcaggg atgttgccat
      301 tgacttctct caggaagagt gggaatgcct ggactctgct cagagagatt tgtatagaga
      361 tgtgatgttg gagaactata gcaacttggt atcactagac ttgccttcaa ggtgtgcaag
      421 taaggactta tctccagaaa agaacactta tgaaacagaa ttatcccaat gggaaatgag
      481 tgacagactt gaaaactgtg atcttgaaga gtccaattcc agggattatt tggaagccaa
      541 aggcaagatg gagaagcaac aagaaaatca gaaggaatat ttcaggcaag ggatgatcat
      601 atatgacaaa atgtccattt tcaaccagca tacttactta tctcaacatt caagatgtca
      661 ttctactgag aaaccctata aatgtaagga atgtgggaaa gccttcagac gagcctcaca
      721 cctaacacaa catcaaagta ttcatactgg tgaaaaaccc tatgaatgta agcaatgcgg
      781 gaaggccttt agtcgtgatt cacaactcag tcttcatcag agacttcata ctggtgagaa
      841 accctatgca tgtaaggaat gtgggaaggc ctttactcaa agctcacaac ttattttaca
      901 tcatagaatt catactggtg aaaaaccata taaatgtgaa gaatgtggga aagcctttat
      961 tcgtagctca caacttaccc gacatcaaaa agttcatact ggtgagaaac cttatgaatg
     1021 taaagaatgt gggaaggcct ttactcagaa ttcacaactt acactacacc agagacttca
     1081 tactggtgaa aagctctatg aatgtaaaga atgtaggaag gtctttactc agctctcaca
     1141 acttattctg cataagagaa ttcataccgg tgagaaaccc tatgaatgta aggaatgtgg
     1201 aaaagctttt atttgtggct cacagctttc tcaacatcag aaaattcata atggggaaaa
     1261 accatatgaa tgtaaggaat gtggaagggc ctttattcgg ggctcactac tgatgcaaca
     1321 tcagaggatt catactggtg aaaaacccta taaatgtgaa gaatgtggga aggcctttat
     1381 ccgtggctca caacttactc aacaccagag aattcacacc aatgaaaagc cctatgaatg
     1441 taaggaatgt ggaaagatgt ttagtcatgg ctcacaactt actcaacatc agagaataca
     1501 cactggtgag aaaccctatc aatgtaagga atgtggaaaa gcgtttaatc gtggctcact
     1561 ccttacacga caccagagga ttcatactgg tgagaaaccc tatgaatgta aagaatgtgg
     1621 aaaaaccttt agtcgtggct cagaacttac tcaacatgag cgaattcaca caggtgagaa
     1681 accctatgaa tgtaaggaat gtgggaaatc ttttattcgt ggttcccagc ttactcaaca
     1741 tcagagaatc catactggtg agaaacctta tgaatgtaaa gaatgtagaa tggcctttac
     1801 tcagagttca catctttccc aacatcaaag acttcacact ggtgagaaac cctatgtgtg
     1861 taatgaatgt ggaaaggcct ttgcgcgtgg cttactactt atacaacatc agagaattca
     1921 tactggtgag aaaccatatc aatgtaagga atgtgggaaa gcctttattc gtggttcaca
     1981 gttgactcaa catcagcgaa ttcacactgg agaaaaaccc tatgaatgca aggagtgtgg
     2041 caaggccttt agtcatggct ctcagcttac tctacatcag agaatccata ctggtgagaa
     2101 gccctatgaa tgcagagaat gtagaaaggc ctttactcag agttcacatc tttctcggca
     2161 tcagagaatt catactggtg agaaaccata tcaatgtaag gaatgtggga aggcctttac
     2221 tcgtggttca cagctaactc aacatcagag aattcatatc agtgagaaat cttttgaata
     2281 taaggaatgt gggattgact ttagtcatgg ctcacaagtt tacatgtgaa ttgtctgatt
     2341 atttgagatc actatgaaga ggttctctgg ttgttagcag caaagaattc tcacaaatgt
     2401 gaatatgggc gcacatttgc ctcataaagc acagcatcag ataatttatg tgagagaaaa
     2461 tggtagtgtc attcatatag aaaaacatca ttactggaaa cctattaaac attagcaaat
     2521 tggagaatag ttttaatata gtaaatgtag gaagcccttt agccatattg aaaacaaata
     2581 tctttttcaa cgttatctta gctctactag ttgatctttt tgttatatgt atcatgatac
     2641 ttaacctcta ccttggttta atcattttaa gatagaccta agtatattac ctttattgta
     2701 agattcttgg aagtattatg taagttatta catgtaaaag ctcttagaat ggtgccttga
     2761 acgtagcata ccacaaatat tagctaccat tttcactagt gttattttag agaatttgca
     2821 tgagaggaga gtacttatga gtataatgaa tattgagaaa tcttttatca acacatccaa
     2881 gatgacttgg ttggggggca ctgtatgcca taatgaatgt gagaaagctg tcatttaaat
     2941 ctcatccatt attgctataa gaggaaattc atactgttaa aaaaaaaaaa acccagtgga
     3001 tataatcagt gtattattaa aaaaaaaaaa acccagtgga tgtaatcagt gtattattga
     3061 gcacagctgt tagagaagtg ggacaaggtg tgaagtgatt tttaacgaag tctaagatcc
     3121 aaagtctaat aaatctagga attttttaaa aacttgaatg tattgctttt gaagtaaaca
     3181 aaataactga tattacagac tattttgcaa tgaaaaatga tgagagtttg tgatacagac
     3241 tgcttttttc ctaccctata tctattttct cttttttagt aacaaatttc tgggctgaaa
     3301 atctcagcct tccttgcagg tcaacaagat agaagtgata tttatatgag taggagattt
     3361 acgaaatcca tttttcctgt ctttttttcc gtgcctacaa tgtggacatc taagctggag
     3421 ctccgttact ctcatgggga ctttgagaat ggtatctggg tgttatggat catggagcag
     3481 aaatacagaa ggagtctgca accctgatga ctttgtggaa cagccatact agctctggtc
     3541 tgcttgcctc ctgtttctct ttaaataaaa ttaaacatct tatttgttga tgctattgta
     3601 gttctgttat tgacaataaa aattcttaag gatataaaa
//
