LOCUS       NM_020657               4423 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens zinc finger protein 304 (ZNF304), transcript variant
            2, mRNA.
ACCESSION   NM_020657
VERSION     NM_020657.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Krasnopolsky,S., Kuzmina,A. and Taube,R.
  TITLE     Genome-wide CRISPR knockout screen identifies ZNF304 as a silencer
            of HIV transcription that promotes viral latency
  JOURNAL   PLoS Pathog 16 (9), e1008834 (2020)
   PUBMED   32956422
  REMARK    GeneRIF: Genome-wide CRISPR knockout screen identifies ZNF304 as a
            silencer of HIV transcription that promotes viral latency.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Aslan,B., Monroig,P., Hsu,M.C., Pena,G.A., Rodriguez-Aguayo,C.,
            Gonzalez-Villasana,V., Rupaimoole,R., Nagaraja,A.S., Mangala,S.,
            Han,H.D., Yuca,E., Wu,S.Y., Ivan,C., Moss,T.J., Ram,P.T., Wang,H.,
            Gol-Chambers,A., Ozkayar,O., Kanlikilicer,P., Fuentes-Mattei,E.,
            Kahraman,N., Pradeep,S., Ozpolat,B., Tucker,S., Hung,M.C.,
            Baggerly,K., Bartholomeusz,G., Calin,G., Sood,A.K. and
            Lopez-Berestein,G.
  TITLE     The ZNF304-integrin axis protects against anoikis in cancer
  JOURNAL   Nat Commun 6, 7351 (2015)
   PUBMED   26081979
  REMARK    GeneRIF: transcriptional regulator of beta1 integrin, promotes
            cancer cell survival and protects against anoikis in ovarian cancer
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4423)
  AUTHORS   Serra,R.W., Fang,M., Park,S.M., Hutchinson,L. and Green,M.R.
  TITLE     A KRAS-directed transcriptional silencing pathway that mediates the
            CpG island methylator phenotype
  JOURNAL   Elife 3, e02313 (2014)
   PUBMED   24623306
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4423)
  AUTHORS   Sabater,L., Ashhab,Y., Caro,P., Kolkowski,E.C., Pujol-Borrell,R.
            and Dominguez,O.
  TITLE     Identification of a KRAB-containing zinc finger protein, ZNF304, by
            AU-motif-directed display method and initial characterization in
            lymphocyte activation
  JOURNAL   Biochem Biophys Res Commun 293 (3), 1066-1072 (2002)
   PUBMED   12051768
  REMARK    GeneRIF: Identification of ZNF304, by AU-motif-directed display
            method and characterization of its role in lymphocyte activation
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005261.2, BP315423.1 and
            BC111532.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_020657.3.
            
            Summary: This gene encodes a member of the Krueppel C2H2-type
            zinc-finger family of proteins. The encoded protein functions as a
            transcriptional repressor that recruits a corepressor complex to
            stimulate promoter hypermethylation and transcriptional silencing
            of target genes. Expression of this gene is upregulated in
            colorectal, ovarian and breast cancer, and this gene may promote
            cancer cell survival, growth and invasion. [provided by RefSeq, Jul
            2016].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region, compared to variant 1. It encodes
            isoform 2, which is shorter than isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853564.14894.1,
                                           SRR14038191.2821539.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000282286.6/ ENSP00000282286.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC005261.2         179047-179049
            4-55                BP315423.1         1-52
            56-554              BC111532.1         1-499
            555-1492            BC111532.1         641-1578
            1493-4423           AC005261.2         184744-187674
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.43"
     gene            1..4423
                     /gene="ZNF304"
                     /note="zinc finger protein 304"
                     /db_xref="GeneID:57343"
                     /db_xref="HGNC:HGNC:13505"
                     /db_xref="MIM:613840"
     exon            1..427
                     /gene="ZNF304"
                     /inference="alignment:Splign:2.1.0"
     CDS             395..2374
                     /gene="ZNF304"
                     /note="isoform 2 is encoded by transcript variant 2;
                     KRAB-containing zinc finger protein"
                     /codon_start=1
                     /product="zinc finger protein 304 isoform 2"
                     /protein_id="NP_065708.2"
                     /db_xref="CCDS:CCDS12950.1"
                     /db_xref="GeneID:57343"
                     /db_xref="HGNC:HGNC:13505"
                     /db_xref="MIM:613840"
                     /translation="MAAAVLMDRVQSCVTFEDVFVYFSREEWELLEEAQRFLYRDVML
                     ENFALVATLGFWCEAEHEAPSEQSVSVEGVSQVRTAESGLFQKAHPCEMCDPLLKDIL
                     HLAEHQGSHLTQKLCTRGLCRRRFSFSANFYQHQKQHNGENCFRGDDGGASFVKSCTV
                     HMLGRSFTCREEGMDLPDSSGLFQHQTTYNRVSPCRRTECMESFPHSSSLRQHQGDYD
                     GQMLFSCGDEGKAFLDTFTLLDSQMTHAEVRPFRCLPCGNVFKEKSALINHRKIHSGE
                     ISHVCKECGKAFIHLHHLKMHQKFHTGKRHYTCSECGKAFSRKDTLVQHQRVHTGERS
                     YDCSECGKAYSRSSHLVQHQRIHTGERPYKCNKCGKAFSRKDTLVQHQRFHTGERPYE
                     CSECGKFFSQSSHLIEHWRIHTGARPYECIECGKFFSHNSSLIKHRRVHTGARSYVCS
                     KCGKAFGCKDTLVQHQIIHTGARPYECSECGKAFSRKDTLVQHQKIHTGERPYECGEC
                     GKFFSHSSNLIVHQRIHTGAKPYECNECGKCFSHNSSLILHQRVHTGARPYVCSECGK
                     AYISSSHLVQHKKVHTGARPYECSECGKFFSRNSGLILHQRVHTGEKPYVCSECGKAY
                     SRSSHLVRHQKAHTGERAHECNSFGGPLAASLKLV"
     exon            428..554
                     /gene="ZNF304"
                     /inference="alignment:Splign:2.1.0"
     exon            555..4423
                     /gene="ZNF304"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4396..4401
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF304"
                     /note="hexamer: AATAAA"
     polyA_site      4423
                     /gene="ZNF304"
                     /note="major polyA site"
ORIGIN      
        1 gtatttttcc tatgcccggt ccgtgcattc tggttgtgaa ggctgagttc tagagatcgg
       61 gtcggctttc tacgcggctc tcgtggaacc tagcaaagaa agacagtgaa gactgcagga
      121 ccttccttcg cgcttttgtt acaatccatg acccctgtcg tgggacgggc ggcctctcgc
      181 ggaggtgtct gccggggctg ggctcttacc gaggcctcca cacacgtcct cttgtccttg
      241 tctcccccag aagcagccgc cttagtcttg tgagcgtttt tacaccgggt agattgagac
      301 ttggagtgct acactcagcc cgagggcgtc cagcgcggtg gaggcgtggg gtttcggctg
      361 agcccacagg gcacagactg ttcatccgct tctcatggca gcggcggtgc tgatggaccg
      421 ggttcagagt tgtgtgacct tcgaggatgt gttcgtgtac ttctctcggg aggagtggga
      481 actccttgag gaggcacaga gattcctgta ccgtgatgtg atgctggaga actttgcact
      541 tgtggctaca ctaggttttt ggtgtgaagc agaacatgag gcaccttctg agcagagcgt
      601 ttctgtagaa ggagtgtcac aggtcaggac tgctgagtca ggtcttttcc agaaagcaca
      661 cccatgtgag atgtgtgacc cactcttgaa agacattttg cacctggctg aacaccaggg
      721 atcacacctt acacagaaac tgtgcacacg tgggctgtgt aggagaagat tctcgttcag
      781 tgcaaacttt taccagcacc agaagcaaca taatggagag aattgcttca gaggggatga
      841 tggaggggcc tcatttgtga agagctgtac agtccacatg ttagggagat cctttacgtg
      901 cagggaggaa gggatggact taccagatag ctctggcctt ttccagcacc agaccactta
      961 caatagggtg agtccatgca gaaggactga atgcatggag tctttcccac acagctccag
     1021 tctcaggcaa caccaaggag actatgatgg acagatgctt ttcagttgcg gtgatgaagg
     1081 gaaagccttc ctggacacct ttactcttct tgacagccag atgactcatg ctgaggtgag
     1141 acccttcaga tgcctaccat gtggaaatgt gttcaaggag aaatcagctc ttattaatca
     1201 cagaaaaatc cacagtggag aaatatctca tgtgtgtaag gagtgtggaa aagccttcat
     1261 tcacttgcac cacctaaaaa tgcaccagaa atttcacact ggaaaaagac actatacatg
     1321 cagtgaatgt gggaaggcct tcagccgcaa ggacacactt gttcagcatc agagagttca
     1381 cactggagaa agatcttatg actgcagtga atgtggaaaa gcctacagca gaagctccca
     1441 ccttgttcag caccagagaa ttcacacagg agaaaggcct tataagtgca acaaatgtgg
     1501 gaaagccttt agccgtaaag acacacttgt tcagcaccag agatttcata ctggagaaag
     1561 gccttatgag tgcagtgaat gtggaaaatt ctttagccaa agctcccacc ttattgagca
     1621 ctggagaatt cataccgggg caaggcccta tgaatgcata gaatgtggaa aattctttag
     1681 ccataactct agcctcatta aacatcggag agtccacaca ggagcaagat cctacgtgtg
     1741 cagcaaatgt gggaaggcct ttggctgcaa agacacactt gttcagcacc agataattca
     1801 cactggagca aggccttatg agtgcagtga atgtgggaag gccttcagcc gtaaagacac
     1861 acttgtgcaa caccaaaaaa tccacactgg agaaaggcct tatgagtgtg gtgaatgtgg
     1921 taaattcttc agccatagct ccaaccttat tgtacaccag agaattcaca ctggagcaaa
     1981 gccttatgag tgcaatgaat gtgggaaatg ctttagccac aactccagcc tcattttgca
     2041 ccagagagtt cacacaggag caaggcctta tgtgtgcagt gaatgtggga aggcttacat
     2101 tagtagctcc caccttgttc aacacaagaa agttcacact ggagcaagac cttatgagtg
     2161 cagtgaatgt gggaaattct ttagccgcaa ctctggcctc attctgcacc agagggttca
     2221 cactggagaa aagccttacg tatgcagcga atgtgggaaa gcctatagca gaagctccca
     2281 tcttgttcgt caccagaaag ctcacactgg agaaagagct cacgagtgca acagttttgg
     2341 tggcccttta gctgcatctc ttaaacttgt ttaacaccag aaaattcaca caagagaaag
     2401 gccttatgaa tgcagaaaat atgtcatctt gttcatcctc ataggactca caccagagca
     2461 atgctctgtg agtacccttt gtgagggaac catcagctag cagatgagca ccgtatattc
     2521 attccaccct ggggagattc ctgataagca ccacatatgt gggaggcttt catgaggtgt
     2581 gttgcacttt gtaactgtct agagctcttg atggaattat atcactgcca gtgcctgtgg
     2641 cggaagccat cttattgcta ccagctgtgt gtgtcaatca ctccattttg ctcagggaag
     2701 gcagacttct gtgctttctt tcctgttccc tacaggtaat catgaatatt ttcaaggact
     2761 tccccccccc cccacttcac cccctaccat tgagggtcct catcttttcc ctcatgatta
     2821 ggttctgagc aaacatgatc tagctctcac caaaaggacc tgagctaggg tctgctggga
     2881 tttcctgaca cgattttcca tcttcatgga caatgttaac tgtaaacgtg atagctgtga
     2941 cttacttgtc ttactgccaa atcgcccaaa tttggaactg cttgtcccat gctgctctga
     3001 tttatacagt gataagggcc tattgtggca gtctttactc ttggggattt tgttactgtg
     3061 tagagtggat tgagaaaaga attgggttct gtcatgaagg gagtagcacc ttttgtgggc
     3121 accaccttta tgtgcctcag aggggaccaa aggatggcag aaaactgttc tcagtctagt
     3181 ttgacctaat ttacactatt gccctaggct tgctaggaaa gactgaaaaa atttttgtgc
     3241 ctaactttgt ggcctggctg ccaggatttc ctgtgagccc agtgaggagg gcatagttgg
     3301 tgcgaaaatc tcctgtgctt gtggaagcaa cataagttgg gtcccttaac tgtcacgtac
     3361 tccccagcac tgttgaggga ttgctgtctt ctgtacctgt acaggatcaa gtccctgata
     3421 tccagaaccc cataggcaag taacattgac tgtaagacct ctgtagggca atgtgaaaat
     3481 catgattgct acagaagcac tgagataatg gagtggggat gactgtggca aacaaaagga
     3541 gattcgaaca ttctagaggg gcatccacaa gtctcggtgc agttatgatg gtgtgggatg
     3601 ggagtgcata gaaattctca ggaccttcag acatctgtat ctcctgtggc caaggccact
     3661 gtcagaggaa ataatctaaa tgtttgtgga ttcctgtgtc tccctgggca gttgacctgc
     3721 acagacagga acctcagcag tgacagaaga gagatccagg gatgggtctt ctctgaccca
     3781 gccagcattc cgagtgactg aggtggatgt ggacatcatt gcttaccaat ttttaccaac
     3841 attgaggcag ggctcactct cctaaattgt agggagagga atatggtaaa gccaaaatag
     3901 ttgacagatc taactttcct agtggaacag tatatataga ttttaatgag gaacatttat
     3961 ttaatagctt tatggaggta tgattaacat gcaataaact gcaaatatgt aaagtgtaaa
     4021 atgtgctgtt tcagcatgtg tgtacaccta tgaaaccacc acagtcaaga tatccaacac
     4081 aacaaaagat tgtcccttta taatcctcaa tttttcctta tcttgttttc cacaattcac
     4141 aagcaacagc aagcattttc tataatttta taaatgaaac catatggtgt gtacttttta
     4201 gggggtgggg ctctggcttt ttcagtaaac ataactattt taaggttaat ccattatctt
     4261 gttgcatgaa tcaattgttt gctcatttgt attgctgcgt agtatttcag tatttcattg
     4321 tataccacaa tttttcttct ttgtcaactt aattgttatg gatgtttggg taactttcag
     4381 tttttggcta ttacaaataa acctctgaag atttgtgtgc aaa
//
