LOCUS       NM_001109689            6337 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens zinc finger protein 250 (ZNF250), transcript variant
            2, mRNA.
ACCESSION   NM_001109689
VERSION     NM_001109689.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Yin,Y., Morgunova,E., Jolma,A., Kaasinen,E., Sahu,B.,
            Khund-Sayeed,S., Das,P.K., Kivioja,T., Dave,K., Zhong,F.,
            Nitta,K.R., Taipale,M., Popov,A., Ginno,P.A., Domcke,S., Yan,J.,
            Schubeler,D., Vinson,C. and Taipale,J.
  TITLE     Impact of cytosine methylation on DNA binding specificities of
            human transcription factors
  JOURNAL   Science 356 (6337) (2017)
   PUBMED   28473536
REFERENCE   3  (bases 1 to 6337)
  AUTHORS   Yachie,N., Petsalaki,E., Mellor,J.C., Weile,J., Jacob,Y., Verby,M.,
            Ozturk,S.B., Li,S., Cote,A.G., Mosca,R., Knapp,J.J., Ko,M., Yu,A.,
            Gebbia,M., Sahni,N., Yi,S., Tyagi,T., Sheykhkarimli,D., Roth,J.F.,
            Wong,C., Musa,L., Snider,J., Liu,Y.C., Yu,H., Braun,P.,
            Stagljar,I., Hao,T., Calderwood,M.A., Pelletier,L., Aloy,P.,
            Hill,D.E., Vidal,M. and Roth,F.P.
  TITLE     Pooled-matrix protein interaction screens using Barcode Fusion
            Genetics
  JOURNAL   Mol Syst Biol 12 (4), 863 (2016)
   PUBMED   27107012
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6337)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   5  (bases 1 to 6337)
  AUTHORS   Lorenz,P., Dietmann,S., Wilhelm,T., Koczan,D., Autran,S., Gad,S.,
            Wen,G., Ding,G., Li,Y., Rousseau-Merck,M.F. and Thiesen,H.J.
  TITLE     The ancient mammalian KRAB zinc finger gene cluster on human
            chromosome 8q24.3 illustrates principles of C2H2 zinc finger
            evolution associated with unique expression profiles in human
            tissues
  JOURNAL   BMC Genomics 11, 206 (2010)
   PUBMED   20346131
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6337)
  AUTHORS   Rual,J.F., Hirozane-Kishikawa,T., Hao,T., Bertin,N., Li,S.,
            Dricot,A., Li,N., Rosenberg,J., Lamesch,P., Vidalain,P.O.,
            Clingingsmith,T.R., Hartley,J.L., Esposito,D., Cheo,D., Moore,T.,
            Simmons,B., Sequerra,R., Bosak,S., Doucette-Stamm,L., Le Peuch,C.,
            Vandenhaute,J., Cusick,M.E., Albala,J.S., Hill,D.E. and Vidal,M.
  TITLE     Human ORFeome version 1.1: a platform for reverse proteomics
  JOURNAL   Genome Res 14 (10B), 2128-2135 (2004)
   PUBMED   15489335
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK054888.1, AB208810.1, DB272357.1, AF235103.5 and CA422363.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001109689.3.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1,
            resulting in a shorter protein (isoform b).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.3123584.1,
                                           SRR14038197.611532.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000417550.7/ ENSP00000393442.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AK054888.1         9-30
            23-228              AB208810.1         1-206
            229-229             DB272357.1         213-213
            230-2153            AB208810.1         208-2131
            2154-5832           AF235103.5         180424-184102       c
            5833-6337           CA422363.1         23-527              c
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..6337
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /note="zinc finger protein 250"
                     /db_xref="GeneID:58500"
                     /db_xref="HGNC:HGNC:13044"
     exon            1..55
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /inference="alignment:Splign:2.1.0"
     exon            56..151
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..1777
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /note="isoform b is encoded by transcript variant 2; zinc
                     finger protein 647; zinc finger protein (clone 647)"
                     /codon_start=1
                     /product="zinc finger protein 250 isoform b"
                     /protein_id="NP_001103159.1"
                     /db_xref="CCDS:CCDS55282.1"
                     /db_xref="GeneID:58500"
                     /db_xref="HGNC:HGNC:13044"
                     /translation="MAAARLLPVPAGPQAKLTFEDVAVLLSQDEWDRLCPAQRGLYRN
                     VMMETYGNVVSLGLPGSKPDIISQLERGEDPWVLDRKGAKKSQGLWSDYSDNLKYDHT
                     TACTQQDSLSCPWECETKGESQNTDLSPKPLISEQTVILGKTPLGRIDQENNETKQSF
                     CLSPNSVDHREVQVLSQSMPLTPHQAVPSGERPYMCVECGKCFGRSSHLLQHQRIHTG
                     EKPYVCSVCGKAFSQSSVLSKHRRIHTGEKPYECNECGKAFRVSSDLAQHHKIHTGEK
                     PHECLECRKAFTQLSHLIQHQRIHTGERPYVCPLCGKAFNHSTVLRSHQRVHTGEKPH
                     RCNECGKTFSVKRTLLQHQRIHTGEKPYTCSECGKAFSDRSVLIQHHNVHTGEKPYEC
                     SECGKTFSHRSTLMNHERIHTEEKPYACYECGKAFVQHSHLIQHQRVHTGEKPYVCGE
                     CGHAFSARRSLIQHERIHTGEKPFQCTECGKAFSLKATLIVHLRTHTGEKPYECNSCG
                     KAFSQYSVLIQHQRIHTGEKPYECGECGRAFNQHGHLIQHQKVHRKL"
     exon            152..278
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /inference="alignment:Splign:2.1.0"
     exon            279..392
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /inference="alignment:Splign:2.1.0"
     exon            393..455
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /inference="alignment:Splign:2.1.0"
     exon            456..6337
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2103..2108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /note="hexamer: AATAAA"
     regulatory      2122..2127
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /note="hexamer: AATAAA"
     polyA_site      2127
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /note="major polyA site"
     polyA_site      2148
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /note="major polyA site"
     regulatory      6316..6321
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /note="hexamer: AATAAA"
     polyA_site      6337
                     /gene="ZNF250"
                     /gene_synonym="ZFP647; ZNF647"
                     /note="major polyA site"
ORIGIN      
        1 gtctgaaggt gctgcgggat gccgttcctt cgcgcgtgag gctgcggctc tgacgcccca
       61 taggctcctt caatttccgt gatcctcgga gtccccagga gaccaggtga tggcagcagc
      121 cagactcctg ccagtgccgg caggacccca ggccaagctg accttcgagg atgtggctgt
      181 gctcctctcc caggatgaat gggaccgcct gtgccctgct cagaggggtc tctacagaaa
      241 tgtgatgatg gaaacctatg ggaatgtagt ctcattggga cttccaggat ccaagcctga
      301 cataatctcc cagctggagc gaggggaaga tccctgggtc ctggacagga agggggctaa
      361 gaagagccag ggcctgtgga gtgactactc agacaacctc aaatatgacc acactacagc
      421 ctgtacacaa caagacagtt tatcttgtcc atgggaatgt gaaaccaagg gagagagtca
      481 aaatacagac ttgagtccga agccattaat ttcagagcaa acagtgattc tggggaaaac
      541 acccttgggg aggattgatc aagaaaataa tgaaacaaag caaagcttct gtctgagtcc
      601 aaactctgtt gaccaccgtg aagttcaggt cttaagccaa agcatgccac tcactccgca
      661 ccaggcagtg cctagtggag agaggcccta catgtgtgtt gagtgtggga agtgctttgg
      721 ccggagttcc cacctccttc agcatcagcg tatccacact ggagagaagc cctatgtgtg
      781 cagtgtatgt gggaaggcct tcagccagag ctcagtcctt agtaaacaca ggagaattca
      841 cacaggtgag aagccctatg agtgtaatga gtgtggaaaa gcctttagag tgagctcaga
      901 tcttgctcag catcacaaga tacatacagg agagaagcct cacgaatgtc ttgagtgtcg
      961 gaaagccttc actcaactct cacatctcat tcagcaccag cggatccaca cgggagaaag
     1021 gccatatgtg tgtccgttgt gtgggaaagc cttcaaccat agcactgttc tgcggagcca
     1081 ccagagggta cacactgggg agaagcctca caggtgcaat gagtgtggga aaaccttcag
     1141 tgtgaagagg acactgctgc agcaccagag gatccacacc ggggagaagc cctacacgtg
     1201 cagcgagtgt gggaaggcct tcagcgaccg ctcagtcctc attcagcacc acaacgtgca
     1261 caccggggag aagccctatg agtgcagtga gtgtgggaag accttcagcc accgctccac
     1321 actgatgaat cacgagcgga tccacaccga ggaaaagccc tatgcatgct acgaatgtgg
     1381 gaaggccttc gttcagcact cacacctgat ccagcaccag agagtccaca ctggggagaa
     1441 gccctatgtg tgtggtgaat gtgggcacgc cttcagtgca cgccggtctc tgatccagca
     1501 tgagagaatc cacacaggtg aaaagccctt ccagtgcaca gaatgtggca aagccttcag
     1561 cctgaaagca actctgattg tgcacctgag gacccacacg ggcgagaagc catatgagtg
     1621 caatagctgc gggaaggcct tcagccagta ctcagtgctc atccagcacc agcggatcca
     1681 cacaggcgag aagccctatg agtgcgggga gtgtgggcgt gccttcaacc agcatggcca
     1741 cctaatccag caccagaaag tgcacagaaa gttgtgaccc atggctgaca caagaatcca
     1801 ttctcacaga aactgcatgt ggaaccacaa gcagccttca gcccaagaga agtctctgtt
     1861 aactctatag gaagcttttc tttggcgatt cagtgtcaca aaataactcc agaaagaagc
     1921 acttagcgtg ctgttcctgt ggaaaaactt cagagactac ctgttttatt ttcctcaaca
     1981 tcttgaagtt atgttggaga gtaatcatac aattgtagag aattttggta aaaaacagcc
     2041 ataattcttt aacattagtt tatttgaact aagggaattt aaggcataag aaccattatc
     2101 ccaataaaat cttacattcc aaataaagtt ctttttctaa gaacattaca tgcccttcct
     2161 aaatatcaat taaccatact aattattgca cttaaaattt gaaagtcgga ctattttcag
     2221 tattctctta aaagactaaa gtattgtatg gataaagtga tataaaaaga tattttcatc
     2281 aagcatcatg taaaatggtt ggaaatccta agtgtgtgga tttccatcct aagtgtgtgg
     2341 agtcctgttt tgtatgaaac aaggaaaaag cttatatatt agtgagaaat tacataaatt
     2401 taaaaaactt tttgtatgtg tgtgtggaga tagtcttgct ctgttgccca agctgcagtg
     2461 cagtggcatg atcttggctc actgcagtct ccgcccccac cccaggttca agcttttctt
     2521 gtgcctcagt ctcccaagta actaggatta tagatatgcg ccaccacacc tggctaattt
     2581 ttgtattttt agtagagacg gagtttcacc gtgttggcca ggctggtctc gaactcctgg
     2641 cctcaagtga tcctcccacc tttgcttccg aaagtgttgg gattacaggc gtgaggcacc
     2701 atgcctggcc tagaaaaccc tttatctgga gcttttaact ttattcaaga tcagaaaggt
     2761 tatgctaact ttttagtaaa aattcaactc aaaaatgggt attttcctca aaacttgccc
     2821 ttatgacctg cagatcctgt gccccagaaa ccgtgagatg tctcaattcc catgcaggca
     2881 aaggagggga gcttcacact cagcagacct catagctgtt acaccaagtg ggtacctctt
     2941 tacatcttat ggtatttccc ccaaaacaat gctgaaaata ctgttctttg gtagatatat
     3001 ttaaaacata catttctcaa attcagatag ctctttttta ctatctctag tatttctatt
     3061 cctatatttg aaggggtttt ttttttcatt tttttactcc tggttcaaga gtccagtatc
     3121 cattgccata ttactatgat gaagatgaat ctagtttatt cataattaaa aatttgtatt
     3181 cagaattatt tgttctgttt taagcatact tttttttttt tttttgagac aaagtcttgc
     3241 tcttgttccc caggctgaag tgcaatggca caatctcagc tcactgcaat ctccacctcc
     3301 caggttcaag caattctcct gcctctgcct cccaagtagc tggaattaca ggcacctgct
     3361 accacgcccg actaattttc gtatttttag tagagatgag gtttcaccat gttggccagg
     3421 ctggtctcaa actcctgacc tcaggtgatc cgcccgcctc ggcctcccaa agtgctggga
     3481 ttacaggcgt gtgccaccgc acccggccaa ggatacagtt cttgaaaccg aatcagaacc
     3541 atttctagta gcagccagct gcaaacgaaa caaatgtatg aggttcaagc cacttgaaat
     3601 tctcaacatg ttaagtgcct gagatagaga agggctgtct tcaccccagg cccccgcccc
     3661 caacatctgt cagtttggtg gggaggaaat agtcaccagg atattgatcc tttttgtttg
     3721 ttttaaagac ggtcttgctc tgtcaccagg ttggagtgca gtggtgccat catggctcac
     3781 cgctgcctca aactcctggg ctgaagcagt cctcccacct caccttccca aaaggttagg
     3841 attacaggca tgagctgctg tgcccatcca ggcattgatc cttaagtcca cagcaaaaca
     3901 ccacgatgta gacaaaagct tgttccccct tctatgtaca gacactgaca acagtgaatt
     3961 attaactcca gagaggaact ggtaactgat tggttaactt gagaaaggag ctgacatcac
     4021 tggtgtttta cttcagagca actgaaacca ctgaagtgaa attccagagg aactaatatt
     4081 actgaggtgt tacttcagag ggactgagat cactgaagtg aaacctcaga tagctcagat
     4141 aaccaaggtg ttaaccttag aggtgttgcc attctgtgag gtgttgtttc agatagataa
     4201 aataactaag gtgttagttc agagaagaca agagcttaac tgtttctttg gagaagagct
     4261 gagatcactg aggcgtcact tcagaaagaa agtgagacaa tttttgttac ttcagaggat
     4321 ctgagtacac tgaggtgttt catcagagag gaggtgagat cactgggtgt tacttctgaa
     4381 aggaattgac atcactagtg ggtactgcag agtagctctg accactgagg tttacttcag
     4441 aggagctgaa ataacggaaa tgggatcact aaggtattac tttagagatt agatcattga
     4501 ggtaattctt cagagaagct gagatcacag aggtgtttct tcagagaagt tgaaatcact
     4561 aatgtgaaac tctagaagaa tggaggtcac tgagtgctat ttcataaagg aagtgaaatc
     4621 actatattgt accatcggaa agatgagaat gctgagatgt tacttaggag ctgagatcac
     4681 tgaggttctg cttcagagaa aagctgatac caatgagatg ttacttaagg gaggagctga
     4741 ggttactgag gtattattca ggaccaagat ggtgtccctc aggtgttaga agctgagatc
     4801 acagagatgt tacttaagag aggctgagac tgctgatgtg attcttcaga aaggaaatga
     4861 gatcactaag ctgttaactt tagaaaggag atgaaataac tgagttgttc actggagttg
     4921 agatcacaca ggtgttaact tcagaggtgg ctgaaatcac taaggtgatt cttcagagaa
     4981 aggttgagat cactaggggt gatgctttag aaagaggtta ctttagggaa aagctatgat
     5041 cactgagaag ttacttcaga atggagctga gatcactgag gtaacagtcc aaagaggaac
     5101 tgagattact gaggtgttac ttcagagcca agagatcacc caggtaccta gatgagatca
     5161 ctgagatgaa agtccagaaa cctgaggtca cctcaatatt aattgagaga aaatgagaac
     5221 actgtggtgc tatcttaaat cttctatgaa aggaagctgc caacctgtgt tagctgagtc
     5281 cacagctggc atgtgactca gtggcagctg ctcagtgccc actactattc aggccccaat
     5341 ttctaatcat taaagacaaa gtcatattcc aattaagatg agaataatta aattcaatat
     5401 acaggaacat ttgtttagaa agactgtgta tcacaggaca acgtaagtta cacagtacaa
     5461 gttattctgg attccaataa cctagggttt tcagggaaat ccttccaaaa agtatagttt
     5521 ttttcctgca ttcttaaaat gagcttaata tcattctgcc tgttcttgct atgtcaggct
     5581 gccattgatc cactggtgag aatgcaggct caggtgtcca cggagccagg aagtgggctt
     5641 caatgccctt ctttctgagg aggattccca ggctacattt tcttgccact cacctcctcc
     5701 tctccaagga gaaagaggtg gcacatgacc tactggaaca tgtgggctat ctaccttttt
     5761 gggatataac atcaaaggcc agggccctga attttcatgc tgataacagt cttctcaacc
     5821 attatcttct gcctgatttg tcagcagcat tccaccttta ttttggattt gttttattca
     5881 gaagtctgta tattttaggt atttaaatac ttctctttgg ctgggtgcaa tggctcacac
     5941 ctgtaatccc agcactttgg gaggctgaag tgggtggatc gcttgagccc aggactttga
     6001 gaccagcatg gcgaaaaccc gtctctacaa aaaaaaaatt agctgggtgt tgtggcttgc
     6061 gcctgtagtc ccagatacgt gggaggctaa ggtgggagga ttgattggga gttcaagact
     6121 agtgtgctgt gatcatacta tctagcctgg acaacagaga gagaccttgt cacaaaaaat
     6181 acgtatatat ttcttttaaa attgtttctt ctaacagcta ttaagaattt gaaacttggt
     6241 tctcttcaaa ttttctatta ttttggaaaa cattttagtc tgcctggaat ttctgtgtat
     6301 aatctgacgc tttccaataa aaaagtaatg actggaa
//
