LOCUS       NM_001321645            3993 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens zinc finger protein 224 (ZNF224), mRNA.
ACCESSION   NM_001321645 XM_005259221
VERSION     NM_001321645.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Cesaro,E., Pastore,A., Polverino,A., Manna,L., Divisato,G.,
            Quintavalle,C., Sanzo,M.D., Faniello,M.C., Grosso,M. and
            Costanzo,P.
  TITLE     ZNF224 is a mediator of TGF-beta pro-oncogenic function in melanoma
  JOURNAL   Hum Mol Genet 30 (22), 2100-2109 (2021)
   PUBMED   34181020
  REMARK    GeneRIF: ZNF224 is a mediator of TGF-beta pro-oncogenic function in
            melanoma.
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Cesaro,E., Lupo,A., Rapuano,R., Pastore,A., Grosso,M. and
            Costanzo,P.
  TITLE     ZNF224 Protein: Multifaceted Functions Based on Its Molecular
            Partners
  JOURNAL   Molecules 26 (20), 6296 (2021)
   PUBMED   34684876
  REMARK    GeneRIF: ZNF224 Protein: Multifaceted Functions Based on Its
            Molecular Partners.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3993)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3993)
  AUTHORS   Sodaro,G., Blasio,G., Fiorentino,F., Auberger,P., Costanzo,P. and
            Cesaro,E.
  TITLE     ZNF224 is a transcriptional repressor of AXL in chronic myeloid
            leukemia cells
  JOURNAL   Biochimie 154, 127-131 (2018)
   PUBMED   30176265
  REMARK    GeneRIF: identify the receptor tyrosine kinase Axl as a novel
            target of ZNF224 transcriptional repression activity.
REFERENCE   5  (bases 1 to 3993)
  AUTHORS   Cesaro,E., Sodaro,G., Montano,G., Grosso,M., Lupo,A. and
            Costanzo,P.
  TITLE     The Complex Role of the ZNF224 Transcription Factor in Cancer
  JOURNAL   Adv Protein Chem Struct Biol 107, 191-222 (2017)
   PUBMED   28215224
  REMARK    GeneRIF: Increasing information on the mechanism through which
            ZNF224 can operate could lead to the identification of agents
            capable of modulating ZNF224 function, thus potentially paving the
            way to new therapeutic strategies for treatment of cancer
            Review article
REFERENCE   6  (bases 1 to 3993)
  AUTHORS   Shannon,M., Hamilton,A.T., Gordon,L., Branscomb,E. and Stubbs,L.
  TITLE     Differential expansion of zinc-finger transcription factor loci in
            homologous human and mouse gene clusters
  JOURNAL   Genome Res 13 (6A), 1097-1110 (2003)
   PUBMED   12743021
REFERENCE   7  (bases 1 to 3993)
  AUTHORS   Medugno,L., Costanzo,P., Lupo,A., Monti,M., Florio,F., Pucci,P. and
            Izzo,P.
  TITLE     A novel zinc finger transcriptional repressor, ZNF224, interacts
            with the negative regulatory element (AldA-NRE) and inhibits gene
            expression
  JOURNAL   FEBS Lett 534 (1-3), 93-100 (2003)
   PUBMED   12527367
  REMARK    GeneRIF: We demonstrate that ZNF224, a Kruppel-like zinc finger
            transcription factor, is the repressor protein that specifically
            binds to the negative cis-element AldA-NRE and affects the
            AldA-NRE-mediated transcription
REFERENCE   8  (bases 1 to 3993)
  AUTHORS   Lee,T.H., Lwu,S., Kim,J. and Pelletier,J.
  TITLE     Inhibition of Wilms tumor 1 transactivation by bone marrow zinc
            finger 2, a novel transcriptional repressor
  JOURNAL   J Biol Chem 277 (47), 44826-44837 (2002)
   PUBMED   12239212
  REMARK    GeneRIF: Results suggest that bone marrow zinc finger 2 (BMZF2)
            interferes with the transactivation potential of Wilms tumor
            suppressor gene (WT1).
REFERENCE   9  (bases 1 to 3993)
  AUTHORS   Han,Z.G., Zhang,Q.H., Ye,M., Kan,L.X., Gu,B.W., He,K.L., Shi,S.L.,
            Zhou,J., Fu,G., Mao,M., Chen,S.J., Yu,L. and Chen,Z.
  TITLE     Molecular cloning of six novel Kruppel-like zinc finger genes from
            hematopoietic cells and identification of a novel transregulatory
            domain KRNB
  JOURNAL   J Biol Chem 274 (50), 35741-35748 (1999)
   PUBMED   10585455
REFERENCE   10 (bases 1 to 3993)
  AUTHORS   Thiesen,H.J.
  TITLE     Multiple genes encoding zinc finger domains are expressed in human
            T cells
  JOURNAL   New Biol 2 (4), 363-374 (1990)
   PUBMED   2288909
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA245440.1, DB228337.1,
            CN304667.1, AC114266.2, AC021092.1 and AA554187.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 13, 2020 this sequence version replaced NM_001321645.2.
            
            Summary: This gene encodes a member of the Krueppel C2H2-type
            zinc-finger family of proteins. The encoded protein represses
            transcription of the aldolase A gene, which encodes a key enzyme in
            glycolysis. The encoded zinc-finger protein may also function as a
            transcriptional co-activator with Wilms' tumor protein 1 to
            regulate apoptotic genes in leukemia. [provided by RefSeq, Jul
            2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853565.15486.1,
                                           SRR11853563.17124.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000693561.1/ ENSP00000508532.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               DA245440.1         33-160
            129-144             DB228337.1         151-166
            145-650             CN304667.1         1-506
            651-3097            AC114266.2         641-3087
            3098-3658           AC021092.1         1448-2008
            3659-3993           AA554187.1         11-345              c
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.31"
     gene            1..3993
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /note="zinc finger protein 224"
                     /db_xref="GeneID:7767"
                     /db_xref="HGNC:HGNC:13017"
                     /db_xref="MIM:194555"
     exon            1..170
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /inference="alignment:Splign:2.1.0"
     exon            171..259
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /inference="alignment:Splign:2.1.0"
     exon            260..342
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /inference="alignment:Splign:2.1.0"
     CDS             328..2451
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /note="zinc finger protein 255; zinc finger protein 233;
                     bone marrow zinc finger 2; zinc finger protein 27; zinc
                     finger protein KOX22"
                     /codon_start=1
                     /product="zinc finger protein 224"
                     /protein_id="NP_001308574.1"
                     /db_xref="CCDS:CCDS33046.1"
                     /db_xref="GeneID:7767"
                     /db_xref="HGNC:HGNC:13017"
                     /db_xref="MIM:194555"
                     /translation="MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNL
                     LSVGHQAFHRDTFHFLREEKIWMMKTAIQREGNSGDKIQTEMETVSEAGTHQEWSFQQ
                     IWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSVIHTRQKSSQGNGYKPSFSDV
                     SHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCGKEFSQSSH
                     LQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQ
                     EHQRIHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSH
                     RMIHTGEKPYKCEECGKGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQR
                     VHSGEKPFKCEECGKGFYTNSQCYSHQRSHSGEKPYKCVECGKGYKRRLDLDFHQRVH
                     TGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECGKRFTQNSHLHSHQRVHTG
                     EKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQRLHSGEK
                     PFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPL
                     KCEQHGKNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKC
                     RECDMCFSQASSLRLHQNVHVGEKP"
     exon            343..469
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /inference="alignment:Splign:2.1.0"
     exon            470..562
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /inference="alignment:Splign:2.1.0"
     exon            563..3993
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2466..2471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /note="hexamer: AATAAA"
     polyA_site      2493
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /note="major polyA site"
     regulatory      3509..3514
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /note="hexamer: TATAAA"
     polyA_site      3530
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
     regulatory      3971..3976
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /note="hexamer: AATAAA"
     polyA_site      3993
                     /gene="ZNF224"
                     /gene_synonym="BMZF-2; BMZF2; KOX22; ZNF233; ZNF255;
                     ZNF27"
                     /note="major polyA site"
ORIGIN      
        1 ggagtcgcgg acacttccgc tcggggactg aggttgctgc agtttttccg cgatagtttg
       61 gggcagttcc gcgcgttgca ggcccttctg aatttcctgg acctacgcat tggatcctca
      121 aagaactggt gatcgaatta ggggaaaagg ggcctcgtcg gagagtagag ctgaatacca
      181 ctagaaacat acctgtaacc agagacagct gattatagct ttctgcagca aggaagccca
      241 cgtaccaggg gctgtcttgg cacaattctg ctttcccagg aactgcatca ctcaggactc
      301 tgcaagtttc cagaagtaag agggaaaatg accacgttca aggaggcaat gaccttcaag
      361 gacgtggctg tggtcttcac tgaggaagag ctggggctgc tggaccttgc tcagaggaag
      421 ctgtatcgag atgtgatgct ggagaacttc aggaacctgc tctcagtggg acatcaagca
      481 ttccacaggg atactttcca cttcctaagg gaagaaaaga tttggatgat gaagacagca
      541 atccaaaggg aagggaattc aggagacaag atccaaactg agatggagac tgtttcagaa
      601 gcaggaacac atcaagagtg gtccttccag caaatctggg aaaaaattgc aagtgattta
      661 accaggtctc aagacttgat gataaatagc tctcagttct ccaaagaagg tgatttcccc
      721 tgccagactg aggcaggact atctgtaatt cacacaagac agaaatcttc ccagggcaat
      781 ggatataaac catccttcag tgatgtctcc cactttgatt ttcatcaaca attacactca
      841 ggagagaaat ctcatacgtg tgatgagtgt ggaaagaact tttgttacat ctcagccctt
      901 cgtattcatc agagagtcca catgggagag aaatgctata agtgtgacgt gtgtggtaag
      961 gaattcagtc agagttcaca tctgcaaact catcagagag tccacactgg agagaaaccg
     1021 ttcaaatgtg tggaatgtgg gaaaggcttc agtcgtagat cagcacttaa tgttcatcat
     1081 aaattacaca caggagagaa accttataat tgtgaggaat gcgggaaggc cttcattcac
     1141 gattcccagc ttcaagaaca tcagagaatc catacggggg agaagccatt caaatgtgat
     1201 atatgtggta agagcttctg tggtagatca agacttaata ggcattccat ggttcacacg
     1261 gcagagaaac cattccgatg tgatacgtgt gataagagct ttcgtcagag atcagcactt
     1321 aatagtcatc gcatgatcca cacaggagag aaaccataca aatgtgagga gtgtggaaaa
     1381 ggctttattt gtaggcgaga tctttatacg catcatatgg tccacacggg agaaaagcca
     1441 tataattgta aagagtgtgg gaagagcttc agatgggcct cgtgtctttt gaaacatcag
     1501 cgagtccaca gtggagaaaa accattcaaa tgtgaagaat gtgggaaagg attttacaca
     1561 aattcacaat gctattccca ccagagatcc catagtggag aaaaaccata caaatgtgtg
     1621 gagtgtggga agggctacaa aaggaggttg gatcttgact ttcaccagcg cgtccataca
     1681 ggagagaaac tgtataattg taaggaatgt gggaagagct ttagtcgggc cccatgtctt
     1741 ttgaaacatg agagactcca cagtggagaa aaaccattcc aatgtgaaga gtgtgggaag
     1801 agatttactc aaaattcaca tcttcattcc catcagagag ttcacactgg agaaaagcca
     1861 tacaaatgtg agaagtgtgg aaagggctac aatagtaagt ttaatcttga tatgcaccag
     1921 aaggtccaca caggagagag accatacaat tgtaaggaat gtgggaagag ttttggctgg
     1981 gcctcgtgtc ttttgaaaca tcagagactg cacagtggag aaaaaccatt caaatgtgaa
     2041 gagtgtggga aaagatttac tcagaattca cagcttcatt ctcatcaaag agtgcacact
     2101 ggagaaaagc catacaaatg tgatgagtgt gggaagggct tcagctggtc ctcaactcgt
     2161 ctgacccatc agagacgcca cagcagagaa acacctctca aatgtgagca gcatgggaag
     2221 aacattgtac agaattcatt ctctaaagtg caagaaaaag ttcacagtgt agaaaagcca
     2281 tacaaatgtg aggactgtgg gaagggctac aacaggcgct tgaatcttga tatgcatcag
     2341 agggtccaca tgggagagaa aacatggaag tgtagggagt gtgatatgtg ctttagtcag
     2401 gcctcaagcc ttcgacttca tcagaatgtt catgttggag aaaaacctta gtgatgtgat
     2461 ggtgcaataa agtcttcact cagtcttcat gaatgcagtc tcatctgaaa gttcacaaag
     2521 gagagacaca ttaaaatatg aggcacatag taagcacttc cctttgagta ttttatctct
     2581 gaatccatgc tggtgataaa tttcacccat tcttggaaga gggaaaacat ttgtttaaga
     2641 taacatcagt gcttcagcca tagctcagca tgccccagta gtctcaggac caccataatg
     2701 gagaatcctt gtaaatggtg caataaagct tcattcagaa gtttgataat tacagctatc
     2761 attcaggaga caggccttag aaagagtaag agttcatgaa tttaccagac taatggtgga
     2821 catgtccaaa ttgatgtcca ctagaatgta aactacatgt gtctttgctg ctaagtcgtc
     2881 acagccttaa cattgattag cactttgtat gtgttcagta cttgtttgtt aaatcaatga
     2941 aaggataaaa catatgtatg gatttggata caattttgtg tatccaaaaa gggaagcctg
     3001 caaaaaataa ttctgggaaa catgagttga aatgcagagt aaactcagta ctgcaatcca
     3061 tagcaatatt ttgcatatta ttattttttc ttttaaattt ttttcttgat cagatttatc
     3121 atattcattg aacattattt tattgcactt ggctcctggc ttccccccat cctgtgctct
     3181 gaagtcagag tattatttgt ggctctatga aatgtccatt gcttgtatac ggtataaatt
     3241 taattatctt ttgtagtttt tctcacagta tagactgaaa aggtttggtt aattttgcaa
     3301 taacagacca ctgcatattt cacaattaag tgttttcaac agtattaagg caagatttac
     3361 agtaacactt ttaaagtgcc agatgtacat tgacagaaat tttcttagtc aatcctagat
     3421 tgatcatgat ttgtgatttc tatgcaggcc ttggtatgat tgccttttaa ttgctgtaca
     3481 atttcttttt tttttttaac ttctctggta taaaatattc taaatttgaa tgacagcttt
     3541 tgtcaacctt cttatccaat attcttccac caacgctatg aaacaggaag agaaaatgga
     3601 gtttggtaat ttcatcaaat atattcatta ttgggggcag gagtaacatt taaatcctcc
     3661 tctttattgc tggcagtaaa aatatgtctt ttggagagca gaacatgcca gaatgtataa
     3721 ggtgcaactg tttgatatga aaaccatagg ataggaaact atgcctatga cactaaaact
     3781 ccaaaaagta gaagaccaca ttgatgatgt cactgcattt taaaatggaa agaacaagtg
     3841 ttcaagaaca ggaatttgtg tgaattgcat ttattcctat aataatatga actcaatgag
     3901 atgatgacat agacacaata acaaaaggag aaaaacaatg tggctttttt tagccttcat
     3961 aacctaatca aataaatatc tttgtgtgtt tta
//
