LOCUS       NM_003450               2459 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens zinc finger protein 174 (ZNF174), transcript variant
            1, mRNA.
ACCESSION   NM_003450
VERSION     NM_003450.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2459)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 2459)
  AUTHORS   Yin,Y., Morgunova,E., Jolma,A., Kaasinen,E., Sahu,B.,
            Khund-Sayeed,S., Das,P.K., Kivioja,T., Dave,K., Zhong,F.,
            Nitta,K.R., Taipale,M., Popov,A., Ginno,P.A., Domcke,S., Yan,J.,
            Schubeler,D., Vinson,C. and Taipale,J.
  TITLE     Impact of cytosine methylation on DNA binding specificities of
            human transcription factors
  JOURNAL   Science 356 (6337) (2017)
   PUBMED   28473536
REFERENCE   5  (bases 1 to 2459)
  AUTHORS   Ravasi,T., Suzuki,H., Cannistraci,C.V., Katayama,S., Bajic,V.B.,
            Tan,K., Akalin,A., Schmeier,S., Kanamori-Katayama,M., Bertin,N.,
            Carninci,P., Daub,C.O., Forrest,A.R., Gough,J., Grimmond,S.,
            Han,J.H., Hashimoto,T., Hide,W., Hofmann,O., Kamburov,A., Kaur,M.,
            Kawaji,H., Kubosaki,A., Lassmann,T., van Nimwegen,E.,
            MacPherson,C.R., Ogawa,C., Radovanovic,A., Schwartz,A.,
            Teasdale,R.D., Tegner,J., Lenhard,B., Teichmann,S.A., Arakawa,T.,
            Ninomiya,N., Murakami,K., Tagami,M., Fukuda,S., Imamura,K., Kai,C.,
            Ishihara,R., Kitazume,Y., Kawai,J., Hume,D.A., Ideker,T. and
            Hayashizaki,Y.
  TITLE     An atlas of combinatorial transcriptional regulation in mouse and
            man
  JOURNAL   Cell 140 (5), 744-752 (2010)
   PUBMED   20211142
  REMARK    Erratum:[Cell. 2010 Apr 16;141(2):369. Kamburov, Atanas [added];
            Kaur, Mandeep [added]; MacPherson, Cameron Ross [added];
            Radovanovic, Aleksandar [added]; Schwartz, Ariel [added]]
REFERENCE   6  (bases 1 to 2459)
  AUTHORS   Ivanov,D., Stone,J.R., Maki,J.L., Collins,T. and Wagner,G.
  TITLE     Mammalian SCAN domain dimer is a domain-swapped homolog of the HIV
            capsid C-terminal domain
  JOURNAL   Mol Cell 17 (1), 137-143 (2005)
   PUBMED   15629724
REFERENCE   7  (bases 1 to 2459)
  AUTHORS   Rush,J., Moritz,A., Lee,K.A., Guo,A., Goss,V.L., Spek,E.J.,
            Zhang,H., Zha,X.M., Polakiewicz,R.D. and Comb,M.J.
  TITLE     Immunoaffinity profiling of tyrosine phosphorylation in cancer
            cells
  JOURNAL   Nat Biotechnol 23 (1), 94-101 (2005)
   PUBMED   15592455
REFERENCE   8  (bases 1 to 2459)
  AUTHORS   Stone,J.R., Maki,J.L., Blacklow,S.C. and Collins,T.
  TITLE     The SCAN domain of ZNF174 is a dimer
  JOURNAL   J Biol Chem 277 (7), 5448-5452 (2002)
   PUBMED   11741982
  REMARK    GeneRIF: Both size exclusion chromatography and equilibrium
            sedimentation analysis demonstrate that the ZNF174 SCAN domain
            forms a homodimer
REFERENCE   9  (bases 1 to 2459)
  AUTHORS   Williams,A.J., Blacklow,S.C. and Collins,T.
  TITLE     The zinc finger-associated SCAN box is a conserved oligomerization
            domain
  JOURNAL   Mol Cell Biol 19 (12), 8526-8535 (1999)
   PUBMED   10567577
REFERENCE   10 (bases 1 to 2459)
  AUTHORS   Williams,A.J., Khachigian,L.M., Shows,T. and Collins,T.
  TITLE     Isolation and characterization of a novel zinc-finger protein with
            transcription repressor activity
  JOURNAL   J Biol Chem 270 (38), 22143-22152 (1995)
   PUBMED   7673192
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA962432.1, BI918471.1,
            AC025283.6, U31248.1 and BM969505.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003450.2.
            
            Summary: This gene encodes a protein with three Cys2-His2-type zinc
            fingers in the carboxy-terminus, a putative nuclear localization
            signal, and an amino-terminus SCAN box which forms homodimers. This
            protein is believed to function as a transcriptional repressor.
            Alternative splicing results in multiple transcript variants
            encoding distinct isoforms. [provided by RefSeq, Dec 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.800895.1,
                                           SRR1660807.253113.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000268655.5/ ENSP00000268655.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-494               DA962432.1         26-519
            495-741             BI918471.1         507-753
            742-1192            AC025283.6         99525-99975
            1193-1415           AC025283.6         101995-102217
            1416-2072           U31248.1           1211-1867
            2073-2459           BM969505.1         15-401              c
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..2459
                     /gene="ZNF174"
                     /gene_synonym="ZSCAN8"
                     /note="zinc finger protein 174"
                     /db_xref="GeneID:7727"
                     /db_xref="HGNC:HGNC:12963"
                     /db_xref="MIM:603900"
     exon            1..1192
                     /gene="ZNF174"
                     /gene_synonym="ZSCAN8"
                     /inference="alignment:Splign:2.1.0"
     CDS             791..2014
                     /gene="ZNF174"
                     /gene_synonym="ZSCAN8"
                     /note="isoform a is encoded by transcript variant 1; AW-1;
                     zinc finger and SCAN domain-containing protein 8"
                     /codon_start=1
                     /product="zinc finger protein 174 isoform a"
                     /protein_id="NP_003441.1"
                     /db_xref="CCDS:CCDS10504.1"
                     /db_xref="GeneID:7727"
                     /db_xref="HGNC:HGNC:12963"
                     /db_xref="MIM:603900"
                     /translation="MAAKMEITLSSNTEASSKQERHIIAKLEEKRGPPLQKNCPDPEL
                     CRQSFRRFCYQEVSGPQEALSQLRQLCRQWLQPELHTKEQILELLVMEQFLTILPPEI
                     QARVRHRCPMSSKEIVTLVEDFHRASKKPKQWVAVCMQGQKVLLEKTGSQLGEQELPD
                     FQPQTPRRDLRESSPAEPSQAGAYDRLSPHHWEKSPLLQEPTPKLAGTEAPRMRSDNK
                     ENPQQEGAKGAKPCAVSAGRSKGNGLQNPEPRGANMSEPRLSRRQVSSPNAQKPFAHY
                     QRHCRVEYISSPLKSHPLRELKKSKGGKRSLSNRLQHLGHQPTRSAKKPYKCDDCGKS
                     FTWNSELKRHKRVHTGERPYTCGECGNCFGRQSTLKLHQRIHTGEKPYQCGQCGKSFR
                     QSSNLHQHHRLHHGD"
     exon            1193..1415
                     /gene="ZNF174"
                     /gene_synonym="ZSCAN8"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..2459
                     /gene="ZNF174"
                     /gene_synonym="ZSCAN8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2435..2440
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF174"
                     /gene_synonym="ZSCAN8"
                     /note="hexamer: AATAAA"
     polyA_site      2459
                     /gene="ZNF174"
                     /gene_synonym="ZSCAN8"
                     /note="major polyA site"
ORIGIN      
        1 agcccgtgct cgccggtgtc gggtcctaag tccctcggtc ttgggttccc ggagaggtga
       61 gtcggctgca ggtgggtgcg gggcgccggg ctgttcgggg taccggtgtc gcagtcgcca
      121 gtccgagctc acaccgggcg tggagttcag ctgcgcatcg ggttgcggca gcttcgccag
      181 gcctggggct ggctgggccg cggggccgcc ctggaggccg gagccactgg gcctgcggcg
      241 cctcggcagc gagcagccgc tttgctcgcg tgcaggaggc tgttcgctac ctcacacccc
      301 cggctggcgc tgtggcctcg cttagctcta ccgtttagca cccggcgaca tgcacccggt
      361 cggtttccgc caggatgcgg gagagttggg gcaagctacc tgcgacagct tgaacttttc
      421 ctagggattc cgctccaccc gccggttaga gcgtattgct cattaaatcc gagacctgtg
      481 tgcttgctac tgaaataaaa gaagtatttt ttccccagag tccttttagg acgttatgac
      541 ttttctcctt tgcaagactg caaaaaactg acaagaagac aaaactcttc ccactcccag
      601 ggaccgcgtt gtcttgagtt tggctagtaa aggctagcaa gtgaggcttt gtctctgcat
      661 cccgttcccc ctaacatcct cagagaacct tcgtttctag aatctttcta gtattcagag
      721 acttctccag ggtcatgatc ccaaaggctt aacccgttta caaggagaga gttgtctcct
      781 gacgcccaaa atggcagcta aaatggagat aactttaagc tccaacactg aagcttcctc
      841 caagcaagag agacacataa tagccaaact agaagagaaa cggggccctc ctctgcaaaa
      901 aaactgccca gatcctgagc tctgccgcca gagcttcaga cgcttttgtt atcaagaggt
      961 gtctggaccc caagaggctc tctcccagct ccgacagctc tgccgtcagt ggttgcaacc
     1021 cgagctgcac accaaggagc agattttgga gcttctggtg atggagcagt tcctgaccat
     1081 cctgcccccg gagatccagg ctcgggtcag gcatcgatgt ccaatgagca gcaaggagat
     1141 tgtgaccctc gtggaagatt ttcacagagc atccaagaaa ccaaagcagt gggtggccgt
     1201 ttgtatgcag gggcaaaagg tgctcttgga gaaaactgga tctcagcttg gagaacagga
     1261 actgccagac tttcaaccgc agactcctag gagagatctc agggagagct ctccagcaga
     1321 gccttcccag gcaggagctt atgaccggct gagcccccat cattgggaga aatccccact
     1381 cctccaagaa ccaaccccca aattggctgg gacagaggcc cccagaatga gaagtgacaa
     1441 caaggaaaat ccacaacagg aaggggctaa aggagcaaag ccatgtgcag tgtcagctgg
     1501 cagatccaaa gggaatggtc tgcagaatcc tgaaccaaga ggggcaaata tgagtgaacc
     1561 tcggttgtca cggaggcagg tcagctcccc aaatgctcaa aagccatttg ctcactacca
     1621 gagacattgc agggtggaat acatcagcag ccccctaaaa agccacccac tgagagagct
     1681 aaagaaaagc aaaggaggta aacggagtct gagcaaccgt ttgcaacatc ttggtcacca
     1741 gcccacccgc tcagcaaaga aaccctacaa atgtgatgac tgtgggaaaa gcttcacgtg
     1801 gaattcagag ctgaagagac acaagagagt ccacacagga gagagaccct acacgtgcgg
     1861 agagtgtgga aactgctttg ggcggcagtc aaccctgaag ctgcaccaga ggatccacac
     1921 tggagagaag ccataccagt gtggccagtg tgggaaaagc tttcgccaga gctcaaacct
     1981 tcaccagcat caccgacttc accatgggga ctaaaaggag cactccatgc tttagattca
     2041 cacggaaggt gtttgtgttt ctcctccccc ttacttgcat gtaaatcaca aaaactgtgt
     2101 gacttacaag gaaagcacga ggcccttgag gaatgatgat gcacattctg ctgtgaggag
     2161 gcccagaaaa ggccaaccag ggcccaaccg tgcatgatga cagggtgaag agaaggcagg
     2221 tctgggcact ggggcaaaga gaacttaagt ctctgcagag agcaaggagt aactactgag
     2281 agagaatcag gacaatcctg caggtggccc gcttactgtt aaatcgtccc tctgttgctt
     2341 tatcctctaa aatatgttaa gggataaatt ctatatatat agttatgcat ttgctgtcat
     2401 accagacaat tttttatcat gagcacactt ctttaataaa gatgagtgat ctaccagaa
//
