LOCUS       NM_021964               9514 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens zinc finger protein 148 (ZNF148), transcript variant
            2, mRNA.
ACCESSION   NM_021964
VERSION     NM_021964.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Yao,X., Xu,K., Tao,N., Cheng,S., Chen,H., Zhang,D., Yang,M.,
            Tan,M., Yu,H., Chen,P., Zhan,Z., He,S., Li,R., Wang,C., Wu,D. and
            Ren,J.
  TITLE     ZNF148 inhibits HBV replication by downregulating RXRalpha
            transcription
  JOURNAL   Virol J 21 (1), 35 (2024)
   PUBMED   38297280
  REMARK    GeneRIF: ZNF148 inhibits HBV replication by downregulating RXRalpha
            transcription.
            Erratum:[Virol J. 2024 Jun 25;21(1):145. doi:
            10.1186/s12985-024-02420-z.. PMID: 38918845]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   Szakszon,K., Lourenco,C.M., Callewaert,B.L., Genevieve,D.,
            Rouxel,F., Morin,D., Denomme-Pichon,A.S., Vitobello,A.,
            Patterson,W.G., Louie,R., Pinto E Vairo,F., Klee,E., Kaiwar,C.,
            Gavrilova,R.H., Agre,K.E., Jacquemont,S., Khadije,J., Giltay,J.,
            van Gassen,K., Mero,G., Gerkes,E., Van Bon,B.W., Rinne,T.,
            Pfundt,R., Brunner,H.G., Caluseriu,O., Grasshoff,U., Kehrer,M.,
            Haack,T.B., Khelifa,M.M., Bergmann,A.K., Cueto-Gonzalez,A.M.,
            Martorell,A.C., Ramachandrappa,S., Sawyer,L.B., Fasel,P., Braun,D.,
            Isis,A., Superti-Furga,A., McNiven,V., Chitayat,D., Ahmed,S.A.,
            Brennenstuhl,H., Schwaibolf,E.M., Battisti,G., Parmentier,B. and
            Stevens,S.J.C.
  TITLE     Further delineation of the rare GDACCF (global developmental delay,
            absent or hypoplastic corpus callosum, dysmorphic facies syndrome):
            genotype and phenotype of 22 patients with ZNF148 mutations
  JOURNAL   J Med Genet 61 (2), 132-141 (2024)
   PUBMED   37580113
  REMARK    GeneRIF: Further delineation of the rare GDACCF (global
            developmental delay, absent or hypoplastic corpus callosum,
            dysmorphic facies syndrome): genotype and phenotype of 22 patients
            with ZNF148 mutations.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9514)
  AUTHORS   Cheng,S., Liu,L., Wang,D., Li,Y., Li,S., Yuan,J., Huang,S., Xu,Z.,
            Jia,B., Li,Z. and Dong,J.
  TITLE     Upregulation of the ZNF148/PTX3 axis promotes malignant
            transformation of dendritic cells in glioma stem-like cells
            microenvironment
  JOURNAL   CNS Neurosci Ther 29 (9), 2690-2704 (2023)
   PUBMED   37063077
  REMARK    GeneRIF: Upregulation of the ZNF148/PTX3 axis promotes malignant
            transformation of dendritic cells in glioma stem-like cells
            microenvironment.
REFERENCE   4  (bases 1 to 9514)
  AUTHORS   de Klerk,E., Xiao,Y., Emfinger,C.H., Keller,M.P., Berrios,D.I.,
            Loconte,V., Ekman,A.A., White,K.L., Cardone,R.L., Kibbey,R.G.,
            Attie,A.D. and Hebrok,M.
  TITLE     Loss of ZNF148 enhances insulin secretion in human pancreatic beta
            cells
  JOURNAL   JCI Insight 8 (11), e157572 (2023)
   PUBMED   37288664
  REMARK    GeneRIF: Loss of ZNF148 enhances insulin secretion in human
            pancreatic beta cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9514)
  AUTHORS   Kuang,L.W., Zhang,C.C., Li,B.H., Liu,H.Z., Wang,H. and Li,G.C.
  TITLE     Identification of the MALAT1/miR-106a-5p/ZNF148 feedback loop in
            regulating HaCaT cell proliferation, migration and apoptosis
  JOURNAL   Regen Med 18 (3), 239-258 (2023)
   PUBMED   36710662
  REMARK    GeneRIF: Identification of the MALAT1/miR-106a-5p/ZNF148 feedback
            loop in regulating HaCaT cell proliferation, migration and
            apoptosis.
REFERENCE   6  (bases 1 to 9514)
  AUTHORS   Law,D.J., Tarle,S.A. and Merchant,J.L.
  TITLE     The human ZBP-89 homolog, located at chromosome 3q21, represses
            gastrin gene expression
  JOURNAL   Mamm Genome 9 (2), 165-167 (1998)
   PUBMED   9457682
REFERENCE   7  (bases 1 to 9514)
  AUTHORS   Taniuchi,T., Mortensen,E.R., Ferguson,A., Greenson,J. and
            Merchant,J.L.
  TITLE     Overexpression of ZBP-89, a zinc finger DNA binding protein, in
            gastric cancer
  JOURNAL   Biochem Biophys Res Commun 233 (1), 154-160 (1997)
   PUBMED   9144414
REFERENCE   8  (bases 1 to 9514)
  AUTHORS   Merchant,J.L., Iyer,G.R., Taylor,B.R., Kitchen,J.R.,
            Mortensen,E.R., Wang,Z., Flintoft,R.J., Michel,J.B. and
            Bassel-Duby,R.
  TITLE     ZBP-89, a Kruppel-like zinc finger protein, inhibits epidermal
            growth factor induction of the gastrin promoter
  JOURNAL   Mol Cell Biol 16 (12), 6644-6653 (1996)
   PUBMED   8943318
REFERENCE   9  (bases 1 to 9514)
  AUTHORS   Tommerup,N. and Vissing,H.
  TITLE     Isolation and fine mapping of 16 novel human zinc finger-encoding
            cDNAs identify putative candidate genes for developmental and
            malignant disorders
  JOURNAL   Genomics 27 (2), 259-264 (1995)
   PUBMED   7557990
REFERENCE   10 (bases 1 to 9514)
  AUTHORS   Wang,Y., Kobori,J.A. and Hood,L.
  TITLE     The ht beta gene encodes a novel CACCC box-binding protein that
            regulates T-cell receptor gene expression
  JOURNAL   Mol Cell Biol 13 (9), 5691-5701 (1993)
   PUBMED   8355710
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC108688.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 24, 2020 this sequence version replaced NM_021964.2.
            
            Summary: The protein encoded by this gene is a member of the
            Kruppel family of zinc finger DNA binding proteins. The encoded
            protein activates transcription of the T-cell receptor and
            intestinal alkaline phosphatase genes but represses transcription
            of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and
            enolase genes. Increased expression of this gene results in
            decreased patient survival rates from colorectal cancer, while
            mutations in this gene have been associated with global
            developmental delay, hypoplastic corpus callosum, and dysmorphic
            facies. [provided by RefSeq, Feb 2017].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1-11 all encode the same isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.122633.1,
                                           SRR14038197.1808080.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360647.9/ ENSP00000353863.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-224               AC108688.5         174268-174491       c
            225-305             AC108688.5         130324-130404       c
            306-441             AC108688.5         122475-122610       c
            442-790             AC108688.5         112474-112822       c
            791-916             AC108688.5         87269-87394         c
            917-1040            AC108688.5         78290-78413         c
            1041-1124           AC108688.5         76892-76975         c
            1125-1243           AC108688.5         33377-33495         c
            1244-9514           AC108688.5         24835-33105         c
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.2"
     gene            1..9514
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /note="zinc finger protein 148"
                     /db_xref="GeneID:7707"
                     /db_xref="HGNC:HGNC:12933"
                     /db_xref="MIM:601897"
     exon            1..224
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /inference="alignment:Splign:2.1.0"
     exon            225..305
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /inference="alignment:Splign:2.1.0"
     exon            306..441
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /inference="alignment:Splign:2.1.0"
     exon            442..790
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /inference="alignment:Splign:2.1.0"
     CDS             458..2842
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /note="isoform a is encoded by transcript variant 2;
                     CLL-associated antigen KW-10; CACCC box-binding protein;
                     transcription factor ZBP-89; zinc finger DNA-binding
                     protein 89"
                     /codon_start=1
                     /product="zinc finger protein 148 isoform a"
                     /protein_id="NP_068799.2"
                     /db_xref="CCDS:CCDS3031.1"
                     /db_xref="GeneID:7707"
                     /db_xref="HGNC:HGNC:12933"
                     /db_xref="MIM:601897"
                     /translation="MNIDDKLEGLFLKCGGIDEMQSSRTMVVMGGVSGQSTVSGELQD
                     SVLQDRSMPHQEILAADEVLQESEMRQQDMISHDELMVHEETVKNDEEQMETHERLPQ
                     GLQYALNVPISVKQEITFTDVSEQLMRDKKQIREPVDLQKKKKRKQRSPAKILTINED
                     GSLGLKTPKSHVCEHCNAAFRTNYHLQRHVFIHTGEKPFQCSQCDMRFIQKYLLQRHE
                     KIHTGEKPFRCDECGMRFIQKYHMERHKRTHSGEKPYQCEYCLQYFSRTDRVLKHKRM
                     CHENHDKKLNRCAIKGGLLTSEEDSGFSTSPKDNSLPKKKRQKTEKKSSGMDKESALD
                     KSDLKKDKNDYLPLYSSSTKVKDEYMVAEYAVEMPHSSVGGSHLEDASGEIHPPKLVL
                     KKINSKRSLKQPLEQNQTISPLSTYEESKVSKYAFELVDKQALLDSEGNADIDQVDNL
                     QEGPSKPVHSSTNYDDAMQFLKKKRYLQAASNNSREYALNVGTIASQPSVTQAAVASV
                     IDESTTASILESQALNVEIKSNHDKNVIPDEVLQTLLDHYSHKANGQHEISFSVADTE
                     VTSSISINSSEVPEVTPSENVGSSSQASSSDKANMLQEYSKFLQQALDRTSQNDAYLN
                     SPSLNFVTDNQTLPNQPAFSSIDKQVYATMPINSFRSGMNSPLRTTPDKSHFGLIVGD
                     SQHSFPFSGDETNHASATSTQDFLDQVTSQKKAEAQPVHQAYQMSSFEQPFRAPYHGS
                     RAGIATQFSTANGQVNLRGPGTSAEFSEFPLVNVNDNRAGMTSSPDATTGQTFG"
     exon            791..916
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1040
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1124
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1243
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..9514
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3878..3883
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /note="hexamer: AATAAA"
     polyA_site      3914
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /note="major polyA site"
     regulatory      4146..4151
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /note="hexamer: AATAAA"
     polyA_site      4169
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /note="major polyA site"
     regulatory      7714..7719
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /note="hexamer: ATTAAA"
     polyA_site      7734
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
     regulatory      7817..7822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /note="hexamer: AATAAA"
     polyA_site      7843
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
     regulatory      9491..9496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
                     /note="hexamer: AATAAA"
     polyA_site      9514
                     /gene="ZNF148"
                     /gene_synonym="BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52;
                     ZBP-89; ZFP148"
ORIGIN      
        1 atgtctgcgt ctctactgct ctgggaggag gaagagaagg aggaagagga ggaggaggag
       61 gagggggagg aagaggagaa aggcgcaggg gtgggagctg ttgccgaagc tgccacagca
      121 aaagttctcc cccctccccc cttcccctcc tctcaaggcc cctagaaagg ttggagctgc
      181 cgccgcctgc agtcggtgac cgcgcgactc ggcgcccgcc cgcggataga gggaggaatc
      241 agcagcttgg aaattcaagc acgtgatctg gcgggatggg cgtttgccta acgtatttaa
      301 tggaggaatc ggatggcata agtgattaag gtggtattga ggatttctga agcctatgaa
      361 aggtagaaac tcaaccatga tttctttttc aactctacag cattcctttc cttgaagtct
      421 tcgtttttac cttagtctcg ggcagttata cttaagcatg aacattgacg acaaactgga
      481 aggattgttt cttaaatgtg gcggcataga cgaaatgcag tcttccagga caatggttgt
      541 aatgggtgga gtgtctggcc agtctactgt gtctggagag ctacaggatt cagtacttca
      601 agatcgaagt atgcctcacc aggagatcct tgctgcagat gaagtgttac aagaaagtga
      661 aatgagacaa caggatatga tatcacatga tgaactcatg gtccatgagg agacagtgaa
      721 aaatgatgaa gagcagatgg aaacacatga aagacttcct caaggactac agtatgcact
      781 taatgtccct ataagcgtaa agcaggaaat tacttttact gatgtatctg agcaactgat
      841 gagagacaaa aaacaaatca gagagccagt agacttacag aaaaagaaga agcggaaaca
      901 acgttctccc gcaaaaatcc ttacaataaa tgaggatgga tcacttggtt tgaaaacccc
      961 taaatctcac gtttgtgagc actgcaatgc tgcctttaga acgaactatc acttacagag
     1021 acatgtcttc attcatacag gtgaaaaacc atttcaatgt agtcaatgtg acatgcgttt
     1081 catacagaag tacctgcttc agagacatga gaagattcat actggtgaaa aaccatttcg
     1141 ctgtgatgaa tgtggtatga gattcataca aaaatatcat atggaaaggc ataagagaac
     1201 tcatagtgga gaaaaacctt accagtgtga atactgttta cagtattttt ccagaacaga
     1261 tcgtgtattg aaacataaac gtatgtgcca tgaaaatcat gacaaaaaac taaatagatg
     1321 tgccatcaaa ggtggccttc tgacatctga ggaagattct ggcttttcta catcaccaaa
     1381 agacaactca ctgccaaaaa agaaaaggca gaaaacggag aaaaaatcat ctggaatgga
     1441 caaagagagt gctttggaca aatctgacct gaaaaaagac aaaaatgatt acttgcctct
     1501 ttattcttca agtactaaag taaaagatga gtatatggtt gcagaatatg ctgttgaaat
     1561 gccacattcg tcagttgggg gctcgcattt agaagatgcg tcaggagaaa tacacccacc
     1621 taagttagtt ctcaaaaaaa ttaatagtaa gagaagtctg aaacagccac tggagcaaaa
     1681 tcaaacaatt tcacctttat ccacatatga agagagcaaa gtttcaaagt atgcttttga
     1741 acttgtggat aaacaggctt tactggactc agaaggcaat gctgacattg atcaggttga
     1801 taatttgcag gaggggccca gtaaacctgt gcatagtagt actaattatg atgatgccat
     1861 gcagtttttg aagaagaagc ggtatcttca agcagcaagt aacaacagca gggaatatgc
     1921 gctgaatgtg ggtaccatag cttctcagcc ttctgtaaca caagcagctg tggcaagtgt
     1981 cattgatgaa agtaccacgg catccatatt agagtcacag gcactgaatg tggagattaa
     2041 gagtaatcat gacaaaaatg ttattccaga tgaggtactg cagactctgt tggatcatta
     2101 ttcccacaaa gctaatggac agcatgagat atccttcagt gttgcagata ctgaagtgac
     2161 ttctagcata tcaataaatt cttcagaagt accagaggtc accccgtcag agaatgttgg
     2221 atcaagctcc caagcatcct catcagataa agccaacatg ttgcaggaat actccaagtt
     2281 tctgcagcag gctttggaca gaactagcca aaatgatgcc tatttgaata gcccgagcct
     2341 taactttgtg actgataacc agaccctccc aaatcagcca gcattctctt ccatagacaa
     2401 gcaggtctat gccaccatgc ccatcaatag ctttcgatca ggaatgaatt ctccactaag
     2461 aacaactcca gataagtccc actttggact aatagttggt gattcacagc actcatttcc
     2521 cttttcaggt gatgagacaa accatgcttc tgccacatca acacaggact ttctggatca
     2581 agtgacttct cagaagaaag ctgaggccca gcctgtccac caagcttacc aaatgagctc
     2641 ctttgaacag cccttccgtg ctccctatca tggatcaaga gctggaatag ctactcaatt
     2701 tagcactgcc aatggacagg tgaaccttcg gggaccaggg acaagtgctg aattttcaga
     2761 atttcccttg gtgaatgtaa atgataatag agctgggatg acatcttcac ctgatgccac
     2821 aactggccag acttttggct aaaaaaaaaa aaaaagtgta aataatactg gcactttaga
     2881 acagattaat caagagtggg gttactctgt gtaaatggag tgctgtacag atttaagagc
     2941 aatgcgtaat aacaagttaa gctgatatga atagcaagat aatccaataa ctgcatttcg
     3001 tttggttagt cagcattctt tgaactgcct tacatgttgt cacctttata gaagcaatgc
     3061 attacttgtt ttagatcaga aacttgctat tccacccaca ccaagttaaa aaggaaaaaa
     3121 aaaagacttt cgcacaattg tttcctaact gataacattg tacattctta ggagattagt
     3181 aattgtgtga aatttactca tactgtttct aagtttttca gcatagtcat tgcacttcag
     3241 cagggaatct gagtatactt tacagacaga gtgaacttaa aagtttaatg tcaagagatt
     3301 atggcttaaa taaattagtg tgtcctatag ggggaaaaaa accaagaaac caccttttaa
     3361 aaagaatgat atgccatata cccttgattt tcattttgca ttatattgac gtgttttttt
     3421 gaaggaaaaa aagtaataaa aatctgatag tctaagactc cactatttaa aagcctaatt
     3481 actttaaaaa tatgcatact ttcagaactt ttaccaaaac acacaactgt tgaagcagtc
     3541 acttctctat ggaagtatgc atattggtgt cagtttcttt gtacagttgt acttagatat
     3601 tttttatgat ttttcatgtg caggtatcaa ggttttgaag ttttagtaaa agaaattctg
     3661 tagattacat tcccaagaac ataatgctta cacaaaatgt atattccacg ttttaaagct
     3721 taattgtatt ttactttaca tatacacttc agttaacata gagcacttag aatctatttg
     3781 gtatttttga tttctcaaag taaaaaaaaa attagatttt taggtttgat atggttgtgt
     3841 cataatcatc tcgtaattga caattttaac ttttggcaat aaaaggaaat tgggatatct
     3901 ttggaactgt aaaacctggt ttaatctttt tctttctaga ctcttgatag attggataat
     3961 taaacagtat ccagagaaac taaagaaatg ggcattttaa ttgcatattt tatcttgagt
     4021 tactttttaa tgaacactgc tcattacaac tttacaaacc aaaagtgttt actaattcca
     4081 gtaactacca tttctttttc agctagatga gtgatcggaa aatttttgtt gcatttcaaa
     4141 atctaaataa actacagact ttatccttat accatgaatg ttttttttta atactctgtc
     4201 ttaaaataat acagcatggt acaataaata gtgcttttat gtatatatac acacacacac
     4261 ttttctgaag aatgatggtt tgagttatgc gttgggcagt tttgattttt ggaagttata
     4321 ttagttattg actctttgtt acaacttttt catttttaca ttttaaattt tctgccatcg
     4381 ttgtcacaat gcacatcctg atatagcacc agtgaaaatc taaaattaat acccttggaa
     4441 aatgaaaata ttcttaattt ccattttgac tcttatactg gccctatagc tctgcagtct
     4501 ttgtttcaat atagaatatg ttatccattt aaattatttt ttcttttatt taatgttatc
     4561 ccaagactgt tctcataaag aaagggaaga aataactatc cacccttaac atccttcatt
     4621 tattttgaat atattggtgt ttttatgata aggaatataa attatattta atgtggtttc
     4681 ctgtatactt tggttattaa gttttgcttg aaatagtagt tttcccgttt gacagctttc
     4741 tttgctgcca aagttttcaa gaatttaaga cttctttgaa gtaaatattt aagttctgcc
     4801 attattgact cttaagattg tgtgtgttgt gttgtgcatt acataattac aaaaaggctt
     4861 cattcaggtg atacgttaaa aatggaactg tgctcaccct aaataggcat tggtattttt
     4921 ctcttttggt gagagtaggc atttatttct tgagttgttt tggagcctga tcaaaaattt
     4981 tgttcatgga gaacttgatg caattttgat acagtggaga ggtttttttc ggttgtttta
     5041 acatcaccag catagttttt agaatgtgac tcttgctgag catttagggt gagcttggga
     5101 aggaaggcct ttgaaaatgg tagttatgca agcagacttt caggtgttgc attcctgttt
     5161 tcaacacatt tctttaaatc tacataatgg cagacttttc taccaggtta taagcagttt
     5221 ttagataagt gatactcagc cagcataact tattgactta ccatttacgt atagtcatca
     5281 ctctcttact gtgaaattcc aaatgcctac cagagttacc ttgttctatc ataatatgac
     5341 aaacatctca acagttttgg atttccccac tttggttcag aaggttattt aatttctatc
     5401 tgggcattaa tggagaaaat aagtagcctt gtgtgctgct tcagattgaa acatggagga
     5461 tatgagatat tcttctgcaa ttcatgtttc tcatttctca aagtgagcac attgttctat
     5521 aaaaacatgc tggtacaccc ttaaactttt gatcaatctg agtgaggtgt gcttttcctc
     5581 ttgggaccta ctcacgtttg aagattggaa acatcacgtt aggcgaggca gtatctcttg
     5641 aacatcttct aaagggtttt ttaaaacctt attctcacat atttcatttg tcttgaatat
     5701 ttaagtggct cttaaacgtt ttgggtctac atgcaaatgt ggtacttaac aaggtaggaa
     5761 tccatcttct tagctctggc tgagggtgcc agccatcggt caggtcattt ttatctcaag
     5821 aggcagaagg cagttatgtc aagaattgtg ctcagggcag gatttcgttt ccacagagga
     5881 gagacacttg cagagtgcca gctaggttag atcttttgcc gcttctttca tttgattaat
     5941 ttgggttttt aaagggctgt ttaaaaaaaa aaaaaaaagg ccgggaaact ttaaaagtag
     6001 gcattactgt agtactgcat ttcttagaac attttaaact agaactcatt tttttttcac
     6061 agtatattta cttgaagaag cactataata atcattgaga agtattttga gtctgaaatt
     6121 taatttaatt ttccgtttca aattgcttta tttcagggaa ataattttcc agttgttttg
     6181 ctatattctg caaataaaaa ccgtgtttcc ttttttcact taaactttgg taggaaacaa
     6241 actaaagcag acaaacattt cttgttatgt ttgttgcttt ctttaatcca atggataaaa
     6301 aagtaaaacc ctgtaaacat tattttattt ttttatgcaa taccatgctg taaatatggt
     6361 tcatcaaata aggatgtacc tatgattgaa tctttaattc tgcacagtta gagtttatat
     6421 ataaacgtgt cttgacaatc aaggactttt atgtgagtct tcctttatga tgtttattaa
     6481 tgttatgcat tccatttgtt ttgaagtgag taccaatgtg ctaatttgta ttgtctgaaa
     6541 gtatgtaatg tttttacagc ttgtttttaa gaatctgtaa atatgtacaa acaaatcaca
     6601 gtactgcttt atgttagaag gcatatgatg ttgtactgta tgtaagcaat aatacatagc
     6661 agtgctaact ttacaagtag catcaggaaa gttctaaaaa catttcagag ttattaatta
     6721 tccttatttc atttaataat gattatcttt aatcagtttt tataagcaaa ttccattgtt
     6781 cctcctattg gaacgtaaca ctgtttacac agcataattg aagttgcagg ggagacaggc
     6841 taaatctgac ttcatctgta ctcactttca ctaagcattg aatggcctta ccactcttct
     6901 gcaagatgga ggaagaccgc aaaacttagt gcccatcaca ctgaaggaag ggatgtggtt
     6961 ttttttcctg cttctgtact gctacctaac tttgtgattt gctttggatt ttaatatttg
     7021 tttctgtttt agattcaagc ccgtaagttt tgaggtgact tcagctccat tgtgaaatag
     7081 atagttctct tcatatttca taactacatt tcaataattc taaactttct acattgattt
     7141 ttagatactt atttttcaag cttggtttct cagctgatca gatgaattta ttcaacttca
     7201 atacaaagaa agacaaacct attatagttt caagtgagta gatattatac tgtacaggac
     7261 agctatttga acacacacat cactgcttta gaaataaaac cgccaattta taaatgtata
     7321 tgacctacat tttataggaa aaaatgtttt taaatgctag tcatttatat aatgtgcttt
     7381 gaaggatttg ctagtccact tctgtcactt tttagtacac tggtatcttt tatatgtaat
     7441 gtatgctttt tattattgta gcaaagcatt tcagtagaaa gaattttgca acaatatggg
     7501 ggaaattttt cattgttgga tttgaattat actggatttt atctgtgtag tcttacttgt
     7561 ctttatttta atgctgtctg aagggaactt ggtatccaaa taaagcaggt aacctcattt
     7621 tacttcaagg gcatactgtg tagtgctgaa tttaatctga aaatctgatg attttgaaaa
     7681 gaaaaacaag tttataaaca ttgtacagaa gaaattaaat gtgtgtgatg gaaatcctga
     7741 tggtggagca cgttgaattt tctgatatat agtgttattt tcctgggatc ccctatgcct
     7801 tctttgtatt tcaactaata aaggaaaaac cctgtcatta aaactggtaa gataatagga
     7861 tattctgatt atacagtatt actattccta tcccattttc tgaccttttc tcaatcactg
     7921 taaattttta ttttctattt cctattgata attaaatatg tacataatat agacactgtt
     7981 gtatagaact aaatgaattg ggttgctatg cttgagtggg tagtgaaatg gaaatatttg
     8041 atgaatactg agagttccta aaatgctaga gcaaatttcc gagggaaagg ggggcatcgt
     8101 gtgtcttggg aaggttgcag caggttgttt cttcaactcc ctaagaaatc attgggaagg
     8161 actcaactgc aaactcggga tcagatacaa aatgggtctt tccttcccct ctcccccaaa
     8221 aaataggagc actaaatttt aaaatctact caggtgacag ttgctttgca atgaaactta
     8281 tcacattgaa attttcagtg ttaaataaaa agagatttgt gatattttta aatataaact
     8341 tttacatcag tagtcagcag cctgacaatt gaaatttggt aagtcgatat attttaaaat
     8401 attttgctct ccaaatagaa ttgtttttaa acaattggga ggttttttgt ttctttaaat
     8461 atgttttaat tgtcattgta aaaacaaaat cttgcttgac cctatattat gccatgaatg
     8521 aattgctgag cctttataat acagtgacag cttgtttcat gcatagtcat ggaacataga
     8581 gatgttcttt aaactgacct attgataaga ggtttaatga gtttcacggc tttcaacact
     8641 attgtcatat agttatcttt gccatttgga gtttataaaa ccattttatg tattgtgata
     8701 ctaaaggaag ttgttttgct ttactttaaa ttgaattatt agactaatat ttgcaaattc
     8761 tgcattttaa atgtggacac tggctgtttg aaaaataaaa tatagaatac agttttatgg
     8821 ataattttcg agctgaattt ttcacaatat tctgaaccaa ataacgaatg gtaaatatgc
     8881 aaaaatcatg gtgcatagat ataaccgtaa agagaaaaga atttctgtgt ggaattaaca
     8941 gttacacaaa ttgggtaaca attatcaggg cctttattat agctttatgt ggaatgttat
     9001 tctaaaatgc aagagtcaaa tgttactgtc attgaatatt ttagacttga aacgtgttta
     9061 tcatagagcg aagaaaatat gtgttctttt ctttacagat aagactctgt taacccactg
     9121 tcagcatacg tgggatttct tttctttttt ctttcattag tggagatttg ttttcatcca
     9181 tctaccacct tgccagtacc ctagcttgtg accagcggga tgcattgtaa gaaagttgtc
     9241 tgtggttagg agtgcagtct gggtccatgg aacaaattta aactaattgg ccctcgcaat
     9301 gatcagtcct aggaccctgg ccttattatt agcatggtgc tttaaccaat tgtacataat
     9361 aataccactg atagtctact aatgcatttc ctagatccca gtttttcttg aatgattagg
     9421 aatggtgggg agaggggagg ggagatattc tacatgatac ttctccaatc ttctaaagat
     9481 tataagaaaa aataaaaaat tgaagtcact tgaa
//
