LOCUS       NM_003439               9403 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens zinc finger with KRAB and SCAN domains 1 (ZKSCAN1),
            transcript variant 1, mRNA.
ACCESSION   NM_003439 XM_168302 XM_374496
VERSION     NM_003439.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Song,R., Ma,S., Xu,J., Ren,X., Guo,P., Liu,H., Li,P., Yin,F.,
            Liu,M., Wang,Q., Yu,L., Liu,J., Duan,B., Rahman,N.A.,
            Wolczynski,S., Li,G. and Li,X.
  TITLE     A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses
            HCC via degradation of mTOR
  JOURNAL   Mol Cancer 22 (1), 16 (2023)
   PUBMED   36691031
  REMARK    GeneRIF: A novel polypeptide encoded by the circular RNA ZKSCAN1
            suppresses HCC via degradation of mTOR.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   Li,J., Bao,S., Wang,L. and Wang,R.
  TITLE     CircZKSCAN1 Suppresses Hepatocellular Carcinoma Tumorigenesis by
            Regulating miR-873-5p/Downregulation of Deleted in Liver Cancer 1
  JOURNAL   Dig Dis Sci 66 (12), 4374-4383 (2021)
   PUBMED   33439397
  REMARK    GeneRIF: CircZKSCAN1 Suppresses Hepatocellular Carcinoma
            Tumorigenesis by Regulating miR-873-5p/Downregulation of Deleted in
            Liver Cancer 1.
REFERENCE   3  (bases 1 to 9403)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 9403)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 9403)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 9403)
  AUTHORS   Jikuya,H., Takano,J., Kikuno,R., Hirosawa,M., Nagase,T., Nomura,N.
            and Ohara,O.
  TITLE     Characterization of long cDNA clones from human adult spleen. II.
            The complete sequences of 81 cDNA clones
  JOURNAL   DNA Res 10 (1), 49-57 (2003)
   PUBMED   12693554
REFERENCE   7  (bases 1 to 9403)
  AUTHORS   Tommerup,N. and Vissing,H.
  TITLE     Isolation and fine mapping of 16 novel human zinc finger-encoding
            cDNAs identify putative candidate genes for developmental and
            malignant disorders
  JOURNAL   Genomics 27 (2), 259-264 (1995)
   PUBMED   7557990
REFERENCE   8  (bases 1 to 9403)
  AUTHORS   Rousseau-Merck,M.F., Duro,D., Berger,R. and Thiesen,H.J.
  TITLE     Chromosomal localization of two KOX zinc finger genes on chromosome
            bands 7q21-q22
  JOURNAL   Ann Genet 38 (2), 81-84 (1995)
   PUBMED   7486829
REFERENCE   9  (bases 1 to 9403)
  AUTHORS   Huebner,K., Druck,T., Croce,C.M. and Thiesen,H.J.
  TITLE     Twenty-seven nonoverlapping zinc finger cDNAs from human T cells
            map to nine different chromosomes with apparent clustering
  JOURNAL   Am J Hum Genet 48 (4), 726-740 (1991)
   PUBMED   2014798
REFERENCE   10 (bases 1 to 9403)
  AUTHORS   Thiesen,H.J.
  TITLE     Multiple genes encoding zinc finger domains are expressed in human
            T cells
  JOURNAL   New Biol 2 (4), 363-374 (1990)
   PUBMED   2288909
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY140376.1, AK074116.1,
            AC093619.5 and AA873735.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003439.3.
            
            Summary: This gene encodes a member of the Kruppel C2H2-type
            zinc-finger family of proteins. This encoded protein may function
            as a transcription factor that regulates the expression of GABA
            type-A receptors in the brain. Transcripts from this gene have been
            shown to form stable and abundant circular RNAs. Elevated
            expression of this gene has been observed in gastric cancer and the
            encoded protein may stimulate migration and invasion of human
            gastric cancer cells. [provided by RefSeq, Oct 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Both variants 1 and 6 encode isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL833578.1, SRR1803613.192182.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324306.11/ ENSP00000323148.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                HY140376.1         31-48
            19-4429             AK074116.1         1-4411
            4430-8940           AC093619.5         32704-37214         c
            8941-9403           AA873735.1         32-494              c
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..9403
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /note="zinc finger with KRAB and SCAN domains 1"
                     /db_xref="GeneID:7586"
                     /db_xref="HGNC:HGNC:13101"
                     /db_xref="MIM:601260"
     exon            1..131
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /inference="alignment:Splign:2.1.0"
     exon            132..645
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..1911
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /note="isoform a is encoded by transcript variant 1; zinc
                     finger protein 36 (KOX 18); zinc finger protein 139"
                     /codon_start=1
                     /product="zinc finger protein with KRAB and SCAN domains 1
                     isoform a"
                     /protein_id="NP_003430.1"
                     /db_xref="CCDS:CCDS34698.1"
                     /db_xref="GeneID:7586"
                     /db_xref="HGNC:HGNC:13101"
                     /db_xref="MIM:601260"
                     /translation="MMTAESREATGLSPQAAQEKDGIVIVKVEEEDEEDHMWGQDSTL
                     QDTPPPDPEIFRQRFRRFCYQNTFGPREALSRLKELCHQWLRPEINTKEQILELLVLE
                     QFLSILPKELQVWLQEYRPDSGEEAVTLLEDLELDLSGQQVPGQVHGPEMLARGMVPL
                     DPVQESSSFDLHHEATQSHFKHSSRKPRLLQSRALPAAHIPAPPHEGSPRDQAMASAL
                     FTADSQAMVKIEDMAVSLILEEWGCQNLARRNLSRDNRQENYGSAFPQGGENRNENEE
                     STSKAETSEDSASRGETTGRSQKEFGEKRDQEGKTGERQQKNPEEKTRKEKRDSGPAI
                     GKDKKTITGERGPREKGKGLGRSFSLSSNFTTPEEVPTGTKSHRCDECGKCFTRSSSL
                     IRHKIIHTGEKPYECSECGKAFSLNSNLVLHQRIHTGEKPHECNECGKAFSHSSNLIL
                     HQRIHSGEKPYECNECGKAFSQSSDLTKHQRIHTGEKPYECSECGKAFNRNSYLILHR
                     RIHTREKPYKCTKCGKAFTRSSTLTLHHRIHARERASEYSPASLDAFGAFLKSCV"
     exon            646..799
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /inference="alignment:Splign:2.1.0"
     exon            800..891
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1018
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..9403
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9379..9384
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /note="hexamer: AATAAA"
     polyA_site      9403
                     /gene="ZKSCAN1"
                     /gene_synonym="KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33"
                     /note="major polyA site"
ORIGIN      
        1 gtgactcatt gtgtctgtgt cgaggcgtcg ggagggccta agtccgtgtg cggtgccctt
       61 cggccggcct gagccccaga gtcagctccc ctttctcgcc cagcgccccc aggccgctcc
      121 cggggctcac ggaatagtaa agaaacacat cataaaacct cccaggacat aaaggtgagc
      181 acagaccctg tttggatcaa gtcagttcct ggagcctgaa tgatgactgc tgaatcacgg
      241 gaagccacgg gtctgtcccc acaggctgca caggagaagg atggtatcgt aatagtgaag
      301 gtggaagagg aagatgagga agaccacatg tgggggcagg attccaccct acaggacacg
      361 cctcctccag acccagagat attccgccaa cgcttcaggc gcttctgtta ccagaacact
      421 tttgggcccc gagaggctct cagtcggctg aaggaacttt gtcatcagtg gctgcggcca
      481 gaaataaaca ccaaggaaca gatcctggag cttctggtgc tagagcagtt tctttccatc
      541 ctgcccaagg agctccaggt ctggctgcag gaataccgcc ccgatagtgg agaggaggcc
      601 gtgacccttc tagaagactt ggagcttgat ttatcaggac aacaggtccc aggtcaagtt
      661 catggacctg agatgctcgc aagggggatg gtgcctctgg atccagttca ggagtcctcg
      721 agctttgacc ttcatcacga ggccacccag tcccacttca aacattcgtc tcggaaaccc
      781 cgcctcttac agtcacgagc tcttcctgct gcccacattc ctgcaccccc tcatgagggt
      841 agtcccagag accaggcgat ggcatctgca ctattcacag cggattccca ggcaatggtg
      901 aagatcgagg acatggctgt gtccctcatt ctggaggaat ggggatgtca gaatctggct
      961 cggaggaatc tcagtaggga caacaggcag gagaattatg ggagcgcatt tccccagggt
     1021 ggtgaaaaca ggaatgagaa cgaggagtca acctcaaagg ctgaaacctc ggaagattca
     1081 gcatcacgcg gggagacaac aggaagatcc cagaaagagt ttggagagaa acgtgaccag
     1141 gagggcaaaa caggagaaag acagcagaaa aaccctgagg agaaaaccag gaaagagaaa
     1201 agagattcag ggccagctat aggaaaggac aaaaaaacca tcacaggaga gagaggtcca
     1261 agggagaagg ggaaaggatt gggaagaagc ttcagtctga gctccaactt caccacccct
     1321 gaagaagttc ccacgggaac aaagtctcac agatgtgatg aatgtggtaa atgcttcacg
     1381 agaagttcaa gccttatccg ccataaaata atccacactg gagaaaagcc ctatgaatgt
     1441 agtgagtgtg ggaaagcctt cagtcttaac tccaaccttg tcctgcatca gaggatccac
     1501 acaggagaga aacctcatga atgtaacgag tgtggcaagg ccttcagcca cagttccaat
     1561 ctcatcctcc atcagcgcat ccactctgga gagaaacctt atgaatgtaa tgagtgcggg
     1621 aaggccttca gccagagctc ggacctcacc aagcatcaga gaattcacac gggggagaaa
     1681 ccctatgaat gtagtgaatg tggaaaagct ttcaaccgaa actcatacct gattttgcat
     1741 cggagaattc acactcgaga aaagccctac aagtgcacta agtgtggcaa ggccttcacc
     1801 cgcagctcca ccctcactct gcatcacaga atccatgcca gagagagagc ctctgagtac
     1861 agcccagcct cccttgatgc atttggcgcg ttcctgaaaa gttgtgtgta aaggaagaat
     1921 ttgccatcaa gccatttccc ccttttgttt ctaaaattat ttcagagatg tgtgctcctg
     1981 gagggaaaaa gaaatacagc ctcaacagat taaaaaacaa aagtcacact taaggatcct
     2041 tctagtcaca tcagcagtgt tctgccttta tgtagtagtt gggcatataa tccttccaca
     2101 cagcccctgc agggaaaggc taatcttacg gataatccac gtgagatttc cacacaagag
     2161 aaaagcacac gcatagtgaa atgtcagtct tttcagtaat gaggatacct ttaaggcact
     2221 cttggactct cggcaaccac aacataatag ttgaaagatc aagattggct ccacgaaagt
     2281 gatacggagg ttaggatgct acttgctgca aacaagccct actttggcca acatcctgct
     2341 tatttctcaa aaaagaggga cagtgaaaac aaaaacgaca ttgggacatg ctgctcaagg
     2401 tagttatata tacgataagt tgtatatatg atcactggta gcctaccaaa gctgtagaaa
     2461 tctaggactg tgctaatcag tatcaaacca aagatttcta tctcttcccg aaagagaggg
     2521 tatgtgcacc agtctacagt tccaaaggac tgcaacaaat gtagatggtt ctgtcctcat
     2581 ccctgagatc agttctactg aaatggcaac aacaactcca aatacatctc tcccttcttg
     2641 aaatccctaa agcactatcg cactcctaaa tgcatttctc cacaagttag cacttgattg
     2701 tatactgtct tttaatcctt cattgtttca tgtatgaagt ttttaatcac cccctaaccc
     2761 ccaaaaaaga ttcggttgtc aagggatata tttctatccc tgccagcaca gtgctggaca
     2821 tagtgagtac tgaacaccta agaggcactc attcagactg acttctacag gacttctacg
     2881 tgtgtgataa aagcctgtga atcgtgagtc cctgaaatat ggtagcctgg cccagcttct
     2941 aaagaggacc ttcgtagcca caaggcatgc taaacctcta gggtctgatg ctgtttttgt
     3001 tccaaatata aacgaaaggc actagtcagc cgcctgcata gactttctaa acagtgagta
     3061 aataccatgg aatgtcagaa atgactttat catcgtcatc ttgaagaaaa atatgaacac
     3121 tgatgaaggt catttttttt ttttgacatc tttctgtggc aaagctctta gaattctttg
     3181 cagttagctg ctgaacagta tgatgatttg gggattttct gaatcatttg tacttagata
     3241 ctggagttca gaagatgtga tttttgttgc ttttcagaaa aaggaacgtg gtagggagtg
     3301 ttttttccca cgcttttcca gtaactttgc aaaagcatta attgttcact ggcaattact
     3361 aaatgtattt tgttgctctg agaaactcag cagtgtactg agatgtggct ggctgtgcca
     3421 tcgtcatagt gcacagtgac ttttctgttt cttatcacta aagactgagg tgaggttatg
     3481 aaactttcat tggtcccatc gttgtgcgca gggtgcaact ggctacctag aagctgatgg
     3541 caggagactg tttcacacac aggagattgt gagctgtgta agtagtcatc gccactcaag
     3601 taggacaagg gtcctaccca aggctaggac cgccctgcgg aaacagaaac atagactgag
     3661 aacaaacctc aagactatca gtgtgacctc cccataacaa gagaacccca tatatagttt
     3721 gagactttcc cttgagaaac ttatactaaa actattactc atcagtcatt cactgatcag
     3781 cagctaaagt ccatgagacc aaatggtttt atatggaacc aacgaagtgg gagctaaaac
     3841 tgcacagtgg tcattctttg gcctctcctt ggctttatga cttaaaccaa ctacaacttc
     3901 cctatagctt ctaagcagtt tcatcagcat tacttgggaa aacgtgttgc aagtcaacca
     3961 gtcactagga tatttctacc catgcaacgg aagaaaaacc cattactcca gaagagtata
     4021 tctaagtaac taaaatggag gcagaaaatg ttttaaggat tttcttcaaa gaataagcca
     4081 cagcaatggt tttgtagaaa actcctgtgc ttttgagcaa ggacttttgc cctctagaaa
     4141 gcaactgagg ccaggtgcgg tggctcacgc ctgtaatccc agcactttgg gaggccgagg
     4201 tgagcggatc acctgaggtc aggagttttg agaccagcct ggccaacatg gtaaaatccc
     4261 gtctctacta aaattataaa aattagccgg gcatgatggt gggcacctgt aatcccagct
     4321 acttgggagg ctgaggcagg agaatcactt gaacccagga ggcagaggtt gcagtgagcc
     4381 aagattgcac actgcactcc agcctgggtg actagcaaaa ctccatctca aaaaaaaaaa
     4441 aaagaaagaa agaaactgaa aagtgaggtg tggaactcca ggcaacgacc caagcactta
     4501 ttttttaaga gggaaaggac tttggtcatg tttacattgg cctttggttt ttttttttct
     4561 ttcagccaca actatatgct gatataactc agccatcact tttgagtttt gtttttaaat
     4621 gaaggttaaa tgtgacctgt gccctcacat ttaattccgt ttcatggaga catcttcctg
     4681 actttgttgg atagctgcca ctagggttgc ttcgatcttc agccacatgc tgtccttgaa
     4741 gcagctattt gaagatgtgt tttctgagga aaacaggcta caactgttta ttaaaaccac
     4801 ttgcagtgca ttcgtttatc agatgctcag tgcaaagtgt aattgggtca tggtcaaaaa
     4861 cgcttgcaac atctaattgg cgttcaagat agtcattcaa aagttcttgg cccgctgaag
     4921 tctgttaaca gttgaaacat tctacagtta accattagca tgctagttgt cctaatggaa
     4981 atttttgaag agtttttcaa tatataccct acccttgcca ggaagagaag taaaatcctc
     5041 aggttgtggg gtatttgttt ctactccagc ctggcaggca aggctaaaac ctgagattat
     5101 cgatctatga gacatcttga tatgtaaagc acttaatatt aaaggcataa aagtgaaact
     5161 gctaaatatg tatagagagg ttgacgtgtg atacgtggga cagttcacat agacatcaga
     5221 gaatttattc cagaaaggag cctcctgaat gtgatgaata cggcaaagcc tttaatcaca
     5281 tctcagccct tagcatcgga aagcttatac tgtaaataaa cttgatgaat attatatgtg
     5341 aggaaaactt tcatgtatag cactcattgc ttcagacaga aaatgaattc cgtcggtatg
     5401 ttccaatcgt gatgaatttg agaaacattg caagagggag ctcaatcttg gccgggcgcc
     5461 gtggctcacg cctgtaatcg cagcactttg ggaggccgag gcaggcggat cacgaggtca
     5521 gtagttcgag accagcctgg ccaacatggt gaaaccttgt ctctactaaa aatacaaaaa
     5581 aaaattagct gggcacagtg gcgcacgcct gtagtcccag ctactcggga ggctgaggca
     5641 ggagaatggc ttgaacctgc gaggcggagg ttgcagtgag ctaagatcat gccactggca
     5701 ctccagcctt gagtgacaga gcgaggctct gtatcaaaaa aaaaagttgc gggggggtgc
     5761 tcaatcttaa ctgcagagga tctacagatg aaaaaaaatg tggggaaatg ctttaaaaaa
     5821 atagcaaaat gtgcaacttc ttacaaaaat tgttaacgtt aggtacttct atatatttta
     5881 tatgaccata atgtccgtgt gtgttttgta ccttcagtcc cttgttattg ttccgtatat
     5941 tacctgtaag cagatactgt attttatttt agcctatttg acagaacaca tcactcagaa
     6001 aaagtgaagt ttcagagcaa acagtgaaga aatcagtgtg attgtagaca aaaagtcggt
     6061 tcacagaacg gagcagcggg gagaggaagg gaaaagcttc atagtttggt gcttatcaca
     6121 tcaagagatt ggtaaatttc tgaggaaaga caggctaatg gggcactgaa atggaacaac
     6181 tcctttaaac gtgcagcctt ttgaattttt cctcaaaacc aagaagttga cctctgagct
     6241 gtcaggtgac cactgtgtgc aaaggggatg gattctcttg tcagtagacg gtcttctcca
     6301 tgaagcgaga gtaggaagtg tactggaatg gccaagtggg actgcttcag ctgaccaggt
     6361 tcttttaaac cgtagtcatg ctttcccact aactcttaaa tccttatgct tagaaaattg
     6421 aggataaggc tgggcacagt ggctcaggcc tgtaatccca gcactttggg agaccaaggc
     6481 gggtggatca caaggtcagg agatcgagac catcctggct aacatggcga aaccccgtct
     6541 ctactaaaaa tacaaaaaaa tagctgggcg tggtggcggg cgcctgtagt cccagctact
     6601 caggaggctg aggcaggagg atcacttgaa catgggaggc ggaggttgca gtgagccaag
     6661 atggcgccac tgcactccag cctgggtgat ggcgtgagac tccatgtcaa aaaaaaaaaa
     6721 aaggttgggg ggaggatagg actggccagg cacggtggct cactcctgta atctcagcac
     6781 tttgggaggc agaggtgggc ggatcacttg aggtcaggag tttgagacca gcctggccaa
     6841 cgtggtgaaa gcctgtctct actaaaaata caaaaattag ccgggcgtgg tggtgggcgc
     6901 ctgtagtccc agctactcag gaggctgagg caggagaatc acctaaatct gggaggcaga
     6961 ggttgcagtg agccgagatt gcaccactgc actccagcct gggtaacaga gactctgtct
     7021 caaaaaaaga aaaaaaaaaa agaaagaaag aaagaaaatt ggggatagga gaacagcaag
     7081 gtgggcattt cccggaattg tgtgcagatg catccagtcg tggcattgca agaagtctgt
     7141 ctgatgaagc tcgggaagca ttttgcaata ttccctttgg ctgtgttcct gtgttccctg
     7201 ctcccacttt tcttcccctg gtttgtgatt attaggagag aggttttgca aagactcgtt
     7261 gctgtgaaag aatctttttt taatttttat cctagagtca gtcactttta ttccaggtag
     7321 tcatgctgat ctgcttatcc aaagccagct aaccaggttc atcctaccat cctcatggaa
     7381 gactgtgtgt atgaattgga gtaacagaac tgaaatacac ttaaacagtg acagcagtac
     7441 ttcccagggt gggggccata tttctctgtg tcctactctg agcaacttct cagagatacg
     7501 agggggctag ggttttccca tctgggaaat ggggtgaaag tctgcagatt gttaaatgaa
     7561 atatagaatc agagaaaaag aaaagtcagt gatataaata gatcatttca tagaaattag
     7621 ggtagatttt tatttcaact actactggag aatttaataa aaggcattat ttgaaaagtt
     7681 tttctaacat agatttaggg tttttttttt tagagtggac acactacatt taaaagcaat
     7741 tattttgcta ttcagatttt ttattatctg aaaatgaaat tatctgtttt acttttcaaa
     7801 gctttgtgaa acaaacttga agttataggg aggtaagcca tctccaactc tgcaggtcaa
     7861 acgaaagttt gggaaatact tttgacatcc cacaatacag aatgtcttaa catgagaatt
     7921 gaatttcatg atgtgtggtt ccatttaata gcggacacca ccccaatctc atgttttcct
     7981 gttaccctaa aacagtggaa ggaaactggg tgtttggtag acttctaaat catggtctct
     8041 gacaatttga atctgagatt ctcacctcca tttactaaag aatcgtgact taattcaaat
     8101 tgcacagtaa tcagtaaagt gaatacgttt ttaaaatgga attttctccc ttcagcaagc
     8161 actcattaag gagtgaggct gagtatttta agatagagtg agatctgtga gtgattgaaa
     8221 ggtgatattt aaaaacttgg atttcattcc agtgtcaggt ttgggtttta agttcctttg
     8281 gtccagggaa gggtccaagc agccacagtt gccctaaatc tccatcatta agtcttccag
     8341 caaggttaag tgcagtatgg aaggagaagg gggaagagga cggtaacggc cccacactcc
     8401 aggctgagaa agagtaatta ggaggcctga ggaggggccg aggaaaggct gttggggtgt
     8461 gctggggttg gtacccgagc gccttcccct cacctcaacc agagaagagc atccggttgc
     8521 tttttaaagc ttttagcctg ccctagcaag gacaaagcat gttagattag agatgcttct
     8581 gctgatcgca ggggttctta tttgaaaaca tctatgatgg gggtggggtg ggaggagaca
     8641 ggttgtggtt atgcaggaaa atcttgtcct aaaaatatat gagtttgggg gtaaggggtg
     8701 ggatagccaa gcaaaatcag taattatttt aaaatgaaca tatgtatttt tattaacttt
     8761 tagttaaata cagattttac aacgaggtca gcataagcct aaatctatat agagggctaa
     8821 ctcaggcatt gtcttgttta tttgtagact ggattaaaaa caacctgtcc tgttttgtca
     8881 gttcccagct tcttcgttta gaataaatta gaccaaaaga agaaacgtgc ttgtctctgt
     8941 atacccgcag aatgaagtta ctgttgttaa aactggattt tttcatttta ctaggttccg
     9001 aagagtccag atgcttggta gatgttcaat acgtgatttt ttttttaatt gaatgtgttc
     9061 atttaaaatc ctccttaaca tttctagaaa gacttctttc aataaataat ggaatcttag
     9121 aggaaaagtg gttttttaaa agctagggaa ctcctccact aaaagtaacc attggaaacc
     9181 tcgaatgagg gctaaagttt taatcataag agaaaaggca gcataatgaa atgtgtacac
     9241 atacatagtc agtggtccat tttaggaagc cagtggcgtc tgataaagaa atgttaagag
     9301 tagtgaggtt gaggaaggaa attgtgggga tttgaaatat tctctttatg ttgtttctct
     9361 tctgagtcat ggtaaaacaa taaattatca tctctaggtg gca
//
