LOCUS       NM_001412797            4568 bp    mRNA    linear   PRI 13-DEC-2024
DEFINITION  Homo sapiens zinc fingers and homeoboxes 2 (ZHX2), transcript
            variant 4, mRNA.
ACCESSION   NM_001412797
VERSION     NM_001412797.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Xiao,B., Li,Y., Yang,Y., Chen,C., Gong,S., Li,H., Yao,Q. and
            Wang,L.
  TITLE     METTL3 and IGF2BP1-Mediated m6A Modification of ZHX2 Promotes Tumor
            Property of Renal Cell Carcinoma
  JOURNAL   Kidney Blood Press Res 49 (1), 787-798 (2024)
   PUBMED   39159608
  REMARK    GeneRIF: METTL3 and IGF2BP1-Mediated m6A Modification of ZHX2
            Promotes Tumor Property of Renal Cell Carcinoma.
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Creasy,K.T., Ren,H., Jiang,J., Peterson,M.L. and Spear,B.T.
  TITLE     Elongation of very long chain fatty acids-3 (Elovl3) is activated
            by ZHX2 and is a regulator of cell cycle progression
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 325 (6), G582-G592 (2023)
   PUBMED   37847682
  REMARK    GeneRIF: Elongation of very long chain fatty acids-3 (Elovl3) is
            activated by ZHX2 and is a regulator of cell cycle progression.
REFERENCE   3  (bases 1 to 4568)
  AUTHORS   Tan,S., Wang,Z., Li,N., Guo,X., Zhang,Y., Ma,H., Peng,X., Zhao,Y.,
            Li,C., Gao,L., Li,T., Liang,X. and Ma,C.
  TITLE     Transcription factor Zhx2 is a checkpoint that programs macrophage
            polarization and antitumor response
  JOURNAL   Cell Death Differ 30 (9), 2104-2119 (2023)
   PUBMED   37582865
  REMARK    GeneRIF: Transcription factor Zhx2 is a checkpoint that programs
            macrophage polarization and antitumor response.
REFERENCE   4  (bases 1 to 4568)
  AUTHORS   Ding,R., Shi,H., Guo,Y., Zeng,W. and Fan,J.
  TITLE     Zinc fingers and homeoboxes 2 inhibition could suppress the
            proliferation of ovarian cancer cells by apoptosis pathway
  JOURNAL   Clin Transl Oncol 25 (7), 2116-2126 (2023)
   PUBMED   36746874
  REMARK    GeneRIF: Zinc fingers and homeoboxes 2 inhibition could suppress
            the proliferation of ovarian cancer cells by apoptosis pathway.
REFERENCE   5  (bases 1 to 4568)
  AUTHORS   Xie,H., Zhou,J., Liu,X., Xu,Y., Hepperla,A.J., Simon,J.M., Wang,T.,
            Yao,H., Liao,C., Baldwin,A.S., Gong,K. and Zhang,Q.
  TITLE     USP13 promotes deubiquitination of ZHX2 and tumorigenesis in kidney
            cancer
  JOURNAL   Proc Natl Acad Sci U S A 119 (36), e2119854119 (2022)
   PUBMED   36037364
  REMARK    GeneRIF: USP13 promotes deubiquitination of ZHX2 and tumorigenesis
            in kidney cancer.
REFERENCE   6  (bases 1 to 4568)
  AUTHORS   Liu,G., Clement,L.C., Kanwar,Y.S., Avila-Casado,C. and Chugh,S.S.
  TITLE     ZHX proteins regulate podocyte gene expression during the
            development of nephrotic syndrome
  JOURNAL   J Biol Chem 281 (51), 39681-39692 (2006)
   PUBMED   17056598
  REMARK    GeneRIF: ZHX proteins 1, 2 and 3 are major transcriptional
            mediators of podocyte disease
            Erratum:[J Biol Chem. 2007 Apr 27;282(17):13140]
REFERENCE   7  (bases 1 to 4568)
  AUTHORS   de Andrade,T.G., Peterson,K.R., Cunha,A.F., Moreira,L.S.,
            Fattori,A., Saad,S.T. and Costa,F.F.
  TITLE     Identification of novel candidate genes for globin regulation in
            erythroid cells containing large deletions of the human beta-globin
            gene cluster
  JOURNAL   Blood Cells Mol Dis 37 (2), 82-90 (2006)
   PUBMED   16952470
  REMARK    GeneRIF: ZHX2, a transcriptional repressor, may participate in
            globin gene regulation.
REFERENCE   8  (bases 1 to 4568)
  AUTHORS   Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F.,
            Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M.
            and Zoghbi,H.Y.
  TITLE     A protein-protein interaction network for human inherited ataxias
            and disorders of Purkinje cell degeneration
  JOURNAL   Cell 125 (4), 801-814 (2006)
   PUBMED   16713569
REFERENCE   9  (bases 1 to 4568)
  AUTHORS   Kawata,H., Yamada,K., Shou,Z., Mizutani,T. and Miyamoto,K.
  TITLE     The mouse zinc-fingers and homeoboxes (ZHX) family; ZHX2 forms a
            heterodimer with ZHX3
  JOURNAL   Gene 323, 133-140 (2003)
   PUBMED   14659886
REFERENCE   10 (bases 1 to 4568)
  AUTHORS   Kawata,H., Yamada,K., Shou,Z., Mizutani,T., Yazawa,T., Yoshino,M.,
            Sekiguchi,T., Kajitani,T. and Miyamoto,K.
  TITLE     Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX
            family, functions as a transcriptional repressor
  JOURNAL   Biochem J 373 (Pt 3), 747-757 (2003)
   PUBMED   12741956
  REMARK    GeneRIF: identification as a transcriptional repressor
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016405.8 and AC104316.3.
            
            Summary: The members of the zinc fingers and homeoboxes gene family
            are nuclear homodimeric transcriptional repressors that interact
            with the A subunit of nuclear factor-Y (NF-YA) and contain two
            C2H2-type zinc fingers and five homeobox DNA-binding domains. This
            gene encodes member 2 of this gene family. In addition to forming
            homodimers, this protein heterodimerizes with member 1 of the zinc
            fingers and homeoboxes family. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.2391877.1,
                                           SRR18074969.932501.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-292               AC016405.8         114417-114708       c
            293-355             AC016405.8         32824-32886         c
            356-510             AC016405.8         22769-22923         c
            511-562             AC104316.3         73134-73185
            563-3299            AC104316.3         73535-76271
            3300-4568           AC104316.3         95485-96753
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.13"
     gene            1..4568
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="zinc fingers and homeoboxes 2"
                     /db_xref="GeneID:22882"
                     /db_xref="HGNC:HGNC:18513"
                     /db_xref="MIM:609185"
     exon            1..292
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     exon            293..355
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     exon            356..510
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     exon            511..562
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     exon            563..3299
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     CDS             782..3295
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="isoform 1 is encoded by transcript variant 4; zinc
                     fingers and homeoboxes protein 2; transcription factor
                     ZHX2; AFP regulator 1; regulator of AFP; alpha-fetoprotein
                     regulator 1; zinc finger and homeodomain protein 2"
                     /codon_start=1
                     /product="zinc fingers and homeoboxes protein 2 isoform 1"
                     /protein_id="NP_001399726.1"
                     /db_xref="GeneID:22882"
                     /db_xref="HGNC:HGNC:18513"
                     /db_xref="MIM:609185"
                     /translation="MASKRKSTTPCMVRTSQVVEQDVPEEVDRAKEKGIGTPQPDVAK
                     DSWAAELENSSKENEVIEVKSMGESQSKKLQGGYECKYCPYSTQNLNEFTEHVDMQHP
                     NVILNPLYVCAECNFTTKKYDSLSDHNSKFHPGEANFKLKLIKRNNQTVLEQSIETTN
                     HVVSITTSGPGTGDSDSGISVSKTPIMKPGKPKADAKKVPKKPEEITPENHVEGTARL
                     VTDTAEILSRLGGVELLQDTLGHVMPSVQLPPNINLVPKVPVPLNTTKYNSALDTNAT
                     MINSFNKFPYPTQAELSWLTAASKHPEEHIRIWFATQRLKHGISWSPEEVEEARKKMF
                     NGTIQSVPPTITVLPAQLAPTKVTQPILQTALPCQILGQTSLVLTQVTSGSTTVSCSP
                     ITLAVAGVTNHGQKRPLVTPQAAPEPKRPHIAQVPEPPPKVANPPLTPASDRKKTKEQ
                     IAHLKASFLQSQFPDDAEVYRLIEVTGLARSEIKKWFSDHRYRCQRGIVHITSESLAK
                     DQLAIAASRHGRTYHAYPDFAPQKFKEKTQGQVKILEDSFLKSSFPTQAELDRLRVET
                     KLSRREIDSWFSERRKLRDSMEQAVLDSMGSGKKGQDVGAPNGALSRLDQLSGAQLTS
                     SLPSPSPAIAKSQEQVHLLRSTFARTQWPTPQEYDQLAAKTGLVRTEIVRWFKENRCL
                     LKTGTVKWMEQYQHQPMADDHGYDAVARKATKPMAESPKNGGDVVPQYYKDPKKLCEE
                     DLEKLVTRVKVGSEPAKDCLPAKPSEATSDRSEGSSRDGQGSDENEESSVVDYVEVTV
                     GEEDAISDRSDSWSQAAAEGVSELAESDSDCVPAEAGQA"
     exon            3300..4568
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4540..4545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="hexamer: AATAAA"
     regulatory      4552..4557
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="hexamer: ACTAAA"
     polyA_site      4565
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
     polyA_site      4568
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="major polyA site"
ORIGIN      
        1 atgtgggttt tggcgtagat tccccactga tcgaggcatt ttttttccct ttttttttcc
       61 tttttttttt tcttttaaaa attttggcca tcgttctccg tacgggggct ttttctgtct
      121 gtctgtctgg ctggcaggct ggctttcccc ctctttccca cggagcccga gccgggcgcc
      181 cggtggggag tggggagtgg gtggggggag ccagcagagt tccattttgg aacgcccgtg
      241 ccgcgtctcc gcgttcccag cccgggtccc cgcgttcaca gccccagcgc aggtctggat
      301 gtaccgactg cttttggaat aaaaagattc ccaggatgtg agcaacacgg gaccgcccct
      361 gagctggtgc ctgtccgtac ttggcccctg tgcagcactc agtagatggt cactggttga
      421 gcagcatcag agcgggcatg cagagaaggg cccctcgtcc tgcggccggt gcaggacgcc
      481 atctttgctt caggtgatgc tcccactctg cgcctgagta aatttcactt gccaacccct
      541 ggggacacat ctcgcttccg agatatgatg cttcctggtg tgtttagtgg ttggtgccat
      601 tccaattttc tgtgctgaaa tcattctgaa aactcaaaca gtagacttca gcacacaagg
      661 aaagccaaag ccatttgagg gggaataaag ccaaaagcct ttcaccttat tcgttccaag
      721 aatctcaccg ccccctcctt atccccctcc aaaaataagc cattgcacac agacaggcag
      781 catggctagc aaacgaaaat ctacaactcc atgcatggtt cggacatcac aagtagtaga
      841 acaagatgtg cccgaggaag tagacagggc caaagagaaa ggaatcggca caccacagcc
      901 tgacgtggcc aaggacagtt gggcagcaga acttgaaaac tcttccaaag aaaacgaagt
      961 gatagaggtg aaatctatgg gggaaagcca gtccaaaaaa ctccaaggtg gttatgagtg
     1021 caaatactgc ccctactcca cgcaaaacct gaacgagttc acggagcatg tcgacatgca
     1081 gcatcccaac gtgattctca accccctcta cgtgtgtgca gaatgtaact tcacaaccaa
     1141 aaagtacgac tccctatccg accacaactc caagttccat cccggggagg ccaacttcaa
     1201 gctgaagtta attaaacgca ataatcaaac tgtcttggaa cagtccatcg aaaccaccaa
     1261 ccatgtcgtg tccatcacca ccagtggccc tggaactggt gacagtgatt ctgggatctc
     1321 ggtgagtaaa acccccatca tgaagcctgg aaaaccaaaa gcggatgcca agaaggtgcc
     1381 caagaagccc gaggagatca cccccgagaa ccacgtggaa gggaccgccc gcctggtgac
     1441 agacacagct gagatcctct cgagactcgg cggggtggag ctcctccaag acacattagg
     1501 acacgtcatg ccttctgtac agctgccacc aaatatcaac cttgtgccca aggtccctgt
     1561 cccactaaat actaccaaat acaactctgc cctggataca aatgccacga tgatcaactc
     1621 tttcaacaag tttccttacc cgacccaggc tgagttgtcc tggctgacag ctgcctccaa
     1681 acacccagag gagcacatca gaatctggtt tgccacccag cgcttaaagc atggcatcag
     1741 ctggtcccca gaagaggtgg aggaggcccg gaagaagatg ttcaacggca ccatccagtc
     1801 agtacccccg accatcactg tgctgcccgc ccagttggcc cccacaaagg tgacgcagcc
     1861 catcctccag acggctctac cgtgccagat cctcggccag actagcctgg tgctgactca
     1921 ggtgaccagc gggtcaacaa ccgtctcttg ctcccccatc acacttgccg tggcaggagt
     1981 caccaaccat ggccagaaga gacccttggt gactccccaa gctgcccccg aacccaagcg
     2041 tccacacatc gctcaggtgc cagagccccc acccaaggtg gccaaccccc cgctcacacc
     2101 agccagtgac cgcaagaaga caaaggagca gatagcacat ctcaaggcca gctttctcca
     2161 gagccagttc cctgacgatg ccgaggttta ccggctcatc gaggtgactg gccttgccag
     2221 gagcgagatc aagaagtggt tcagtgacca ccgatatcgg tgtcaaaggg gcatcgtcca
     2281 catcaccagc gaatcccttg ccaaagacca gttggccatc gcggcctccc gacacggtcg
     2341 cacgtatcat gcgtacccag actttgcccc ccagaagttc aaagagaaaa cacagggtca
     2401 ggttaaaatc ttggaagaca gctttttgaa aagttctttt cctacccaag cagaactgga
     2461 tcggctaagg gtggagacca agctgagcag gagagagatc gactcctggt tctcggagag
     2521 gcggaagctt cgagacagca tggaacaagc tgtcttggat tccatggggt ctggcaaaaa
     2581 aggccaagat gtgggagccc ccaatggtgc tctgtctcga ctcgaccagc tctccggtgc
     2641 ccagttaaca agttctctgc ccagcccttc gccagcaatt gcaaaaagtc aagaacaggt
     2701 tcatctcctg aggagcacgt ttgcaagaac ccagtggcct actccccagg agtacgacca
     2761 gttagcggcc aagactggcc tggtccgaac tgagattgtg cgttggttca aggagaacag
     2821 atgcttgctg aaaacgggaa ccgtgaagtg gatggagcag taccagcacc agcccatggc
     2881 agatgatcac ggctacgatg ccgtagcaag gaaagcaaca aaacccatgg ccgagagccc
     2941 aaagaacggg ggtgatgtgg ttccacaata ttacaaggac cccaaaaagc tctgcgaaga
     3001 ggacttggag aagttggtga ccagggtaaa agtaggcagc gagccagcaa aagactgttt
     3061 gccagcaaag ccctcagagg ccacctcaga ccggtcagag ggcagcagcc gggacggcca
     3121 gggtagcgac gagaacgagg agtcgagcgt tgtggattac gtggaggtga cggtcgggga
     3181 ggaggatgcg atctcagata gatcagatag ctggagtcag gctgcggcag aaggtgtgtc
     3241 ggaactggct gaatcagact ccgactgcgt ccctgcagag gctggccagg cctagacagg
     3301 gaagtctgtt agaactgctg tgctgatcaa cgggacgctc cgtctttgaa gaaagaagag
     3361 atggtctctc cccagccatg ggccaccctt gccagtgact ccaagtggaa ctacttagct
     3421 cgcgtgtgcc tggagggtgc gggaagtcca gcgactctca gacgcacctc ccagaggacc
     3481 ggtgggaatt gttcatagtg ccaaagtcct actactgcgt tttcaatggg tccttgtaca
     3541 tagtttgctc ctctgcccta gccctcacct cttgctatac tggaaccgat ttgtacaatg
     3601 tgggaatttt gttacctttt taatcaaggg caacttcctt ttccagcact accattgtaa
     3661 ggtttttttc aggagggagg gctaaccacc ttgcttttct cttttctctt tttctttttt
     3721 ttatttttgt tttattaatt tggggaaagg ggtgttagca ttagtgccat gatatctact
     3781 ggattttaag tagggagact ttatttttaa aggtaggttg aaatttggga gatttctcgg
     3841 caggaagggc tgaaatccag gcccctgtct caacttggag agaggtgaca gacggcagat
     3901 cttccaaatc aaattccttt ccagttcttc ccctggctgc ctttttgggg gtccctgcct
     3961 tagccccaca caaggctttc tgaactgcca agaggggatc tggcttctca actgctcggc
     4021 ctcttgggcc aggctgtgcc cagccagccc tgggagaact gggtagcagg tggctgactt
     4081 ctttaagcac ctttctaaat accagcagaa gaggctcccg cctctgttag catgatcagt
     4141 actattgtga cattaaaaca acaacaataa gatcttccta tctggagggt acagaggtga
     4201 atggctttgg ttttcatttc tcttcttcac tgccttttct cggtgtggta tttgacaaga
     4261 ttttagctca aagcctcacc atgaattgat tttttttgtt tgtgtgtgtg tttgttttgg
     4321 gacaatttta gatacctgag tgcacttttt cagttagtcc taacttttaa aagaaggaaa
     4381 accaagagac atatctggtg tacgtgttgc agtatgaact ctggttgcaa tccctccccg
     4441 tcccacactg ccccccattt gagtacaccg cacaagtcaa acgctaggaa gtttgaataa
     4501 aaccaatttt tctaacttgt tgctcatttg ttgtaactca ataaagcaaa gactaaacat
     4561 ttttataa
//
