LOCUS       NM_018392               6648 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript
            variant 1, mRNA.
ACCESSION   NM_018392
VERSION     NM_018392.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Yan,S., Song,M., Ping,J., Lai,S.T., Cao,X.Y., Bai,C.J., Xie,D.F.,
            Guan,H., Gao,S.S. and Zhou,P.K.
  TITLE     ZGRF1 promotes end resection of DNA homologous recombination via
            forming complex with BRCA1/EXO1
  JOURNAL   Cell Death Discov 7 (1), 260 (2021)
   PUBMED   34552057
  REMARK    Erratum:[Cell Death Discov. 2022 Nov 18;8(1):460. doi:
            10.1038/s41420-022-01248-2.. PMID: 36400755]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Brannvoll,A., Xue,X., Kwon,Y., Kompocholi,S., Simonsen,A.K.W.,
            Viswalingam,K.S., Gonzalez,L., Hickson,I.D., Oestergaard,V.H.,
            Mankouri,H.W., Sung,P. and Lisby,M.
  TITLE     The ZGRF1 Helicase Promotes Recombinational Repair of
            Replication-Blocking DNA Damage in Human Cells
  JOURNAL   Cell Rep 32 (1), 107849 (2020)
   PUBMED   32640219
  REMARK    GeneRIF: The ZGRF1 Helicase Promotes Recombinational Repair of
            Replication-Blocking DNA Damage in Human Cells.
REFERENCE   3  (bases 1 to 6648)
  AUTHORS   Peter,B., Wijsman,E.M., Nato,A.Q. Jr., Matsushita,M.M.,
            Chapman,K.L., Stanaway,I.B., Wolff,J., Oda,K., Gabo,V.B. and
            Raskind,W.H.
  CONSRTM   University of Washington Center for Mendelian Genomics
  TITLE     Genetic Candidate Variants in Two Multigenerational Families with
            Childhood Apraxia of Speech
  JOURNAL   PLoS One 11 (4), e0153864 (2016)
   PUBMED   27120335
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6648)
  AUTHORS   Gao,C., Wang,N., Guo,X., Ziegler,J.T., Taylor,K.D., Xiang,A.H.,
            Hai,Y., Kridel,S.J., Nadler,J.L., Kandeel,F., Raffel,L.J.,
            Chen,Y.D., Norris,J.M., Rotter,J.I., Watanabe,R.M.,
            Wagenknecht,L.E., Bowden,D.W., Speliotes,E.K., Goodarzi,M.O.,
            Langefeld,C.D. and Palmer,N.D.
  TITLE     A Comprehensive Analysis of Common and Rare Variants to Identify
            Adiposity Loci in Hispanic Americans: The IRAS Family Study
            (IRASFS)
  JOURNAL   PLoS One 10 (11), e0134649 (2015)
   PUBMED   26599207
  REMARK    GeneRIF: Two novel loci IDH1 and ZGRF1 are associated with
            adiposity. [meta-analysis]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6648)
  AUTHORS   Kwak,S.H., Park,Y.J., Go,M.J., Lee,K.E., Kim,S.J., Choi,H.S.,
            Kim,T.H., Choi,S.H., Lim,S., Kim,K.W., Park,D.J., Kim,S.S.,
            Lee,J.Y., Park,K.S., Jang,H.C. and Cho,N.H.
  TITLE     A genome-wide association study on thyroid function and
            anti-thyroid peroxidase antibodies in Koreans
  JOURNAL   Hum Mol Genet 23 (16), 4433-4442 (2014)
   PUBMED   24722205
REFERENCE   6  (bases 1 to 6648)
  AUTHORS   Kalsi,G., Kuo,P.H., Aliev,F., Alexander,J., McMichael,O.,
            Patterson,D.G., Walsh,D., Zhao,Z., Schuckit,M., Nurnberger,J. Jr.,
            Edenberg,H., Kramer,J., Hesselbrock,V., Tischfield,J.A.,
            Vladimirov,V., Prescott,C.A., Dick,D.M., Kendler,K.S. and
            Riley,B.P.
  TITLE     A systematic gene-based screen of chr4q22-q32 identifies
            association of a novel susceptibility gene, DKK2, with the
            quantitative trait of alcohol dependence symptom counts
  JOURNAL   Hum Mol Genet 19 (12), 2497-2506 (2010)
   PUBMED   20332099
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Hum Mol Genet. 2010 Oct 15;19(20):4121. Hesselbrock,
            Victor [added]; Tischfield, Jay A [added]]
REFERENCE   7  (bases 1 to 6648)
  AUTHORS   Bechtel,S., Rosenfelder,H., Duda,A., Schmidt,C.P., Ernst,U.,
            Wellenreuther,R., Mehrle,A., Schuster,C., Bahr,A., Blocker,H.,
            Heubner,D., Hoerlein,A., Michel,G., Wedler,H., Kohrer,K.,
            Ottenwalder,B., Poustka,A., Wiemann,S. and Schupp,I.
  TITLE     The full-ORF clone resource of the German cDNA Consortium
  JOURNAL   BMC Genomics 8, 399 (2007)
   PUBMED   17974005
  REMARK    Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL708767.1, BC044799.1,
            AK299831.1, BM721145.1, DA199760.1, CN297341.1, AK126054.1 and
            BF056790.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 6, 2020 this sequence version replaced NM_018392.4.
            
            Summary: The encoded protein contains GRF zinc finger (zf-GRF) and
            transmembrane domains. GRF zinc fingers are found in a number of
            DNA-binding proteins. Alternative splicing results in multiple
            transcript variants encoding different isoforms. [provided by
            RefSeq, Apr 2017].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.537554.1,
                                           SRR18074969.2155528.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000505019.6/ ENSP00000424737.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 AL708767.1         10-18
            10-1476             BC044799.1         1-1467
            1477-2843           AK299831.1         1563-2929
            2844-3011           BC044799.1         2835-3002
            3012-3101           BM721145.1         602-691
            3102-3217           BC044799.1         3093-3208
            3218-3399           DA199760.1         390-571
            3400-4022           CN297341.1         85-707
            4023-6643           AK126054.1         493-3113
            6644-6648           BF056790.1         10-14               c
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q25"
     gene            1..6648
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /note="zinc finger GRF-type containing 1"
                     /db_xref="GeneID:55345"
                     /db_xref="HGNC:HGNC:25654"
     exon            1..59
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            60..146
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..6440
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /EC_number="5.6.2.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     GRF-type zinc finger domain-containing protein 1; protein
                     ZGRF1; 5'-3' DNA helicase ZGRF1"
                     /codon_start=1
                     /product="5'-3' DNA helicase ZGRF1 isoform 1"
                     /protein_id="NP_060862.3"
                     /db_xref="CCDS:CCDS3700.2"
                     /db_xref="GeneID:55345"
                     /db_xref="HGNC:HGNC:25654"
                     /translation="MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGAC
                     LESLFLKCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSG
                     RSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPL
                     FPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVN
                     SGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTE
                     HFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQ
                     SSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVG
                     NNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIK
                     GSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKM
                     DNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVN
                     DGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMG
                     ICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKS
                     KGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQL
                     LNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDH
                     VEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGT
                     QQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKE
                     ETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYE
                     NFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKAR
                     TLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMIN
                     SNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKW
                     LKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQM
                     IKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEIS
                     GSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQ
                     KALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPK
                     ADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGF
                     DFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELD
                     TFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYF
                     NPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELI
                     QVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVI
                     LFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIA
                     KPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVR
                     VVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPP
                     TIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGV
                     TEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMI
                     GVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGF
                     IDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLN
                     HLLKDYFEKQVEEKQKKKSEKEKSKDKSHS"
     exon            147..227
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            228..287
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            288..476
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            477..2727
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            2728..2792
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            2793..2843
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            2844..2927
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            2928..3101
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            3102..3252
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            3253..3902
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            3903..4041
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            4042..4226
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            4227..4423
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            4424..4563
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            4564..4707
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            4708..4822
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            4823..5085
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            5086..5245
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            5246..5323
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            5324..5471
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            5472..5599
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            5600..5723
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            5724..5900
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            5901..6035
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            6036..6297
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     exon            6298..6648
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6532..6537
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /note="hexamer: ATTAAA"
     polyA_site      6555
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /note="major polyA site"
     regulatory      6629..6634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /note="hexamer: AATAAG"
     polyA_site      6648
                     /gene="ZGRF1"
                     /gene_synonym="C4orf21"
                     /note="major polyA site"
ORIGIN      
        1 aatgacgtca cgttgtttcg cgggagaaat tcgaaagtgg attttgtgca atttgacagg
       61 tggtataaat agttgaatat ttcctatttt ctggacccag ctggtttata acttcagaag
      121 aaataatgga aagccaagaa tttattgttc tatatactca tcaaaagatg aagaagtcaa
      181 aagtgtggca agatggaatt ctgaagatca ctcacttagg aaacaaagca attttatatg
      241 atgacaaagg agcatgtttg gagagtctgt ttcttaaatg ccttgaggtg aaacctggag
      301 atgacttaga aagtgatcga tacttaatca cagttgaaga ggttaaagtt gctggagcca
      361 taggtattgt taagcagaat gtcaataaag aagcaccaga gttaaattca aggacattta
      421 tatcctctgg ccgatctctt ggatgtcagc cctctggctt aaaaaggaag tttactggtt
      481 ttcaaggacc acgtcaggtt ccaaagaaaa tggttattat ggaaagtggt gaatcagctg
      541 catcacatga ggctaagaaa actggcccta ctattttttc tccattctgc agcatgcctc
      601 ctttgtttcc tactgttggc aagaaagatg taaataatat actggcagac cctgagaaca
      661 ttgtgactta caagaacagg gagagaaatg ccatggattt ttcttcggtt ttttctccat
      721 ccttccagat taacccagaa gtgctgtgtg aagaaaatta tttttgctca cctgtcaatt
      781 ctggaaataa gctttcagac tctttactga ccaatgagcc tgtgaaaaga gatagtttgg
      841 catctcacta ttcaggagtt tcacaaaaca tcagaagcaa agcacagata ttagctcttc
      901 tgaagtccga atcatctagt tcatgtgagg aactaaattc tgagatgaca gagcattttc
      961 ctcaaaaaca accacaagga agtttaaaaa ttgctactaa accaaagtac ctaattcaac
     1021 aggaagagtg tgctgagatg aagagcacag aaaatttata ctaccagcat caatcagaaa
     1081 ataccatgag aaataaaagc cggtgggcca tgtatttatc ctcacagagt tcacctatac
     1141 attcttctac tgtagatggg aatgatacag aaaggaaacc caaggcccag gaagatgatg
     1201 taaattctaa tttgaaagac ctttcattac aaaaaattat acagttcgtt gaaacgtatg
     1261 ctgaagagag gaaaaagtat aatgtagacc agtcagtcgg taataatgat ccatcctgga
     1321 atcaggaagt aaaattagaa attccttcat tcaatgaaag cagtagctta caggttactt
     1381 gtagcagtgc agaaaatgat ggtatattat cagaatctga cattcaagaa gataataaaa
     1441 taccttttaa tcaaaatgac aaggggtgca ttaaaggatc agttctcatt aaagaaaatg
     1501 ctcaggaggt aaatacatgt ggaacactgg aaaaggagta tgaacaatca gagtcatctc
     1561 tgccagaact gaaacatctc caaattgaat ctagtaataa ttctaggatc tctgatgaca
     1621 ttacagacat gatttctgaa agtaaaatgg ataatgaaag tcttaatagt attcatgaat
     1681 ctctgagtaa tgtaacacaa ccatttttgg aggtaacttt taatctgaac aattttgaga
     1741 ccagtgacac tgaggaggaa tcacaggaaa gcaacaaaat ttcccaggat tcagagagtt
     1801 gggtaaagga cattttggtt aatgatggca attcatgttt tcaaaagagg tctgagaata
     1861 caaactgtga agagattgag ggtgaacatt tgccattctt aacatctgtt agtgacaaac
     1921 ctacagtgac atttcctgtt aaagagactc tgccatcaca gttttgtgat aaaacttatg
     1981 tgggttttga catgggaata tgtaaaactg aaaacacagg aaaagaaata gaggagtata
     2041 gtgacacatt aagcaatttt gaatctttca agtggactga tgctgtatac ggagataata
     2101 aagaagatgc taataaacct attcaagaag tcagaattaa ctatgatttt gctttacccc
     2161 cgaataaatc taaaggtata aacatgaatt tacatattcc tcatattcaa aaccagattg
     2221 ctgaaaacag taatctattt tcagaagatg ctcaacctca gccttttatt ttgggaagtg
     2281 acttagacaa aaatgatgaa catgttttac cctcaacttc tagtagtgac aacagtgtcc
     2341 aactattaaa taccaatcag aatcactatg aatgtattgc acttgataaa tcaaataccc
     2401 acatttccaa ttctttgttt tacccactgg gaaaaaagca tcttatttcc aaagacacag
     2461 aagcacatat atctgaacct gaagatttgg gaaagattag aagtccaccc cctgaccatg
     2521 ttgaggttga aactgccaga gaaggcaaac aatactggaa tcctagaaat tcttcagaac
     2581 tttctggatt agtaaatacc atttctattt taaagtcgct atgtgaacac agtactgctt
     2641 tagacagctt ggaaatattg aaaaagaaaa atactgtctt tcagcaagga actcaacaga
     2701 cgtatgagcc agatagccct cctgaagtga ggaaaccatt tattacagta gtttcaccaa
     2761 agtctcctca tctgcacaag gattctcaac agatactaaa agaagatgaa gttgaactaa
     2821 gtgaaccact tcagtctgtg cagttctctt cctcgggaag taaagaagag actgcttttc
     2881 aagctgttat tcctaaacaa atagagagaa aaacctgtga ccctaagcct gttgagtttc
     2941 aaggacatca agtaaaagga tcagctacta gtggtgtaat ggtcagagga cacagctcac
     3001 agctaggatg cagtcagttt ccagatagca ctgagtatga aaactttatg acagagacac
     3061 cagagttacc tagcacgtgt atgcagattg acttcttgca ggtgacatca ccagaagaaa
     3121 acatctctac attgagccct gtttctacct tttctttgaa ctcaagagat gaagacttca
     3181 tggtagaatt ctctgagacg tccctgaaag caagaacttt acctgatgat cttcattttc
     3241 tcaacttgga ggggatgaaa aaatcccgtt ctctggagaa tgagaacctt caaaggcttt
     3301 cattattaag tagaacccag gttccactta ttactttgcc acgtactgat gggccacctg
     3361 acttagactc tcattcgtat atgatcaact ctaacacata cgagtcttct ggctccccca
     3421 tgctcaattt gtgtgaaaag tcagcagttc tttcgtttag cattgagcct gaggaccaaa
     3481 atgaaacctt tttctctgaa gaatctaggg aagtgaatcc aggggatgtt tcacttaata
     3541 atatatctac tcagagcaag tggctgaaat atcaaaacac atcccaatgc aacgtggcta
     3601 ctccaaacag agttgataag agaataactg atggcttctt tgctgaggct gtttctggga
     3661 tgcattttag agacacaagt gaaagacaga gtgatgctgt caatgaaagc tctttagact
     3721 ctgtgcattt gcaaatgata aaaggcatgc tctatcaaca gcggcaggat tttagcagtc
     3781 aagattcggt ttccagaaag aaagtacttt ctctgaattt aaagcagact tctaagacag
     3841 aggaaattaa aaatgtatta ggagggtcta cctgctacaa ctacagtgta aaggatttac
     3901 aggagataag tggctctgag ctgtgctttc caagtgggca gaaaataaaa tctgcttatc
     3961 ttccccaaag gcaaattcac ataccagctg tttttcagtc tcctgctcat tataagcaga
     4021 ctttcacatc ttgcctcata gaacatctaa atatattgct gtttgggtta gcacaaaacc
     4081 tgcagaaagc tctttcaaaa gttgacatat cattttatac atcattgaag ggagagaaac
     4141 tgaaaaacgc agaaaataat gtaccatcct gccatcatag tcaacctgca aaacttgtca
     4201 tggttaaaaa ggaaggtcca aataagggtc gtctctttta tacatgtgat ggacccaaag
     4261 ctgatcgatg taaattcttt aaatggcttg aggacgtgac tccaggatat tcaacacagg
     4321 aaggagctcg acctggcatg gttttaagtg atattaagag tattggctta tatttaagaa
     4381 gtcaaaagat accactttat gaggaatgcc agcttttggt gagaaaagga tttgattttc
     4441 agagaaaaca gtatggcaaa ctaaagaagt ttactactgt aaatcctgag ttttataatg
     4501 aaccaaaaac caaactttat cttaagctaa gtcggaagga aagatcttca gcttatagca
     4561 aaaatgatct ttgggtggtt tcaaaaaccc tagactttga gctggatact tttatcgcat
     4621 gtagtgcttt ctttggacca tcatctatca atgagataga aatactgcct ttgaaaggct
     4681 atttcccttc taattggccc actaacatgg ttgtccatgc gttattggtt tgtaatgcta
     4741 gcacagaact gactactttg aaaaacattc aggactactt taatccagct actctacctc
     4801 taacacagta cctgttaaca acgtcttcgc caactatagt tagtaacaaa agagtcagta
     4861 agagaaaatt tatcccacca gccttcacaa atgtcagtac aaaatttgaa ctactcagcc
     4921 taggagcaac attgaagtta gctagtgagt tgattcaggt acacaagtta aacaaggatc
     4981 aagctacagc tctaattcaa atagctcaaa tgatggcatc acatgaaagc attgaagaag
     5041 tgaaggaact gcaaactcat accttcccta tcacaatcat acatggtgtg tttggagcag
     5101 gaaagagtta cttgctggca gtggtgattt tgttctttgt acagctgttt gaaaagagtg
     5161 aagctcccac cattggaaat gcaaggccgt ggaaacttct gatttcttct tctactaatg
     5221 tggctgttga cagagtactt cttgggcttc tcagtcttgg atttgaaaac tttatcagag
     5281 ttgggagtgt taggaagatt gccaaaccaa ttttacctta tagcttgcat gctggctcag
     5341 aaaatgaaag tgaacagtta aaagaactac atgcactaat gaaagaagac ctgactccta
     5401 cggaaagagt ctatgtgaga aaaagcattg agcagcataa actggggacc aatagaaccc
     5461 tgctgaagca ggttcgagta gttggagtta cctgtgcagc ctgcccattc ccatgcatga
     5521 atgatcttaa atttcctgta gttgtgctgg atgagtgtag tcagataact gaaccggcct
     5581 ctctccttcc cattgcaagg tttgagtgtg aaaagctgat tcttgttggg gatcccaaac
     5641 agctacctcc tactattcag ggttctgatg cagctcatga aaatggattg gaacaaactc
     5701 tttttgatcg actttgctta atgggtcaca agccaattct attgagaact caataccgtt
     5761 gtcatcctgc aatcagtgct attgctaatg atctgtttta caaaggagcc ctcatgaatg
     5821 gtgtaacaga aatagagcgg agccctttat tggaatggct accaaccctg tgtttttata
     5881 atgttaaagg actagaacag atagaaagag ataacagctt tcataatgtg gcagaagcta
     5941 cgtttacact caagctgatt caatcactga ttgcaagtgg aatagcaggc tctatgattg
     6001 gtgtgataac attatacaaa tcccagatgt acaagctttg tcatttactc agtgctgtgg
     6061 actttcacca tcctgatatt aaaactgtgc aggtgtccac agtagatgct tttcagggag
     6121 ctgaaaagga gatcattatt ctgtcctgtg taaggacaag acaagtagga ttcattgatt
     6181 cagaaaaaag aatgaatgtt gcattgacta gaggaaagag gcatttgttg attgtgggaa
     6241 atttagcctg tttgaggaaa aatcaacttt ggggacgagt gatccaacac tgcgaaggaa
     6301 gggaagatgg attgcaacat gcaaaccagt atgaaccaca gctgaaccat ctccttaaag
     6361 attattttga aaaacaagtg gaagaaaaac agaagaaaaa gagtgaaaaa gagaaatcta
     6421 aagataaatc tcattcataa aaagacatgg tgtaaatatt ttgtatttat gtaaattcag
     6481 actcatttta catgatatat tttttatatt tttattactc taaaccctct tattaaaaat
     6541 atgatattta aataacatag taaacacatg taaaaatttt gttcttcaaa aaagtgtaca
     6601 aaaggtagta taaaatccta ctaataaaaa taagcttttt tctaagaa
//
