LOCUS       NM_152338               3116 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens zymogen granule protein 16 (ZG16), mRNA.
ACCESSION   NM_152338 XM_930062
VERSION     NM_152338.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Meng,H., Li,L., Nan,M., Ding,Y., Li,Y. and Zhang,M.
  TITLE     ZG16 enhances the maturation of dendritic cells via induction of
            CD40 and contributes to the antitumor immunity in pancreatic cancer
  JOURNAL   Oncogene 43 (43), 3184-3196 (2024)
   PUBMED   39261658
  REMARK    GeneRIF: ZG16 enhances the maturation of dendritic cells via
            induction of CD40 and contributes to the antitumor immunity in
            pancreatic cancer.
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Javitt,G., Kinzel,A., Reznik,N. and Fass,D.
  TITLE     Conformational switches and redox properties of the colon
            cancer-associated human lectin ZG16
  JOURNAL   FEBS J 288 (22), 6465-6475 (2021)
   PUBMED   34077620
  REMARK    GeneRIF: Conformational switches and redox properties of the colon
            cancer-associated human lectin ZG16.
REFERENCE   3  (bases 1 to 3116)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3116)
  AUTHORS   Meng,H., Li,W., Boardman,L.A. and Wang,L.
  TITLE     Loss of ZG16 is associated with molecular and clinicopathological
            phenotypes of colorectal cancer
  JOURNAL   BMC Cancer 18 (1), 433 (2018)
   PUBMED   29661177
  REMARK    GeneRIF: our study demonstrated that ZG16 expression was
            sequentially reduced from normal, adenoma, to carcinoma.
            Association with multiple clinicopathological features indicates
            that ZG16 may play an important role in cancer initiation and
            progression. ZG16 may serve as a potential biomarker for diagnosis
            and prognosis of colorectal cancer
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3116)
  AUTHORS   Chen,X., Du,P., She,J., Cao,L., Li,Y. and Xia,H.
  TITLE     Loss of ZG16 is regulated by miR-196a and contributes to stemness
            and progression of colorectal cancer
  JOURNAL   Oncotarget 7 (52), 86695-86703 (2016)
   PUBMED   27880730
  REMARK    GeneRIF: Data show that an upstream regulator of zymogen granule
            protein 16 (ZG16), miR-196a, which was significantly overexpressed
            in colorectal cancer (CRC) and promotes cell growth and stemness.
REFERENCE   6  (bases 1 to 3116)
  AUTHORS   Kanagawa,M., Satoh,T., Ikeda,A., Nakano,Y., Yagi,H., Kato,K.,
            Kojima-Aikawa,K. and Yamaguchi,Y.
  TITLE     Crystal structures of human secretory proteins ZG16p and ZG16b
            reveal a Jacalin-related beta-prism fold
  JOURNAL   Biochem Biophys Res Commun 404 (1), 201-205 (2011)
   PUBMED   21110947
  REMARK    GeneRIF: the sugar-binding site and the adjacent basic patch of
            ZG16p and ZG16b cooperatively form a functional
            glycosaminoglycan-binding site.
REFERENCE   7  (bases 1 to 3116)
  AUTHORS   Zhou,Y.B., Cao,J.B., Yang,H.M., Zhu,H., Xu,Z.G., Wang,K.S.,
            Zhang,X., Wang,Z.Q. and Han,Z.G.
  TITLE     hZG16, a novel human secreted protein expressed in liver, was
            down-regulated in hepatocellular carcinoma
  JOURNAL   Biochem Biophys Res Commun 355 (3), 679-686 (2007)
   PUBMED   17307141
  REMARK    GeneRIF: hZG16 was markedly down-regulated in hepatocellular
            carcinoma (HCC) as indicated by RT-PCR.
REFERENCE   8  (bases 1 to 3116)
  AUTHORS   Huang,Y.P., Cheng,J., Zhang,S.L., Wang,L., Guo,J., Liu,Y., Yang,Y.,
            Zhang,L.Y., Bai,G.Q., Gao,X.S., Ji,D., Lin,S.M. and Shao,Q.
  TITLE     Screening of hepatocyte proteins binding to F protein of hepatitis
            C virus by yeast two-hybrid system
  JOURNAL   World J Gastroenterol 11 (36), 5659-5665 (2005)
   PUBMED   16237761
REFERENCE   9  (bases 1 to 3116)
  AUTHORS   Zhang,Z. and Henzel,W.J.
  TITLE     Signal peptide prediction based on analysis of experimentally
            verified cleavage sites
  JOURNAL   Protein Sci 13 (10), 2819-2824 (2004)
   PUBMED   15340161
REFERENCE   10 (bases 1 to 3116)
  AUTHORS   Cronshagen,U., Voland,P. and Kern,H.F.
  TITLE     cDNA cloning and characterization of a novel 16 kDa protein located
            in zymogen granules of rat pancreas and goblet cells of the gut
  JOURNAL   Eur J Cell Biol 65 (2), 366-377 (1994)
   PUBMED   7720729
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC009133.7 and KF456182.1.
            
            On May 17, 2019 this sequence version replaced NM_152338.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK125559.1, BC150656.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000400752.6/ ENSP00000383563.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AC009133.7         87083-87133         c
            52-113              AC009133.7         86068-86129         c
            114-246             AC009133.7         85752-85884         c
            247-275             AC009133.7         85257-85285         c
            276-409             KF456182.1         1-134               c
            410-3116            AC009133.7         82416-85122         c
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p11.2"
     gene            1..3116
                     /gene="ZG16"
                     /gene_synonym="JCLN; JCLN1; ZG16A"
                     /note="zymogen granule protein 16"
                     /db_xref="GeneID:653808"
                     /db_xref="HGNC:HGNC:30961"
                     /db_xref="MIM:617311"
     exon            1..51
                     /gene="ZG16"
                     /gene_synonym="JCLN; JCLN1; ZG16A"
                     /inference="alignment:Splign:2.1.0"
     exon            52..113
                     /gene="ZG16"
                     /gene_synonym="JCLN; JCLN1; ZG16A"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..562
                     /gene="ZG16"
                     /gene_synonym="JCLN; JCLN1; ZG16A"
                     /note="jacalin-like lectin domain containing; zymogen
                     granule membrane protein 16; secretory lectin ZG16;
                     zymogen granule protein 16 homolog"
                     /codon_start=1
                     /product="zymogen granule membrane protein 16 precursor"
                     /protein_id="NP_689551.3"
                     /db_xref="CCDS:CCDS54000.1"
                     /db_xref="GeneID:653808"
                     /db_xref="HGNC:HGNC:30961"
                     /db_xref="MIM:617311"
                     /translation="MLTVALLALLCASASGNAIQARSSSYSGEYGGGGGKRFSHSGNQ
                     LDGPITALRVRVNTYYIVGLQVRYGKVWSDYVGGRNGDLEEIFLHPGESVIQVSGKYK
                     WYLKKLVFVTDKGRYLSFGKDSGTSFNAVPLHPNTVLRFISGRSGSLIDAIGLHWDVY
                     PSSCSRC"
     exon            114..246
                     /gene="ZG16"
                     /gene_synonym="JCLN; JCLN1; ZG16A"
                     /inference="alignment:Splign:2.1.0"
     exon            247..3116
                     /gene="ZG16"
                     /gene_synonym="JCLN; JCLN1; ZG16A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      638..643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZG16"
                     /gene_synonym="JCLN; JCLN1; ZG16A"
                     /note="hexamer: AATAAA"
     polyA_site      662
                     /gene="ZG16"
                     /gene_synonym="JCLN; JCLN1; ZG16A"
                     /note="major polyA site"
ORIGIN      
        1 ataaaacatc tggaagtttc cagggggctg ctttgcatct gaaactgtca gccccagaat
       61 gttgacagtc gctctcctag cccttctctg tgcctcagcc tctggcaatg ccattcaggc
      121 caggtcttcc tcctatagtg gagagtatgg aggtggtggt ggaaagcgat tctctcattc
      181 tggcaaccag ttggacggcc ccatcaccgc cctccgggtc cgagtcaaca catactacat
      241 cgtaggtctt caggtgcgct atggcaaggt gtggagcgac tatgtgggtg gtcgcaacgg
      301 agacctggag gagatctttc tgcaccctgg ggaatcagtg atccaggttt ctgggaagta
      361 caagtggtac ctgaagaagc tggtatttgt gacagacaag ggccgctatc tgtcttttgg
      421 gaaagacagt ggcacaagtt tcaatgccgt ccccttgcac cccaacaccg tgctccgctt
      481 catcagtggc cggtctggtt ctctcatcga tgccattggc ctgcactggg atgtttaccc
      541 cagtagctgc agcagatgct gagcctcctc tccttggcag gggcactgtg atgaggagta
      601 agaactccct tatcactaac ccccatccaa atggctcaat aaaaaaaata tggttaaggc
      661 tagtctgtgt gggggcatct gtggctggga tatctgcctc ctgacttagc cggggacgtg
      721 caaatctcac ttctggctgg ctttggacat ctgtctggaa gatgggaaga tgagggagag
      781 gtatgtaaga atcctgggct ttgtgctata atttatcaag aggagatgag attctggctt
      841 gcatcaacgc tcttcaagga cagctccttg gaacattgat ccaaactgga gtcatgggtc
      901 tgagggcaag gcctagttgt ggcttacacc aaaaccccag atgtcccact ctccagctct
      961 cctcacccct ggtcctcccc ttgagaaagt ggtgaactca cttgctgtgt gtgggtggcc
     1021 aggaccatta gcctttgttc tttcccagaa cccacctgac tcctgaaact tagctgaagt
     1081 ctgtgcccga ggaccctgcc ctgttaccag gcccagttcc tcctcacctc tacccatgag
     1141 ccccggtgtc ctgctaagcc ctctcagatc tgggattcct ccttcctcag gaagccacca
     1201 ccttctcagc agtggaaacc ctgcccacac tatgctctta ggctttagcc atcagaaggt
     1261 tacagtggac tgcgggaggc tgacactagg ctgaactcat taaggaatga atgggaggtg
     1321 agaagacaca ggcagcaaga atcgagtgtt tcaagaagtt tggctgtggt ttgccagaaa
     1381 taggcaagtc agttttcggg ggtgtaagga aaaagggttt tgtgtctttt taaaatccta
     1441 gacaggagag tcacaagcat gttcacatga taaagaggaa gaaagagaaa gaggctggag
     1501 attctgaaaa gagatcactg gtgaggtctc aaaagagata gaagaggatg gttatgtagt
     1561 tggggaaaga aattttaaga agggaagaaa attaaaatga gtgaaggtat acgttagttt
     1621 tgtaaaagtt atcaatatct ggctgggcac agtgctcaca cctgtaatcc cagcactttg
     1681 ggaggccaag gcaggcagat catttgaggt caggagttgg agacaagcct ccaacatggt
     1741 aaaaccctgt ctctactaaa aatacaaaaa ttagccaggt gtggtggcag tcacctgtaa
     1801 tcccagctac ttgggaggct gaggcatgag aatcacttga atgctggagg cagaggttac
     1861 aatgagttga gacagcacaa ctgcacccca gcctggatga cagagtgaga ctccatctaa
     1921 aaaaaaaagg aatgttatca gtatcaaaat ggagtaacat atgtcacatc ctaacaaaat
     1981 ggaaccaacc tgacacctaa attaagcatt gcacctgata acaccatgga ggactgggtg
     2041 gagaatcagc agacaatggc agggagaggc agtctgtgga ccaagttcag gtgaaaggat
     2101 cacccatcat gttgggcatc agatcaggca ggttcacaca ctacctgagg aagctggtct
     2161 ttgtgacaga caagagctgc tctgcctctt gggaaacaca ggcaccagtt ttagtgccat
     2221 cttcttgcac ccaaatgctg tcctttaact catcagttac agtgctggat ccctcatcac
     2281 tgccaatacg gggctagcaa tatgtctgcc ccagtgaatg cagccgccac ggagccccag
     2341 cctcctctat ggcaggggcc ctgtggtggg gaatgaggac ttctctatca ttaacctcca
     2401 tgcagttggc tcaagaaaag gatatggtag ttgggcaagg gggctcacac ctgtaatccc
     2461 agcacacttg taatcccagg agggtcgctt gagcccagga gtttaaggcc agcccgggca
     2521 acatagcaac ctgtttgtag gcaaaatgag gagttgaagt tccccgtagg atcttctagg
     2581 gctcaaccag ccttcatgtg cagtgggact ggcaagaaat ctgtcatccc tgttaccact
     2641 ttgaaggtct cagtgtgtat gcaggtcctg aaaagttcaa gtcccacctg aaacgcaaca
     2701 cagcatcatg ctctgattca tcagtgattc cctaatcaat gccattggct tgcattggga
     2761 ctatctatcc cagcctatcc agtgacttca gcagctgcag agccccagcc tcttctttgg
     2821 caggggcact gtggtgagga ggaaggattc ccttatcaca aatctccatc caattggttc
     2881 tataaacaga tatggtggcc caactgttgc gggaagtcag ggaccccgaa cggagggact
     2941 ggctggagcc acagctgagg aacataaatt gtgaagattt catggacatt taccagttcc
     3001 caaaattaat acttttatac tttcttatgc ctgtctttac tgcaatcact aaacataaat
     3061 tgtgaagatt tcatgaacat ttatcagttc ccaaataata ctcttataat ttctta
//
