LOCUS       NM_001284236           10805 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens zinc finger FYVE-type containing 16 (ZFYVE16),
            transcript variant 3, mRNA.
ACCESSION   NM_001284236 XM_005248631
VERSION     NM_001284236.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Liu,X., Salokas,K., Tamene,F., Jiu,Y., Weldatsadik,R.G., Ohman,T.
            and Varjosalo,M.
  TITLE     An AP-MS- and BioID-compatible MAC-tag enables comprehensive
            mapping of protein interactions and subcellular localizations
  JOURNAL   Nat Commun 9 (1), 1188 (2018)
   PUBMED   29568061
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10805)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 10805)
  AUTHORS   Shi,W., Chang,C., Nie,S., Xie,S., Wan,M. and Cao,X.
  TITLE     Endofin acts as a Smad anchor for receptor activation in BMP
            signaling
  JOURNAL   J Cell Sci 120 (Pt 7), 1216-1224 (2007)
   PUBMED   17356069
REFERENCE   5  (bases 1 to 10805)
  AUTHORS   Chen,Y.G., Wang,Z., Ma,J., Zhang,L. and Lu,Z.
  TITLE     Endofin, a FYVE domain protein, interacts with Smad4 and
            facilitates transforming growth factor-beta signaling
  JOURNAL   J Biol Chem 282 (13), 9688-9695 (2007)
   PUBMED   17272273
  REMARK    GeneRIF: Facilitates TGF-beta signaling as a scaffold protein to
            promote the R-Smad-Smad4 complex formation by bringing Smad4 to the
            proximity of the receptor complex.
REFERENCE   6  (bases 1 to 10805)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   7  (bases 1 to 10805)
  AUTHORS   Brill,L.M., Salomon,A.R., Ficarro,S.B., Mukherji,M.,
            Stettler-Gill,M. and Peters,E.C.
  TITLE     Robust phosphoproteomic profiling of tyrosine phosphorylation sites
            from human T cells using immobilized metal affinity chromatography
            and tandem mass spectrometry
  JOURNAL   Anal Chem 76 (10), 2763-2772 (2004)
   PUBMED   15144186
REFERENCE   8  (bases 1 to 10805)
  AUTHORS   Seet,L.F., Liu,N., Hanson,B.J. and Hong,W.
  TITLE     Endofin recruits TOM1 to endosomes
  JOURNAL   J Biol Chem 279 (6), 4670-4679 (2004)
   PUBMED   14613930
  REMARK    GeneRIF: marked recruitment of TOM1 to endosomes was observed in
            cells overexpressing endofin or its carboxyl-terminal fragment
REFERENCE   9  (bases 1 to 10805)
  AUTHORS   Seet,L.F. and Hong,W.
  TITLE     Endofin, an endosomal FYVE domain protein
  JOURNAL   J Biol Chem 276 (45), 42445-42454 (2001)
   PUBMED   11546807
REFERENCE   10 (bases 1 to 10805)
  AUTHORS   Xu,Y., Hortsman,H., Seet,L., Wong,S.H. and Hong,W.
  TITLE     SNX3 regulates endosomal function through its PX-domain-mediated
            interaction with PtdIns(3)P
  JOURNAL   Nat Cell Biol 3 (7), 658-666 (2001)
   PUBMED   11433298
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008771.4.
            
            On Nov 22, 2018 this sequence version replaced NM_001284236.2.
            
            Summary: This gene encodes an endosomal protein that belongs to the
            FYVE zinc finger family of proteins. The encoded protein is thought
            to regulate membrane trafficking in the endosome. This protein
            functions as a scaffold protein in the transforming growth
            factor-beta signaling pathway and is involved in positive and
            negative feedback regulation of the bone morphogenetic protein
            signaling pathway. Alternate splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2013].
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 ,3 and 5 encode the same protein
            (isoform a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR933621.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000505560.5/ ENSP00000426848.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-90                AC008771.4         14562-14651
            91-144              AC008771.4         33990-34043
            145-253             AC008771.4         40607-40715
            254-2505            AC008771.4         43254-45505
            2506-2602           AC008771.4         46434-46530
            2603-2764           AC008771.4         49621-49782
            2765-2907           AC008771.4         51761-51903
            2908-3286           AC008771.4         54524-54902
            3287-3409           AC008771.4         56089-56211
            3410-3565           AC008771.4         56929-57084
            3566-3790           AC008771.4         57983-58207
            3791-3873           AC008771.4         62190-62272
            3874-3978           AC008771.4         62959-63063
            3979-4126           AC008771.4         63443-63590
            4127-4207           AC008771.4         65912-65992
            4208-4370           AC008771.4         79259-79421
            4371-4476           AC008771.4         80252-80357
            4477-4644           AC008771.4         81161-81328
            4645-10805          AC008771.4         83717-89877
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q14.1"
     gene            1..10805
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /note="zinc finger FYVE-type containing 16"
                     /db_xref="GeneID:9765"
                     /db_xref="HGNC:HGNC:20756"
                     /db_xref="MIM:608880"
     exon            1..90
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            91..144
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            145..253
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..4803
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /note="isoform a is encoded by transcript variant 3;
                     endosome-associated FYVE-domain protein; protein
                     phosphatase 1, regulatory subunit 69; zinc finger FYVE
                     domain-containing protein 16; zinc finger, FYVE domain
                     containing 16"
                     /codon_start=1
                     /product="zinc finger FYVE domain-containing protein 16
                     isoform a"
                     /protein_id="NP_001271165.2"
                     /db_xref="CCDS:CCDS4050.1"
                     /db_xref="GeneID:9765"
                     /db_xref="HGNC:HGNC:20756"
                     /db_xref="MIM:608880"
                     /translation="MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVS
                     SELASSQRTSLLPKDQECVNSCASSETSYGTNESSLNEKTLKGLTSIQNEKNVTGLDL
                     LSSVDGGTSDEIQPLYMGRCSKPICDLISDMGNLVHATNSEEDIKKLLPDDFKSNADS
                     LIGLDLSSVSDTPCVSSTDHDSDTVREQQNDISSELQNREIGGIKELGIKVDTTLSDS
                     YNYSGTENLKDKKIFNQLESIVDFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVG
                     LVKEEVDVAVITAAECLKEEGKTSALTCSLPKNEDLCLNDSNSRDENFKLPDFSFQED
                     KTVIKQSAQEDSKSLDLKDNDVIQDSSSALHVSSKDVPSSLSCLPASGSMCGSLIESK
                     ARGDFLPQHEHKDNIQDAVTIHEEIQNSVVLGGEPFKENDLLKQEKCKSILLQSLIEG
                     MEDRKIDPDQTVIRAESLDGGDTSSTVVESQEGLSGTHVPESSDCCEGFINTFSSNDM
                     DGQDLDYFNIDEGAKSGPLISDAELDAFLTEQYLQTTNIKSFEENVNDSKSQMNQIDM
                     KGLDDGNINNIYFNAEAGAIGESHGINIICEIVDKQNTIENGLSLGEKSTIPVQQGLP
                     TSKSEITNQLSVSDINSQSVGGARPKQLFSLPSRTRSSKDLNKPDVPDTIESEPSTAD
                     TVVPITCAIDSTADPQVSFNSNYIDIESNSEGGSSFVTANEDSVPENTCKEGLVLGQK
                     QPTWVPDSEAPNCMNCQVKFTFTKRRHHCRACGKVFCGVCCNRKCKLQYLEKEARVCV
                     VCYETISKAQAFERMMSPTGSNLKSNHSDECTTVQPPQENQTSSIPSPATLPVSALKQ
                     PGVEGLCSKEQKRVWFADGILPNGEVADTTKLSSGSKRCSEDFSPLSPDVPMTVNTVD
                     HSHSTTVEKPNNETGDITRNEIIQSPISQVPSVEKLSMNTGNEGLPTSGSFTLDDDVF
                     AETEEPSSPTGVLVNSNLPIASISDYRLLCDINKYVCNKISLLPNDEDSLPPLLVASG
                     EKGSVPVVEEHPSHEQIILLLEGESFHPVTFVLNANLLVNVKFIFYSSDKYWYFSTNG
                     LHGLGQAEIIILLLCLPNEDTIPKDIFRLFITIYKDALKGKYIENLDNITFTESFLSS
                     KDHGGFLFITPTFQKLDDLSLPSNPFLCGILIQKLEIPWAKVFPMRLMLRLGAEYKAY
                     PAPLTSIRGRKPLFGEIGHTIMNLLVDLRNYQYTLHNIDQLLIHMEMGKSCIKIPRKK
                     YSDVMKVLNSSNEHVISIGASFSTEADSHLVCIQNDGIYETQANSATGHPRKVTGASF
                     VVFNGALKTSSGFLAKSSIVEDGLMVQITPETMNGLRLALREQKDFKITCGKVDAVDL
                     REYVDICWVDAEEKGNKGVISSVDGISLQGFPSEKIKLEADFETDEKIVKCTEVFYFL
                     KDQDLSILSTSYQFAKEIAMACSAALCPHLKTLKSNGMNKIGLRVSIDTDMVEFQAGS
                     EGQLLPQHYLNDLDSALIPVIHGGTSNSSLPLEIELVFFIIEHLF"
     exon            254..2505
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..2602
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            2603..2764
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..2907
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            2908..3286
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            3287..3409
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            3410..3565
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            3566..3790
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            3791..3873
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            3874..3978
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            3979..4126
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            4127..4207
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            4208..4370
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            4371..4476
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            4477..4644
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     exon            4645..10805
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6309..6314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /note="hexamer: AATAAA"
     polyA_site      6331
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /note="major polyA site"
     polyA_site      6567
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /note="major polyA site"
     regulatory      6660..6665
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /note="hexamer: AATAGA"
     polyA_site      6678
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
     regulatory      6752..6757
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /note="hexamer: AGTAAA"
     polyA_site      6776
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /note="major polyA site"
     regulatory      10785..10790
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
                     /note="hexamer: AATAAA"
     polyA_site      10805
                     /gene="ZFYVE16"
                     /gene_synonym="PPP1R69"
ORIGIN      
        1 actcccagga ctcccggccg gggtagctct tcactcctca gcgcgacgtc gtgtcgagtt
       61 cccaaaaagc tccgcagggg ctgtagggag attcctgtga ggtttctcgg gcttatttgg
      121 tggagtttct gttccagacc agaggcatac aagaattaaa ttctgaataa gtctgcaggt
      181 aggatggaca gttattttaa agcagctgtc agtgacttgg acaaactcct tgatgatttt
      241 gaacagaacc cagatgaaca agattatctc caagatgtac aaaatgcata tgattctaac
      301 cactgctcag tttcttcaga gttggcttcc tcacagcgaa cttcattgct cccaaaagac
      361 caagagtgcg ttaatagttg tgcctcatca gaaacaagct atggaacaaa tgagagttcc
      421 ctgaatgaaa aaacactcaa gggacttact tctatacaaa atgaaaaaaa tgtaacagga
      481 cttgatcttc tttcttctgt ggatggtggt acttcagatg aaatccagcc gttatatatg
      541 ggacgatgta gtaaacctat ctgtgatctg ataagtgaca tgggtaactt agttcatgca
      601 accaatagtg aagaagatat taaaaaatta ttgccagatg attttaagtc taatgcagat
      661 tccttgattg gattggattt atcttcagtg tcagatactc cctgtgtttc ttcaacagac
      721 catgatagtg atactgtcag agaacaacag aatgatatca gttctgaatt acaaaataga
      781 gaaatcggag gaatcaaaga attgggtata aaagtagata caacactttc agattcctat
      841 aattacagtg gaacagaaaa tttaaaagat aaaaagatct ttaatcagtt agaatcaatt
      901 gttgatttta acatgtcatc tgctttgact cgacaaagtt ccaaaatgtt tcatgccaaa
      961 gacaagctac aacacaagag ccagccatgt ggattactaa aagatgttgg cttagtaaaa
     1021 gaggaagtag atgtggcagt cataactgcc gcagaatgtt taaaagaaga gggcaagaca
     1081 agtgctttga cctgcagcct tccgaaaaat gaagatttat gcttaaatga ttcaaattca
     1141 agagatgaaa atttcaaatt acctgacttt tcctttcagg aagataagac tgttataaaa
     1201 caatctgcac aagaagactc aaaaagttta gaccttaagg ataatgatgt aatccaagat
     1261 tcctcttcag ctttacatgt ttccagtaaa gatgtgccgt cctcattgtc ctgtcttcct
     1321 gcgtctgggt ctatgtgtgg atcattaatt gaaagtaaag cacggggtga ttttttacct
     1381 cagcatgaac ataaagataa tatacaagat gcagtgacta tacatgaaga aatacagaac
     1441 agtgttgttc taggtgggga accattcaaa gagaatgatc ttttgaaaca ggaaaaatgt
     1501 aaaagcatac tccttcagtc attaattgaa gggatggaag acagaaagat agatcctgac
     1561 cagacagtaa tcagagctga gtctttggat ggtggtgaca ccagttctac agttgtagaa
     1621 tctcaagagg ggctttctgg cactcatgtc ccagagtctt ctgattgttg tgaaggtttt
     1681 attaatactt tttcaagcaa tgatatggat gggcaagact tagattactt taatattgat
     1741 gaaggcgcaa aaagtggccc actaattagt gatgctgaac ttgatgcctt tctgacagaa
     1801 cagtatcttc agaccactaa cataaagtct tttgaagaaa atgtaaatga ctctaaatcg
     1861 caaatgaatc agatagatat gaaaggctta gatgatggaa acatcaataa tatatatttc
     1921 aatgcagaag caggagctat tggggaaagt catggtatta atataatttg tgaaatagtt
     1981 gataaacaaa atacaataga aaatggcctt tctttaggag aaaaaagcac tattccagtt
     2041 caacaagggt tacctaccag taagtctgag attacaaatc aattatcggt ctctgatatt
     2101 aacagtcaat ctgttggagg ggccagacct aagcaattgt ttagccttcc atcaagaaca
     2161 aggagttcaa aggacctgaa taagccagat gttccagata caatagaaag tgaacccagc
     2221 acagcagata ccgttgttcc aatcacttgt gctatagatt ctacagctga tccacaggtt
     2281 agcttcaact ctaattacat tgatatagaa agtaattctg aaggtggatc tagtttcgta
     2341 actgcaaatg aagattctgt acctgaaaac acttgcaaag aaggcttggt tttgggccag
     2401 aaacagccta cttgggttcc tgattcagaa gctccaaact gtatgaactg ccaagtcaaa
     2461 tttactttta ccaaacggcg acaccattgc cgagcatgtg ggaaagtatt ttgtggtgtc
     2521 tgttgtaata ggaagtgtaa actgcaatat ctagaaaagg aagcaagagt atgtgtagtc
     2581 tgctatgaaa ctattagtaa agctcaggca tttgaaagga tgatgagtcc aactggttct
     2641 aatcttaagt ctaatcattc tgatgaatgt actactgtcc agcctcctca ggagaaccaa
     2701 acatccagta taccttcacc agcaactttg ccagtctcag cacttaaaca accaggtgtt
     2761 gaaggactat gttccaaaga acagaagaga gtatggtttg cagatggtat attgcccaat
     2821 ggtgaagttg cagatacaac aaaattatca tctggaagta aaagatgttc tgaagacttt
     2881 agtcctctct cacctgatgt gcctatgaca gtaaacacag tggatcattc ccattctact
     2941 acagtggaaa agccaaacaa tgagacagga gatattacaa gaaatgagat aattcagagt
     3001 cctatttctc aggttccatc agtggaaaaa ttgtctatga acacaggaaa tgaggggtta
     3061 cctacttctg gttcatttac actagatgat gatgtttttg cagaaactga agaaccatct
     3121 agtcctactg gtgtcttagt taacagcaat ttacctattg ctagtatttc agattatagg
     3181 ttactgtgtg atattaacaa gtatgtctgc aataagatta gtcttctacc taatgatgag
     3241 gacagtttgc ccccacttct ggttgcatct ggagaaaagg gatcagtgcc tgtagtagaa
     3301 gaacatccat ctcatgagca gatcattttg cttcttgaag gtgaaagctt tcatcctgtt
     3361 acatttgtcc taaatgctaa tctactcgtg aatgtcaaat tcatatttta ttcctcagac
     3421 aaatattggt acttttcaac caatggattg catggcttgg gacaggcaga aattattatt
     3481 ctattgttat gtttgccaaa tgaagatact attcctaagg acatcttcag actatttatc
     3541 accatatata aggatgctct aaaaggaaaa tacatagaaa acttggacaa tattaccttt
     3601 actgagagtt ttctcagtag caaggatcac ggaggattcc tgtttattac acctactttt
     3661 cagaaacttg atgatctctc attaccaagt aatccttttc tttgtggaat tcttatccag
     3721 aagcttgaga ttccctgggc aaaggttttt cctatgcgtt taatgttgag attgggtgca
     3781 gaatataaag catatcctgc tcctctaaca agcatcagag gccgaaaacc tctttttgga
     3841 gaaataggac acactattat gaacttactt gttgaccttc gaaattacca gtataccttg
     3901 cataatatag atcaactgtt gattcatatg gaaatgggaa aaagctgcat aaaaatacca
     3961 cggaaaaagt acagtgatgt aatgaaagta ctaaattctt ccaatgagca tgtcattagc
     4021 attggagcaa gtttcagtac agaagcagat tctcatctag tctgtataca gaatgatgga
     4081 atttatgaaa cacaggccaa cagtgccact ggccatccta gaaaagtgac aggtgcaagt
     4141 tttgtggtat tcaatggagc tctaaaaaca tcttcaggat ttcttgctaa gtccagcata
     4201 gttgaagatg gcttaatggt acaaataact ccagagacca tgaatggctt gcggctagct
     4261 ttacgagaac agaaagactt taaaattaca tgtgggaaag ttgatgcagt agacctgaga
     4321 gaatacgtgg atatctgctg ggtagatgct gaagaaaaag gaaacaaagg agttatcagt
     4381 tcagtggatg gaatatcatt acaaggattt ccaagtgaaa aaataaaact ggaagcagat
     4441 tttgaaaccg atgagaagat tgtaaaatgt accgaggtgt tctactttct aaaggaccag
     4501 gatttatcta ttttatcaac ttcttatcag tttgcaaaag aaatagccat ggcttgtagt
     4561 gctgcgctgt gccctcacct gaaaactcta aaaagtaatg ggatgaataa aattggactc
     4621 agagtttcca ttgacactga tatggttgaa tttcaggcag gatctgaagg ccaacttctg
     4681 cctcagcatt atctaaatga tcttgatagt gctctgatac ctgtgatcca tggtgggacc
     4741 tccaactcta gtttaccatt agaaatagaa ttagtgtttt tcattataga acatcttttt
     4801 tagtgaaaga atgtgccata ttacatattg caacctaatt tgttaaaact aactccagca
     4861 ctaaagctga aatgccacaa acactaaaag tataaatatg tctgattttt gaaacacata
     4921 agctttgctc tttaggcagg aatgatcttt tcaaatcatt agcacaatat ttaaatatct
     4981 aaaaatttaa gagatccata ctttctgtag ctttacaatt aatttaagta ctaaaaagac
     5041 aaggatttct tttaagaaat ttatagcatt tactgtgtta tttaaatgct aagccaaagt
     5101 atctgcactt aggtatacct ctttatgcca ataatgattt taatgaaggc tcttttcaga
     5161 tgtaacctta tgaaggaaat atctgctttg tgtatatgcc agttagaata ctggtttcta
     5221 aagtctgtca aattgtattt cagtggcaca aaaaccagtt ttgaggtctt agacttataa
     5281 ttctttgaat aaaactgata acttatttgt ataattggag tggagaccta cctccataat
     5341 tagataaact ctttttggat tataatcaga attttgcctt ttttcttctc aaattattac
     5401 atatgtatgt attatatatc cacatatata gttttccctg attaaatgga tattaaaata
     5461 attgcgggtg cttcaggact ttttgcttct atatttaagt atattgtttt tatagcaaga
     5521 acatattctg aatgttttat aaatctttaa taatttatat gtaggtaata tttttgtatc
     5581 acaatgcatt atttttttcc tcctttcctt ccaaactata ccactgtatt taccacttct
     5641 aagagtgact gacgacgggc cagatgaccc ttgaagtagt cattatgtag caataaatga
     5701 agcctgaaac aggttttttt acttccactt taatccttag aaatttcttg gcaacttcgc
     5761 atattttcat tgacaccagt gtataagtat aaatttaaat gaactaatta cttttgcata
     5821 ttttaaattc tttatatggt agttattttt tataacagga tattaacata agttaaatcc
     5881 tatgtatttg aaattgttac agagctttcc tctttacttc aaacagcaaa aaagtggggg
     5941 gcatattgta gtcctgtcat ttaagttatg taaaaaattt aatcattatt ttgatgcttt
     6001 aaacattctc atgtgtaata tatgtttttg tatcaaaaac actcatatat ttcaagaaaa
     6061 agaaattatg ttaaatagcc ctgttttaag aaaaatattt atgaagcatc tcaacttgaa
     6121 gatcaagtca aagttataac tcaggatctg aggtctcaag ctaggagaga ctgagaattt
     6181 taatcagttt gggcatatag tttggactga atcacatctg tagtacttag ccaaagacaa
     6241 tttggaggag aatatcagcc ttctggaagt agctacttcc tgaacaatgt aaagtgtcgc
     6301 agatattcaa taaaatggca acctgttata atttgtgaaa tttattgaaa tggtgtaaga
     6361 tgaaaacaat tgcatatcaa acccaattta tgttttctaa atatagtgta tgtattctgc
     6421 catgtaagta attgaacagt cttaaaataa ccaaatggta gagggctgtt ccatgatggg
     6481 acagctttgg atttgttttc ataaaatctc tatattcaat aaaaattgga attatgtgcc
     6541 tgaagtttgg aggcacattt tgaagtattc tttgtagaca tatagtatgt ttctgaatgt
     6601 gtttgttact tatttggtca ttggtactta atttgaaatt ttaatttttt aagagaagaa
     6661 atagaatggt tctttgtact cagtctgcaa tgatctattt ttggcttatg tcttttaata
     6721 ctactctctt tctctgaatt ttgaaatatg aagtaaaatc tagcccattt gttttatgca
     6781 gctattcaac aaactttgtg tgttcgtata cttgctacat gcagagcact ttaccaggtt
     6841 tctaagcaaa aatttctaaa aatgattaat tgttctgaca gaattaggcg tagttcttta
     6901 tcaacattta tggattccat gccacaagta gtatgcgctt aagtgcagag catgccaaga
     6961 gaaagagtag gatttggccc tgtaggagca aagattcaat tgtgggaaac tacttgtttc
     7021 tgaaaattgc atgctaggat atttacatca tgttcctgtt gaaaacaatt cctgattaaa
     7081 atacaagaat aatcttaaaa catcaaagag attttccaga caataaggaa tgccaagtcc
     7141 aaaagtaaag agaataagag aagcaagcat gtacccttac tctgacaacc ttatgagtga
     7201 gggggacaga aattaaagtt cagggcccat ataaggtgat gaacctaaca ggagaccctc
     7261 cacacaatag gctgagactc ccaaatactt tcacctttag gatgataatg aatcagaggt
     7321 ggaagaacca atgtaaaaga acaggcaagt ttagaattgc cccagtgatc taaagaatct
     7381 taagcactga gcttccttta aggtgttccc acactagtaa tgcctccagg tgacagagaa
     7441 aataaaatgt aaatactttt catggaaaag taatttcatt gtaggcctca aattactttt
     7501 caaatatgtc tagtaacaca tagtaaaaag tagtcatata aggaaactag attccagagg
     7561 caagaattac ttgaagcaat gaaaacaaac tcacagatat aggtattaga tttgttttct
     7621 ttgtttaagg aaataaggtg tgtgaatgta cttgcaggaa atgggaaact atacgataaa
     7681 aagttgtgga gctaatctgg aaaagaactg aatataaaca ttacaagaat atacataaaa
     7741 actgcacttt aaaactcagt aaatagatgt aatagaatgt agccaattgg ggtagtttat
     7801 aaactggaag ataactcagg agaaactatt cacagtgcac catgggagaa acagctggaa
     7861 aaaaaaacag aagacctgta gagaatacag tgaaaaatcc taagtgcttc attagctgtg
     7921 agaggagagg caagaatagg acagagcaaa tacttagata gaatgcagaa ttttaaaaac
     7981 tgatgaaaga catgacccca cacattctag aagccagaca aatcccaaac agaataaact
     8041 agaaaatcca cacatagaca cataataagg ccaggtgcag tggctcacac ctgtaatcga
     8101 gcactttggg aggccaaggc aggaggatca ctcgagctca ggttgagacc agagtgagca
     8161 acacagggag acactgtctc tgcaaaatta aaaaaatttt gccaggtgtg gtggctcata
     8221 cttgtggtcc cagcttcacg ggggctgagg tgggaggatc acttgagccc aggaggttaa
     8281 ggctccagtg agctgtgatc gtgcaacaga gcaaaacctt gtctcaaaaa aaaggaaaaa
     8341 gaaaaaagac atttaacagg aaaactggaa atgctagctg acagagagaa aaatagtcca
     8401 aggaacaatt tgctgtttct agtcagagga ccagaaagga ggttggaggc tatgggacaa
     8461 aacccttttt aatttaattg cagtattcta agagagcctc aggaaagatg cagtaagacc
     8521 caccgcccca ccaggagaac catgggtaat gacttggaac cttctggact ttccttgctc
     8581 tacatcaatc ccgcagcagt aaagaggtat cactcttctc ttccccctgg tgcactagtg
     8641 gcagacattg cagggcagaa cactggcaat ggggaggcta acgccctgac tttctagcca
     8701 aaaaccaaga atggggttct agaaaccaga gtataggtaa gatcacaaat aggagggaac
     8761 tgaagaaaag gaacccttaa atctgtcaat aaaatcctgg gatcatctca caagctgtgt
     8821 atgtgtgaaa ctaaccagaa gcagctgagc aaaggttctg aacactgaat tacagtgtag
     8881 gccactgtcc aagtttcaga ttggccacta gatggtgcac aactggggca aagaacagct
     8941 taacaaaggc agtgaaaact aacattggaa ccacagccac caaaaaaagt ggccaggatg
     9001 tgtgctctga aacttaactg aattgatggt ctgctaaaac aaaacaaaac aaaaacattc
     9061 ttatgaagat ttaaacagga tagagaatat cataatattc caaatatatc ccagaaacaa
     9121 cccaaaatga ttggacaaag aacttgtgaa acctaagcaa ctctctaggg aaaagacaat
     9181 gaacaagagc caactctaag gttacccaaa tgtcagaatt atcagaaaaa gacttaaaac
     9241 tagttatttt atttttttta tttatttttg agacagtctc tatcacccag gctagagtgc
     9301 agtgatacaa tcttggctca ctgcaacctc cacccccagg gtgcaagtga ttctcctgtc
     9361 tcagcctcct gagtagctgt gactacaggt gtgtaccacc acgcctagat aattttttgt
     9421 atttttaata gagacagagt ttcaccacgt tggccaggct ggtctcaaac tcctgacctc
     9481 aagtgatctg cctgcctggg cctcccaaag tgctgggatt acaggcataa gccagcacac
     9541 ctggccgtta aactagttat tttaatcatg ctacacaaag aagacatttc tccaaaggaa
     9601 atatataaag ggaaacactg ttgaaataaa tggaaagcta aaagtctcag catagaagct
     9661 ataaaaagaa acaagtggaa attgtagaac tgaaaataca ataacaaaat ttactggaag
     9721 gtctcaacac aatgaagatg actgagggaa gagtcagtaa atttgaagat acatgagcag
     9781 aaattatcta gtctgaatga cagagaaaaa agtgaaaaaa atgagaagac ctttggggat
     9841 ctgtgggaca atatgaaaca gtctaatatt catgtcttag gagcccagaa agaacaattg
     9901 gaagaaaaaa atatctgaag aaataatagc taaaaacttc caaacatctg gcaaaaaaca
     9961 aatattcaca tacatttaca tagcattcta tttaaagccg gtctgctggc acagcaaatc
    10021 ccaaaaagga aaaccacacc caggcacgtc ataatcaaag tgatgaaacc aactgtaaag
    10081 aaaatacttt gaaaacaact ggaagaaaac aacactttgt atataggaga accaaagatt
    10141 tcagtgatgg cagatttctc atcagaaacc atggagcaac atttataaag tgcttttaaa
    10201 aagcaacaaa actgtcaaca cagaatcctt tatccagcaa atatattctt taggaaagaa
    10261 aatgaaataa agacattttc ttttttttct ttactttttt tttttttatg agatggagtc
    10321 tcactctgtt gcccaggctg gaatggattg gtgcgatctc agctcactgc aacctccgcc
    10381 tcccggttca agcagttctc ctgtctgagc ctccagagga gtagctggga ttacaggtgc
    10441 gtggtaccac gcccagctaa tttttttatg tttgtagtag agacgagttt caccatgttg
    10501 gtcaggatgg tctcaaactc ctgacctcag gtgatctgcc tgcttcggcc tcccaaagtg
    10561 ctgagattag aggcatgagc caccatacct ggctgaaata aagacattgt cagaagaagg
    10621 aaagctaaaa taattcattg ctagtagtcc tcctttaaca gaaatggtaa aggaagttca
    10681 tggggaagga aaatgattgc aaagaaggaa gagcaaccaa aatggcaaat ctctggataa
    10741 accaaataga cttacatgtt tgttaaaata tatgtaactg ctgaaataaa aaaattgtaa
    10801 catca
//
