LOCUS       NM_024721              13987 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens zinc finger homeobox 4 (ZFHX4), transcript variant 1,
            mRNA.
ACCESSION   NM_024721
VERSION     NM_024721.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13987)
  AUTHORS   Zong,S., Xu,P.P., Xu,Y.H. and Guo,Y.
  TITLE     A bioinformatics analysis: ZFHX4 is associated with metastasis and
            poor survival in ovarian cancer
  JOURNAL   J Ovarian Res 15 (1), 90 (2022)
   PUBMED   35915456
  REMARK    GeneRIF: A bioinformatics analysis: ZFHX4 is associated with
            metastasis and poor survival in ovarian cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13987)
  AUTHORS   Ha,M., Kim,J., Park,S.M., Hong,C.M., Han,M.E., Song,P., Kang,C.D.,
            Lee,D., Kim,Y.H., Hur,J. and Oh,S.O.
  TITLE     Prognostic Role of Zinc Finger Homeobox 4 in Ovarian Serous
            Cystadenocarcinoma
  JOURNAL   Genet Test Mol Biomarkers 24 (3), 145-149 (2020)
   PUBMED   32105524
  REMARK    GeneRIF: Prognostic Role of Zinc Finger Homeobox 4 in Ovarian
            Serous Cystadenocarcinoma.
REFERENCE   3  (bases 1 to 13987)
  AUTHORS   Qing,T., Zhu,S., Suo,C., Zhang,L., Zheng,Y. and Shi,L.
  TITLE     Somatic mutations in ZFHX4 gene are associated with poor overall
            survival of Chinese esophageal squamous cell carcinoma patients
  JOURNAL   Sci Rep 7 (1), 4951 (2017)
   PUBMED   28694483
  REMARK    GeneRIF: Results showed that ZFHX4 was overexpressed in esophageal
            squamous cell carcinoma (ESCC) tumor compared to normal controls,
            and knockdown of ZFHX4 in vitro significantly inhibited cell
            migration and invasion. Mutations in ZFHX4 were strongly associated
            with poor prognosis and its down-regulation inhibits the
            progression of ESCC.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 13987)
  AUTHORS   Stacey,S.N., Helgason,H., Gudjonsson,S.A., Thorleifsson,G.,
            Zink,F., Sigurdsson,A., Kehr,B., Gudmundsson,J., Sulem,P.,
            Sigurgeirsson,B., Benediktsdottir,K.R., Thorisdottir,K.,
            Ragnarsson,R., Fuentelsaz,V., Corredera,C., Gilaberte,Y., Grasa,M.,
            Planelles,D., Sanmartin,O., Rudnai,P., Gurzau,E., Koppova,K.,
            Nexo,B.A., Tjonneland,A., Overvad,K., Jonasson,J.G.,
            Tryggvadottir,L., Johannsdottir,H., Kristinsdottir,A.M.,
            Stefansson,H., Masson,G., Magnusson,O.T., Halldorsson,B.V.,
            Kong,A., Rafnar,T., Thorsteinsdottir,U., Vogel,U., Kumar,R.,
            Nagore,E., Mayordomo,J.I., Gudbjartsson,D.F., Olafsson,J.H. and
            Stefansson,K.
  TITLE     New basal cell carcinoma susceptibility loci
  JOURNAL   Nat Commun 6, 6825 (2015)
   PUBMED   25855136
  REMARK    GeneRIF: rs28727938[G] is associated with susceptibility to
            cutaneous basal cell carcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 13987)
  AUTHORS   Chudnovsky,Y., Kim,D., Zheng,S., Whyte,W.A., Bansal,M., Bray,M.A.,
            Gopal,S., Theisen,M.A., Bilodeau,S., Thiru,P., Muffat,J.,
            Yilmaz,O.H., Mitalipova,M., Woolard,K., Lee,J., Nishimura,R.,
            Sakata,N., Fine,H.A., Carpenter,A.E., Silver,S.J., Verhaak,R.G.,
            Califano,A., Young,R.A., Ligon,K.L., Mellinghoff,I.K., Root,D.E.,
            Sabatini,D.M., Hahn,W.C. and Chheda,M.G.
  TITLE     ZFHX4 interacts with the NuRD core member CHD4 and regulates the
            glioblastoma tumor-initiating cell state
  JOURNAL   Cell Rep 6 (2), 313-324 (2014)
   PUBMED   24440720
  REMARK    GeneRIF: ZFHX4 interacts with and regulates CHD4.
REFERENCE   6  (bases 1 to 13987)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   7  (bases 1 to 13987)
  AUTHORS   Gudbjartsson,D.F., Walters,G.B., Thorleifsson,G., Stefansson,H.,
            Halldorsson,B.V., Zusmanovich,P., Sulem,P., Thorlacius,S.,
            Gylfason,A., Steinberg,S., Helgadottir,A., Ingason,A.,
            Steinthorsdottir,V., Olafsdottir,E.J., Olafsdottir,G.H.,
            Jonsson,T., Borch-Johnsen,K., Hansen,T., Andersen,G., Jorgensen,T.,
            Pedersen,O., Aben,K.K., Witjes,J.A., Swinkels,D.W., den Heijer,M.,
            Franke,B., Verbeek,A.L., Becker,D.M., Yanek,L.R., Becker,L.C.,
            Tryggvadottir,L., Rafnar,T., Gulcher,J., Kiemeney,L.A., Kong,A.,
            Thorsteinsdottir,U. and Stefansson,K.
  TITLE     Many sequence variants affecting diversity of adult human height
  JOURNAL   Nat Genet 40 (5), 609-615 (2008)
   PUBMED   18391951
REFERENCE   8  (bases 1 to 13987)
  AUTHORS   Hemmi,K., Ma,D., Miura,Y., Kawaguchi,M., Sasahara,M.,
            Hashimoto-Tamaoki,T., Tamaoki,T., Sakata,N. and Tsuchiya,K.
  TITLE     A homeodomain-zinc finger protein, ZFHX4, is expressed in neuronal
            differentiation manner and suppressed in muscle differentiation
            manner
  JOURNAL   Biol Pharm Bull 29 (9), 1830-1835 (2006)
   PUBMED   16946494
REFERENCE   9  (bases 1 to 13987)
  AUTHORS   McMullan,T.W., Crolla,J.A., Gregory,S.G., Carter,N.P., Cooper,R.A.,
            Howell,G.R. and Robinson,D.O.
  TITLE     A candidate gene for congenital bilateral isolated ptosis
            identified by molecular analysis of a de novo balanced
            translocation
  JOURNAL   Hum Genet 110 (3), 244-250 (2002)
   PUBMED   11935336
  REMARK    GeneRIF: Human ZFH-4 is a candidate gene for congenital bilateral
            isolated ptosis, identified by molecular analysis of a de novo
            balanced translocation.
REFERENCE   10 (bases 1 to 13987)
  AUTHORS   Sakata,N., Hemmi,K., Kawaguchi,M., Miura,Y., Noguchi,S., Ma,D.,
            Sasahara,M., Kato,T., Hori,M. and Tamaoki,T.
  TITLE     The mouse ZFH-4 protein contains four homeodomains and twenty-two
            zinc fingers
  JOURNAL   Biochem Biophys Res Commun 273 (2), 686-693 (2000)
   PUBMED   10873665
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC011716.7, DA658176.1, AB083343.2, AC087110.3, AC023200.6 and
            CA430749.1.
            
            On May 17, 2019 this sequence version replaced NM_024721.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1968968,
                              SAMEA2145245 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000651372.2/ ENSP00000498627.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                AC011716.7         112352-112384
            34-41               DA658176.1         1-8
            42-3806             AB083343.2         1-3765
            3807-3807           AC087110.3         28254-28254         c
            3808-6541           AB083343.2         3767-6500
            6542-8178           AC087110.3         6974-8610           c
            8179-10706          AB083343.2         8087-10614
            10707-13295         AC023200.6         88002-90590         c
            13296-13987         CA430749.1         22-713              c
FEATURES             Location/Qualifiers
     source          1..13987
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q21.13"
     gene            1..13987
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /note="zinc finger homeobox 4"
                     /db_xref="GeneID:79776"
                     /db_xref="HGNC:HGNC:30939"
                     /db_xref="MIM:606940"
     exon            1..374
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     exon            375..3010
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     CDS             421..11271
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /note="isoform 1 is encoded by transcript variant 1; zinc
                     finger homeobox protein 4; zinc-finger homeodomain protein
                     4"
                     /codon_start=1
                     /product="zinc finger homeobox protein 4 isoform 1"
                     /protein_id="NP_078997.4"
                     /db_xref="CCDS:CCDS47878.2"
                     /db_xref="GeneID:79776"
                     /db_xref="HGNC:HGNC:30939"
                     /db_xref="MIM:606940"
                     /translation="METCDSPPISRQENGQSTSKLCGTTQLDNEVPEKVAGMEPDREN
                     SSTDDNLKTDERKSEALLGFSVENAAATQVTSAKEIPCNECATSFPSLQKYMEHHCPN
                     ARLPVLKDDNESEISELEDSDVENLTGEIVYQPDGSAYIIEDSKESGQNAQTGANSKL
                     FSTAMFLDSLASAGEKSDQSASAPMSFYPQIINTFHIASSLGKPFTADQAFPNTSALA
                     GVGPVLHSFRVYDLRHKREKDYLTSDGSAKNSCVSKDVPNNVDLSKFDGCVSDGKRKP
                     VLMCFLCKLSFGYIRSFVTHAVHDHRMTLNDEEQKLLSNKCVSAIIQGIGKDKEPLIS
                     FLEPKKSTSVYPHFSTTNLIGPDPTFRGLWSAFHVENGDSLPAGFAFLKGSASTSSSA
                     EQPLGITQMPKAEVNLGGLSSLVVNTPITSVSLSHSSSESSKMSESKDQENNCERPKE
                     SNVLHPNGECPVKSEPTEPGDEDEEDAYSNELDDEEVLGELTDSIGNKDFPLLNQSIS
                     PLSSSVLKFIEKGTSSSSATVSDDTEKKKQTAAVRASGSVASNYGISGKDFADASASK
                     DSATAAHPSEIARGDEDSSATPHQHGFTPSTPGTPGPGGDGSPGSGIECPKCDTVLGS
                     SRSLGGHMTMMHSRNSCKTLKCPKCNWHYKYQQTLEAHMKEKHPEPGGSCVYCKTGQP
                     HPRLARGESYTCGYKPFRCEVCNYSTTTKGNLSIHMQSDKHLNNVQNLQNGNGEQVFG
                     HSAPAPNTSLSGCGTPSPSKPKQKPTWRCEVCDYETNVARNLRIHMTSEKHMHNMMLL
                     QQNMKQIQHNLHLGLAPAEAELYQYYLAQNIGLTGMKLENPADPQLMINPFQLDPATA
                     AALAPGLVNNELPPEIRLASGQLMGDDLSLLTAGELSPYISDPALKLFQCAVCNKFTS
                     DSLEALSVHVSSERSLPEEEWRAVIGDIYQCKLCNYNTQLKANFQLHCKTDKHMQKYQ
                     LVAHIKEGGKSNEWRLKCIAIGNPVHLKCNACDYYTNSVDKLRLHTTNHRHEAALKLY
                     KHLQKQEGAVNPESCYYYCAVCDYTTKVKLNLVQHVRSVKHQQTEGLRKLQLHQQGLA
                     PEEDNLSEIFFVKDCPPNELETASLGARTCDDDLTEQQLRSTSEEQSEEAEGAIKPTA
                     VAEDDEKDTSERDNSEGKNSNKDSGIITPEKELKVSVAGGTQPLLLAKEEDVATKRSK
                     PTEDNKFCHEQFYQCPYCNYNSRDQSRIQMHVLSQHSVQPVICCPLCQDVLSNKMHLQ
                     LHLTHLHSVSPDCVEKLLMTVPVPDVMMPNSMLLPAAASEKSERDTPAAVTAEGSGKY
                     SGESPMDDKSMAGLEDSKANVEVKNEEQKPTKEPLEVSEWNKNSSKDVKIPDTLQDQL
                     NEQQKRQPLSVSDRHVYKYRCNHCSLAFKTMQKLQIHSQYHAIRAATMCNLCQRSFRT
                     FQALKKHLEAGHPELSEAELQQLYASLPVNGELWAESETMSQDDHGLEQEMEREYEVD
                     HEGKASPVGSDSSSIPDDMGSEPKRTLPFRKGPNFTMEKFLDPSRPYKCTVCKESFTQ
                     KNILLVHYNSVSHLHKLKKVLQEASSPVPQETNSNTDNKPYKCSICNVAYSQSSTLEI
                     HMRSVLHQTKARAAKLEPSGHVAGGHSIAANVNSPGQGMLDSMSLAAVNSKDTHLDAK
                     ELNKKQTPDLISAQPAHHPPQSPAQIQMQLQHELQQQAAFFQPQFLNPAFLPHFPMTP
                     EALLQFQQPQFLFPFYIPGTEFSLGPDLGLPGSATFGMPGMTGMAGSLLEDLKQQIQT
                     QHHVGQTQLQILQQQAQQYQATQPQLQPQKQQQQPPPPQQQQQQQASKLLKQEQSNIV
                     SADCQIMKDVPSYKEAEDISEKPEKPKQEFISEGEGLKEGKDTKKQKSLEPSIPPPRI
                     ASGARGNAAKALLENFGFELVIQYNENRQKVQKKGKSGEGENTDKLECGTCGKLFSNV
                     LILKSHQEHVHGQFFPYAALEKFARQYREAYDKLYPISPSSPETPPPPPPPPPLPPAP
                     PQPSSMGPVKIPNTVSTPLQAPPPTPPPPPPPPPPPPPPPPPPPPSAPPQVQLPVSLD
                     LPLFPSIMMQPVQHPALPPQLALQLPQMDALSADLTQLCQQQLGLDPNFLRHSQFKRP
                     RTRITDDQLKILRAYFDINNSPSEEQIQEMAEKSGLSQKVIKHWFRNTLFKERQRNKD
                     SPYNFSNPPITVLEDIRIDPQPTSLEHYKSDASFSKRSSRTRFTDYQLRVLQDFFDTN
                     AYPKDDEIEQLSTVLNLPTRVIVVWFQNARQKARKSYENQAETKDNEKRELTNERYIR
                     TSNMQYQCKKCNVVFPRIFDLITHQKKQCYKDEDDDAQDESQTEDSMDATDQVVYKHC
                     TVSGQTDAAKNAAAPAASSGSGTSTPLIPSPKPEPEKTSPKPEYPAEKPKQSDPSPPS
                     QGTKPALPLASTSSDPPQASTAQPQPQPQPPKQPQLIGRPPSASQTPVPSSPLQISMT
                     SLQNSLPPQLLQYQCDQCTVAFPTLELWQEHQHMHFLAAQNQFLHSPFLERPMDMPYM
                     IFDPNNPLMTGQLLGSSLTQMPPQASSSHTTAPTTVAASLKRKLDDKEDNNCSEKEGG
                     NSGEDQHRDKRLRTTITPEQLEILYEKYLLDSNPTRKMLDHIAREVGLKKRVVQVWFQ
                     NTRARERKGQFRAVGPAQSHKRCPFCRALFKAKSALESHIRSRHWNEGKQAGYSLPPS
                     PLISTEDGGESPQKYIYFDYPSLPLTKIDLSSENELASTVSTPVSKTAELSPKNLLSP
                     SSFKAECSEDVENLNAPPAEAGYDQNKTDFDETSSINTAISDATTGDEGNTEMESTTG
                     SSGDVKPALSPKEPKTLDTLPKPATTPTTEVCDDKFLFSLTSPSIHFNDKDGDHDQSF
                     YITDDPDDNADRSETSSIADPSSPNPFGSSNPFKSKSNDRPGHKRFRTQMSNLQLKVL
                     KACFSDYRTPTMQECEMLGNEIGLPKRVVQVWFQNARAKEKKFKINIGKPFMINQGGT
                     EGTKPECTLCGVKYSARLSIRDHIFSKQHISKVRETVGSQLDREKDYLAPTTVRQLMA
                     QQELDRIKKASDVLGLTVQQPGMMDSSSLHGISLPTAYPGLPGLPPVLLPGMNGPSSL
                     PGFPQNSNTLTPPGAGMLGFPTSATSSPALSLSSAPTKPLLQTPPPPPPPPPPPPSSS
                     LSGQQTEQQNKESEKKQTKPNKVKKIKEEELEATKPEKHPKKEEKISSALSVLGKVVG
                     ETHVDPIQLQALQNAIAGDPASFIGGQFLPYFIPGFASYFTPQLPGTVQGGYFPPVCG
                     MESLFPYGPTMPQTLAGLSPGALLQQYQQYQQNLQESLQKQQKQQQEQQQKPVQAKTS
                     KVESDQPQNSNDASETKEDKSTATESTKEEPQLESKSADFSDTYVVPFVKYEFICRKC
                     QMMFTDEDAAVNHQKSFCYFGQPLIDPQETVLRVPVSKYQCLACDVAISGNEALSQHL
                     QSSLHKEKTIKQAMRNAKEHVRLLPHSVCSPNPNTTSTSQSAASSNNTYPHLSCFSMK
                     SWPNILFQASARRAASPPSSPPSLSLPSTVTSSLCSTSGVQTSLPTESCSDESDSELS
                     QKLEDLDNSLEVKAKPASGLDGNFNSIRMDMFSV"
     exon            3011..3513
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     exon            3514..3745
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     exon            3746..3814
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     exon            3815..3931
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     exon            3932..4065
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     exon            4066..4266
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     exon            4267..4384
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     exon            4385..9799
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     exon            9800..13987
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      13967..13972
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /note="hexamer: ATTAAA"
     polyA_site      13987
                     /gene="ZFHX4"
                     /gene_synonym="ZFH4; ZHF4"
                     /note="major polyA site"
ORIGIN      
        1 acacaaagac atgcgcactc aacttaatca gccatttttt taaacagggc taaaacgata
       61 ataattagca gaataaagac atatcggatt ttcatttcct ttcctccttt tcccaacccc
      121 ttcacaacca aacagcgaga ccgcggtcgg cacatgcttt aactcctccc ggacccccga
      181 ggaccgctcc atgcccccca ctttctgctc cagcgttttt attttcaccc aataaagttc
      241 gaggattatt ttttattttt tttgtttttt taatgaaccc tctcgtttta cttggatgtg
      301 atcagctgta agtaaaataa aagcaaaaca aaaaagaggc gaagatcgag taggaactgc
      361 aggggaaatg gaaagtccct gacaggctgg atgaaatgag atccccatgt agcaattgcc
      421 atggaaacct gtgactcccc tcctatctca aggcaggaaa atgggcagag cacatcaaag
      481 ctatgtggaa cgacacaact tgataatgag gtgccagaga aagttgcagg gatggagcct
      541 gacagggaaa acagctccac agatgacaac ctgaaaacgg atgagcgcaa aagtgaagcc
      601 ttgctgggtt tcagcgttga gaatgcagct gccactcagg ttacctcagc aaaggagata
      661 ccctgcaacg aatgtgccac ttcttttccc agtttacaga aatacatgga acaccactgc
      721 cctaatgccc gccttcctgt cctgaaggat gacaacgaga gcgagatcag cgagttagag
      781 gacagtgacg tggaaaatct aacaggggag atcgtttacc agcctgatgg gtcagcatat
      841 ataattgagg actccaaaga aagtgggcag aatgcacaga ctggggcaaa tagcaaactc
      901 ttttctacag cgatgttcct ggactccctg gcatctgctg gagagaagag tgatcagtct
      961 gcttctgcac ctatgtcgtt ctacccacag atcatcaaca cttttcatat cgcttcatcc
     1021 ctcgggaaac catttacagc cgatcaggct ttcccaaata cctcagcatt agcaggagtt
     1081 ggtcctgtgt tgcacagttt ccgtgtctat gatctccgac acaagagaga gaaagactat
     1141 ctaaccagtg atggctcagc caaaaactcc tgtgtgtcca aagatgtccc taacaatgtg
     1201 gacttgtcca aattcgatgg ttgtgttagc gatgggaaaa ggaaacctgt tttaatgtgt
     1261 ttcttgtgca agttgtcttt tggttatatc aggtcatttg taacccatgc tgtgcatgat
     1321 catcggatga ccctcaatga cgaggagcag aagctcctca gtaataaatg cgtctccgcc
     1381 ataatacagg ggattggcaa agacaaagaa cctcttataa gctttctgga accaaaaaaa
     1441 tccacttctg tttatcccca tttttctact acaaacctca taggacccga tccaaccttc
     1501 cgcggtttat ggagcgcttt tcatgttgaa aatggtgact ctttgccggc tggctttgcc
     1561 ttcttaaaag gaagcgcgag cacctcgagc tcagcagagc agccgctggg gattacccaa
     1621 atgccaaagg ctgaagtgaa tctggggggg ctgtctagtt tagtagtgaa caccccaatt
     1681 acctctgtct ccctcagcca ctcatcgtct gagtctagca agatgtcaga gagcaaagac
     1741 caagagaaca actgtgaaag gccaaaagaa agcaacgttt tacacccaaa cggggagtgc
     1801 cctgtcaaaa gtgaacccac tgaaccggga gatgaggatg aagaagatgc gtactccaat
     1861 gaacttgatg acgaggaagt attaggtgaa ctcaccgata gtattggtaa caaagatttc
     1921 cctctcttaa accaaagcat ttctccttta tcatccagtg tgctaaaatt tattgaaaag
     1981 ggtacctcgt cctcctcggc gactgtttct gatgacacag aaaagaaaaa acagactgct
     2041 gctgttaggg ccagtggcag tgttgctagt aactatggca tcagtggcaa ggactttgca
     2101 gacgcaagtg ccagtaaaga cagtgccaca gctgctcatc caagtgaaat agcccgggga
     2161 gacgaagaca gttcagccac tcctcaccag catggcttta ccccgagtac tcctggcaca
     2221 ccagggcctg gaggagacgg ctcaccgggc agtggcatcg agtgtccaaa gtgcgacact
     2281 gtgttggggt cttcgaggtc tcttggtggt catatgacta tgatgcactc gaggaactca
     2341 tgcaaaaccc tcaaatgtcc taaatgtaac tggcactaca aatatcagca gaccctggag
     2401 gcccatatga aggagaaaca ccctgagccg ggtggctctt gtgtttattg taagactgga
     2461 cagcctcacc ccaggcttgc ccggggtgag agttacacgt gtggctataa acccttccgt
     2521 tgtgaggttt gtaactactc taccactacc aaaggcaacc tcagtattca tatgcagtcg
     2581 gacaagcacc tgaacaatgt tcagaatctc caaaatggca atggtgagca ggtgtttggc
     2641 cactctgccc cagcccccaa caccagcctc agtggctgcg gaacaccctc tccgtccaaa
     2701 cccaaacaga aacccacctg gcggtgtgaa gtttgtgatt atgaaaccaa tgtcgccagg
     2761 aacctccgaa ttcatatgac cagcgaaaag cacatgcata atatgatgct tttgcagcag
     2821 aacatgaagc agatccagca taatctgcac ttgggcctcg ccccggcgga agcagagctt
     2881 tatcagtact acctagccca gaacataggc ctgaccggaa tgaagctgga aaaccctgcc
     2941 gaccctcagc tgatgatcaa tccattccag ctggatccag cgacagcagc ggctttggca
     3001 ccagggctcg taaataatga gctgccgcct gaaatccggc ttgccagtgg tcagctaatg
     3061 ggtgatgacc tgtccctcct tactgcagga gagctgtcac cttatatcag tgacccagcg
     3121 ctgaagctat tccagtgtgc tgtttgcaac aaattcacct ctgacagcct ggaggcccta
     3181 agtgtgcatg tgagcagtga gcgctctctc cctgaagagg aatggagggc agtaattgga
     3241 gatatctacc agtgcaagct ctgcaactac aacactcagc tcaaagccaa cttccagcta
     3301 cactgcaaga ctgataaaca tatgcagaaa tatcaactgg tggctcacat taaagaaggg
     3361 ggcaaaagca atgagtggag gttgaagtgt attgccattg gcaaccctgt tcacctaaaa
     3421 tgtaacgcct gtgactatta caccaacagt gtggataaat tacgcttgca taccaccaat
     3481 cacaggcacg aggcggccct gaagctctac aagcacttgc agaagcaaga gggtgcagtg
     3541 aatcccgaat cctgctatta ctactgtgcc gtgtgtgatt acaccaccaa ggtcaagttg
     3601 aatctggtac aacatgtccg ttcggtgaag catcagcaga ctgagggcct acggaagctc
     3661 cagctccacc agcaaggcct ggcaccagag gaggacaacc tcagtgagat cttttttgtt
     3721 aaagattgcc caccaaatga gcttgaaact gcctcattgg gagccaggac ttgtgatgat
     3781 gatcttacag agcagcagtt gagatcgacc tcagaagaac aaagtgagga ggcagaagga
     3841 gctattaagc ctacagcagt ggccgaggac gatgaaaaag acacaagtga gagagacaat
     3901 agtgaaggca aaaactctaa taaagactct gggataatca caccagagaa ggaactaaaa
     3961 gttagtgtgg cagggggtac ccagccactc ctgctggcaa aagaagagga tgttgcaaca
     4021 aaaaggtcaa aacctacaga ggacaataaa ttctgtcatg aacagttcta tcaatgtcct
     4081 tattgtaact acaatagtag ggaccaaagt cgtatccaga tgcacgtcct atcacagcac
     4141 tcggtgcagc cggtcatctg ctgtcctctc tgtcaggacg tcctcagcaa caaaatgcat
     4201 ctccaactgc atctgacgca tttgcacagt gtgtctccag actgtgtgga gaagctgctt
     4261 atgacagtgc ctgtccctga tgtgatgatg ccaaacagta tgctactgcc agcagctgcc
     4321 tctgagaaat cagagcggga cacacctgca gccgtgacag ctgaggggtc tgggaaatat
     4381 tcaggtgaaa gtccaatgga tgacaaaagc atggcaggtc tcgaggattc aaaggctaat
     4441 gtggaagtaa agaatgagga gcagaaaccg actaaagaac ccttggaagt ctcagaatgg
     4501 aataaaaata gcagtaagga tgtgaaaatc cccgacacac tgcaagatca attaaatgaa
     4561 cagcaaaaaa ggcaaccgct ctctgtttct gaccgtcatg tctacaagta tcgctgtaac
     4621 cattgtagct tggctttcaa aactatgcag aagcttcaga tacattccca gtatcatgca
     4681 attcgggctg cgacaatgtg taacctctgc cagcgcagtt tccgtacatt ccaggcttta
     4741 aaaaaacact tggaagcagg ccaccctgaa ctgagtgaag ctgaacttca acagctatat
     4801 gcctccttgc ccgtgaatgg agaactgtgg gcagagagcg aaactatgtc ccaggatgac
     4861 catggcctag agcaggaaat ggagagagag tatgaggtgg accacgaagg gaaagcaagt
     4921 cctgtaggaa gtgatagtag ctctattcca gatgacatgg gctctgaacc aaagcggacc
     4981 ttacctttta gaaaagggcc caattttacg atggaaaaat tccttgatcc atctcgtcca
     5041 tataaatgta cagtgtgtaa agagtcattc acccaaaaga acattctctt ggtccactat
     5101 aattcagttt ctcacttgca taagctgaaa aaagttttgc aggaagcctc cagtcctgtc
     5161 ccacaagaaa ccaacagcaa cacagataac aaaccctaca agtgcagcat ctgcaatgtt
     5221 gcatacagcc aaagctcaac attggaaatc cacatgaggt ctgtgctcca ccagacaaag
     5281 gctagggctg caaagctgga gcccagtggt catgtggctg gtgggcacag cattgcagca
     5341 aatgtcaaca gccctggcca ggggatgtta gattccatga gtttagcagc tgtaaacagc
     5401 aaagataccc atttagatgc caaagaatta aataaaaagc aaactcctga tttaatctct
     5461 gctcaacctg cacatcaccc accacagtca ccagcacaaa ttcagatgca actacagcac
     5521 gaattacaac agcaagccgc attctttcag cctcagtttc taaacccagc ctttttgcct
     5581 cattttccta tgaccccaga agcactgctg cagtttcagc agcctcagtt tctctttcca
     5641 ttttatatac ctgggacgga gttcagcttg gggccagatt tgggcttgcc aggctctgcc
     5701 acatttggga tgcctggcat gacaggaatg gctggctcct tgcttgaaga cctaaagcag
     5761 cagattcaaa cccaacatca cgttggtcaa actcaactcc agatactaca gcaacaagca
     5821 caacaatacc aagccacaca gccccagctg cagcctcaaa aacaacagca gcagccacca
     5881 cctccacagc agcagcagca acagcaggca agcaaattat tgaaacaaga gcaaagtaac
     5941 atagtgagtg cagactgcca aatcatgaag gatgtgccat cttataagga ggcagaagat
     6001 atttctgaaa agccagaaaa accaaagcag gaatttataa gtgaaggtga aggactcaaa
     6061 gaaggcaaag acacaaagaa gcaaaaatcc ttggaaccat ccatcccacc accccgaata
     6121 gcttcagggg ccagaggaaa tgctgccaaa gcgttattgg aaaactttgg ttttgaactg
     6181 gtcattcagt ataacgaaaa caggcagaag gtacagaaga agggcaaaag tggtgaaggc
     6241 gaaaacactg acaaactaga atgtggaaca tgtggtaaat tgttttccaa tgttcttatt
     6301 ttaaagagtc accaagaaca tgtacatggg caattttttc catatgcagc gctagaaaaa
     6361 tttgctcgtc aatacaggga ggcctatgac aagctttatc caatttctcc atcttctcca
     6421 gaaacgccgc ccccgccacc tcctcctcct cccttgcctc cggctcctcc acagccttct
     6481 tctatgggtc ctgtaaagat ccccaacacg gtttctactc ctctgcaagc tccaccaccc
     6541 actcctcccc caccaccacc acctcctcct cctcctcctc ctcccccccc acctcctcca
     6601 ccttctgctc ctccacaggt ccaactgccg gtttctctgg acctgccgct ctttccttcc
     6661 attatgatgc aacctgtgca acaccctgcg cttcctcccc agcttgccct gcagctgcca
     6721 cagatggacg cactctctgc agacctcacc caactttgcc agcagcagct cggattagat
     6781 cccaacttct taagacattc tcagttcaaa cgcccacgga caagaattac agatgatcag
     6841 ctaaaaatcc tgagggctta ttttgacatt aataattctc caagtgaaga acagatccag
     6901 gaaatggcag agaaatctgg cctctcccaa aaagttatca aacactggtt tagaaatacg
     6961 ctttttaagg aacgacagag aaataaagat tcaccataca acttcagtaa ccctcctata
     7021 acggttttag aagatatcag aattgatcca cagcccacct ctttagaaca ttacaaatct
     7081 gatgcatcat tcagtaaaag gtcttctaga acgagattta ctgactacca gcttagggtt
     7141 ctgcaagact tttttgacac aaacgcttac ccaaaagatg atgaaataga acaactctcc
     7201 actgttctca atctgcctac ccgggttatt gttgtatggt tccagaatgc tcgtcagaaa
     7261 gcacgaaaga gttatgagaa tcaagcagaa acaaaagata atgaaaaaag agaactcact
     7321 aatgaacggt acattcgaac aagcaacatg cagtaccagt gtaaaaagtg caatgtggtt
     7381 ttccccagga tctttgactt gattacgcat cagaaaaagc agtgttacaa ggatgaagat
     7441 gatgatgccc aagatgaaag ccaaacagaa gactccatgg atgccactga tcaagtggta
     7501 tacaagcatt gcacagtgtc tggccaaacg gatgcagcta aaaacgctgc tgcccctgca
     7561 gcaagttctg gctctgggac cagcaccccc ctgattccat cacccaaacc agaacctgag
     7621 aagacttctc caaaacctga atatcccgca gaaaagccaa agcagagtga cccctctccc
     7681 ccttctcaag gcaccaaacc agccctgcca ttagcatcga cttcctcgga cccaccacag
     7741 gcatccacag cccagccaca gccacagcca cagccaccaa aacaacccca acttatcgga
     7801 agacctccct cggcctctca aacaccggtc ccttccagtc cactgcaaat ttccatgacg
     7861 tctctccaga acagtctacc tccacagtta ctacaatacc aatgtgatca gtgtacagtt
     7921 gccttcccaa ctctggaact ctggcaggaa caccagcaca tgcacttcct tgctgctcaa
     7981 aaccaattcc ttcactctcc gttcttggaa aggcccatgg acatgcccta catgatattt
     8041 gaccccaaca atccgctgat gactggacaa ctgctgggca gttccctcac tcaaatgccc
     8101 cctcaggcca gttcctccca caccacagcc cccacaacgg ttgctgcttc cctaaaaagg
     8161 aaactagacg ataaagaaga taataattgc agtgaaaaag aaggagggaa tagcggtgaa
     8221 gaccaacacc gagataaacg cttgagaacc acgatcaccc cggaacagct ggaaatactc
     8281 tatgaaaaat acttgctgga ttccaatcct accagaaaaa tgcttgatca tattgcccgc
     8341 gaagtcgggc tgaaaaaaag ggtcgtgcaa gtctggttcc agaatacacg agcgcgggag
     8401 aggaaaggcc agttccgggc ggtgggtcca gcacagtctc ataaacggtg tccgttttgc
     8461 cgagccctgt ttaaagcaaa gtcggcctta gaaagccaca ttcgctctcg gcactggaat
     8521 gaaggaaagc aggcaggtta cagcttgcca ccaagccctt taatatccac cgaagatggg
     8581 ggagaaagcc cacagaaata catctatttt gattacccat ctttgccatt aactaaaatt
     8641 gatctatcaa gtgagaatga attggcttct acagtgtcaa cacctgttag taaaacagca
     8701 gagctgtcac cgaagaatct tttaagccct tcttctttta aagcagagtg ttctgaggat
     8761 gtagagaatt taaatgcccc tcctgctgag gctgggtatg atcaaaataa aaccgatttt
     8821 gatgagactt catcgattaa tacggcaatc agtgacgcca ccaccggaga cgagggaaac
     8881 actgaaatgg aaagcaccac aggaagttcc ggagatgtga aaccggcttt gtctcccaaa
     8941 gagccaaaaa ctctggatac tctgccaaaa cctgcaacca cacctaccac ggaggtctgc
     9001 gatgacaaat ttctcttttc tctcacaagc ccatccatcc atttcaatga caaagatggc
     9061 gaccacgacc aaagctttta catcacagat gacccggatg acaacgccga ccgcagcgaa
     9121 acgtccagca tagcggaccc gagctcccca aatccattcg gatccagcaa tccctttaaa
     9181 tccaaaagta atgatcggcc gggtcacaag cgttttcgaa cgcaaatgag caatcttcaa
     9241 ctcaaggttc tcaaggcttg ctttagtgac taccgaactc caaccatgca agaatgtgaa
     9301 atgttaggga atgagattgg tctgcccaaa cgcgtagtcc aggtgtggtt ccaaaatgca
     9361 agggcaaagg aaaagaaatt taaaattaac atagggaagc ctttcatgat caatcaaggc
     9421 ggaacggaag gcaccaaacc agagtgtacc ctctgcgggg tgaagtactc tgcccgcttg
     9481 tccatcagag atcacatttt ctccaaacag cacatttcaa aagtgaggga gaccgttggc
     9541 agtcagctcg atcgggagaa agattacttg gctccgacca cggttcggca gctgatggca
     9601 cagcaagaac ttgatcgtat aaagaaagct tcagacgtgc tgggcttgac ggtacagcag
     9661 ccaggcatga tggacagcag ttctctccac ggcatcagcc tgccaacagc ctaccccgga
     9721 ctccccggcc ttcctccagt ccttctcccc ggaatgaacg gtccatcctc cttgccggga
     9781 tttccacaaa attcaaacac tttaacacct cccggtgcag gcatgcttgg gtttcctact
     9841 tcagctactt cgtctcctgc cctgtctctc agcagtgccc ccaccaaacc tttgctgcag
     9901 actccaccac ctccaccacc tcctcctcct cctcctcctt catcctctct gtcaggacag
     9961 cagaccgagc aacagaacaa agaatctgag aaaaagcaaa ctaagccaaa caaggtgaaa
    10021 aaaatcaaag aggaggaatt agaggccacc aaacccgaaa aacaccccaa aaaagaggaa
    10081 aaaatctcat ctgctctttc agtgttgggc aaagttgtag gtgaaacaca tgtcgatcct
    10141 attcagttgc aggcattaca gaatgcaatt gctggtgacc cagcttcctt tataggcgga
    10201 cagttcttgc catactttat ccctgggttt gcttcttatt ttacacctca gctccctgga
    10261 acagtgcagg ggggatactt cccacctgtc tgtggcatgg agagcctctt tccttatggc
    10321 cctacaatgc cccagacact ggcaggtctg tccccaggtg cactgttgca gcagtaccaa
    10381 cagtatcagc agaacctgca ggagtccctg caaaagcagc aaaagcaaca gcaagaacag
    10441 cagcagaaac cagttcaggc aaagacatcc aaagtagaaa gtgaccagcc gcaaaactcc
    10501 aacgatgctt cagaaacaaa ggaagacaaa agtactgcta cagaaagcac aaaagaagaa
    10561 ccccagttag aatccaaaag tgcagacttt tcagacactt acgttgttcc attcgtcaag
    10621 tatgagttta tatgcagaaa gtgccagatg atgtttactg atgaagacgc cgcagtaaat
    10681 catcaaaagt ccttctgtta tttcggtcag cctttgattg acccacaaga gacagtgctt
    10741 cgtgtcccag tcagcaaata tcagtgtctt gcctgtgatg tggctatcag tgggaatgaa
    10801 gcacttagcc aacacctcca gtcaagcttg cacaaagaga aaacaatcaa acaagcaatg
    10861 agaaatgcca aagagcatgt tagattatta cctcactcag tctgctcccc taatcctaac
    10921 accacatcta cctcgcagtc tgcagcttct tctaataaca cctatcctca tctttcttgc
    10981 ttctccatga agtcctggcc taatatcctt ttccaagcgt ctgccaggag agctgcttct
    11041 cccccttctt ctcctccttc cctttccttg ccttcaacgg ttacctcaag tttgtgcagc
    11101 acctcagggg ttcaaacctc actacccaca gaaagttgtt cagatgagtc tgacagtgag
    11161 ctgagccaga agctagaaga cttagataat tctttggaag tgaaggctaa gcctgcttct
    11221 ggcctagatg gtaatttcaa tagcatccga atggatatgt tcagtgtgta ggagtgaaga
    11281 caggatcccg tgcttaaaaa aataaaaaat aaaaaaataa aaaaaaaata agactttaac
    11341 tgcagttcca aagcttctct aacccaaaaa ttacagtacc aaatgattga ctcaggattg
    11401 tttttcccat attgatatgc tggcaatata ggatggtatg taatggacag aactgatgca
    11461 gatggttgaa tgcgcttgta ctatatgcta aaatatggaa aaggaaaaaa aaatctcaca
    11521 agttcttttg gaacttgttt caagccaaaa actctcaaga aagcaaattg cacctcagct
    11581 ggattgattt ccaaatgcta gcatgtactg tatgggagga tgatccagat gtttcaaaga
    11641 gaatttctct tagtttagtt aggtgtaatt cagtagcttt aaattctcag gtcagaacat
    11701 aacatttctc atttgttaaa agcagcaaga agcctggtaa aactgtgact tttccccaaa
    11761 cgtcaatctt tattagaaag cattttctag gtgtgtttag tgtacaaaga gactttataa
    11821 cccttactgg acaacacaca gatccttgag ctcacgctgc aggatagtac agttttaccg
    11881 cagagggaat ctggaacagt ggaatcatgt gtctgccctg tgtattgcag tttgtattgc
    11941 cacaagctat atttatacca gtgtcaccct tttcttgtag aatatactaa taatctgtgc
    12001 caactctacc ttctcacttt tacctctgac gtcattcttt ttttctgaaa gaggtaataa
    12061 ttctagtttt gatagactct gaggattatg tgaacaggac atttttcatt tgtgaattta
    12121 atgctatact gtcaaggtac ttgcttgtgt ctgaactcta gtgcacttat gattttgtag
    12181 accatgtgaa atttaataag ataccttttt tttcctttct ttgtgtgtag tgcagcaaca
    12241 gtttggtctg catttgttag aagtttaact cctaacaacc caaagaccta tttaacaatt
    12301 ggtgcataaa tgaaagtagt actgtatact tgaaactgtt taagtacaag ttgaacaaaa
    12361 attatgaaaa ggtatatttg cttctcggga aagcaaagaa gctgctttaa aaaataaaaa
    12421 ggggactaaa aatttgtttt gtataaagag gttagccctg cgcacgtagg actgaattca
    12481 gtgatatccc tatacactgc catttagtgg ataggttatt gtacttccat tcatactctg
    12541 ggcacttgtg ttgtattgtt ctgttacata ctttttttaa cctgttttgt tttatcatat
    12601 atgcattaaa agtattatct ttatcaacat ttgctgctac tgtgttaaca tttttgtttt
    12661 gcttgccatg aatttcaact tccaccaccc agtgaattga tttataaatt gctatgcttt
    12721 gctgtttttc tgttgctgtg gaacttaaag aatgtgaaag ctgtcaaagg gtattttacg
    12781 aatcactttt gtgtttgata tagtaaaaca atgtgattca ttccaaagta acagaaggtt
    12841 atttgtaaga aagttaaagg cttgtgaaca aagaaagcta agctgttgta catatttgta
    12901 gttggctgtg catggtacaa atttattaat atgaagaaat gcaaaatgta ttgcttttga
    12961 tatttctctt ccgagatgaa caagtagcat gtaatgcaac tgtttgacag tttaactcaa
    13021 gtcatgcttc aaactgtttt aatgatcaaa tcaagacaca tttcatttta cattttatta
    13081 ttgtacagtt tttgtttcgg atgatgatca cagcaatctt tattctatac attttatgtg
    13141 aactttttta atgtctttaa tttggatttt tttttttttt agtattttaa catttatttt
    13201 aatcctgaag acactttttt gattgtgttt cgtaagagac aacatggcct cctaaggtgc
    13261 aatcctgccg ctatagtgag ctaatgtcct gaatccaaag gcttcagaaa attgcttttg
    13321 cctttttcat gaatgttaag cagcagcatt gtgagatcga tctgtcctgg cagttaacac
    13381 gatgtgcaac agtgtgttag catggaacag aacgcttttc acaaaacaaa ggactgtttt
    13441 acaaatgatt attccgacag tgtgtcgaca taaactttta caactgcaca gcagccaaaa
    13501 aaagaaaaaa aaaagaaaaa aaactttaac tggatggacg ttgttagggt gagaaataaa
    13561 aggacagcct ccaaaggttg agaatgagaa ttgttttttc ctggatatca aagggattat
    13621 cacagcgcaa tcattgtcta cacaacatgt actctcaacg cctgggttac ataggaaatg
    13681 caccctgagg ttttaataaa agcccctatg gctataactt taaataaact aaaccaaaaa
    13741 tgttattgat gttttatata tagagagtag tctcattagt ttttgttact gtaatgtttg
    13801 aagtctcaaa tgcaccgtat tacggtaaat aacatggttt tgaaaacttt tttttatttt
    13861 gtcacagacc tgttgtcata gttgaaatga tgtttattgt agatggtatt tgaacttatt
    13921 cttctggaaa tagttcatca agtatgtttg ttgctcattg tgatacatta aaaactgtat
    13981 ctacata
//
