LOCUS       NM_001174096            5937 bp    mRNA    linear   PRI 22-MAR-2025
DEFINITION  Homo sapiens zinc finger E-box binding homeobox 1 (ZEB1),
            transcript variant 9, mRNA.
ACCESSION   NM_001174096
VERSION     NM_001174096.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Liu,W., Lin,R., Zhu,C., Chen,Y., Gao,Q. and Zhong,J.
  TITLE     CCT2 Regulates ZEB1-Induced EMT Gene Transcription to Promote the
            Metastasis and Tumorigenesis of Papillary Thyroid Carcinoma
  JOURNAL   Discov Med 36 (188), 1819-1830 (2024)
   PUBMED   39327245
  REMARK    GeneRIF: CCT2 Regulates ZEB1-Induced EMT Gene Transcription to
            Promote the Metastasis and Tumorigenesis of Papillary Thyroid
            Carcinoma.
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Schwab,A., Rao,Z., Zhang,J., Gollowitzer,A., Siebenkas,K.,
            Bindel,N., D'Avanzo,E., van Roey,R., Hajjaj,Y., Ozel,E.,
            Armstark,I., Bereuter,L., Su,F., Grander,J., Bonyadi Rad,E.,
            Groenewoud,A., Engel,F.B., Bell,G.W., Henry,W.S., Angeli,J.P.F.,
            Stemmler,M.P., Brabletz,S., Koeberle,A. and Brabletz,T.
  TITLE     Zeb1 mediates EMT/plasticity-associated ferroptosis sensitivity in
            cancer cells by regulating lipogenic enzyme expression and
            phospholipid composition
  JOURNAL   Nat Cell Biol 26 (9), 1470-1481 (2024)
   PUBMED   39009641
  REMARK    GeneRIF: Zeb1 mediates EMT/plasticity-associated ferroptosis
            sensitivity in cancer cells by regulating lipogenic enzyme
            expression and phospholipid composition.
REFERENCE   3  (bases 1 to 5937)
  AUTHORS   Zhang,F., Wang,Y., He,Y. and Dong,B.
  TITLE     Correlation of FBXO45 Expression Levels with Cancer Severity by
            ZEB1 Ubiquitin in Non-Small-Cell Lung Cancer
  JOURNAL   J Environ Pathol Toxicol Oncol 43 (4), 13-23 (2024)
   PUBMED   39016138
  REMARK    GeneRIF: Correlation of FBXO45 Expression Levels with Cancer
            Severity by ZEB1 Ubiquitin in Non-Small-Cell Lung Cancer.
REFERENCE   4  (bases 1 to 5937)
  AUTHORS   Mehta,J.S., Vithana,E.N., Tan,D.T., Yong,V.H., Yam,G.H., Law,R.W.,
            Chong,W.G., Pang,C.P. and Aung,T.
  TITLE     Analysis of the posterior polymorphous corneal dystrophy 3 gene,
            TCF8, in late-onset Fuchs endothelial corneal dystrophy
  JOURNAL   Invest Ophthalmol Vis Sci 49 (1), 184-188 (2008)
   PUBMED   18172091
  REMARK    GeneRIF: The identification of a novel missense mutation in only
            one of the patients implied that TCF8 does not play a significant
            role in the pathogenesis of FECD in this Chinese population.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   5  (bases 1 to 5937)
  AUTHORS   Manavella,P.A., Roqueiro,G., Darling,D.S. and Cabanillas,A.M.
  TITLE     The ZFHX1A gene is differentially autoregulated by its isoforms
  JOURNAL   Biochem Biophys Res Commun 360 (3), 621-626 (2007)
   PUBMED   17610840
  REMARK    GeneRIF: The Zfhx1a-1 gene is autoregulated in part by negative
            feedback on its own promoter which is, in turn, modified by the
            availability of the negative dominant isoform Zfhx1a-2.
REFERENCE   6  (bases 1 to 5937)
  AUTHORS   Liskova,P., Tuft,S.J., Gwilliam,R., Ebenezer,N.D., Jirsova,K.,
            Prescott,Q., Martincova,R., Pretorius,M., Sinclair,N., Boase,D.L.,
            Jeffrey,M.J., Deloukas,P., Hardcastle,A.J., Filipec,M. and
            Bhattacharya,S.S.
  TITLE     Novel mutations in the ZEB1 gene identified in Czech and British
            patients with posterior polymorphous corneal dystrophy
  JOURNAL   Hum Mutat 28 (6), 638 (2007)
   PUBMED   17437275
  REMARK    GeneRIF: Four novel pathogenic mutations were identified in four
            families; two deletions, one nonsense, and one duplication within
            exon 7 in the ZEB1 gene at 10p11.2. Czech patients were genotyped
            for a founder haplotype and lack of 20p11.2 locus segregation.
REFERENCE   7  (bases 1 to 5937)
  AUTHORS   Franklin,A.J., Jetton,T.L., Shelton,K.D. and Magnuson,M.A.
  TITLE     BZP, a novel serum-responsive zinc finger protein that inhibits
            gene transcription
  JOURNAL   Mol Cell Biol 14 (10), 6773-6788 (1994)
   PUBMED   7935395
REFERENCE   8  (bases 1 to 5937)
  AUTHORS   Watanabe,Y., Kawakami,K., Hirayama,Y. and Nagano,K.
  TITLE     Transcription factors positively and negatively regulating the
            Na,K-ATPase alpha 1 subunit gene
  JOURNAL   J Biochem 114 (6), 849-855 (1993)
   PUBMED   8138542
REFERENCE   9  (bases 1 to 5937)
  AUTHORS   Williams,T.M., Montoya,G., Wu,Y., Eddy,R.L., Byers,M.G. and
            Shows,T.B.
  TITLE     The TCF8 gene encoding a zinc finger protein (Nil-2-a) resides on
            human chromosome 10p11.2
  JOURNAL   Genomics 14 (1), 194-196 (1992)
   PUBMED   1427828
REFERENCE   10 (bases 1 to 5937)
  AUTHORS   Williams,T.M., Moolten,D., Burlein,J., Romano,J., Bhaerman,R.,
            Godillot,A., Mellon,M., Rauscher,F.J. 3rd and Kant,J.A.
  TITLE     Identification of a zinc finger protein that inhibits IL-2 gene
            expression
  JOURNAL   Science 254 (5039), 1791-1794 (1991)
   PUBMED   1840704
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL117340.3, AL158080.21,
            AL355148.9 and AL161935.10.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 28, 2020 this sequence version replaced NM_001174096.1.
            
            Summary: This gene encodes a zinc finger transcription factor. The
            encoded protein likely plays a role in transcriptional repression
            of interleukin 2. Mutations in this gene have been associated with
            posterior polymorphous corneal dystrophy-3 and late-onset Fuchs
            endothelial corneal dystrophy. Alternatively spliced transcript
            variants encoding different isoforms have been described.[provided
            by RefSeq, Mar 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.18690.1,
                                           SRR14038193.2509225.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000424869.6/ ENSP00000415961.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                AL117340.3         73817-73893
            78-278              AL158080.21        28221-28421
            279-341             AL158080.21        62960-63022
            342-503             AL355148.9         5496-5657
            504-706             AL355148.9         13821-14023
            707-812             AL355148.9         17751-17856
            813-1181            AL355148.9         23274-23642
            1182-2623           AL161935.10        101-1542
            2624-2804           AL161935.10        3539-3719
            2805-5937           AL161935.10        6278-9410
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p11.22"
     gene            1..5937
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /note="zinc finger E-box binding homeobox 1"
                     /db_xref="GeneID:6935"
                     /db_xref="HGNC:HGNC:11642"
                     /db_xref="MIM:189909"
     exon            1..77
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..3397
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /note="isoform f is encoded by transcript variant 9; zinc
                     finger homeodomain enhancer-binding protein; posterior
                     polymorphous corneal dystrophy 3; delta-crystallin
                     enhancer binding factor 1; transcription factor 8
                     (represses interleukin 2 expression); negative regulator
                     of IL2"
                     /codon_start=1
                     /product="zinc finger E-box-binding homeobox 1 isoform f"
                     /protein_id="NP_001167567.1"
                     /db_xref="CCDS:CCDS53505.1"
                     /db_xref="GeneID:6935"
                     /db_xref="HGNC:HGNC:11642"
                     /db_xref="MIM:189909"
                     /translation="MADGPRCKRRKQANPRRNNVTNYNTVVETNSDSDDEDKLHIVEE
                     ESVTDAADCEGVPEDDLPTDQTVLPGRSSEREGNAKNCWEDDTGKEGQEILGPEAQAD
                     EAGCTVKDDECESDAENEQNHDPNVEEFLQQQDTAVIFPEAPEEDQRQGTPEASGHDE
                     NGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLER
                     HMTSHKSGRDQRHVTQSGCNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKK
                     RFSHSGSYSSHISSKKCISLIPVNGRPRTGLKTSQCSSPSLSASPGSPTRPQIRQKIE
                     NKPLQEQLSVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFTGGGPLQATSSPQGMV
                     QAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNQANLASKEQETINASPI
                     QQGGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKENPVATNSCKSEK
                     LPEDLTVKSEKDKSFEGGVNDSTCLLCDDCPGDINALPELKHYDLKQPTQPPPLPAAE
                     AEKPESSVSSATGDGNLSPSQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLD
                     VVKKWFEKMQAGQISVQSSEPSSPEPGKVNIPAKNNDQPQSANANEPQDSTVNLQSPL
                     KMTNSPVLPVGSTTNGSRSSTPSPSPLNLSSSRNTQGYLYTAEGAQEEPQVEPLDLSL
                     PKQQGELLERSTITSVYQNSVYSVQEEPLNLSCAKKEPQKDSCVTDSEPVVNVIPPSA
                     NPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQE
                     PPLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIF
                     QKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSH
                     SGSYSQHMNHRYSYCKREAEERDSTEQEEAGPEILSNEHVGARASPSQGDSDERESLT
                     REEDEDSEKEEEEEDKEMEELQEEKECEKPQGDEEEEEEEEEVEEEEVEEAENEGEEA
                     KTEGLMKDDRAESQASSLGQKVGESSEQVSEEKTNEA"
     exon            78..278
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /inference="alignment:Splign:2.1.0"
     exon            279..341
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /inference="alignment:Splign:2.1.0"
     exon            342..503
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /inference="alignment:Splign:2.1.0"
     exon            504..706
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /inference="alignment:Splign:2.1.0"
     exon            707..812
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /inference="alignment:Splign:2.1.0"
     exon            813..2623
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /inference="alignment:Splign:2.1.0"
     exon            2624..2804
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..5937
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5311..5316
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /note="hexamer: AATAAA"
     polyA_site      5335
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /note="major polyA site"
     regulatory      5891..5896
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /note="hexamer: ATTAAA"
     regulatory      5916..5921
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
                     /note="hexamer: AATAAA"
     polyA_site      5921
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
     polyA_site      5937
                     /gene="ZEB1"
                     /gene_synonym="AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;
                     TCF8; ZFHEP; ZFHX1A"
ORIGIN      
        1 agacacaagc gagaggatca tggcggatgg ccccaggtgt aagcgcagaa agcaggcgaa
       61 cccgcggcgc aataacgtta caaattataa tactgtggta gaaacaaatt cagattcaga
      121 tgatgaagac aaactgcata ttgtggaaga agaaagtgtt acagatgcag ctgactgtga
      181 aggtgtacca gaggatgacc tgccaacaga ccagacagtg ttaccaggga ggagcagtga
      241 aagagaaggg aatgctaaga actgctggga ggatgacaca ggaaaggaag ggcaagaaat
      301 cctggggcct gaagctcagg cagatgaagc aggatgtaca gtaaaagatg atgaatgcga
      361 gtcagatgca gaaaatgagc aaaaccatga tcctaatgtt gaagagtttc tacaacaaca
      421 agacactgct gtcatttttc ctgaggcacc tgaagaggac cagaggcagg gcacaccaga
      481 agccagtggt catgatgaaa atggaacacc agatgcattt tcacaattac tcacctgtcc
      541 atattgtgat agaggctata aacgctttac ctctctgaaa gaacacatta aatatcgtca
      601 tgaaaagaat gaagataact ttagttgctc cctgtgcagt tacacctttg catacagaac
      661 ccaacttgaa cgtcacatga catcacataa atcaggaaga gatcaaagac atgtgacgca
      721 gtctgggtgt aatcgtaaat tcaaatgcac tgagtgtgga aaagctttca aatacaaaca
      781 tcacctaaaa gagcacttaa gaattcacag tggagagaag ccatatgaat gcccaaactg
      841 caagaaacgc ttttcccatt ctggctccta tagctcacac ataagcagta agaaatgtat
      901 cagcttgata cctgtgaatg ggcgaccaag aacaggactc aagacatctc agtgttcttc
      961 accgtctctt tcagcatcac caggcagtcc cacacgacca cagatacggc aaaagataga
     1021 gaataaaccc cttcaagaac aactttctgt taaccaaatt aaaactgaac ctgtggatta
     1081 tgaattcaaa cccatagtgg ttgcttcagg aatcaactgt tcaacccctt tacaaaatgg
     1141 ggttttcact ggtggtggcc cattacaggc aaccagttct cctcagggca tggtgcaagc
     1201 tgttgttctg ccaacagttg gtttggtgtc tcccataagt atcaatttaa gtgatattca
     1261 gaatgtactt aaagtggcgg tagatggtaa tgtaataagg caagtgttgg agaataatca
     1321 agccaatctt gcatccaaag aacaagaaac aatcaatgct tcacccatac aacaaggtgg
     1381 ccattctgtt atttcagcca tcagtcttcc tttggttgat caagatggaa caaccaaaat
     1441 tatcatcaac tacagtcttg agcagcctag ccaacttcaa gttgttcctc aaaatttaaa
     1501 aaaagaaaat ccagtcgcta caaacagttg taaaagtgaa aagttaccag aagatcttac
     1561 tgttaagtct gagaaggaca aaagctttga agggggggtg aatgatagca cttgtcttct
     1621 gtgtgatgat tgtccaggag atattaatgc acttccagaa ttaaagcact atgacctaaa
     1681 gcagcctact cagcctcctc cactccctgc agcagaagct gagaagcctg agtcctctgt
     1741 ttcatcagct actggagatg gcaatttgtc tcctagtcag ccacctttaa agaacctctt
     1801 gtctctccta aaagcatatt atgctttgaa tgcacaacca agtgcagaag agctctcaaa
     1861 aattgctgat tcagtaaacc taccactgga tgtagtaaaa aagtggtttg aaaagatgca
     1921 agctggacag atttcagtgc agtcttctga accatcttct cctgaaccag gcaaagtaaa
     1981 tatccctgcc aagaacaatg atcagcctca atctgcaaat gcaaatgaac cccaggacag
     2041 cacagtaaat ctacaaagtc ctttgaagat gactaactcc ccagttttac cagtgggatc
     2101 aaccaccaat ggttccagaa gtagtacacc atccccatca cctctaaacc tttcctcatc
     2161 cagaaataca cagggttact tgtacacagc tgagggtgca caagaagagc cacaagtaga
     2221 acctcttgat ctttcactac caaagcaaca gggagaatta ttagaaaggt caactatcac
     2281 tagtgtttac cagaacagtg tttattctgt ccaggaagaa cccttgaact tgtcttgcgc
     2341 aaaaaaggag ccacaaaagg acagttgtgt tacagactca gaaccagttg taaatgtaat
     2401 cccaccaagt gccaacccca taaatatcgc tatacctaca gtcactgccc agttacccac
     2461 aatcgtggcc attgctgacc agaacagtgt tccatgctta agagcgctag ctgccaataa
     2521 gcaaacgatt ctgattcccc aggtggcata cacctactca actacggtca gccctgcagt
     2581 ccaagaacca cccttgaaag tgatccagcc aaatggaaat caggatgaaa gacaagatac
     2641 tagctcagaa ggagtatcaa atgtagagga tcagaatgac tctgattcta caccgcccaa
     2701 aaagaaaatg cggaagacag aaaatggaat gtatgcttgt gatttgtgtg acaagatatt
     2761 ccaaaagagt agttcattat tgagacataa atatgaacac acaggtaaaa gacctcatga
     2821 gtgtggaatc tgtaaaaagg catttaaaca caaacatcat ttgattgaac acatgcgatt
     2881 acattctgga gaaaagccct atcaatgtga caaatgtgga aagcgcttct cacactctgg
     2941 gtcttattct caacacatga atcatcgcta ctcctactgt aagagagaag cggaagaacg
     3001 tgacagcaca gagcaggaag aggcagggcc tgaaatcctc tcgaatgagc acgtgggtgc
     3061 cagggcgtct ccctcacagg gcgactcgga cgagagagag agtttgacaa gggaagagga
     3121 tgaagacagt gaaaaagagg aagaggagga ggataaagag atggaagaat tgcaggaaga
     3181 aaaagaatgt gaaaaaccac aaggggatga ggaagaggag gaggaggagg aagaagtgga
     3241 agaagaagag gtagaagagg cagagaatga gggagaagaa gcaaaaactg aaggtctgat
     3301 gaaggatgac agggctgaaa gtcaagcaag cagcttagga caaaaagtag gcgagagtag
     3361 tgagcaagtg tctgaagaaa agacaaatga agcctaatcg tttttctaga aggaaaataa
     3421 attctaattg ataatgaatt tcgttcaata ttatccttgc ttttcatgga aacacagtaa
     3481 cctgtatgct gtgattcctg ttcactactg tgtaaagtaa aaactaaaaa aatacaaaat
     3541 acaaaacaca cacacacaca cacacacaca cacacacaca cacacaaaat aaatccgggt
     3601 gtgcctgaac ctcagaccta gtaatttttc atgcagtttt caaagttagg aacaagtttg
     3661 taacatgcag cagattagaa aaccttaatg actcagagag caacaataca agaggttaaa
     3721 ggaagctgat taattagata tgcatctggc attgttttat cttatcagta ttatcactct
     3781 tatgttggtt tattcttaag ctgtacaatt gggagaaatt ttataatttt ttattggtaa
     3841 acatatgcta aatccgcttc agtattttat tatgtttttt aaaatgtgag aacttctgca
     3901 ctacaaaatt cccttcacag agaagtataa tgtagttcca acccgtgcta actacctttt
     3961 ataaattcag tctagaaggt agtaatttct aatatttaga tgtcttagta gagcgtatta
     4021 tcatttaaag tgtattgtta gccttaagaa agcagctgat agaagaactg aagtttctta
     4081 ctcacgtggt ttaaaatgga gttcaaaaga ttgccattga gttctgattg cagggactaa
     4141 caatgttaat ctgataagga cagcaaaatc atcagaatca gtgtttgtga ttgtgtttga
     4201 atatgtggta acatatgaag gatatgacat gaagctttgt atctcctttg gccttaagca
     4261 agacctgtgt gctgtaagtg ccatttctca gtattttcaa ggctctaacc cgccttcatc
     4321 caatgtgtgg cctacaataa ctagcatttg ttgatttgtc tcttgtatca aaattcccaa
     4381 ataaaactta aaaccactga ctctgtcaga gaaactgaaa cactgggaca tttcatcctt
     4441 caattcctcg gtattgattt tatgttgatt gattttcaga atttctctac agaaacgaaa
     4501 gggaaatttt ctaatctgct ttatccatgt acttgcattt cagacatgga catgctattg
     4561 ttatttggct cataactgtt tccaaatgtt agttattatg gacccaattt attaacaaca
     4621 ttagctgatt tttacctatc agtattattt tatttctttt agtttataga tctgtgcaac
     4681 atttttgtac tgtatgtctt caaacctggc agtattaata cccttcttac tgacatatgt
     4741 acttttagtt ttagaaaact tttatattta tgtgtcttat ttttatattt ctttatttat
     4801 tacacagtgt agtgtataat actgtagttt gtattaatac aataatatat tttagtatga
     4861 aaatttggaa agttgataag atttaaagta gagatgcaat tggttctcct gcattgagat
     4921 ttgatttaac agtgttatgt taacatttat acttgccttg gactgtagaa cagaacttaa
     4981 atgggaatgt attagtttta caactacaat caagtcattt tacctttacc cagtttttaa
     5041 tataaaactt aaattttgaa attcactgtg tgactaatag catgatgctc tgcagtttta
     5101 ttaagaaatc agcctaacca tacaactctc atttccttag taagccaaat taggattaac
     5161 ttctataaac agtgttggga acaatgttta acattttgtg ccaatttgtt cctgtattca
     5221 tgtatgtaag ttacagatct gactcttcat ttttaagttc cttgttacat catggtcatt
     5281 ttctagtttt ttaccagact cccatctcac aataaaatgc atcaacaagc ctgaactgct
     5341 gtcattcttt tcatcattat cagtattttc tttggaaaac tgtgaaatgg ggtacattgt
     5401 catcctgcat ttgattcatc ttgagctgaa tttgggtaac actaaatgtt ttagacattc
     5461 tccactaaat tatggatttt cttgtggcta aatgtttctg gagaggtcag agttgacaaa
     5521 acctcttcac aggttgctcc ttcttcctga aatccttaat cctccgcatt tcatgcttca
     5581 ggtcatttca gggaagcctg ggtttagatg cctttctgac tctcagctcc tgcacttctg
     5641 tcatcatacc tctgatacta ttatttatat tccttcccca ctaggaacag gaaccacatt
     5701 tgtcatagtc actctcacat tcctcactgc ctaacagggt gcctggcata agttgggaca
     5761 acagatattt gttgaataaa aatataattt gcatgtttat ggagctcagc tatgttctca
     5821 ctttttttgc ttctaattcc agaatatatg ttaaatgatc taataatttg attattttct
     5881 tataagtctt attaaacact agtcataata gacacaataa attatgcctt ctttttc
//
