LOCUS       NM_178566               9121 bp    mRNA    linear   PRI 06-MAR-2025
DEFINITION  Homo sapiens zDHHC palmitoyltransferase 21 (ZDHHC21), transcript
            variant 8, mRNA.
ACCESSION   NM_178566
VERSION     NM_178566.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Shao,X., Xu,A., Du,W., Xu,T., Huang,Y., Xia,Z., Wang,W., Cai,M.,
            Zhang,X., Zhang,J., Cao,J., Xu,X., Yang,B., He,Q. and Ying,M.
  TITLE     The palmitoyltransferase ZDHHC21 regulates oxidative
            phosphorylation to induce differentiation block and stemness in AML
  JOURNAL   Blood 142 (4), 365-381 (2023)
   PUBMED   37216691
  REMARK    GeneRIF: The palmitoyltransferase ZDHHC21 regulates oxidative
            phosphorylation to induce differentiation block and stemness in
            AML.
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 9121)
  AUTHORS   Beard,R.S. Jr., Yang,X., Meegan,J.E., Overstreet,J.W., Yang,C.G.,
            Elliott,J.A., Reynolds,J.J., Cha,B.J., Pivetti,C.D., Mitchell,D.A.,
            Wu,M.H., Deschenes,R.J. and Yuan,S.Y.
  TITLE     Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction
            in systemic inflammatory response syndrome
  JOURNAL   Nat Commun 7, 12823 (2016)
   PUBMED   27653213
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9121)
  AUTHORS   Marin,E.P., Jozsef,L., Di Lorenzo,A., Held,K.F., Luciano,A.K.,
            Melendez,J., Milstone,L.M., Velazquez,H. and Sessa,W.C.
  TITLE     The Protein Acyl Transferase ZDHHC21 Modulates alpha1 Adrenergic
            Receptor Function and Regulates Hemodynamics
  JOURNAL   Arterioscler Thromb Vasc Biol 36 (2), 370-379 (2016)
   PUBMED   26715683
REFERENCE   5  (bases 1 to 9121)
  AUTHORS   Ohno,Y., Kashio,A., Ogata,R., Ishitomi,A., Yamazaki,Y. and
            Kihara,A.
  TITLE     Analysis of substrate specificity of human DHHC protein
            acyltransferases using a yeast expression system
  JOURNAL   Mol Biol Cell 23 (23), 4543-4551 (2012)
   PUBMED   23034182
REFERENCE   6  (bases 1 to 9121)
  AUTHORS   Pedram,A., Razandi,M., Deschenes,R.J. and Levin,E.R.
  TITLE     DHHC-7 and -21 are palmitoylacyltransferases for sex steroid
            receptors
  JOURNAL   Mol Biol Cell 23 (1), 188-199 (2012)
   PUBMED   22031296
  REMARK    GeneRIF: the DHHC-7 and -21 proteins are novel targets to
            selectively inhibit membrane sex steroid receptor localization and
            function
REFERENCE   7  (bases 1 to 9121)
  AUTHORS   Eriksson,N., Tung,J.Y., Kiefer,A.K., Hinds,D.A., Francke,U.,
            Mountain,J.L. and Do,C.B.
  TITLE     Novel associations for hypothyroidism include known autoimmune risk
            loci
  JOURNAL   PLoS One 7 (4), e34442 (2012)
   PUBMED   22493691
REFERENCE   8  (bases 1 to 9121)
  AUTHORS   Mill,P., Lee,A.W., Fukata,Y., Tsutsumi,R., Fukata,M., Keighren,M.,
            Porter,R.M., McKie,L., Smyth,I. and Jackson,I.J.
  TITLE     Palmitoylation regulates epidermal homeostasis and hair follicle
            differentiation
  JOURNAL   PLoS Genet 5 (11), e1000748 (2009)
   PUBMED   19956733
REFERENCE   9  (bases 1 to 9121)
  AUTHORS   Fernandez-Hernando,C., Fukata,M., Bernatchez,P.N., Fukata,Y.,
            Lin,M.I., Bredt,D.S. and Sessa,W.C.
  TITLE     Identification of Golgi-localized acyl transferases that
            palmitoylate and regulate endothelial nitric oxide synthase
  JOURNAL   J Cell Biol 174 (3), 369-377 (2006)
   PUBMED   16864653
REFERENCE   10 (bases 1 to 9121)
  AUTHORS   Ohno,Y., Kihara,A., Sano,T. and Igarashi,Y.
  TITLE     Intracellular localization and tissue-specific distribution of
            human and yeast DHHC cysteine-rich domain-containing proteins
  JOURNAL   Biochim Biophys Acta 1761 (4), 474-483 (2006)
   PUBMED   16647879
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL390732.10 and AK292960.1.
            
            On Jun 2, 2019 this sequence version replaced NM_178566.5.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.3265586.1, BC042558.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380916.9/ ENSP00000370303.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-144               AL390732.10        96631-96774         c
            145-1333            AK292960.1         2-1190
            1334-9121           AL390732.10        14413-22200         c
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p22.3"
     gene            1..9121
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /note="zDHHC palmitoyltransferase 21"
                     /db_xref="GeneID:340481"
                     /db_xref="HGNC:HGNC:20750"
                     /db_xref="MIM:614605"
     exon            1..204
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     exon            205..253
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     exon            254..383
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     exon            384..582
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     CDS             429..1226
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /EC_number="2.3.1.225"
                     /note="isoform 1 is encoded by transcript variant 8; zinc
                     finger, DHHC domain containing 21; palmitoyltransferase
                     ZDHHC21; zinc finger DHHC-type containing 21; DHHC
                     domain-containing cysteine-rich protein 21; zinc finger
                     DHHC-type palmitoyltransferase 21"
                     /codon_start=1
                     /product="palmitoyltransferase ZDHHC21 isoform 1"
                     /protein_id="NP_848661.1"
                     /db_xref="CCDS:CCDS6475.1"
                     /db_xref="GeneID:340481"
                     /db_xref="HGNC:HGNC:20750"
                     /db_xref="MIM:614605"
                     /translation="MGLRIHFVVDPHGWCCMGLIVFVWLYNIVLIPKIVLFPHYEEGH
                     IPGILIIIFYGISIFCLVALVRASITDPGRLPENPKIPHGEREFWELCNKCNLMRPKR
                     SHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFYTELLTCYALMFSFCHYYYF
                     LPLKKRNLDLFVFRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTTSIEKMSNC
                     CEDISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRVPYHFANHV"
     exon            583..681
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     exon            682..793
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     exon            794..932
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1049
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1093
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..9121
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4377..4382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /note="hexamer: AGTAAA"
     polyA_site      4405
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /note="major polyA site"
     regulatory      4638..4643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /note="hexamer: AATAAA"
     polyA_site      4660
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /note="major polyA site"
     regulatory      9099..9104
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /note="hexamer: AATAAA"
     polyA_site      9121
                     /gene="ZDHHC21"
                     /gene_synonym="DHHC-21; DHHC21; DNZ1; HSPC097"
                     /note="major polyA site"
ORIGIN      
        1 agtcgtccct ccccacgcgg gcgcgcgggc atgcggacac ccactcggcc ggtccaggcc
       61 ctcaggctcc cggaagcgga aggggagagc ggcccggcct gggcggcggc gccggaggag
      121 gcggaggtgg cgcggcagga ggaggggaaa gagctgctgg cggtcgggag agcggcggca
      181 gcgagaggcg agccagcggc gacgaatgaa gaactttttc acttactgca ggattttcag
      241 cttcagcaag caggttctca tggagaactg ttattgaaga gagatccatt tggtgggata
      301 taattaaaaa aaatgaatca aaagaaaatc tctaaatgca gattccagaa aaattacagc
      361 acatctcagt gaatttgcaa gtggatcatc cttccttgtg ggttagcagg cagttataag
      421 attgcaaaat gggtctccgg attcactttg ttgttgaccc acatggttgg tgctgcatgg
      481 gtttgattgt ctttgtttgg ttatacaata ttgttttaat tcccaaaatt gtcctctttc
      541 ctcactatga agaaggacat attccaggca tattaataat aatattctat ggcatttcca
      601 tattctgtct ggttgcctta gtgagggcct ccataactga tccaggaaga ctccctgaga
      661 accccaagat cccacatgga gaaagggagt tctgggaatt atgtaacaag tgtaatttga
      721 tgagaccaaa gcgttcccat cactgtagcc gctgcggcca ctgtgtgagg agaatggatc
      781 atcactgtcc atggattaac aattgtgttg gtgaagataa tcattggctc tttctgcagt
      841 tgtgtttcta cactgaactt cttacttgct acgcactgat gttttctttc tgccactatt
      901 actattttct tccactaaaa aagcgtaatt tggacctctt tgtttttaga catgaattgg
      961 ccataatgag actagcagcc tttatgggca ttactatgtt agttggaata actggactct
     1021 tttacactca actaattggc atcatcacag atacaacatc tattgaaaag atgtcaaact
     1081 gttgtgaaga tatatcgagg ccccgaaagc catggcagca gaccttctca gaagtttttg
     1141 gcactcgttg gaagatcctg tggttcattc ctttcaggca gaggcaacca ctgcgagttc
     1201 cctaccactt tgccaatcat gtctaaacag atggatggtg ggcacagatg ggtcctccat
     1261 gctggcaatg cgttacaggt tttatgataa tagaactatg acagtcttca agtcaattaa
     1321 aatccaccca ccagtcttag gcatcataat gtgccccagg ctttatttta atagtgatct
     1381 tgatcctgtt gtgggactaa tacaagatct atatttaagt tttaaagcat gtttactttc
     1441 aaattagctt tccacaggga tttcttgaaa tgcttttgtt attaaactca aaaggcagtg
     1501 attaggatga aataattgta cataatttat tttagtactg acagtgttac agattctaat
     1561 ttatggtaaa tttcagatgt ttatttaaaa ttttcacttt taaacagtaa ccaaatctaa
     1621 atttaattat tcaggtttta caaaagttga tacaccttct tatagtatag gtaaattttc
     1681 tttttcaaat ccaatttaaa ataacctttc cttttaaatg tgctgcaacg tttttaaaaa
     1741 tgcagcagca taggagtgaa caacagcaac acaaaacggg cattggtttc ttaggagtgg
     1801 cttgcttacg ttttcttctt ttttcttcac caaaaccaac atgaaagtac cactgaagta
     1861 aaacaccaac tacctacctt actataaagg aaatgttaaa atttttttca caataatttt
     1921 ttcaattttc actattactg ttgtaattat tgattgtgat taaaatattt gctcccagga
     1981 gaactcctga ccagtgggca tgtattccta ttttatccta agattttaat gagcaaaaag
     2041 gggagagaat ttgcaataac ttcacaatta ccttttctag tgcagttata ttagaatgca
     2101 tatgttttta aaatgctagt ataactagat agtatataat gtacagtata aagaggaata
     2161 ttgtgctttt gaaaagagta tactttttac ttttatttat gtactagtag atgtaaaatt
     2221 tcacattgaa ggtttatata tattggccaa ctcatataga aattttattt ataggaagct
     2281 actactaaaa aaagtcacta actttgtgta cctataatcc ctaaattaaa attaaatttt
     2341 aattggctgg ctgtaatttg cattgagaga ccttttacta gtagctagtg ttaggaagtg
     2401 aacctaaaat aagaaaatat aatgatctgt gtttatcatt agccttgtac aaatgtaaat
     2461 tactaatagt gatgttcttt tgcaaggcat agaacatttg tatgaaaaga catttagtat
     2521 gctttgaaaa aaactcagct tttagttttt ttccattaga atactgcaaa acccatatat
     2581 ctttttaaaa aatttatgta ctcacagtct ttcccttgaa gagaagattg aaaaagtcta
     2641 ctgttcataa accatgctaa cattttcctt ttagctagtt ttgaaagtaa ggaacaatac
     2701 ctgggaaata attaaacaga aggttaccat tgtcagccag ttggctatac tgtggttagt
     2761 tctttcagaa attgtaaata tcttgtagca tattctgaaa taatagagta agttcttctc
     2821 agagatgtta atatcatgtt tttcatgttc taattagaat actttattac ttacaaactc
     2881 agaaatacga acagaaatac agcagacgaa catatttatt ggtactgaaa agagacgtag
     2941 taaattaaat agaagaaata tatttataaa gcttagtgaa acacaaaatt agaatgttca
     3001 tgtcaggcac aagggtttgg attttgtgca agctaatttg gccacatttg gcctggtgac
     3061 agaactgttc ataaggaagt aatatataga taaggtaggt agatatcagt tgaatgcctt
     3121 atattgtata cattcctttc aaataaagac cttgagaaaa cagcagagcc aagtgaagat
     3181 cacctaaaga actttgtggc taatttatac ttcatgtagt agcagtgggt actgggcagg
     3241 gtctctttcc cactctgatg atttgtgctc ttatttttcc tagatttacc tcatctaggg
     3301 catattcttt tccctcttcc tctttacctt tcctggtcct gatccctctg tactcagttc
     3361 ccctaaatta ttggactaca aactaatata ctagaaaagc atacacttat tttatttgaa
     3421 tgcagaaatg ctatctatca gtatatatac ataagaatgt atattacagt atatctatat
     3481 atacttaaca ctgtaacttt cagtattccc cagttagcgt acctaactct cctgtgggtt
     3541 ttgttaattc ttattagact actagagaaa aaccaactgg cagtttgcta agcatatcta
     3601 ctggtgttgt ttctgcgccc tcttttggct aattgatgta attatactgg ctctaaagat
     3661 ttactgcccc ataagtaaat agtatagcca cattctgaac atatcaaaag tacaaactta
     3721 ggaggagtgt atgtacaaaa atgtaaaatt ttatgaaaat gaacatgttt ttatgatgtt
     3781 atttctagtt cataagaatg tgatgactgc tttgcttcat ttatgtacgt tcccattata
     3841 ttcttgctgt caatcaatca caaatttata tcagattagg ataaactaag ccattttatg
     3901 tattttattt taaaccttat tttggcagag taattcctta gaattggaaa agctgttact
     3961 ttgaaattac caatttatta caaaacatag aaatgtattg tagctacaaa gacaaccaag
     4021 cattttctgt gttttaatga atatctaaaa aactacattt agtttatttt actcagtttt
     4081 gaaatgattt ttttactggc tctattgcct taaaataact aagagattaa tgattctttg
     4141 tataattttc cttttctttg ttcttttttt tacatttcgc agagttatat ctatagtttt
     4201 agtaacaatt tcttatgtat tctggataac tgaaaacaac taaaggtgtt gggcattaga
     4261 aaataattgt gagcagtaag attactgatg taatatgtat gttggactga agtatttctt
     4321 tataaacatt ctatttgatt ttaagcaaaa tgtatgttaa agcatgtttt tacatcagta
     4381 aagtcatttg tcgaccttct ggaaatgaaa ggtttttacc tagatactgt aagttacacc
     4441 tccttaacaa tcatatttgt cattgttgtt ttctgcaaac aaaaatgttt atgggcttca
     4501 tgtaggctta agattgtagg caaaaatgga ctgagttcag gacccttcaa gcagtaggca
     4561 ttcagttaca gagcagttgg tactttgtaa cccagactta cagtttaaaa atatcaagtt
     4621 agctgatgtt tcattataat aaaaatacta ttttgcttaa gagttgtatt acaaatattt
     4681 gtgcttaaca ttagaaatag ctgttttaaa ttgtagttaa catattaact ttttcagaaa
     4741 aaaagcatgg tttattttta ataatgaaat agagaacata atacgtaatg ttcagtataa
     4801 cagctgagtt aaaacatctg ccaggattaa catcagtgcg tttttgccaa tgcatagagg
     4861 catttttctc taagtatgat ggctaatgat aactattctt tgttacacat tcaagtcact
     4921 cccatacaag taactagtgg gtgatatgtt tcactccaaa gggtgtatta attctgaatg
     4981 ctaatcatga agacttaagt taggacaaca cttcaaacca ggaagtgtga actgatttag
     5041 attatagcta cacaattttc tgtgtgttag atcatggggt agtttgagtg ttttcacatg
     5101 tattgctata aaatcacaat gtaccaagct ctggtttaat atgccattaa tactaattaa
     5161 tagagctgct agtctctctc tggacttttc tctagtctct gcttctcttg cgcttcctgt
     5221 atcaatagat tacttagctc aagtttacca agtttaataa cctatagtta ctttattgaa
     5281 taagtttctg acatgacctc taatatacat agatatatct agaatttatt tgataaaatt
     5341 cacacataca aacattgagt agtttgctaa tagatacaaa tattagattt tcacacaact
     5401 ctactactgc tttaaaaata taagtattgg taagtgtagc aaataagcca tgtacattta
     5461 atgtgttcta cttgctagta ttaactgtat gattaatgaa tgtgttattt aatgcccaag
     5521 tttgttaact tttattctag aaatcatcct ctgaatttat tataaatcac agatttcatt
     5581 gtgctctata tttagtcatt ctcagttgaa ttctagaata ttccacagta cagtataaga
     5641 aagtattaga accaacttgc ttgaactatt ttgaaccagt gttgtaattc tatatgttaa
     5701 tcagtattta gcatttctgc tccttttgag atgatgatgg tttaacttca ggaaagtgac
     5761 ttttctgact tcttttatta tcattgtgca atttttagca acaaaactca ctatatcttt
     5821 gtcagttctt cagatgttac aatactgcct gctaatttga aacagaaata acaatggtat
     5881 tttaaaaagt cattgatatt actgatactt tccattatca ttatattttt tactattatt
     5941 atgttctttt tgccttgtcc accctagaga ataggtacaa atgagtagtg agtatccaca
     6001 aacttactaa gatggagaga aggctagatg gtgcatgaat aatcagcatc tcagcaacag
     6061 agttgactgg agccagtttc tgacatcaga gaatctcttt gctgtaatca ttttagcctt
     6121 atctcttatt tataccattt ttatgggaag agttgttagt gcatataagc atttagtttg
     6181 aattatttaa cttaaagaat ttatattgcg gtttctcttc agttggtcag atgcctttta
     6241 taaatatttt agttttaaat ataattgtgt agagtatgat catgtgaagt gttaaaaata
     6301 atgtggaaaa ctcagctatt taatgcagag gagaaatgaa taggttgacc aaagtttgaa
     6361 aaaactggca ccttttctgg aaatctctta ttgcaaagaa aagatactga gtgtatagaa
     6421 aattttgtca ccagaggaaa aataaacaat atataagggt ggctttcact ttaacatttg
     6481 ggatattttg gagttcttga tacctcgaag gtctccattt ctattggtat tttgaatatc
     6541 tgtaacatgt aatgtcgttc ttataacagt tcaacactga tttttttggt atcgatagct
     6601 ttatatataa acaaagatgc ggacaaaata gcttaacaga agttgtgggc ttttttgtgg
     6661 ctttgcctgc ctgtttgacc aaatacaggg tttaattgtt tgcaggctgt taactaaagg
     6721 gaaagcttac ctgcgtattg caagctatat attcttgcca gtatacattg gatgatattt
     6781 taacctactg gtgcctgaag ttcgtgtttt ataacctcaa caactataaa acatcctggt
     6841 gctattcact ttagcattga gtgacatttt taattcctgt accaaagagt taattttctt
     6901 tttggttgac tgagttttct tctcacatcg ttgtgtattt ataaagtaga atttaatgtt
     6961 actaagaaca gtttgattaa cagatcttat tttttgcatg ctgctctggc aatttaatgg
     7021 attttaaatg tttctcattt ggattttttt ttaggtaggc atgtatggat tttaattttg
     7081 tattttaata tagcttggct actttaaaag gtagtttgat aggctgaaat tacaaaagat
     7141 aattgaagca cttttcacaa gttgttttat ttctgcaaca atgaataaaa aggtaagaac
     7201 tctaatttat ataaatgaac tatatagctt attgggcact ttagtgaata ctgaaacaaa
     7261 atactaagat gaagagttaa ggttcgttct cattacaaat cgagttcagc gtgtgtgtgt
     7321 gtgtgtgtgt gtgtgtgtgt gtgtgtgtaa tctttagtgg ctttaaagaa taatattgcc
     7381 atttgtctac tttgcaaata aagataacag aacaccttta aaatatatgt atgtgtacat
     7441 atgtgtgtgt agatatatag agagagagat acggagagac agacagacag agagagagac
     7501 agagagagag atgctttccc aagaggaaat gcatatgtac cttgatttaa gcaaaactga
     7561 taacagaaaa ttgaacttaa tgaaagaaaa aagtctggat atggttactc actttacata
     7621 gagtaagatt tttaaaacta attttgctta gtagttaatt ttacaaaaat gaagagaaac
     7681 accctttaag tgtactcgta atttaaaaca cctttgaaat gtaattgtcc aaatgtaatg
     7741 ctgctatgta tattatttgg gttccagatt tttcccttta aaattctcaa aacactcagt
     7801 gtccttacaa ctgcagctaa aatggttaat cattctggca agaaggtgac agtgtgtata
     7861 ttggaaaggc agacattcac atttgaaaat attttgcatt attgttagct aaaatcaacc
     7921 tctccttgaa tgtagataca atatatggtt ttgcagtcaa ttctgaatgg aaaaaaagaa
     7981 tcaagtctga aatttatgtg aaatgtggct tcagatcatt tgaacgatat gtagaaatct
     8041 agatgaacat atcattagag gtgaaagcaa aatttgaaag cctaatgaga tgaagcaaca
     8101 ttatctctct ctaatctgcc aacaatattc cctagattgt gttgccactg tattgattct
     8161 gagtaatttg gcttgtttat gcgtgattta aatgcatgtg gctgaatgca gtaatttttc
     8221 aagttattga gtagtagaga attcaccact attcactgtc tcccagagat gaacatataa
     8281 aagaaaaaaa attctacttc atatatgtaa gaataaagca actaaatgat acaaaagtaa
     8341 aaacttccac cagaatcaga gatattccga caagtgaatt gtgagtctaa cacgggagga
     8401 caaagctagg ctcttgcaag gacagagcac acgtggatca tgctgtatga gctcactttg
     8461 tgtccctgga attagtgttt ttttgtgtgt gatctaactg gcaagtttaa tgaatagcca
     8521 aaatctatgt tattcttgag gctcctaaaa ttatgtgtga accccaagtt ctgtctagta
     8581 ttatatccct aaaatgcttt taatagtggt atgttacaag tggcataaga agatcctgtt
     8641 tttaaatttg tcaggcattt tattattcag tacataatat gtatagatct gagtgctgtt
     8701 ttccttgcat tgtctattat ttttaccttg tctttcccct aagagaattt gaagaaaatc
     8761 ttcaccaata tttctagtgt tgatggtaat taggaaaata gtagttgaga gatgtaagaa
     8821 tagtttgaga agagtgagta aaatgggtga aatgacaagt gggaaccatg tagctgagat
     8881 gtatttgttt agatgaaaag tctacactgg ttcttaatgt gaaaatcaaa ccatgtatct
     8941 accttaagaa caatgttgtt gtttttaatt tattgtagcg atttagtttt tttcacgtaa
     9001 tatttctatt ttctttgcag attgttttta atttaaattg tggaattttt acttctcaaa
     9061 atgtattaag tactgtattt tttgaaatct tatttttaaa taaatatttt ttaaaaacgt
     9121 a
//
