LOCUS       NM_001330059            5355 bp    mRNA    linear   PRI 06-MAR-2025
DEFINITION  Homo sapiens zDHHC palmitoyltransferase 20 (ZDHHC20), transcript
            variant 5, mRNA.
ACCESSION   NM_001330059 XM_006719795
VERSION     NM_001330059.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Zhang,H., Sun,Y., Wang,Z., Huang,X., Tang,L., Jiang,K. and Jin,X.
  TITLE     ZDHHC20-mediated S-palmitoylation of YTHDF3 stabilizes MYC mRNA to
            promote pancreatic cancer progression
  JOURNAL   Nat Commun 15 (1), 4642 (2024)
   PUBMED   38821916
  REMARK    GeneRIF: ZDHHC20-mediated S-palmitoylation of YTHDF3 stabilizes MYC
            mRNA to promote pancreatic cancer progression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Tomic,G., Sheridan,C., Refermat,A.Y., Baggelaar,M.P., Sipthorp,J.,
            Sudarshan,B., Ocasio,C.A., Suarez-Bonnet,A., Priestnall,S.L.,
            Herbert,E., Tate,E.W. and Downward,J.
  TITLE     Palmitoyl transferase ZDHHC20 promotes pancreatic cancer metastasis
  JOURNAL   Cell Rep 43 (5), 114224 (2024)
   PUBMED   38733589
  REMARK    GeneRIF: Palmitoyl transferase ZDHHC20 promotes pancreatic cancer
            metastasis.
REFERENCE   3  (bases 1 to 5355)
  AUTHORS   Lee,C.J., Stix,R., Rana,M.S., Shikwana,F., Murphy,R.E.,
            Ghirlando,R., Faraldo-Gomez,J.D. and Banerjee,A.
  TITLE     Bivalent recognition of fatty acyl-CoA by a human integral membrane
            palmitoyltransferase
  JOURNAL   Proc Natl Acad Sci U S A 119 (7) (2022)
   PUBMED   35140179
  REMARK    GeneRIF: Bivalent recognition of fatty acyl-CoA by a human integral
            membrane palmitoyltransferase.
REFERENCE   4  (bases 1 to 5355)
  AUTHORS   Carreras-Sureda,A., Abrami,L., Ji-Hee,K., Wang,W.A., Henry,C.,
            Frieden,M., Didier,M., van der Goot,F.G. and Demaurex,N.
  TITLE     S-acylation by ZDHHC20 targets ORAI1 channels to lipid rafts for
            efficient Ca2+ signaling by Jurkat T cell receptors at the immune
            synapse
  JOURNAL   Elife 10, e72051 (2021)
   PUBMED   34913437
  REMARK    GeneRIF: S-acylation by ZDHHC20 targets ORAI1 channels to lipid
            rafts for efficient Ca(2+) signaling by Jurkat T cell receptors at
            the immune synapse.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5355)
  AUTHORS   Kadry,Y.A., Lee,J.Y. and Witze,E.S.
  TITLE     Regulation of EGFR signalling by palmitoylation and its role in
            tumorigenesis
  JOURNAL   Open Biol 11 (10), 210033 (2021)
   PUBMED   34610265
  REMARK    GeneRIF: Regulation of EGFR signalling by palmitoylation and its
            role in tumorigenesis.
            Review article
REFERENCE   6  (bases 1 to 5355)
  AUTHORS   Del-Aguila,J.L., Beitelshees,A.L., Cooper-Dehoff,R.M.,
            Chapman,A.B., Gums,J.G., Bailey,K., Gong,Y., Turner,S.T.,
            Johnson,J.A. and Boerwinkle,E.
  TITLE     Genome-wide association analyses suggest NELL1 influences adverse
            metabolic response to HCTZ in African Americans
  JOURNAL   Pharmacogenomics J 14 (1), 35-40 (2014)
   PUBMED   23400010
REFERENCE   7  (bases 1 to 5355)
  AUTHORS   Ohno,Y., Kashio,A., Ogata,R., Ishitomi,A., Yamazaki,Y. and
            Kihara,A.
  TITLE     Analysis of substrate specificity of human DHHC protein
            acyltransferases using a yeast expression system
  JOURNAL   Mol Biol Cell 23 (23), 4543-4551 (2012)
   PUBMED   23034182
REFERENCE   8  (bases 1 to 5355)
  AUTHORS   Draper,J.M. and Smith,C.D.
  TITLE     DHHC20: a human palmitoyl acyltransferase that causes cellular
            transformation
  JOURNAL   Mol Membr Biol 27 (2-3), 123-136 (2010)
   PUBMED   20334580
  REMARK    GeneRIF: DHHC20 is a human N-terminal-myristoyl-directed palmitoyl
            acyltransferase involved in cellular transformation, that may play
            a role in cancer.
REFERENCE   9  (bases 1 to 5355)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   10 (bases 1 to 5355)
  AUTHORS   Ohno,Y., Kihara,A., Sano,T. and Igarashi,Y.
  TITLE     Intracellular localization and tissue-specific distribution of
            human and yeast DHHC cysteine-rich domain-containing proteins
  JOURNAL   Biochim Biophys Acta 1761 (4), 474-483 (2006)
   PUBMED   16647879
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DR003340.1, CR737535.1, AB040396.1, BC067898.1, AL136219.17 and
            BU685378.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001330059.1.
            
            Transcript Variant: This variant (5) uses an alternate in-frame
            splice site, contains an alternate exon in the 3' coding region and
            differs in its 3' UTR, compared to variant 1. The encoded isoform
            (3) is longer and has a distinct C-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.1774199.1,
                                           SRR7346977.2663492.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000400590.8/ ENSP00000383433.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-698               DR003340.1         44-741
            699-1028            CR737535.1         1-330               c
            1029-1136           AB040396.1         1-108               c
            1137-1551           BC067898.1         1366-1780
            1552-5335           AL136219.17        17854-21637         c
            5336-5355           BU685378.1         17-36               c
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q12.11"
     gene            1..5355
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /note="zDHHC palmitoyltransferase 20"
                     /db_xref="GeneID:253832"
                     /db_xref="HGNC:HGNC:20749"
                     /db_xref="MIM:617972"
     exon            1..250
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..1230
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /EC_number="2.3.1.225"
                     /note="isoform 3 is encoded by transcript variant 5;
                     probable palmitoyltransferase ZDHHC20; zinc finger
                     DHHC-type palmitoyltransferase 20; zinc finger DHHC
                     domain-containing protein 20; zinc finger DHHC-type
                     containing 20; acyltransferase ZDHHC20; DHHC
                     domain-containing cysteine-rich protein 20"
                     /codon_start=1
                     /product="palmitoyltransferase ZDHHC20 isoform 3"
                     /protein_id="NP_001316988.1"
                     /db_xref="CCDS:CCDS81758.1"
                     /db_xref="GeneID:253832"
                     /db_xref="HGNC:HGNC:20749"
                     /db_xref="MIM:617972"
                     /translation="MAPWTLWRCCQRVVGWVPVLFITFVVVWSYYAYVVELCVFTIFG
                     NEENGKTVVYLVAFHLFFVMFVWSYWMTIFTSPASPSKEFYLSNSEKERYEKEFSQER
                     QQEILRRAARALPIYTTSASKTIRYCEKCQLIKPDRAHHCSACDSCILKMDHHCPWVN
                     NCVGFSNYKFFLLFLLYSLLYCLFVAATVLEYFIKFWTNELTDTRAKFHVLFLFFVSA
                     MFFISVLSLFSYHCWLVGKNRTTIESFRAPTFSYGPDGNGFSLGCSKNWRQVFGDEKK
                     YWLLPIFSSLGDGCSFPTRLVGMDPEQASVTNQNEYARSSGSNQPFPIKPLSESKNRL
                     LDSESQWLENGAEEGIVKSGTNNHVTVAIEN"
     exon            251..277
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            278..381
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            382..502
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            503..572
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            573..605
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            606..726
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            727..859
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            860..986
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1076
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1192
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1270
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..5355
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5332..5337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /note="hexamer: AATAAA"
     regulatory      5336..5341
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /note="hexamer: AACAAA"
     polyA_site      5349
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
     polyA_site      5355
                     /gene="ZDHHC20"
                     /gene_synonym="4933421L13Rik; DHHC-20; DHHC20"
                     /note="major polyA site"
ORIGIN      
        1 gctcccacaa gtcctgcctc ggaggcgggg gagctggacc agcagccgcc tggagcgtcc
       61 gagtcaccgt cgccggggct cccgcgctcc ccagaacggt gggacgcggg gctcggcagc
      121 cgccagcgga acatggcgcc ctggacgctg tggcgctgct gccagcgcgt cgtgggctgg
      181 gtgccggtgc tcttcatcac cttcgtggtc gtctggtcct actacgcgta cgtggtggag
      241 ctctgcgtgt ttactatttt tggaaatgaa gaaaatggaa agaccgttgt ttaccttgtg
      301 gctttccatc tgttctttgt tatgtttgta tggtcctatt ggatgacaat tttcacatct
      361 cccgcttccc cctccaaaga gttctacttg tccaattctg aaaaggaacg ttatgaaaaa
      421 gaattcagcc aagaaagaca acaagaaatt ttgagaagag cagcaagagc tttacctatc
      481 tataccacat cagcttcaaa aactatcaga tattgtgaaa aatgtcagct gattaaacct
      541 gatcgggcgc atcactgctc agcctgtgac tcatgtattc ttaagatgga tcatcactgt
      601 ccttgggtga ataactgtgt gggattttct aattacaaat tcttcctgct gtttttattg
      661 tattccctat tatattgcct tttcgtggct gcaacagttt tagagtactt tataaaattt
      721 tggacgaatg aactgacaga tacacgtgca aaattccacg tactttttct tttctttgtg
      781 tctgcaatgt tcttcatcag cgtcctctca cttttcagct accactgctg gctagttgga
      841 aaaaatagaa caacaataga atcattccgc gcacccacgt tttcatacgg acctgatgga
      901 aatggtttct ctcttggatg cagtaaaaat tggagacaag tctttggtga tgaaaagaaa
      961 tattggctac ttccaatatt ttcaagcttg ggtgatggtt gcagttttcc aactcgcctt
     1021 gtggggatgg atccagaaca agcttctgtt acaaaccaga atgagtatgc cagaagtagt
     1081 ggctcaaatc aaccttttcc tatcaaacca cttagtgaat caaaaaaccg cttgttggac
     1141 agtgaatctc agtggctgga gaatggagct gaagaaggca tcgtcaaatc agggacaaat
     1201 aaccatgtaa cagtggcaat agaaaattga gtaccacttg ttcataacta accatttgaa
     1261 taagagcaag gtgtatgaaa acattataga ctggtatttt caattttcat ttgcaagaaa
     1321 atgatcagtg gaatgaaata actgaagtat aacagaagat atatttttta aaacggaaag
     1381 cctttgtaca gttcctggga ttcacagaag cactactcca gagcagaatg atgccttaat
     1441 cttaagtgtc catttgtgca gcattgactt agagctacaa aagtgactta atgttattct
     1501 ggaaataata cttacctgtt atgagttgct ataatatgag ctgtcatcac attttaacat
     1561 gcatatgtat tttttgatac ctgaattaca ttattagaat aagtatccat atacattttc
     1621 actccaaaaa cacaagctta aaaactttaa agtacatcta gggcaaatgg tggctgaaag
     1681 tgaataatat tcaaattact gttgcttgta ctgtataagg aaaactgctt ttttaaattg
     1741 ggtatttgct aatttataat tactatttta tacttttcaa catttttaat aacttttttt
     1801 attgttggct ataacagaag gattcagata tctaaatatc atttagaaca tagaacacat
     1861 tggtctaatt catcgaaagc ttagttatga gcattaatac aaccatgtag tagagcaagt
     1921 gcccaaggtt taaagggaac ttggcagtat aagaaatatt ttgggttctc tctgctattt
     1981 tatagctttt tttgttgaac tttcaggggt atgtacatgg gcagagaaac acaaaggtac
     2041 atgtgctgga gaatactgga cacagtagga agaagtatag gtgtccagaa gaaaatctaa
     2101 gaggaatatt gtaatttgtt aaaaaaaata gttttgagtt ttaacagcag ttagaataaa
     2161 gcatgctgtt gacttcactt tcaaatttca gtgtaggtaa tgactaaaaa ttactttgaa
     2221 actttttaca gtaatactgt aatagcttaa acagtgattt aaaatgcaat ttaaactagg
     2281 caaaaaaaac tttagagtaa gttttcctcc tggctgtcag ttggaaattc taacctgagt
     2341 ggtcatttta cattcccagt ctcttgagaa gtggattcta ctcttagacg cacagtctga
     2401 agtctgactt cctaacggca acccctttat gactagacac atttctcctt tttcctttta
     2461 aaatctaagc gacaggaggt gttgcattaa ctccaaggaa aagtacagaa taaacaatgt
     2521 agaaatgtca ctcctcattt acacctttta gacagacaga cacacacaca cacacacccc
     2581 ccccaaaata attgattgct tccagtgagt gggtggagat gaatctcagc agcatctgtg
     2641 agtactacag gttggagctt cctgtctgta aaatgggact gcaggggagg gggtgttgtg
     2701 aataagttgg actggatgac ttctaacatt ctgtgatgcc taattttgca aaatcacttt
     2761 tcattcaccc aataaatttt tttcttcttt tttccacaga gttttgctct gtctcccagg
     2821 caggagtgca gtggcgggat cttggctcgc tgcaacctct gccttccagg ttcaatagag
     2881 tctcctgcct cagcctccca agtagctggg attacaggct catgccacca tgcccggcta
     2941 attttcacat ttttagaaga gacgaggttt caccatgttg accaggctga tcactaactc
     3001 ctgacctcaa ataatccacc cacctcagct tcccaaagtg ctgggattac aggcgtgagc
     3061 caccacgccc cgccccggta aattttttgg agtaccttct gtatgctaaa taataaacta
     3121 ggaatagctc tgtcatttcc tagtacagca taaattttat ggttgctact gtaggtttat
     3181 aatttgttta taatttggcc taatttccat cagccatact aatattggat tttaaaagga
     3241 ggcaactttt tttctttttg aaccaaagga atgagttagc tttgaaaaca taatttggga
     3301 tattatagta tggattcttc tacctttgtt tcaactcctt agtaatcttg attctagcaa
     3361 actagaatga gatttaagag ataccaatta ttgctgggtg tggtggctca tgcctgtaat
     3421 cccagcactt tgggaggcca aggctggcgg atcacctgag gtcaggggtt cgagattagc
     3481 ctggtcaaaa tggcaaaacc ctgtctccac taaaaataca aaaaaacccc aaaactgtcc
     3541 aggcatggtg gcacacgcct gtagtcccaa ctactcggga ggtggaggca ggagaatcac
     3601 ttgaacccag gaggtggagg tcgcagtgag ccgagatcgt gccactgcac tccagcctgg
     3661 gcgacgagtg aaactccatc tcaaaaatat atatatatat caattaccaa ctaaaaacat
     3721 aactccagtt tggcagtttg catattataa ggagataaat gttaaaacat acttgactac
     3781 tttcagaaat gttctcctgg tactttttgc atttctacat tcagataaaa agatttgcat
     3841 gcacctggct aacgccaagg gaacttcatt tttttcttca ctattatgca ctttcatggt
     3901 atagtctttc tcagttcttt taatttttgt tatttaacat ctttaatagc acagcaaaca
     3961 tcttttcaga aattttcagt taaagccttt gaattactta tctttgattt aatttacagc
     4021 cagcattttg ccacgttcta aataatattt agctcaactg attcatacgt attaatgacc
     4081 attctagcaa aggcctacaa gtggtgtggg aatcagggaa aggctgcctc tttggtatct
     4141 caactggtat tgattattgc tatcaactat ttggggagaa aaaatcaaaa tgaagccctg
     4201 tcaaatttta gaagtactat ctttggtcct tcaaacactt tgtgatgaca ccttaagaaa
     4261 aataaagttg aagttcaggt cttgccattg ccattacaga caaattagga gacttggttt
     4321 acctgggaac aaatttactt gaatattcag tacctgaaac tatgccaaac caaagagcag
     4381 ctgcagtaca ttcgttattt taaatgaaca agtttacaaa gtttattttc atctatacgt
     4441 aaggatgatt tttttaaaac tttttacata ttagtggtta tgatccaatg tgtcatgagt
     4501 gaatttaact gtaaggtggt ttaaatcaaa tatgcaatgt ttacttgaat tgtatttcta
     4561 ttagcagatt ttgactatgt ttacaggacg gtttaaatta aggattatca ggcatgtgag
     4621 atctttcagt tatctttaaa gtagatgtat attaagggct tagatttagg atctacatat
     4681 tctgggcatt gaataggcag taacttacaa ataagttttg cttacctttt gttctaggga
     4741 ctagcactgc tatcaatgga aagtattttt aactaatctg ttattaagaa agtcatattt
     4801 ttgcatttca gccaaaataa agaccgcctg taataatctg ttagaaacag ataatacatg
     4861 tctgaaatcc atatgtttca tatgatctaa actgtatttt ccaatttaaa ttaaaaatgt
     4921 aatatagatt cagaaaggtt catatttttc taatgacttc attctatatt attttgttag
     4981 gttgcataaa gaagcaagga attgtacttg tattaaaaga tgaagaaagc tattaggtat
     5041 atttgtacat gactgcaaat gagtctatgc ccgtttaaaa gaaaagatgg acactatttt
     5101 aaagtgagct ttaatatgct tttatataaa caaatttgaa gtacagttta gtttggttgt
     5161 gtttacctaa caagtaccat aagccttgtg tttgttctta tttgtataat cctagcctgt
     5221 gacttaatgt tgatgctttg ctttgtcttt tggctggcct aacctacatt gacatgtaca
     5281 cagaacattt taaaactttt tttttcaaaa gtcataatga attactttat taataaacaa
     5341 agtcttgtat ttgaa
//
