LOCUS       NM_144969               5578 bp    mRNA    linear   PRI 06-MAR-2025
DEFINITION  Homo sapiens zDHHC palmitoyltransferase 15 (ZDHHC15), transcript
            variant 1, mRNA.
ACCESSION   NM_144969
VERSION     NM_144969.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Liu,Z.Y., Lan,T., Tang,F., He,Y.Z., Liu,J.S., Yang,J.Z., Chen,X.,
            Wang,Z.F. and Li,Z.Q.
  TITLE     ZDHHC15 promotes glioma malignancy and acts as a novel prognostic
            biomarker for patients with glioma
  JOURNAL   BMC Cancer 23 (1), 420 (2023)
   PUBMED   37161425
  REMARK    GeneRIF: ZDHHC15 promotes glioma malignancy and acts as a novel
            prognostic biomarker for patients with glioma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Casellas-Vidal,D., Mademont-Soler,I., Sanchez,J., Plaja,A.,
            Castells,N., Camos,M., Nieto-Moragas,J., Del Mar Garcia,M.,
            Rodriguez-Solera,C., Rivera,H., Brunet,J., Alvarez,S., Perapoch,J.,
            Queralt,X. and Obon,M.
  TITLE     ZDHHC15 as a candidate gene for autism spectrum disorder
  JOURNAL   Am J Med Genet A 191 (4), 941-947 (2023)
   PUBMED   36565021
  REMARK    GeneRIF: ZDHHC15 as a candidate gene for autism spectrum disorder.
REFERENCE   3  (bases 1 to 5578)
  AUTHORS   Fan,X., Yang,H., Zhao,C., Hu,L., Wang,D., Wang,R., Fang,Z. and
            Chen,X.
  TITLE     Local anesthetics impair the growth and self-renewal of
            glioblastoma stem cells by inhibiting ZDHHC15-mediated GP130
            palmitoylation
  JOURNAL   Stem Cell Res Ther 12 (1), 107 (2021)
   PUBMED   33541421
  REMARK    GeneRIF: Local anesthetics impair the growth and self-renewal of
            glioblastoma stem cells by inhibiting ZDHHC15-mediated GP130
            palmitoylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5578)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 5578)
  AUTHORS   Greaves,J., Munro,K.R., Davidson,S.C., Riviere,M., Wojno,J.,
            Smith,T.K., Tomkinson,N.C. and Chamberlain,L.H.
  TITLE     Molecular basis of fatty acid selectivity in the zDHHC family of
            S-acyltransferases revealed by click chemistry
  JOURNAL   Proc Natl Acad Sci U S A 114 (8), E1365-E1374 (2017)
   PUBMED   28167757
REFERENCE   6  (bases 1 to 5578)
  AUTHORS   Greaves,J., Salaun,C., Fukata,Y., Fukata,M. and Chamberlain,L.H.
  TITLE     Palmitoylation and membrane interactions of the neuroprotective
            chaperone cysteine-string protein
  JOURNAL   J Biol Chem 283 (36), 25014-25026 (2008)
   PUBMED   18596047
REFERENCE   7  (bases 1 to 5578)
  AUTHORS   Ohno,Y., Kihara,A., Sano,T. and Igarashi,Y.
  TITLE     Intracellular localization and tissue-specific distribution of
            human and yeast DHHC cysteine-rich domain-containing proteins
  JOURNAL   Biochim Biophys Acta 1761 (4), 474-483 (2006)
   PUBMED   16647879
REFERENCE   8  (bases 1 to 5578)
  AUTHORS   Mansouri,M.R., Marklund,L., Gustavsson,P., Davey,E., Carlsson,B.,
            Larsson,C., White,I., Gustavson,K.H. and Dahl,N.
  TITLE     Loss of ZDHHC15 expression in a woman with a balanced translocation
            t(X;15)(q13.3;cen) and severe mental retardation
  JOURNAL   Eur J Hum Genet 13 (8), 970-977 (2005)
   PUBMED   15915161
REFERENCE   9  (bases 1 to 5578)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
REFERENCE   10 (bases 1 to 5578)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK122885.1, AK056374.1,
            AK128587.1, AL137013.4, AK123754.1 and BQ028106.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_144969.2.
            
            Summary: The protein encoded by this gene belongs to the DHHC
            palmitoyltransferase family. Mutations in this gene are associated
            with mental retardatio X-linked type 91 (MRX91). Alternatively
            spliced transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Sep 2009].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.239163.1,
                                           SRR1660803.139798.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373367.8/ ENSP00000362465.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-149               AK122885.1         215-363
            150-1779            AK056374.1         153-1782
            1780-2133           AK128587.1         1753-2106
            2134-2134           AL137013.4         57887-57887
            2135-3456           AK128587.1         2108-3429
            3457-3704           AL137013.4         59210-59457
            3705-4356           AK123754.1         883-1534
            4357-4926           AL137013.4         60110-60679
            4927-5578           BQ028106.1         15-666              c
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13.3"
     gene            1..5578
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /note="zDHHC palmitoyltransferase 15"
                     /db_xref="GeneID:158866"
                     /db_xref="HGNC:HGNC:20342"
                     /db_xref="MIM:300576"
     exon            1..149
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..1027
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /EC_number="2.3.1.225"
                     /note="isoform 1 is encoded by transcript variant 1; zinc
                     finger, DHHC domain containing 15; palmitoyltransferase
                     ZDHHC15; DHHC-15; zinc finger DHHC domain-containing
                     protein 15; zinc finger DHHC-type containing 15;
                     acyltransferase ZDHHC15; zinc finger DHHC-type
                     palmitoyltransferase 15"
                     /codon_start=1
                     /product="palmitoyltransferase ZDHHC15 isoform 1"
                     /protein_id="NP_659406.1"
                     /db_xref="CCDS:CCDS14430.1"
                     /db_xref="GeneID:158866"
                     /db_xref="HGNC:HGNC:20342"
                     /db_xref="MIM:300576"
                     /translation="MRRGWKMALSGGLRCCRRVLSWVPVLVIVLVVLWSYYAYVFELC
                     LVTVLSPAEKVIYLILYHAIFVFFTWTYWKSIFTLPQQPNQKFHLSYTDKERYENEER
                     PEVQKQMLVDMAKKLPVYTRTGSGAVRFCDRCHLIKPDRCHHCSVCAMCVLKMDHHCP
                     WVNNCIGFSNYKFFLQFLAYSVLYCLYIATTVFSYFIKYWRGELPSVRSKFHVLFLLF
                     VACMFFVSLVILFGYHCWLVSRNKTTLEAFCTPVFTSGPEKNGFNLGFIKNIQQVFGD
                     KKKFWLIPIGSSPGDGHSFPMRSMNESQNPLLANEETWEDNEDDNQDYPEGSSSLAVE
                     TET"
     exon            150..176
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            177..271
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            272..392
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            393..462
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            463..495
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            496..616
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            617..749
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            750..876
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            877..980
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1059
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..5578
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1819..1824
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /note="hexamer: AGTAAA"
     polyA_site      1842
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
     regulatory      5558..5563
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /note="hexamer: ATTAAA"
     polyA_site      5578
                     /gene="ZDHHC15"
                     /gene_synonym="DHHC15; MRX91"
                     /note="major polyA site"
ORIGIN      
        1 cttcgagcca aagatgcggc gaggctggaa gatggctctg tctggggggc tgcggtgctg
       61 ccgccgggta ctgtcctggg tgccagtgct cgttattgtc ctcgtcgtgc tctggtccta
      121 ctatgcctac gtctttgaac tctgcctggt gactgttttg agcccagcag aaaaagttat
      181 ttacctcata ctctaccatg ccatctttgt gttctttacc tggacctact ggaagtctat
      241 ctttacactc ccacagcagc caaaccagaa gttccacttg tcctacacag acaaggagcg
      301 ctatgaaaat gaagaaagac ctgaggtcca gaagcagatg cttgttgata tggccaaaaa
      361 gctaccggtt tacacaagaa ctggaagtgg agctgtacga ttctgtgacc ggtgtcatct
      421 gatcaagcca gaccgctgcc accactgctc tgtctgtgct atgtgtgtgt taaaaatgga
      481 tcatcactgc ccttgggtta ataactgcat tggattttcc aactacaaat tcttccttca
      541 attcttagct tactctgttc tctactgcct gtacattgct acgacagtct tcagctattt
      601 catcaaatac tggagagggg aattacccag tgttcgctct aagttccatg tcctttttct
      661 tctctttgtg gcctgcatgt tttttgtcag ccttgtgatt ctctttggtt accattgttg
      721 gcttgtcagc agaaacaaaa ccaccttaga ggccttctgc actccagtgt ttacaagtgg
      781 cccagagaaa aatgggttca accttggctt catcaagaat atccagcagg tgtttggaga
      841 taagaagaag ttctggttaa tacctattgg ttccagccct ggtgatggac actccttccc
      901 tatgaggtct atgaatgagt cacagaaccc actgctagca aatgaagaaa cctgggaaga
      961 caacgaggat gacaaccaag attatccaga aggctcatca tctcttgctg tggaaacgga
     1021 aacatagcag ttttcacatt tcctgcatct ctcagacagg actcaccatc tctgcctccc
     1081 atgaggctta cagagttcaa tgttggaaat cattgtaatc ttcaaaataa gtcaccgtgt
     1141 tggattgaaa gcttcaaaat ttgaaagaat tccatcaaat acttgctgtg taaatgtttc
     1201 tggactttat gttatttaat ttactgactg aaatccaatt tggaatttgg tagcagttaa
     1261 ttcaagccaa ttttttttgt ttcttcattt cccctccccc aatccatgaa agcctaaatg
     1321 taaaatatat cttttcattc atcttatcag gtaaaaggaa attcagaaaa tttccttaga
     1381 gtctttaatt cccccacaaa gattattaat cacatatata gggccttttt ggtgttgaag
     1441 ggaatcaaac tacatttgct gcttgtgtgc gtgtgcattt gtgaacacgt acagcatatc
     1501 tatacaaaat tctgctatag tgtgaaaatc agggctaaaa acctgaagcc tttgtttaat
     1561 tatgcttttc ctctaaatag caacttaaat atttgctaga ctttgaatca tcgctatatc
     1621 aagtatctaa aatttgggag ggtgaatcag tacactgtga ccaaggtcct caaattggaa
     1681 tttgaacaac aatgtaaaac ctgttctgtc acaaatgttc ctgaaagcac cacagctact
     1741 caagaagatc aaattcagga cataaacttt attgaacata ataaccagtg ttttttaaag
     1801 acttttttgt cagggtgcag taaaattctc caattacttt tattcattta tctgcttcct
     1861 agttttattt tagcctttat gagttatttt atgatgaaag aaaatctata agcctaagtt
     1921 atggcccata ggttttgtta agggttttca gagtgatttt caggtttaat ttcactgcct
     1981 ttcataaggg taaagtttca cagctgacat tagcctgagg attagcacta aagagctgtg
     2041 ttttcatagc aggggctatt gtacagacag tgataaccaa atagtaatat gccagttctt
     2101 tcttcttttt ttagcttcag tagtagctaa gccattaata cagtttgcat caatttcccc
     2161 atttatttat aatcagcctt tcttttagtc ctagtgcctg aatcaatgag tgagaatact
     2221 agtactgatt ctttacaata ataatatctt aagagtattg atatctttca ttttttcatc
     2281 cagcagtcta gtgcccagct gctgacaata aaagctccaa ctcttttaga gatcagtgga
     2341 atttagaagc atcttacagg tatcaaggaa ttgagcttct agagggatgg tttggcttac
     2401 taatttatct tccctgaaat atgtttgagt aaaaaagagt tgcctgccac aatttagtgt
     2461 tggggttcca gtggagctga gaaagtgaac aatccagcta cttaaagcaa caacaaaacc
     2521 tgttcatctg gatccctagg taattttgac caaggtgaga cttttggcat aaattaaatg
     2581 gattgtggtc agcattacat ttgagagaat actagacatg actacactta ttagggctag
     2641 tgactttctc aaactcagaa gctaaaaagc agcctagatg actatgcaca cactgggttt
     2701 caagatgaaa tcttcttata tattcagatg gaaaaaaatt tccccttaca ctctttctca
     2761 aaatactctc atttcggttt ttcctgaaac cttatttggc cattcccatg gttttatgac
     2821 agacaagcct tgtcttctat tcatatagat atgcaccttt ttctacatgt atggccttta
     2881 cttactacac agcaagtagg cctcaaagta atcttcctct ttcccatcaa tcatcttctg
     2941 ccaagcattc aggcagagca gctgttaact gtgattcaaa gtcagaaaaa catcaagaac
     3001 ttctttgatg catagggtgt atcagcactc agctcccaca gaagtcaagt cacaaaatat
     3061 agtgtcagtt aactataagg aagttccgga gaggtaaagc tcaggaaaga tgtaatgcaa
     3121 agctgattgt ttaatgtctt acagatttac actgtcttta aaaggccaaa taaggctggg
     3181 cgcagtggct cacacctgta atcccaacat tttgggagcc caaggcggcc ggaccacctg
     3241 aggtcgggag ttcaagacca gcctgaccaa catggagaaa ccctgtctct actaaaaata
     3301 caaaaattag ccaggagtgt tggtgcatga ctgtaatccc agctactcag gaggctgagg
     3361 caggagaatc gcttgaacct gggaggtaga ggttgtggtg agctgagatc gtgccattgc
     3421 actccggcct gggcaataag agtgaaactc tgtctcaaaa aaaaaaaaaa aaaaaaaaaa
     3481 agccaaataa atcagggttt taccaggggc tcagagataa aaagaaaacc tattcctatc
     3541 ttagcatttt ggtctcattg gtttatagac tctgataata tccctatcca tggatcattt
     3601 gagtattgac taatgtacat aggtacccaa ctggttgatt attaatcaag aatacagaac
     3661 tttatttaat aattagcaca ccctgatctg gcttctttgc ttgcttttct ttggatccct
     3721 gtccaataac ctctgtggaa tctcattccc taattgttga tactcatttt ctccgtttgc
     3781 aaaatgaaga tactcattgt taggatccta ctgctgaagg gtgctatgtc actttctagt
     3841 gctcttatat gtctttaatt ataggactgg aatgaaaagc ttaaccatac agtatttcta
     3901 agcaaaataa tgtactccat tcttttgtcc atgctctcat tgctgatttg ttcttgcagg
     3961 acttagagca ctttgttaat gttcttccat tcactccaac agcgtcctca gaaagagaca
     4021 gaataaaaaa attgttcccc atggtacagc tggaaaaatt aaggggaaaa gagattaagt
     4081 ggtccccaat ggaacacagc tgtcttatgc agggctcaca gttaccatgt tcagtacatt
     4141 gttaaagtaa cttggcttcc caggactgga attatcatgg agcagttaga attttgagag
     4201 taattacaac aggatcacat tctgaaagag tcacattttg tcaaaaatct gaaaagtatg
     4261 caatttgtac aactcttact ttctatgtga taagaatgca atatgtgcct ctttccccta
     4321 ctagccccat gtgacacata tttttcacac tatgtggcat caaccagtgc cattacctcc
     4381 tgtggcacca tagagcccaa tctcagggca gacattgcca aggtaaagaa tgcactccat
     4441 gtttatacca aattgcacaa agtatagaca ttgaggttaa tggagattta agcacaatcc
     4501 tagcaagtaa gttttaagaa tactgaatat tatacattat tgtaacatga acctaaaatt
     4561 aatttaacag ggcccaaatt cataggaatg taacagtcat tgaaagccta ttcttttcac
     4621 ctctcatcac acatatctac atacatgtat gcacatgtac acacacacac acacacacac
     4681 acacacacat acacattttc tccaaagcag gctgcctctt cagcaggagc aaatacttta
     4741 atttaaaaga atgattatct gttttctcct tttagaaaaa gagtctaaga aacacagcat
     4801 gtgaagaaca ttagatttat acattccaga atctctgctc tcagagtagc taagagattc
     4861 ggtacagaag acgttctatg gagaagcaca gggggcatct aatgagattg tcaggccctt
     4921 ggacaagatc catattctta gaaaattaat gagttccatc tcgttaggaa ttggttgatg
     4981 aggtatggta catctgcatt tctttaacca tagctgtaat gtaccatatt aaattgtagc
     5041 cttttgcatg cgggtattat ataataatga atgggtggct cctagtagca tgcaacccat
     5101 gactgtttac ttccctaaaa gggttaggtc tgtgtcctgc acagtgttca aaaccattaa
     5161 atgtgttctt tgtagcagga tggtacacag ccatcagcag aatgagttaa aaccaacaat
     5221 gaattcaata taccaggaaa tagattgtta gtagggaatg gcattaaagg aaaaaaaaat
     5281 tagataagat gcattcaagg aataattact ttcactctgc tggcttaatc agtgaaggaa
     5341 cataaaagga aagctggcag tgggtcttgg taatatatga actatattag agtgccagaa
     5401 aaggagacct cagaagttaa gcataggttg ccaatctgct acaattattt ttcccaccaa
     5461 tctgctcaaa atgattgtat atagtattct taaaaccatg tgtcttggtg cagaaatatt
     5521 gacaaatgct ggccagcttt atcttcctat atccttcatt aaaagtatat gcatataa
//
