LOCUS       NM_003386               8700 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens zonadhesin (ZAN), transcript variant 3, coding, mRNA.
ACCESSION   NM_003386
VERSION     NM_003386.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Roberts,E.K., Tardif,S., Wright,E.A., Platt,R.N. 2nd, Bradley,R.D.
            and Hardy,D.M.
  TITLE     Rapid divergence of a gamete recognition gene promoted
            macroevolution of Eutheria
  JOURNAL   Genome Biol 23 (1), 155 (2022)
   PUBMED   35821049
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Tardif,S. and Cormier,N.
  TITLE     Role of zonadhesin during sperm-egg interaction: a species-specific
            acrosomal molecule with multiple functions
  JOURNAL   Mol Hum Reprod 17 (11), 661-668 (2011)
   PUBMED   21602212
  REMARK    Review article
REFERENCE   3  (bases 1 to 8700)
  AUTHORS   Tardif,S., Brady,H.A., Breazeale,K.R., Bi,M., Thompson,L.D.,
            Bruemmer,J.E., Bailey,L.B. and Hardy,D.M.
  TITLE     Zonadhesin D3-polypeptides vary among species but are similar in
            Equus species capable of interbreeding
  JOURNAL   Biol Reprod 82 (2), 413-421 (2010)
   PUBMED   19794156
REFERENCE   4  (bases 1 to 8700)
  AUTHORS   Herlyn,H. and Zischler,H.
  TITLE     The molecular evolution of sperm zonadhesin
  JOURNAL   Int J Dev Biol 52 (5-6), 781-790 (2008)
   PUBMED   18649290
  REMARK    Review article
REFERENCE   5  (bases 1 to 8700)
  AUTHORS   Herlyn,H. and Zischler,H.
  TITLE     Sequence evolution of the sperm ligand zonadhesin correlates
            negatively with body weight dimorphism in primates
  JOURNAL   Evolution 61 (2), 289-298 (2007)
   PUBMED   17348940
REFERENCE   6  (bases 1 to 8700)
  AUTHORS   Gasper,J. and Swanson,W.J.
  TITLE     Molecular population genetics of the gene encoding the human
            fertilization protein zonadhesin reveals rapid adaptive evolution
  JOURNAL   Am J Hum Genet 79 (5), 820-830 (2006)
   PUBMED   17033959
  REMARK    GeneRIF: VWD2, membrane/A5 antigen mu receptor, and mucin-like
            domains in zonadhesin are rapidly evolving and, thus, may be
            involved in binding to the ZP in a species-specific manner
REFERENCE   7  (bases 1 to 8700)
  AUTHORS   Herlyn,H. and Zischler,H.
  TITLE     Identification of a positively evolving putative binding region
            with increased variability in posttranslational motifs in
            zonadhesin MAM domain 2
  JOURNAL   Mol Phylogenet Evol 37 (1), 62-72 (2005)
   PUBMED   15927490
REFERENCE   8  (bases 1 to 8700)
  AUTHORS   Wilson,M.D., Riemer,C., Martindale,D.W., Schnupf,P., Boright,A.P.,
            Cheung,T.L., Hardy,D.M., Schwartz,S., Scherer,S.W., Tsui,L.C.,
            Miller,W. and Koop,B.F.
  TITLE     Comparative analysis of the gene-dense ACHE/TFR2 region on human
            chromosome 7q22 with the orthologous region on mouse chromosome 5
  JOURNAL   Nucleic Acids Res 29 (6), 1352-1365 (2001)
   PUBMED   11239002
REFERENCE   9  (bases 1 to 8700)
  AUTHORS   Glockner,G., Scherer,S., Schattevoy,R., Boright,A., Weber,J.,
            Tsui,L.C. and Rosenthal,A.
  TITLE     Large-scale sequencing of two regions in human chromosome 7q22:
            analysis of 650 kb of genomic sequence around the EPO and CUTL1
            loci reveals 17 genes
  JOURNAL   Genome Res 8 (10), 1060-1073 (1998)
   PUBMED   9799793
REFERENCE   10 (bases 1 to 8700)
  AUTHORS   Gao,Z., Harumi,T. and Garbers,D.L.
  TITLE     Chromosome localization of the mouse zonadhesin gene and the human
            zonadhesin gene (ZAN)
  JOURNAL   Genomics 41 (1), 119-122 (1997)
   PUBMED   9126492
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009488.5, AF332977.1 and
            AK302596.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_003386.2.
            
            Summary: This gene encodes a protein that functions in the species
            specificity of sperm adhesion to the egg zona pellucida. The
            encoded protein is located in the acrosome and may be involved in
            signaling or gamete recognition. An allelic polymorphism in this
            gene results in both functional and frameshifted alleles; the
            reference genome represents the functional allele. Alternative
            splicing of this gene results in multiple transcript variants.
            [provided by RefSeq, Jul 2015].
            
            Transcript Variant: This variant (3) encodes the longer isoform
            (3).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF332977.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1965299,
                                           SAMEA1968968 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000613979.5/ ENSP00000480750.1
            polymorphic pseudogene :: PMID: 17033959
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AC009488.5         74405-74435
            32-66               AF332977.1         1-35
            67-67               AC009488.5         74849-74849
            68-5960             AF332977.1         37-5929
            5961-6102           AK302596.1         1360-1501
            6103-8477           AF332977.1         6072-8446
            8478-8690           AK302596.1         3463-3675
            8691-8700           AF332977.1         8660-8669
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..8700
                     /gene="ZAN"
                     /note="zonadhesin"
                     /db_xref="GeneID:7455"
                     /db_xref="HGNC:HGNC:12857"
                     /db_xref="MIM:602372"
     exon            1..54
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            55..249
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..8635
                     /gene="ZAN"
                     /note="isoform 3 precursor is encoded by transcript
                     variant 3, coding"
                     /codon_start=1
                     /product="zonadhesin isoform 3 precursor"
                     /protein_id="NP_003377.2"
                     /db_xref="CCDS:CCDS47664.2"
                     /db_xref="GeneID:7455"
                     /db_xref="HGNC:HGNC:12857"
                     /db_xref="MIM:602372"
                     /translation="MVPPVWTLLLLVGAALFRKEKPPDQKLVVRSSRDNYVLTQCDFE
                     DDAKPLCDWSQVSADDEDWVRASGPSPTGSTGAPGGYPNGEGSYLHMESNSFHRGGVA
                     RLLSPDLWEQGPLCVHFAHHMFGLSWGAQLRLLLLSGEEGRRPDVLWKHWNTQRPSWM
                     LTTVTVPAGFTLPTRLMFEGTRGSTAYLDIALDALSIRRGSCNRVCMMQTCSFDIPND
                     LCDWTWIPTASGAKWTQKKGSSGKPGVGPDGDFSSPGSGCYMLLDPKNARPGQKAVLL
                     SPVSLSSGCLSFSFHYILRGQSPGAALHIYASVLGSIRKHTLFSGQPGPNWQAVSVNY
                     TAVGRIQFAVVGVFGKTPEPAVAVDATSIAPCGEGFPQCDFEDNAHPFCDWVQTSGDG
                     GHWALGHKNGPVHGMGPAGGFPNAGGHYIYLEADEFSQAGQSVRLVSRPFCAPGDICV
                     EFAYHMYGLGEGTMLELLLGSPAGSPPIPLWKRVGSQRPYWQNTSVTVPSGHQQPMQL
                     IFKGIQGSNTASVVAMGFILINPGTCPVKVLPELPPVSPVSSTGPSETTGLTENPTIS
                     TKKPTVSIEKPSVTTEKPTVPKEKPTIPTEKPTISTEKPTIPSEKPNMPSEKPTIPSE
                     KPTILTEKPTIPSEKPTIPSEKPTISTEKPTVPTEEPTTPTEETTTSMEEPVIPTEKP
                     SIPTEKPSIPTEKPTISMEETIISTEKPTISPEKPTIPTEKPTIPTEKSTISPEKPTT
                     PTEKPTIPTEKPTISPEKPTTPTEKPTISPEKLTIPTEKPTIPTEKPTIPTEKPTIST
                     EEPTTPTEETTISTEKPSIPMEKPTLPTEETTTSVEETTISTEKLTIPMEKPTISTEK
                     PTIPTEKPTISPEKLTIPTEKLTIPTEKPTIPIEETTISTEKLTIPTEKPTISPEKPT
                     ISTEKPTIPTEKPTIPTEETTISTEKLTIPTEKPTISPEKLTIPTEKPTISTEKPTIP
                     TEKLTIPTEKPTIPTEKPTIPTEKLTALRPPHPSPTATGLAALVMSPHAPSTPMTSVI
                     LGTTTTSRSSTERCPPNARYESCACPASCKSPRPSCGPLCREGCVCNPGFLFSDNHCI
                     QASSCNCFYNNDYYEPGAEWFSPNCTEHCRCWPGSRVECQISQCGTHTVCQLKNGQYG
                     CHPYAGTATCLVYGDPHYVTFDGRHFGFMGKCTYILAQPCGNSTDPFFRVTAKNEEQG
                     QEGVSCLSKVYVTLPESTVTLLKGRRTLVGGQQVTLPAIPSKGVFLGASGRFVELQTE
                     FGLRVRWDGDQQLYVTVSSTYSGKLCGLCGNYDGNSDNDHLKLDGSPAGDKEELGNSW
                     QTDQDEDQECQKYQVVNSPSCDSSLQSSMSGPGFCGRLVDTHGPFETCLLHVKAASFF
                     DSCMLDMCGFQGLQHLLCTHMSTMTTTCQDAGHAVKPWREPHFCPMACPPNSKYSLCA
                     KPCPDTCHSGFSGMFCSDRCVEACECNPGFVLSGLECIPRSQCGCLHPAGSYFKVGER
                     WYKPGCKELCVCESNNRIRCQPWRCRAQEFCGQQDGIYGCHAQGAATCTASGDPHYLT
                     FDGALHHFMGTCTYVLTRPCWSRSQDSYFVVSATNENRGGILEVSYIKAVHVTVFDLS
                     ISLLRGCKVMLNGHRVALPVWLAQGRVTIRLSSNLVLLYTNFGLQVRYDGSHLVEVTV
                     PSSYGGQLCGLCGNYNNNSLDDNLRPDRKLAGDSMQLGAAWKLPESSEPGCFLVGGKP
                     SSCQENSMADAWNKNCAILINPQGPFSQCHQVVPPQSSFASCVHGQCGTKGDTTALCR
                     SLQAYASLCAQAGQAPAWRNRTFCPMRCPPGSSYSPCSSPCPDTCSSINNPRDCPKAL
                     PCAESCECQKGHILSGTSCVPLGQCGCTDPAGSYHPVGERWYTENTCTRLCTCSVHNN
                     ITCFQSTCKPNQICWALDGLLHCRASGVGVCQLPGESHYVSFDGSNHSIPDACTLVLV
                     KVCHPAMALPFFKISAKHEKEEGGTEAFRLHEVYIDIYDAQVTLQKGHRVLINSKQVT
                     LPAISQIPGVSVKSSSIYSIVNIKIGVQVKFDGNHLLEIEIPTTYYGKVCGMCGNFND
                     EEEDELMMPSDEVANSDSEFVNSWKDKDIDPSCQSLLVDEQQIPAEQQENPSGNCRAA
                     DLRRAREKCEAALRAPVWAQCASRIDLTPFLVDCANTLCEFGGLYQALCQALQAFGAT
                     CQSQGLKPPLWRNSSFCPLECPAYSSYTNCLPSCSPSCWDLDGRCEGAKVPSACAEGC
                     ICQPGYVLSEDKCVPRSQCGCKDAHGGSIPLGKSWVSSGCTEKCVCTGGAIQCGDFRC
                     PSGSHCQLTSDNSNSNCVSDKSEQCSVYGDPRYLTFDGFSYRLQGRMTYVLIKTVDVL
                     PEGVEPLLVEGRNKMDPPRSSIFLQEVITTVYGYKVQLQAGLELVVNNQKMAVPYRPN
                     EHLRVTLWGQRLYLVTDFELVVSFGGRKNAVISLPSMYEGLVSGLCGNYDKNRKNDMM
                     LPSGALTQNLNTFGNSWEVKTEDALLRFPRAIPAEEEGQGAELGLRTGLQVSECSPEQ
                     LASNSTQACRVLADPQGPFAACHQTVAPEPFQEHCVLDLCSAQDPREQEELRCQVLSG
                     HGVSSRYHISELYDTLPSILCQPGRPRGLRGPLRGRLRQHPRLCLQWHPEPPLADCGC
                     TSNGIYYQLGSSFLTEDCSQRCTCASSRILLCEPFSCRAGEVCTLGNHTQGCFPESPC
                     LQNPCQNDGQCREQGATFTCECEVGYGGGLCMEPRDAPPPRKPASNLVGVLLGLLVPV
                     VVVLLAVTRECIYRTRRKREKTQEGDRLARLVDTDTVLDCAC"
     exon            250..302
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            303..449
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            450..721
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            722..809
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            810..962
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1127
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1219
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1298
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1445
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1717
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1802
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..3320
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            3321..3505
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            3506..3647
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            3648..3767
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            3768..3892
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            3893..4038
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            4039..4182
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            4183..4293
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            4294..4463
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            4464..4666
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            4667..4808
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            4809..5056
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            5057..5237
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            5238..5349
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            5350..5444
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            5445..5621
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            5622..5830
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            5831..5975
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            5976..6223
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            6224..6388
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            6389..6513
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            6514..6818
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            6819..7030
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            7031..7175
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            7176..7423
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            7424..7553
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            7554..7725
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            7726..7908
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            7909..7983
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            7984..8182
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            8183..8321
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            8322..8462
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            8463..8562
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            8563..8609
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
     exon            8610..8700
                     /gene="ZAN"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agactccatg gttgctattg aaaagctgga tgccatgttg atttctgatc cttgagcttt
       61 tctgtgtttc tctgttcatt cttcatggca tccttggaag gatgccaagc taaggaggcc
      121 aggggggaat aaaaggagtc caggctccca actgtgccct tcccctctcc cctgggagca
      181 accacttagg gtcctcatgg ttcctccagt ctggactctg ctgcttctgg tgggggctgc
      241 ccttttcagg aaagagaagc ctccggacca gaagctggtt gttcgcagct ctagggacaa
      301 ctatgtcctc acccagtgtg attttgagga tgacgccaaa cccctctgtg actggtccca
      361 agtgtccgca gacgatgaag actgggttcg agccagtggg ccctctccca ccggctccac
      421 cggggccccc ggggggtacc ctaacggaga gggcagctat ctgcatatgg aatcgaacag
      481 cttccaccgt gggggagtgg cccgcctgct cagccccgac ctatgggagc aaggccccct
      541 ctgtgtgcac tttgcccacc acatgttcgg gctgtcttgg ggcgcccagc tcaggctgct
      601 gctgctctcg ggtgaagagg gccgccgccc cgatgtgctc tggaaacact ggaacaccca
      661 gagaccctcc tggatgctca ccaccgtcac tgtgcccgca gggttcaccc tgcccacccg
      721 gctgatgttt gagggaacac ggggtagcac tgcctacctg gacatcgccc tggatgccct
      781 ctctatccgc cggggctcct gtaatcgcgt ctgtatgatg caaacatgca gctttgacat
      841 tccaaatgac ctctgtgact ggacctggat cccaactgcc tccggggcca agtggactca
      901 gaagaaaggg tcatcaggaa agccaggcgt ggggcctgat ggcgacttct ctagccctgg
      961 tagtggctgc tacatgctcc tggaccccaa gaatgcaaga cctgggcaga aagctgtcct
     1021 cctgagcccc gtgagcctgt cctctggctg tctgagcttt tccttccact acatcctccg
     1081 gggccagtct cctggtgcag ccctccacat ttatgcttca gtcttgggga gtatccggaa
     1141 acacactctc ttctcaggac aacctgggcc caactggcag gctgtttctg tcaattacac
     1201 agccgtggga cggatacagt ttgccgtggt aggcgttttt ggaaagaccc cagagccagc
     1261 tgtggcagtt gatgcaacca gcattgctcc ttgtggggag ggttttcctc agtgtgactt
     1321 tgaagacaac gcccatccct tctgtgactg ggtccagact tccggggatg gtggacactg
     1381 ggccctcgga cataaaaatg gacccgtcca tggcatgggc cctgcgggag gtttccctaa
     1441 tgcagggggt cactatatct accttgaggc tgacgagttc tcccaggcag gccagtcagt
     1501 cagactggtg agccggccct tctgcgcccc aggtgacatc tgcgtggagt tcgcatacca
     1561 catgtatggc cttggggagg gtactatgct cgaactcctc ctgggaagtc ctgcggggag
     1621 tcccccgatt cctctctgga aacgcgtggg gtctcagcgc ccttactggc agaacacctc
     1681 cgtcaccgtc ccctcaggac accaacagcc catgcagctt attttcaagg gcatccaggg
     1741 aagcaacacg gcctctgtgg ttgctatggg tttcatcttg atcaatcctg ggacttgtcc
     1801 agtaaaagtg ctaccagagc ttcctcccgt atctccagtt tcttccactg gcccttctga
     1861 aaccactggc ctcacagaaa accctacaat ctccaccaag aaacctacag tttccataga
     1921 aaaacccagt gtcaccacag aaaagcccac agtccccaaa gaaaagccca ccattcccac
     1981 agaaaaaccc accatctcca cagaaaaacc caccattcct tcagaaaaac ccaacatgcc
     2041 ctcagaaaaa cccaccattc cctcagaaaa acccaccatc ctcacagaaa aacccaccat
     2101 tccctcagaa aaacccacca ttccctcaga aaaacccacc atttccacag aaaaacccac
     2161 cgtccccaca gaagagccca ccacccccac tgaggagacc accacctcca tggaagagcc
     2221 tgtcatccct acagaaaaac ccagcatccc tacagaaaaa cccagcatcc ccacggaaaa
     2281 acccaccatc tccatggaag agactatcat ctccacagaa aaacccacca tctccccaga
     2341 aaaacccacc atccccacag aaaaacccac catccccaca gaaaaatcca ccatctcccc
     2401 agaaaaaccc accaccccca cagaaaaacc caccatcccc acagaaaaac ccaccatctc
     2461 cccagaaaaa cccaccaccc ccacagaaaa acccaccatc tccccagaaa aactcaccat
     2521 ccccacagaa aaacccacca tccccacaga aaaacccacc attcccacag aaaaacccac
     2581 catctccaca gaagagccca ccacccccac tgaggagacc accatctcca cagaaaaacc
     2641 cagcatcccc atggaaaaac ccactctccc cactgaagaa accaccacct ctgttgaaga
     2701 gactaccatc tctacagaaa aactcaccat ccccatggaa aaacccacca tctccacaga
     2761 aaaacccacc atccccacag aaaaacccac catctcccca gaaaaactca ccatccccac
     2821 ggaaaaactc accatcccca cggaaaaacc caccatcccc attgaagaga ctaccatctc
     2881 cacagaaaaa ctcaccatcc ccacagaaaa acccaccatc tccccagaaa aacccaccat
     2941 ctccacggaa aaacccacca tccccacgga aaaacccacc atccccactg aagagactac
     3001 catctccaca gaaaaactca ccatccccac agaaaaaccc accatctccc cagaaaaact
     3061 caccatcccc acagaaaaac ccaccatctc cacggaaaaa cccaccatcc ccacggaaaa
     3121 acttaccatc cccacggaaa aacccaccat ccccacagaa aaacccacca ttcccacaga
     3181 gaagctcaca gccctgaggc caccccatcc cagccccaca gccactgggc tggcagcctt
     3241 ggtgatgtct ccacatgctc caagtacccc tatgaccagt gtgattctgg gcactaccac
     3301 aacctccaga tccagtacag agcgctgccc tccaaatgcc cgctacgaat cctgtgcttg
     3361 tcctgcttcg tgcaagagcc ccaggcctag ctgtgggccc ctctgtcggg agggctgtgt
     3421 ctgcaaccct ggctttttgt ttagtgacaa ccactgcatc caggcctctt cctgcaattg
     3481 cttctacaac aacgactact atgagcctgg ggcagagtgg ttcagcccca actgcacaga
     3541 acattgccgc tgctggcccg gcagtcgggt cgagtgccag atctctcagt gtgggacaca
     3601 caccgtgtgc cagcttaaga atggccagta tggatgccac ccctacgcag gcactgccac
     3661 ctgcttggtc tacggagacc ctcattatgt cacctttgac gggaggcact ttggcttcat
     3721 gggcaagtgc acttacatct tggcccagcc ctgtggcaac tcaacagacc cattcttcag
     3781 ggtgacagcc aagaatgagg agcagggaca ggaaggcgtg tcctgcctga gcaaagtcta
     3841 cgtgaccctg cccgagagca ccgtcaccct gcttaagggc agacgcactc tggttggggg
     3901 tcagcaagtt actctcccag ccataccctc taaaggcgtc ttcctgggtg caagcgggcg
     3961 gtttgtggag ctgcagacgg agttcggttt gcgggtgaga tgggatggtg accagcagct
     4021 gtatgttact gtgtccagca catactctgg caaactctgt ggtttgtgtg ggaactatga
     4081 cggcaacagt gacaatgacc acctgaagtt ggacggcagc ccagcaggag acaaggagga
     4141 gctggggaac agctggcaga cggaccagga cgaggaccag gagtgtcaga agtaccaggt
     4201 ggtgaattcc ccgtcttgtg attcatctct gcagagcagc atgtcggggc cagggttctg
     4261 tggacggctg gtcgacactc atggcccatt tgagacatgc ctgctgcacg tgaaggccgc
     4321 ttccttcttc gacagctgca tgcttgatat gtgcggattc caggggctgc agcacctgct
     4381 gtgcacacac atgtccacca tgaccaccac ctgccaggac gcaggccacg ctgtgaagcc
     4441 ctggagggaa ccccacttct gcccaatggc ctgcccgccc aacagcaagt actccctgtg
     4501 tgcgaagcca tgccctgaca cctgccattc aggattctcc ggcatgttct gctcagaccg
     4561 gtgcgtggag gcctgtgaat gcaatccggg cttcgtcctc agtggcctcg agtgcatacc
     4621 tcgctcccag tgtgggtgcc tccaccctgc aggcagctac ttcaaggtag gggagcggtg
     4681 gtacaagcca ggctgcaaag agttgtgcgt ctgtgaaagc aacaacagaa ttcgctgcca
     4741 gccctggagg tgtagggccc aggagttctg tggccaacag gatggtatct atggctgcca
     4801 tgcccaaggt gccgccacct gcacagcctc gggtgacccc cactacctga ccttcgatgg
     4861 cgccttgcac cacttcatgg gcacctgcac ctatgtcctg acccggcctt gctggtccag
     4921 gtcccaagac agctattttg ttgtgagcgc caccaacgag aaccgcgggg ggatcctgga
     4981 ggtctcctac atcaaagccg tccacgtgac agtctttgac ctcagcatct cactgctcag
     5041 aggctgtaag gtcatgctga atggccatcg ggtggcccta cctgtgtggc ttgcacaagg
     5101 ccgggtgacc ataaggctca gcagcaacct cgtcctcctc tacacgaact ttgggctcca
     5161 agttcgctac gacgggagcc acttggtgga agtgacagtc ccctcctcct atggcggcca
     5221 gctctgtggg ctgtgtggga actacaacaa caacagcttg gatgacaacc tgcgccccga
     5281 cagaaagctt gcaggcgatt ccatgcagct gggggccgcc tggaagttac ctgaatcctc
     5341 tgaacctggc tgtttccttg tgggtggcaa gccctccagc tgccaggaga acagcatggc
     5401 agacgcctgg aacaagaact gtgcgatctt aataaaccct cagggaccct tctctcaatg
     5461 tcaccaggtg gtgcctcccc agtccagctt tgccagttgc gtgcatggtc agtgtgggac
     5521 caagggcgac accacagccc tgtgccgctc cctgcaggcc tacgcgtccc tgtgtgccca
     5581 ggctggccag gcccctgcct ggcggaacag aaccttctgc cctatgaggt gcccacctgg
     5641 cagcagctac agcccctgca gcagcccctg cccagacacc tgcagcagca taaacaaccc
     5701 gagggactgc cccaaagcac tgccctgtgc tgagagctgt gaatgtcaga aaggccacat
     5761 cttgagtgga acctcctgcg tgccccttgg ccagtgtggc tgcactgacc cagcgggctc
     5821 ctaccacccg gtcggggagc gctggtacac agagaacacc tgcaccaggc tctgcacctg
     5881 ctccgtccac aacaacatca cctgcttcca gagcacctgc aaacccaacc agatatgctg
     5941 ggccctggat gggctgctcc attgtcgggc ctcaggtgtg ggagtgtgtc agctcccagg
     6001 ggagtcccac tacgtgagct ttgatggtag taaccattct atcccggacg cctgcactct
     6061 tgtcctggtg aaagtgtgcc accccgccat ggccttgccc ttcttcaaga tcagtgccaa
     6121 gcatgagaag gaggaaggtg gaactgaggc tttccgcctt catgaggtct acattgacat
     6181 ctacgatgcc caggtcaccc tgcagaaggg ccaccgtgtg ctaatcaaca gcaaacaggt
     6241 caccctcccc gccatctccc agatccctgg ggtcagtgtc aagtccagca gcatctacag
     6301 cattgttaac atcaagatcg gggtgcaagt caagtttgac gggaatcatc tcttagagat
     6361 tgaaatcccc acaacctact atggaaaggt ctgcggcatg tgtgggaact tcaatgatga
     6421 ggaagaggac gaactaatga tgcccagcga tgaagtagca aatagtgaca gtgaatttgt
     6481 gaacagttgg aaagataagg acattgaccc aagttgtcag agtctcctgg tagatgagca
     6541 gcagattcca gcggaacagc aggagaaccc gagtggaaac tgcagggcgg ccgacctccg
     6601 cagggcgcgg gaaaagtgcg aggcagcgct ccgggctcct gtgtgggccc agtgcgcctc
     6661 ccgcatagac ctcacgccct tcctggtgga ctgtgcaaac accctctgtg agttcggagg
     6721 tctctaccag gccctctgcc aggctctgca agccttcggg gccacctgcc agagccaggg
     6781 gctcaagccc ccactctgga gaaacagcag cttctgccct ctggaatgcc ctgcctacag
     6841 cagctacacc aactgccttc cctcctgctc accctcctgc tgggacctgg atggccggtg
     6901 tgagggcgcc aaagtcccct ctgcctgcgc tgagggctgc atttgtcagc ccggctatgt
     6961 gctgagtgaa gacaagtgtg tccccagaag tcagtgtggc tgcaaggatg cccatggtgg
     7021 ctccatccct ctgggcaaga gctgggtctc cagcggttgc acggagaagt gtgtctgcac
     7081 gggaggagcc attcagtgcg gggacttccg atgcccctct gggtcccact gccagctcac
     7141 ttccgacaac agcaacagca attgtgtctc agacaagtct gaacaatgct cagtctatgg
     7201 cgacccccgt tacctcacat ttgacggctt cagctaccgc ttgcaaggcc gcatgaccta
     7261 tgttctgatc aagactgtgg acgtactgcc tgagggggtg gagcccctcc tcgtggaagg
     7321 acgcaacaag atggatccgc ccaggagctc catcttcttg caggaagtga ttaccaccgt
     7381 ctacggctat aaagtgcagc tccaagctgg tctggagctt gtggtcaaca accagaagat
     7441 ggccgtcccc tacaggccaa atgaacacct gcgggtcacc ctgtggggcc aacggctcta
     7501 cctggtcacc gactttgagc tggtcgtcag ctttggtgga aggaaaaatg cagtgatctc
     7561 cctacccagc atgtacgagg ggcttgtgag tggcctgtgc ggaaactacg acaagaaccg
     7621 caagaatgac atgatgctgc ccagtggcgc cctgacccag aacctcaaca cctttggcaa
     7681 cagctgggag gtgaagaccg aggacgcact cctgcgcttc cccagggcta taccagcgga
     7741 ggaggaggga caaggggcgg agctgggcct ccgcacgggc ctccaagtgt ccgaatgtag
     7801 cccggagcag ctggcgagca acagcaccca ggcctgtagg gtgctggcag acccccaggg
     7861 cccctttgct gcctgtcacc agacggtggc cccagagccc ttccaagagc actgcgtgct
     7921 ggatctgtgc tctgctcagg acccaagaga gcaagaggag ctgcgttgcc aggtcctcag
     7981 tggccatgga gtgtccagca ggtaccatat atcagagctg tatgacaccc tgcccagcat
     8041 cctgtgccaa cctggcagac cccggggact gcgagggccc ctgcgtggaa ggctgcgcca
     8101 gcatcccagg ctatgcctac agtggcaccc agagcctccc ctggctgact gtggctgcac
     8161 cagcaatggc atctactacc agctgggcag cagctttctg actgaggact gctctcagcg
     8221 gtgcacctgt gccagctcac ggatcctgct gtgtgagccc ttcagctgca gagcggggga
     8281 ggtctgcacc ctggggaacc acacccaagg ctgctttcca gaaagcccgt gtctgcagaa
     8341 cccctgtcag aatgacgggc agtgtcggga gcagggagcc accttcacct gcgagtgtga
     8401 agttggttac gggggaggcc tgtgtatgga gcctcgagat gcgccacctc ccagaaagcc
     8461 agcatctaac ctggtgggcg tcctactggg actgctggtg cctgtggtgg tcgtactact
     8521 ggccgtgacc agagagtgca tttacagaac gaggaggaag agagagaaaa cgcaggaggg
     8581 agacagactg gccaggctgg tggacacaga tactgttctg gactgtgcct gttaagttgc
     8641 tcagttttga gctgtcttca gacaagaaga ttaaataaat ttatatattt atttatttga
//
