LOCUS       NM_001282857           10079 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens 5'-3' exoribonuclease 1 (XRN1), transcript variant 3,
            mRNA.
ACCESSION   NM_001282857 XM_005247542
VERSION     NM_001282857.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   BenDavid,E., Pfaller,C.K., Pan,Y., Samuel,C.E. and Ma,D.
  TITLE     Host 5'-3' Exoribonuclease XRN1 Acts as a Proviral Factor for
            Measles Virus Replication by Downregulating the dsRNA-Activated
            Kinase PKR
  JOURNAL   J Virol 96 (22), e0131922 (2022)
   PUBMED   36300942
  REMARK    GeneRIF: Host 5'-3' Exoribonuclease XRN1 Acts as a Proviral Factor
            for Measles Virus Replication by Downregulating the dsRNA-Activated
            Kinase PKR.
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 10079)
  AUTHORS   Lekva,T., Roland,M.C.P., Estensen,M.E., Norwitz,E.R., Tilburgs,T.,
            Henriksen,T., Bollerslev,J., Normann,K.R., Magnus,P., Olstad,O.K.,
            Aukrust,P. and Ueland,T.
  TITLE     Dysregulated non-coding telomerase RNA component and associated
            exonuclease XRN1 in leucocytes from women developing
            preeclampsia-possible link to enhanced senescence
  JOURNAL   Sci Rep 11 (1), 19735 (2021)
   PUBMED   34611223
  REMARK    GeneRIF: Dysregulated non-coding telomerase RNA component and
            associated exonuclease XRN1 in leucocytes from women developing
            preeclampsia-possible link to enhanced senescence.
            Erratum:[Sci Rep. 2021 Nov 15;11(1):22572. doi:
            10.1038/s41598-021-00917-z.. PMID: 34782619]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10079)
  AUTHORS   Pashler,A.L., Towler,B.P., Jones,C.I., Haime,H.J., Burgess,T. and
            Newbury,S.F.
  TITLE     Genome-wide analyses of XRN1-sensitive targets in osteosarcoma
            cells identify disease-relevant transcripts containing G-rich
            motifs
  JOURNAL   RNA 27 (10), 1265-1280 (2021)
   PUBMED   34266995
  REMARK    GeneRIF: Genome-wide analyses of XRN1-sensitive targets in
            osteosarcoma cells identify disease-relevant transcripts containing
            G-rich motifs.
REFERENCE   5  (bases 1 to 10079)
  AUTHORS   Liu,Y.C., Mok,B.W., Wang,P., Kuo,R.L., Chen,H. and Shih,S.R.
  TITLE     Cellular 5'-3' mRNA Exoribonuclease XRN1 Inhibits Interferon Beta
            Activation and Facilitates Influenza A Virus Replication
  JOURNAL   mBio 12 (4), e0094521 (2021)
   PUBMED   34311580
  REMARK    GeneRIF: Cellular 5'-3' mRNA Exoribonuclease XRN1 Inhibits
            Interferon Beta Activation and Facilitates Influenza A Virus
            Replication.
REFERENCE   6  (bases 1 to 10079)
  AUTHORS   Lejeune,F., Li,X. and Maquat,L.E.
  TITLE     Nonsense-mediated mRNA decay in mammalian cells involves decapping,
            deadenylating, and exonucleolytic activities
  JOURNAL   Mol Cell 12 (3), 675-687 (2003)
   PUBMED   14527413
REFERENCE   7  (bases 1 to 10079)
  AUTHORS   Ingelfinger,D., Arndt-Jovin,D.J., Luhrmann,R. and Achsel,T.
  TITLE     The human LSm1-7 proteins colocalize with the mRNA-degrading
            enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci
  JOURNAL   RNA 8 (12), 1489-1501 (2002)
   PUBMED   12515382
  REMARK    GeneRIF: LSm1-7 proteins colocalize with DCP1,DCP2 and Xrn1 in
            cytoplasmic foci
REFERENCE   8  (bases 1 to 10079)
  AUTHORS   Zhang,K., Dion,N., Fuchs,B., Damron,T., Gitelis,S., Irwin,R.,
            O'Connor,M., Schwartz,H., Scully,S.P., Rock,M.G., Bolander,M.E. and
            Sarkar,G.
  TITLE     The human homolog of yeast SEP1 is a novel candidate tumor
            suppressor gene in osteogenic sarcoma
  JOURNAL   Gene 298 (2), 121-127 (2002)
   PUBMED   12426100
REFERENCE   9  (bases 1 to 10079)
  AUTHORS   Sato,Y., Shimamoto,A., Shobuike,T., Sugimoto,M., Ikeda,H.,
            Kuroda,S. and Furuichi,Y.
  TITLE     Cloning and characterization of human Sep1 (hSEP1) gene and
            cytoplasmic localization of its product
  JOURNAL   DNA Res 5 (4), 241-246 (1998)
   PUBMED   9802570
REFERENCE   10 (bases 1 to 10079)
  AUTHORS   Bashkirov,V.I., Scherthan,H., Solinger,J.A., Buerstedde,J.M. and
            Heyer,W.D.
  TITLE     A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4
            tetraplex substrates
  JOURNAL   J Cell Biol 136 (4), 761-773 (1997)
   PUBMED   9049243
  REMARK    GeneRIF: Mouse Xrn1 plays a role in RNA turnover.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK096177.1, AC109992.6 and
            AC023508.16.
            
            On Jun 2, 2019 this sequence version replaced NM_001282857.1.
            
            Summary: This gene encodes a member of the 5'-3' exonuclease
            family. The encoded protein may be involved in
            replication-dependent histone mRNA degradation, and interacts
            directly with the enhancer of mRNA-decapping protein 4. In addition
            to mRNA metabolism, a similar protein in yeast has been implicated
            in a variety of nuclear and cytoplasmic functions, including
            homologous recombination, meiosis, telomere maintenance, and
            microtubule assembly. Mutations in this gene are associated with
            osteosarcoma, suggesting that the encoded protein may also play a
            role in bone formation. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2013].
            
            Transcript Variant: This variant (3) lacks an in-frame exon in the
            3' coding region, compared to variant 1. The encoded isoform (c) is
            shorter, compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.1083392.1,
                                           SRR14038196.1377780.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392981.7/ ENSP00000376707.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AK096177.1         26-48
            24-168              AC109992.6         38881-39025         c
            169-401             AC109992.6         23672-23904         c
            402-499             AC109992.6         17755-17852         c
            500-609             AC109992.6         16440-16549         c
            610-720             AC109992.6         16233-16343         c
            721-803             AC109992.6         14571-14653         c
            804-891             AC109992.6         13846-13933         c
            892-1060            AC109992.6         13593-13761         c
            1061-1128           AC109992.6         12487-12554         c
            1129-1266           AC109992.6         12027-12164         c
            1267-1333           AC109992.6         9826-9892           c
            1334-1439           AC109992.6         9515-9620           c
            1440-1529           AC109992.6         8151-8240           c
            1530-1686           AC109992.6         5146-5302           c
            1687-1806           AC109992.6         3555-3674           c
            1807-1976           AC023508.16        130018-130187       c
            1977-2097           AC023508.16        128980-129100       c
            2098-2196           AC023508.16        128785-128883       c
            2197-2300           AC023508.16        125555-125658       c
            2301-2432           AC023508.16        122440-122571       c
            2433-2595           AC023508.16        109634-109796       c
            2596-2709           AC023508.16        108411-108524       c
            2710-2808           AC023508.16        105193-105291       c
            2809-2924           AC023508.16        101590-101705       c
            2925-3071           AC023508.16        100909-101055       c
            3072-3161           AC023508.16        96350-96439         c
            3162-3297           AC023508.16        95596-95731         c
            3298-3354           AC023508.16        90421-90477         c
            3355-3487           AC023508.16        90158-90290         c
            3488-3557           AC023508.16        84973-85042         c
            3558-3765           AC023508.16        82023-82230         c
            3766-3861           AC023508.16        80512-80607         c
            3862-3970           AC023508.16        72345-72453         c
            3971-4032           AC023508.16        60559-60620         c
            4033-4155           AC023508.16        58078-58200         c
            4156-4315           AC023508.16        57486-57645         c
            4316-4497           AC023508.16        54545-54726         c
            4498-4611           AC023508.16        43901-44014         c
            4612-4714           AC023508.16        43703-43805         c
            4715-4875           AC023508.16        37709-37869         c
            4876-10079          AC023508.16        31721-36924         c
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q23"
     gene            1..10079
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /note="5'-3' exoribonuclease 1"
                     /db_xref="GeneID:54464"
                     /db_xref="HGNC:HGNC:30654"
                     /db_xref="MIM:607994"
     exon            1..168
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..5178
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /note="isoform c is encoded by transcript variant 3;
                     strand-exchange protein 1 homolog"
                     /codon_start=1
                     /product="5'-3' exoribonuclease 1 isoform c"
                     /protein_id="NP_001269786.1"
                     /db_xref="CCDS:CCDS63801.1"
                     /db_xref="GeneID:54464"
                     /db_xref="HGNC:HGNC:30654"
                     /db_xref="MIM:607994"
                     /translation="MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCS
                     HPNDDDVHFRISDDKIFTDIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRF
                     RSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFMARLHEHLKYFVNMKISTDKS
                     WQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLIMLGLTSHE
                     AHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIE
                     RIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLP
                     RFEKYLVKLSDFDREHFSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKG
                     QENSLCWTALDKNEGEMITSKDNLEDETEDDDLFETEFRQYKRTYYMTKMGVDVVSDD
                     FLADQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLSDIHNISTLKIHFELGKPF
                     KPFEQLLAVLPAASKNLLPACYQHLMTNEDSPIIEYYPPDFKTDLNGKQQEWEAVVLI
                     PFIDEKRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFIYPSPWPEKFPAIER
                     CCTRYKIISLDAWRVDINKNKITRIDQKALYFCGFPTLKHIRHKFFLKKSGVQVFQQS
                     SRGENMMLEILVDAESDELTVENVASSVLGKSVFVNWPHLEEARVVAVSDGETKFYLE
                     EPPGTQKLYSGRTAPPSKVVHLGDKEQSNWAKEVQGISEHYLRRKGIIINETSAVVYA
                     QLLTGRKYQINQNGEVRLEKQWSKQVVPFVYQTIVKDIRAFDSRFSNIKTLDDLFPLR
                     SMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNLDALIQNQHKYSIKYNPG
                     YVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKK
                     VGSEWMYSSAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEI
                     ITWLKGHPVSTLSRSSCDLQILDAAIVEKIEEEVEKCKQRKNNKKVRVTVKPHLLYRP
                     LEQQHGVIPDRDAEFCLFDRVVNVRENFSVPVGLRGTIIGIKGANREADVLFEVLFDE
                     EFPGGLTIRCSPGRGYRLPTSALVNLSHGSRSETGNQKLTAIVKPQPAVHQHSSSSSV
                     SSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQGSGKMQYFQPTIQEKGAVLPQ
                     EISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSNKQPNSGIEN
                     FLASLNISKENEVQSSHHGEPPSEEHLSPQSFAMKGTRMLKEILKIDGSNTVDHKNEI
                     KQIANEIPVSSNRRDEYGLPSQPKQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQ
                     IPPVSTPVTELSRICSLVGMPQPDFSFLRMPQTMTVCQVKLSNGLLVHGPQCHSENEA
                     KEKAALFALQQLGSLGMNFPLPSQVFANYPSAVPPGTIPPAFPPPTANIMPSSSHLFG
                     SMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVHNQFIPLQVTKKRVANKKNFENKE
                     AQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQVETASQGHSISHH
                     KSTPISSSRRKSRKLAVNFGVSKPSE"
     exon            169..401
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            402..499
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            500..609
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            610..720
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            721..803
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            804..891
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1060
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1128
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1266
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1333
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1439
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1529
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1686
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1806
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1976
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2097
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2196
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2300
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2432
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2595
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2709
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2710..2808
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2809..2924
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2925..3071
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3072..3161
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3162..3297
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3298..3354
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3355..3487
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3488..3557
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3558..3765
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3766..3861
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3862..3970
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3971..4032
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4033..4155
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4156..4315
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4316..4497
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4498..4611
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4612..4714
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4715..4875
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4876..10079
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10056..10061
                     /regulatory_class="polyA_signal_sequence"
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /note="hexamer: ATTAAA"
     polyA_site      10079
                     /gene="XRN1"
                     /gene_synonym="SEP1"
                     /note="major polyA site"
ORIGIN      
        1 atccgcggag cgacgaccgg aggctgcggc ggagccccgg cggggcgttt ggtttcggtt
       61 tggccctgac tgggattagt gttgacgatc gaaatgggag tccccaagtt ttacagatgg
      121 atctcagagc ggtatccctg tctcagcgaa gtggtgaaag agcatcagat tcctgaattt
      181 gacaacttgt acctggatat gaatggaatt atacatcagt gctcccatcc taatgatgat
      241 gatgttcact ttagaatttc agatgataaa atctttactg atatttttca ctacctggag
      301 gtgttgtttc gcattattaa acccaggaaa gtgttcttta tggctgtaga tggtgtggct
      361 cctcgagcaa aaatgaacca gcagcgtggg aggcgtttta ggtcagcaaa ggaggcagaa
      421 gacaaaatta aaaaggcaat agagaaggga gaaactcttc ctacagaggc cagatttgat
      481 tccaactgta tcacaccagg aactgaattt atggccaggt tacatgaaca tctgaagtat
      541 tttgtaaata tgaaaatttc cacagacaag tcatggcaag gagttaccat ctacttctca
      601 ggccatgaga ctcctggaga aggagagcat aaaatcatgg aatttatcag atccgagaaa
      661 gcaaagccag atcatgatcc aaacaccaga cactgtcttt atggtttaga tgctgacttg
      721 attatgcttg gattaacaag tcatgaggca catttttctc tcttaagaga agaagttcga
      781 tttggtggca aaaaaacaca acgggtatgt gctccagaag aaactacatt tcaccttcta
      841 cacttgtctt taatgagaga gtatattgac tatgagtttt cagtattaaa agaaaagatc
      901 acatttaaat atgatattga aaggataata gatgattgga ttttgatggg gtttcttgtt
      961 ggtaatgatt ttatccctca tctacctcat ttacatatta atcatgatgc actgcctctt
     1021 ctttatggaa catatgttac catcctgcca gaacttgggg gttatattaa tgaaagtggg
     1081 cacctcaact tacctcgatt tgagaaatac cttgtgaaac tatcagattt tgatcgggag
     1141 cacttcagtg aagtttttgt ggacctaaaa tggtttgaaa gcaaagttgg taacaagtac
     1201 ctcaatgaag cagcaggtgt cgcagcagaa gaagccagga actacaagga aaagaaaaag
     1261 ttaaagggcc aggaaaattc tctgtgttgg actgctttag acaaaaatga aggcgaaatg
     1321 ataacttcta aggataattt agaagatgag actgaagatg atgacctatt tgaaactgag
     1381 tttagacaat ataaaagaac atattacatg acgaagatgg gggttgacgt agtatctgat
     1441 gactttctgg ctgatcaagc tgcatgttat gttcaggcaa tacagtggat tttgcactat
     1501 tactatcatg gagttcagtc ctggagctgg tattatcctt atcattatgc acctttcctg
     1561 tctgatatac acaacatcag tacactcaaa atccattttg aactaggaaa accttttaag
     1621 ccatttgaac agcttcttgc tgtacttcca gcagccagca aaaatttact tcctgcatgc
     1681 taccagcatt tgatgaccaa tgaagactca ccaattatag aatattaccc acctgatttt
     1741 aaaactgacc taaatgggaa acaacaggaa tgggaagctg tggtgttaat cccttttatt
     1801 gatgagaagc gattattgga agccatggag acatgtaacc actccctcaa aaaggaagag
     1861 aggaaaagaa accaacatag tgagtgccta atgtgctggt atgatagaga cacagagttt
     1921 atctatcctt ctccatggcc agaaaagttc cctgccatag aacgatgttg tacaaggtat
     1981 aaaataatat ccttagatgc ttggcgtgta gacataaaca aaaacaaaat aaccagaatt
     2041 gaccagaaag cattatattt ctgtggattt cctactctga aacacatcag acacaaattt
     2101 tttttgaaga aaagtggtgt tcaagtattc cagcaaagca gtcgtggaga aaacatgatg
     2161 ttggaaatct tagtggatgc agaatcagat gaacttaccg tagaaaatgt agcttcatca
     2221 gtgcttggaa aatctgtctt tgttaattgg cctcaccttg aggaagctag agtcgtggct
     2281 gtatcagatg gagaaactaa gttttacttg gaagaacctc caggaacaca gaagctttat
     2341 tcaggaagaa ctgccccacc atctaaagtg gttcatcttg gagataaaga acaatctaac
     2401 tgggcaaaag aagtacaagg aatttcagaa cactacctga gaagaaaagg aataataata
     2461 aatgaaacat ctgcagttgt gtatgctcag ttactcacag gtcgtaaata tcaaataaat
     2521 caaaatggtg aagttcgtct agagaaacag tggtcaaaac aagttgttcc ttttgtttat
     2581 caaactattg tcaaggacat ccgagctttc gactcccgtt tctccaatat caaaacattg
     2641 gatgatttgt ttcctctgag aagtatggtc tttatgctgg gaactcccta ttatggctgc
     2701 actggagaag ttcaggattc aggtgatgtg attacagaag gtaggattcg tgtgattttc
     2761 agcattccat gtgaacccaa tcttgatgct ttaatacaga accagcataa atattctata
     2821 aagtacaacc caggatatgt gttggccagt cgccttggag tgagtggata ccttgtttca
     2881 aggtttacag gaagtatttt tattggaaga ggatctagga gaaaccctca tggagaccat
     2941 aaagcaaatg tgggtttaaa tctcaaattc aacaagaaaa atgaggaggt acctggatat
     3001 actaagaaag ttggaagtga atggatgtat tcatctgcag cagaacaact tctggcagag
     3061 tacttagaga gagctccaga actatttagt tatatagcca aaaatagcca agaggatgtg
     3121 ttctatgaag atgacatttg gcctggagaa aatgagaatg gtgctgaaaa agttcaagaa
     3181 attattactt ggctaaaagg acatcctgtc agtactttat ctcgttcttc ttgtgattta
     3241 caaattctgg atgcagctat tgttgagaaa attgaggaag aagtcgaaaa gtgcaagcaa
     3301 agaaagaata ataagaaggt gcgagtaaca gtgaaacccc atttgctata cagaccttta
     3361 gaacagcaac atggagtcat tcctgatcgg gatgcagaat tttgtctttt tgaccgtgtt
     3421 gtaaatgtga gagaaaactt ctcagttcca gttggccttc gaggcaccat cataggaata
     3481 aaaggagcta atagagaagc cgatgtacta tttgaagtat tatttgatga agaatttcct
     3541 ggagggttaa caataagatg ctcacctggt agaggttatc gactgccaac aagtgccttg
     3601 gtgaaccttt ctcatgggag tcgctctgaa actggaaatc agaagttgac agccatcgta
     3661 aaaccacaac cagctgtaca tcaacatagc tcaagttcat cagtttcctc tgggcatttg
     3721 ggagccctca accattcccc tcaatcactt tttgttccta ctcaagtacc tactaaagat
     3781 gatgatgaat tctgcaacat ttggcagtcc ttacagggat ctggaaagat gcaatacttt
     3841 cagccaacta tacaagagaa gggtgcagtt ctacctcaag aaataagcca agtaaatcaa
     3901 catcataaat ctggctttaa tgacaacagt gttaaatatc agcaaagaaa acatgaccct
     3961 cacagaaaat ttaaagaaga gtgtaagagt cctaaagctg agtgttggtc ccaaaaaatg
     4021 tccaataagc agcctaactc tggaattgag aactttttag catctttgaa tatctccaaa
     4081 gaaaatgaag tacagtcatc tcatcatggg gagcctccaa gtgaagagca tttgtcacca
     4141 cagtcatttg ccatgaaggg aacacggatg cttaaagaaa ttctaaaaat tgatggctct
     4201 aacactgtgg accataagaa tgaaatcaaa cagattgcta atgaaatccc tgtttcctct
     4261 aacagaagag atgaatatgg attaccctct cagcctaaac aaaataagaa attagcatct
     4321 tatatgaaca agcctcacag tgctaatgag taccataatg ttcagtctat ggacaatatg
     4381 tgttggcctg cccccagcca gatccctcct gtatccacac cagtaactga actttctcga
     4441 atttgttccc ttgttggaat gccacaacct gatttctcct ttcttaggat gccacagaca
     4501 atgaccgttt gccaagtaaa attatctaat ggcttactgg tacatgggcc acagtgccac
     4561 tctgaaaatg aagccaaaga gaaagctgca ctttttgctt tacaacagtt gggctcctta
     4621 ggcatgaatt tccctttgcc ttcacaagta tttgcaaatt atccttcagc tgtaccacct
     4681 ggaaccattc ctccagcctt tcccccacct actgctaata taatgccttc gtcgtctcat
     4741 ctctttggct caatgccatg gggaccatcg gtgccagttc ctgggaagcc cttccatcat
     4801 actttatatt ctgggaccat gcccatggct gggggaatac cagggggtgt gcacaatcag
     4861 tttatacctc tgcaggttac taaaaaaagg gttgcaaaca aaaagaactt tgagaataag
     4921 gaagcccaga gttctcaagc cactccagtt cagactagcc agccagattc ttccaacatt
     4981 gtcaaagtaa gtccacggga gagctcatca gcttctttga agtcctctcc gattgctcaa
     5041 cctgcatctt cttttcaagt tgaaactgcc tctcaaggcc atagtatatc tcaccataag
     5101 tcaacaccaa tctcttcttc aagaagaaaa tcaagaaaac tggctgttaa ttttggtgtt
     5161 tctaaacctt ctgagtaaat ttggctctta gaattaagtt aatttcttct ctttccatct
     5221 acctttttat aaatacatat ctatgtctca taaaaattag aatgtactat tttaaaataa
     5281 tatgtgtaaa ttgaaatttt tttcattttt aagttatcag gcacttttca tgctgtttaa
     5341 aagactgtgt atcaaattgt gcactttaag tatgtgcagt ttgttgtatg tcaattatac
     5401 ctcaataaat ctgtaataaa aaactaaatt aaaccttgca ttaaaataat atcacagtat
     5461 cagtggacta aacattaaaa tgtaccactc taatcattgg cctcatgatt gaagcatcct
     5521 gaactatgaa ttagacatca gttagcaata ataagcattt tttacactat cattgaggaa
     5581 taattacatg gagcatgaaa tttgggcctc cagtataact tactgaatgt ggattttatt
     5641 tctcttttta atgatgtaag aaaattgtca ggagaatggc tcttatttat gtgtgtttta
     5701 acttatgctt tgttgcctct gagggtcttt agacctgctg tgaaaggatc acatttgttg
     5761 tggtgctgcc agttttgctt tattctaaat tttgtaccaa agcaacttta gaataatcag
     5821 aatatttcat ctaactgctt agaactataa atagcattct aaatttgagt aaatacaatt
     5881 ttttaggtta ctcaagaacc agcattagta atttctagta aaattgtttc aaaatctaca
     5941 gggtacaatg attacaaatt aatcttctaa caaaataaaa tatgaaatat tagtcatgtt
     6001 aattaataaa ctgtattatt ttgtatagct ttttatttgc ttacctgaca ttttatgaga
     6061 gcttcatagt tggtcggtat gtagtgcttc ttgggactga agaaattcta attgttgttt
     6121 aagttccaag gtgtgctaca atataggagg cacagtcatc agtttgtgac atacattatt
     6181 tactgcctat ttcattctga ttctatattt ttagctttat gctgaactgt tcaatgatgc
     6241 tgggattctc tctttgcatg tcatatgtga atgtgtggtt caagctgtaa ctgttgaaat
     6301 tattttagaa tttatgacga tttctcagca gggcctatgt ttatatatat atatatattt
     6361 attctttagt ttttgtgata tgcttatatt tttaaggaag taagttatag catacttttt
     6421 taaaagtagg aaccacaatc tttatatgga gcagcttgtt atatctgatt ttcatgtaac
     6481 tcattggaaa ctctgccatg actcttaaag tagtcttact tgttttttaa aatgctggta
     6541 ttaaactaaa tggactcctt tcactaactg ctgatttcag agtttaaatg ttagcaggat
     6601 atttgtgtat tagcagtgcc ttcagtataa gagagaaata ttcattatct gtcatttatc
     6661 atagtcgtat aagtttccat gattttattt tctgttattt tcataagttc agatgtttat
     6721 cagttcccaa cattgtttct ttatctgtag tgggatcttg agtaatgagg ctcaagtaaa
     6781 tcctcaatgt gtataattat tgcctcataa actctctctt tgtttttaaa ggaaacatct
     6841 attatggtaa tacaaagaaa agttaaattg tgacagtaat gttatattaa cctcttccta
     6901 ctttaaagtg tgaaatgttt cacctcagct gtacaaactc cagcattgag aatttgcctc
     6961 actcattact cctgtgtgac agttatataa atagcatgca gctcacatct gttttagagc
     7021 atagaagaag cggcacctgc catcttctga aaactccaaa gggaaatttc agtagacata
     7081 gtgcactaaa cccataagga tacttgacca atatttgagc acagcaagct gacagttcta
     7141 tacagatagg cagtgaaaga gttttatttt ccagccaggc gcagtggctc aaatcccagc
     7201 acttcgggag gctaaggcag gcggatcact tgaggtccgg agttccagac aagcctggcc
     7261 aacatgatga agccctgtct ctactaaaaa tacaaaaatt agccgggcgt gatggcacat
     7321 gcctttagtc ccagctactc gggaggctga ggcatgagaa tcatttgaac ccgcgaggca
     7381 gagtctgcag tgagccgaga ttgcaccact gcactccagc ctggagagca gagcaagact
     7441 ctgtctcaaa aaaaagaaaa aagttttatt atccttactt tttttttaac attattattc
     7501 taaaggtcaa aactgagaag agattaagat aggagagagc tccataatgg ctggatagtg
     7561 gtcaggcatt ctctattctt ttccccctgt agacccattc taaatgtggg cctgaggtca
     7621 atgggagatg tgccctccct atggaggatg taagaagcag aggccatttc tgccccatgt
     7681 tgaggaaaca atctgttgat agacctggaa tttagagtat atctgaaaag cagttggact
     7741 tcaagaaatt taaaattctc tctttgagat ggggtggact aagaccaccc ccaaagttta
     7801 aaaatatgct cattcaactt gaatcttctg aggacttttt gtgaaaatgg tggactgttt
     7861 agggcatagg acagattccc caaattgctt tatgcttcca cataactaga gcacttcaat
     7921 ctatttaagc ctttgtctcc taactgaaac attatttcta aatatttcta ttcaataagt
     7981 tttttctttt ttgagcaact taagtgaatt ttgaagattg ctccttccag tctcgttcct
     8041 tttcacattt tcctgcatta cctaataatt acctcagcgt tagaatttga atgtattaat
     8101 tgatttaaac atcatgtaga ctaaagtctt aaacatctaa gactcagttg tagttgcaga
     8161 aaaaaattaa agtcacctct actgaacctt ggtttcaatt taagattttt cttgcttttc
     8221 aaaggggtaa ctaataaaga ttgaagggtt tttttcccga ttggtggagg aatgaagaac
     8281 tattgatgca agttttttta ttgtttcttt ctatgatcag gtacctgctt tcattttaga
     8341 ctgctacttc caaactaagc tagattcttg gttttaatga gaggaggcag agagagggag
     8401 gcagatggaa gaagaaaaac agttaaataa gacataccca gtcactgcat ttttggacta
     8461 taccgtatct ttctagcaca aggaaataga taaactgtaa ggtctttttt actcactttt
     8521 tatttatgtg ttctatttgt gttagtcata actgtttata gtggttgtct aatttttact
     8581 tttattatat gagatagagc tgtgcagatc ttcactcaag ttagttctgt cacgcttctt
     8641 cccattattt agagcacagt tttttaaagc aactgtacaa tttctcagcc ttatgagctt
     8701 gctgtatttc ttggtatcat gttgcttcca aattttgttt ttactgtaga agacaattaa
     8761 tttattgtag agaagtggct gtgaaagtcg ttctctgtct ttaaaaatca attgcagaag
     8821 ttcatttgtc atttttctag agataaatta tagtactcaa actggcagtg ctcagtatat
     8881 acattttgta agctttgtca gtgaaaccat cagttttgca ggagcttcct ttcctagtca
     8941 agataaagct taaaattcca gaaattaatg tcgttcggac tgactttatt catatttcca
     9001 tcaaacttct ccaatacagt aagagatagt gttgaaccag catcaagtct ccagaaacat
     9061 ggcagagcag acaggccgtt aagtttcaca gacatcatag atccttttct taaagaagaa
     9121 aaacatgtat aaaatagttt tagtagtcca aaatgtcaac tatctgtagc tgcttttgtg
     9181 tgtgtgtcag tgaacaaata ataatgcctt gctcaatcaa tgcattcagc catctcaagt
     9241 gcaatttgtg aaggagacta tggtttccaa aagatacatt tttttacaaa gttaaacctg
     9301 taaaaagtct tttttttttt ccctccagcc gtacaccaac tgcacttggt tgtttcagca
     9361 gttggtatac tattaaattg tccaggccaa ataggtttct gtagctgttt tagtaatttg
     9421 aagccaaatt ctcatgctgt ttctcattaa aaagaatgag aatttggtcc atagttagct
     9481 ttaagttctc tcttcctttc tttcccttac agttaagggt ttggtggggg atggggaggt
     9541 tgttttcgtt ttttggattt ttttgtcttt tggctttaag tatcatattt tcttttgcct
     9601 gtatccaact gcttctttga gtattttcat ctagtttaat gtgagtaata gatgctgtgc
     9661 tgtcattgaa gtgttcaaca ttttgttcat ttaaacaaaa gtgtaattca tacatatata
     9721 gatacatatc ttaattgatt tctcaactat tttataagta actggaattt ttcattagat
     9781 cttatacaga gaagtatttt attaaaaatt caaaagggaa gacttttatg tgctcatttt
     9841 gtaatttttg attttaaata tctttacatt gtctgccaat taaagtgttt taaacttgca
     9901 ttggaatgga ctccgaatgt atttttgtgg tgttacgtta tccgtagatt tctagcatga
     9961 agttagcctc acgatgctgt gcaaaggatt ttaaaatatg agagtcactg aaagagttta
    10021 aacatctgtt catgttaaat gctctatgga ttttaattaa agacttgaga atgatttta
//
