LOCUS       NM_021141               3379 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens X-ray repair cross complementing 5 (XRCC5), mRNA.
ACCESSION   NM_021141
VERSION     NM_021141.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Xing,M., Xiong,Y. and Zhang,Y.
  TITLE     Ku80 is indispensable for repairing DNA double-strand breaks at
            highly methylated sites in human HCT116 cells
  JOURNAL   DNA Repair (Amst) 134, 103627 (2024)
   PUBMED   38219597
  REMARK    GeneRIF: Ku80 is indispensable for repairing DNA double-strand
            breaks at highly methylated sites in human HCT116 cells.
REFERENCE   3  (bases 1 to 3379)
  AUTHORS   Feng,D., He,J., Yuan,M., Chen,Q., Zeng,X., Zhou,Q., Wu,J. and
            Han,B.
  TITLE     SUMO2/3 promotes the progression and oxaliplatin resistance of
            colorectal cancer through facilitating the SUMOylation at Ku80-K307
  JOURNAL   Biofactors 49 (6), 1158-1173 (2023)
   PUBMED   37338025
  REMARK    GeneRIF: SUMO2/3 promotes the progression and oxaliplatin
            resistance of colorectal cancer through facilitating the
            SUMOylation at Ku80-K307.
REFERENCE   4  (bases 1 to 3379)
  AUTHORS   Ayoufu,A., Paierhati,P., Qiao,L., Zhang,N. and Abudukeremu,M.
  TITLE     RUSC1-AS1 promotes the malignant progression of breast cancer
            depending on the regulation of the miR-326/XRCC5 pathway
  JOURNAL   Thorac Cancer 14 (24), 2504-2514 (2023)
   PUBMED   37429610
  REMARK    GeneRIF: RUSC1-AS1 promotes the malignant progression of breast
            cancer depending on the regulation of the miR-326/XRCC5 pathway.
REFERENCE   5  (bases 1 to 3379)
  AUTHORS   Jakub,R., Filip,M., Daniel,B., Jolanta,H., Tomasz,H., Cezary,C.,
            Jan,L., Wenancjusz,D. and Domagala,P.
  TITLE     Nuclear expression of Ku70/80 is associated with CHEK2 germline
            mutations in breast cancer
  JOURNAL   Pol J Pathol 74 (2), 75-81 (2023)
   PUBMED   37728466
  REMARK    GeneRIF: Nuclear expression of Ku70/80 is associated with CHEK2
            germline mutations in breast cancer.
REFERENCE   6  (bases 1 to 3379)
  AUTHORS   Chen,D.J., Park,M.S., Campbell,E., Oshimura,M., Liu,P., Zhao,Y.,
            White,B.F. and Siciliano,M.J.
  TITLE     Assignment of a human DNA double-strand break repair gene (XRCC5)
            to chromosome 2
  JOURNAL   Genomics 13 (4), 1088-1094 (1992)
   PUBMED   1505945
REFERENCE   7  (bases 1 to 3379)
  AUTHORS   Jeggo,P.A., Hafezparast,M., Thompson,A.F., Broughton,B.C.,
            Kaur,G.P., Zdzienicka,M.Z. and Athwal,R.S.
  TITLE     Localization of a DNA repair gene (XRCC5) involved in
            double-strand-break rejoining to human chromosome 2
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6423-6427 (1992)
   PUBMED   1631138
REFERENCE   8  (bases 1 to 3379)
  AUTHORS   Wedrychowski,A., Henzel,W., Huston,L., Paslidis,N., Ellerson,D.,
            McRae,M., Seong,D., Howard,O.M. and Deisseroth,A.
  TITLE     Identification of proteins binding to interferon-inducible
            transcriptional enhancers in hematopoietic cells
  JOURNAL   J Biol Chem 267 (7), 4533-4540 (1992)
   PUBMED   1537839
REFERENCE   9  (bases 1 to 3379)
  AUTHORS   Knuth,M.W., Gunderson,S.I., Thompson,N.E., Strasheim,L.A. and
            Burgess,R.R.
  TITLE     Purification and characterization of proximal sequence
            element-binding protein 1, a transcription activating protein
            related to Ku and TREF that binds the proximal sequence element of
            the human U1 promoter
  JOURNAL   J Biol Chem 265 (29), 17911-17920 (1990)
   PUBMED   2211668
REFERENCE   10 (bases 1 to 3379)
  AUTHORS   Stuiver,M.H., Coenjaerts,F.E. and van der Vliet,P.C.
  TITLE     The autoantigen Ku is indistinguishable from NF IV, a protein
            forming multimeric protein-DNA complexes
  JOURNAL   J Exp Med 172 (4), 1049-1054 (1990)
   PUBMED   2212941
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP375944.1, BG717693.1,
            M30938.1, X57500.1 and BU184024.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_021141.3.
            
            Summary: The protein encoded by this gene is the 80-kilodalton
            subunit of the Ku heterodimer protein which is also known as
            ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is
            the DNA-binding component of the DNA-dependent protein kinase, and
            it functions together with the DNA ligase IV-XRCC4 complex in the
            repair of DNA double-strand break by non-homologous end joining and
            the completion of V(D)J recombination events.  This gene
            functionally complements Chinese hamster xrs-6, a mutant defective
            in DNA double-strand break repair and in ability to undergo V(D)J
            recombination. A rare microsatellite polymorphism in this gene is
            associated with cancer in patients of varying radiosensitivity.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.196403.1,
                                           SRR1660803.94913.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392132.7/ ENSP00000375977.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                BP375944.1         52-111
            61-63               BG717693.1         130-132
            64-3034             M30938.1           2-2972
            3035-3128           X57500.1           2635-2728
            3129-3366           M30938.1           3067-3304
            3367-3379           BU184024.1         554-566
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..3379
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /note="X-ray repair cross complementing 5"
                     /db_xref="GeneID:7520"
                     /db_xref="HGNC:HGNC:12833"
                     /db_xref="MIM:194364"
     exon            1..110
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..2288
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /EC_number="3.6.4.-"
                     /note="DNA repair protein XRCC5; Ku autoantigen, 80kDa;
                     Ku86 autoantigen related protein 1; ATP-dependent DNA
                     helicase 2 subunit 2; TLAA; CTC85; CTCBF; nuclear factor
                     IV; thyroid-lupus autoantigen; 86 kDa subunit of Ku
                     antigen; lupus Ku autoantigen protein p86; CTC box-binding
                     factor 85 kDa subunit; ATP-dependent DNA helicase II 80
                     kDa subunit; X-ray repair complementing defective repair
                     in Chinese hamster cells 5 (double-strand-break
                     rejoining)"
                     /codon_start=1
                     /product="X-ray repair cross-complementing protein 5"
                     /protein_id="NP_066964.1"
                     /db_xref="CCDS:CCDS2402.1"
                     /db_xref="GeneID:7520"
                     /db_xref="HGNC:HGNC:12833"
                     /db_xref="MIM:194364"
                     /translation="MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQR
                     QVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQPGS
                     QQADFLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKCDIS
                     LQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMISLEGE
                     DGLDEIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQERVKKTW
                     TVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQGFRYGSDIVPFSKVDEEQMKYK
                     SEGKCFSVLGFCKSSQVQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAI
                     VRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQ
                     LNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPI
                     QQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPTAKK
                     LKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFL
                     DTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDG
                     ITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI"
     exon            111..224
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            225..408
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            409..457
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            458..580
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            581..772
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            773..887
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1026
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1139
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1202
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1340
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1431
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1565
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1759
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1853
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1923
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2033
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2130
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2198
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..2273
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..3379
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2493..2498
                     /regulatory_class="polyA_signal_sequence"
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /note="hexamer: TATAAA"
     polyA_site      2510
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /note="major polyA site"
     regulatory      3347..3352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /note="hexamer: ATTAAA"
     regulatory      3355..3360
                     /regulatory_class="polyA_signal_sequence"
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /note="hexamer: CATAAA"
     polyA_site      3367
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
                     /note="major polyA site"
     polyA_site      3379
                     /gene="XRCC5"
                     /gene_synonym="KARP-1; KARP1; KU80; Ku86; KUB2; NFIV"
ORIGIN      
        1 gctatctgcc gcttgtccac cggaagcgag ttgcgacacg gcaggttccc gcccggaaga
       61 agcgaccaaa gcgcctgagg accggcaaca tggtgcggtc ggggaataag gcagctgttg
      121 tgctgtgtat ggacgtgggc tttaccatga gtaactccat tcctggtata gaatccccat
      181 ttgaacaagc aaagaaggtg ataaccatgt ttgtacagcg acaggtgttt gctgagaaca
      241 aggatgagat tgctttagtc ctgtttggta cagatggcac tgacaatccc ctttctggtg
      301 gggatcagta tcagaacatc acagtgcaca gacatctgat gctaccagat tttgatttgc
      361 tggaggacat tgaaagcaaa atccaaccag gttctcaaca ggctgacttc ctggatgcac
      421 taatcgtgag catggatgtg attcaacatg aaacaatagg aaagaagttt gagaagaggc
      481 atattgaaat attcactgac ctcagcagcc gattcagcaa aagtcagctg gatattataa
      541 ttcatagctt gaagaaatgt gacatctccc tgcaattctt cttgcctttc tcacttggca
      601 aggaagatgg aagtggggac agaggagatg gcccctttcg cttaggtggc catgggcctt
      661 cctttccact aaaaggaatt accgaacagc aaaaagaagg tcttgagata gtgaaaatgg
      721 tgatgatatc tttagaaggt gaagatgggt tggatgaaat ttattcattc agtgagagtc
      781 tgagaaaact gtgcgtcttc aagaaaattg agaggcattc cattcactgg ccctgccgac
      841 tgaccattgg ctccaatttg tctataagga ttgcagccta taaatcgatt ctacaggaga
      901 gagttaaaaa gacttggaca gttgtggatg caaaaaccct aaaaaaagaa gatatacaaa
      961 aagaaacagt ttattgctta aatgatgatg atgaaactga agttttaaaa gaggatatta
     1021 ttcaagggtt ccgctatgga agtgatatag ttcctttctc taaagtggat gaggaacaaa
     1081 tgaaatataa atcggagggg aagtgcttct ctgttttggg attttgtaaa tcttctcagg
     1141 ttcagagaag attcttcatg ggaaatcaag ttctaaaggt ctttgcagca agagatgatg
     1201 aggcagctgc agttgcactt tcctccctga ttcatgcttt ggatgactta gacatggtgg
     1261 ccatagttcg atatgcttat gacaaaagag ctaatcctca agtcggcgtg gcttttcctc
     1321 atatcaagca taactatgag tgtttagtgt atgtgcagct gcctttcatg gaagacttgc
     1381 ggcaatacat gttttcatcc ttgaaaaaca gtaagaaata tgctcccacc gaggcacagt
     1441 tgaatgctgt tgatgctttg attgactcca tgagcttggc aaagaaagat gagaagacag
     1501 acacccttga agacttgttt ccaaccacca aaatcccaaa tcctcgattt cagagattat
     1561 ttcagtgtct gctgcacaga gctttacatc cccgggagcc tctaccccca attcagcagc
     1621 atatttggaa tatgctgaat cctcccgctg aggtgacaac aaaaagtcag attcctctct
     1681 ctaaaataaa gacccttttt cctctgattg aagccaagaa aaaggatcaa gtgactgctc
     1741 aggaaatttt ccaagacaac catgaagatg gacctacagc taaaaaatta aagactgagc
     1801 aagggggagc ccacttcagc gtctccagtc tggctgaagg cagtgtcacc tctgttggaa
     1861 gtgtgaatcc tgctgaaaac ttccgtgttc tagtgaaaca gaagaaggcc agctttgagg
     1921 aagcgagtaa ccagctcata aatcacatcg aacagttttt ggatactaat gaaacaccgt
     1981 attttatgaa gagcatagac tgcatccgag ccttccggga agaagccatt aagttttcag
     2041 aagagcagcg ctttaacaac ttcctgaaag cccttcaaga gaaagtggaa attaaacaat
     2101 taaatcattt ctgggaaatt gttgtccagg atggaattac tctgatcacc aaagaggaag
     2161 cctctggaag ttctgtcaca gctgaggaag ccaaaaagtt tctggccccc aaagacaaac
     2221 caagtggaga cacagcagct gtatttgaag aaggtggtga tgtggacgat ttattggaca
     2281 tgatataggt cgtggatgta tggggaatct aagagagctg ccatcgctgt gatgctggga
     2341 gttctaacaa aacaagttgg atgcggccat tcaaggggag ccaaaatctc aagaaattcc
     2401 cagcaggtta cctggaggcg gatcatctaa ttctctgtgg aatgaataca cacatatata
     2461 ttacaaggga taatttagac cccatacaag tttataaaga gtcattgtta ttttctggtt
     2521 ggtgtattat tttttctgtg gtcttactga tctttgtata ttacatacat gctttgaagt
     2581 ttctggaaag tagatctttt cttgacctag tatatcagtg acagttgcag cccttgtgat
     2641 gtgattagtg tctcatgtgg aaccatggca tggttattga tgagtttctt aaccctttcc
     2701 agagtcctcc tttgcctgat cctccaacag ctgtcacaac ttgtgttgag caagcagtag
     2761 catttgcttc ctcccaacaa gcagctgggt taggaaaacc atgggtaagg acggactcac
     2821 ttctcttttt agttgaggcc ttctagttac cacattactc tgcctctgta tataggtggt
     2881 tttctttaag tggggtggga aggggagcac aatttccctt catactcctt ttaagcagtg
     2941 agttatggtg gtggtctcat gaagaaaaga ccttttggcc caatctctgc catatcagtg
     3001 aacctttaga aactcaaaaa ctgagaaatt tactacagta gttagaatta tatcacttca
     3061 ctgttctcta cttgcaagcc tcaaagagag aaagtttcgt tatattaaaa cacttaggta
     3121 acttttcggt ctttcccatt tctacctaag tcagctttca tctttgtgga tggtgtctcc
     3181 tttactaaat aagaaaataa caaagccctt attctctttt tttcttgtcc tcattcttgc
     3241 cttgagttcc agttcctctt tggtgtacag acttcttggt acccagtcac ctctgtcttc
     3301 agcaccctca taagtcgtca ctaatacaca gttttgtaca tgtaacatta aaggcataaa
     3361 tgactcatct ctctgtgaa
//
