LOCUS       NM_020135               3898 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens WRN helicase interacting protein 1 (WRNIP1),
            transcript variant 1, mRNA.
ACCESSION   NM_020135
VERSION     NM_020135.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Hegedus,L., Toth,A., Harami,G.M., Palinkas,J., Karatayeva,N.,
            Sajben-Nagy,E., Bene,S., Afzali Jaktajdinani,S., Kovacs,M.,
            Juhasz,S. and Burkovics,P.
  TITLE     Werner helicase interacting protein 1 contributes to G-quadruplex
            processing in human cells
  JOURNAL   Sci Rep 14 (1), 15740 (2024)
   PUBMED   38977862
  REMARK    GeneRIF: Werner helicase interacting protein 1 contributes to
            G-quadruplex processing in human cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Valenzisi,P., Marabitti,V., Pichierri,P. and Franchitto,A.
  TITLE     WRNIP1 prevents transcription-associated genomic instability
  JOURNAL   Elife 12, 89981 (2024)
   PUBMED   38488661
  REMARK    GeneRIF: WRNIP1 prevents transcription-associated genomic
            instability.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3898)
  AUTHORS   Marquez-Palencia,M., Reza Herrera,L., Parida,P.K., Ghosh,S.,
            Kim,K., Das,N.M., Gonzalez-Ericsson,P.I., Sanders,M.E.,
            Mobley,B.C., Diegeler,S., Aguilera,T.A., Peng,Y., Lewis,C.M.,
            Arteaga,C.L., Hanker,A.B., Whitehurst,A.W., Lorens,J.B.,
            Brekken,R.A., Davis,A.J. and Malladi,S.
  TITLE     AXL/WRNIP1 Mediates Replication Stress Response and Promotes
            Therapy Resistance and Metachronous Metastasis in HER2+ Breast
            Cancer
  JOURNAL   Cancer Res 84 (5), 675-687 (2024)
   PUBMED   38190717
  REMARK    GeneRIF: AXL/WRNIP1 Mediates Replication Stress Response and
            Promotes Therapy Resistance and Metachronous Metastasis in HER2+
            Breast Cancer.
REFERENCE   4  (bases 1 to 3898)
  AUTHORS   Jordan,B.R., Zhai,Y., Li,Z., Zhao,H., Mackmull,M.T. and Glavy,J.S.
  TITLE     Discovering the nuclear localization signal of Werner Helicase
            Interacting Protein 1
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1870 (7), 119502 (2023)
   PUBMED   37268023
  REMARK    GeneRIF: Discovering the nuclear localization signal of Werner
            Helicase Interacting Protein 1.
REFERENCE   5  (bases 1 to 3898)
  AUTHORS   Marabitti,V., Valenzisi,P., Lillo,G., Malacaria,E., Palermo,V.,
            Pichierri,P. and Franchitto,A.
  TITLE     R-Loop-Associated Genomic Instability and Implication of WRN and
            WRNIP1
  JOURNAL   Int J Mol Sci 23 (3), 1547 (2022)
   PUBMED   35163467
  REMARK    GeneRIF: R-Loop-Associated Genomic Instability and Implication of
            WRN and WRNIP1.
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3898)
  AUTHORS   Bish,R.A. and Myers,M.P.
  TITLE     Werner helicase-interacting protein 1 binds polyubiquitin via its
            zinc finger domain
  JOURNAL   J Biol Chem 282 (32), 23184-23193 (2007)
   PUBMED   17550899
  REMARK    GeneRIF: Werner helicase-interacting protein 1 binds polyubiquitin
            via its zinc finger domain
REFERENCE   7  (bases 1 to 3898)
  AUTHORS   Tsurimoto,T., Shinozaki,A., Yano,M., Seki,M. and Enomoto,T.
  TITLE     Human Werner helicase interacting protein 1 (WRNIP1) functions as a
            novel modulator for DNA polymerase delta
  JOURNAL   Genes Cells 10 (1), 13-22 (2005)
   PUBMED   15670210
  REMARK    GeneRIF: These results indicate that WRNIP1 functions as a
            modulator for initiation or restart events during pol
            delta-mediated DNA synthesis and that its ATPase activity is
            utilized to sense DNA ends and to regulate the extent of
            stimulation.
REFERENCE   8  (bases 1 to 3898)
  AUTHORS   Rush,J., Moritz,A., Lee,K.A., Guo,A., Goss,V.L., Spek,E.J.,
            Zhang,H., Zha,X.M., Polakiewicz,R.D. and Comb,M.J.
  TITLE     Immunoaffinity profiling of tyrosine phosphorylation in cancer
            cells
  JOURNAL   Nat Biotechnol 23 (1), 94-101 (2005)
   PUBMED   15592455
REFERENCE   9  (bases 1 to 3898)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   10 (bases 1 to 3898)
  AUTHORS   Kawabe,Yi., Branzei,D., Hayashi,T., Suzuki,H., Masuko,T., Onoda,F.,
            Heo,S.J., Ikeda,H., Shimamoto,A., Furuichi,Y., Seki,M. and
            Enomoto,T.
  TITLE     A novel protein interacts with the Werner's syndrome gene product
            physically and functionally
  JOURNAL   J Biol Chem 276 (23), 20364-20369 (2001)
   PUBMED   11301316
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL139092.12.
            
            On May 17, 2019 this sequence version replaced NM_020135.2.
            
            Summary: Werner's syndrome is a rare autosomal recessive disorder
            characterized by accelerated aging that is caused by defects in the
            Werner syndrome ATP-dependent helicase gene (WRN). The protein
            encoded by this gene interacts with the exonuclease-containing
            N-terminal portion of the Werner protein. This protein has a
            ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase
            domain, and two leucine zipper motifs in the C-terminus. It has
            sequence similarity to replication factor C family proteins and is
            conserved from E. coli to human. This protein likely accumulates at
            sites of DNA damage by interacting with polyubiquinated proteins
            and also binds to DNA polymerase delta and increases the initiation
            frequency of DNA polymerase delta-mediated DNA synthesis. This
            protein also interacts with nucleoporins at nuclear pore complexes.
            Two transcript variants encoding different isoforms have been
            isolated for this gene. [provided by RefSeq, Jul 2012].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF218313.2, AB056152.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380773.9/ ENSP00000370150.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1052              AL139092.12        69404-70455
            1053-1244           AL139092.12        72702-72893
            1245-1486           AL139092.12        74131-74372
            1487-1716           AL139092.12        83274-83503
            1717-1872           AL139092.12        87417-87572
            1873-1952           AL139092.12        88335-88414
            1953-3898           AL139092.12        89018-90963
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p25.2"
     gene            1..3898
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /note="WRN helicase interacting protein 1"
                     /db_xref="GeneID:56897"
                     /db_xref="HGNC:HGNC:20876"
                     /db_xref="MIM:608196"
     exon            1..1052
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /inference="alignment:Splign:2.1.0"
     CDS             231..2228
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /EC_number="3.6.1.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     putative helicase RUVBL; ATPase WRNIP1; Werner helicase
                     interacting protein 1"
                     /codon_start=1
                     /product="ATPase WRNIP1 isoform 1"
                     /protein_id="NP_064520.2"
                     /db_xref="CCDS:CCDS4475.1"
                     /db_xref="GeneID:56897"
                     /db_xref="HGNC:HGNC:20876"
                     /db_xref="MIM:608196"
                     /translation="MEVSGPEDDPFLSQLHQVQCPVCQQMMPAAHINSHLDRCLLLHP
                     AGHAEPAAGSHRAGERAKGPSPPGAKRRRLSESSALKQPATPTAAESSEGEGEEGDDG
                     GETESRESYDAPPTPSGARLIPDFPVARSSSPGRKGSGKRPAAAAAAGSASPRSWDEA
                     EAQEEEEAVGDGDGDGDADADGEDDPGHWDADAAEAATAFGASGGGRPHPRALAAEEI
                     RQMLQGKPLADTMRPDTLQDYFGQSKAVGQDTLLRSLLETNEIPSLILWGPPGCGKTT
                     LAHIIASNSKKHSIRFVTLSATNAKTNDVRDVIKQAQNEKSFFKRKTILFIDEIHRFN
                     KSQQDTFLPHVECGTITLIGATTENPSFQVNAALLSRCRVIVLEKLPVEAMVTILMRA
                     INSLGIHVLDSSRPTDPLSHSSNSSSEPAMFIEDKAVDTLAYLSDGDARAGLNGLQLA
                     VLARLSSRKMFCKKSGQSYSPSRVLITENDVKEGLQRSHILYDRAGEEHYNCISALHK
                     SMRGSDQNASLYWLARMLEGGEDPLYVARRLVRFASEDIGLADPSALTQAVAAYQGCH
                     FIGMPECEVLLAQCVVYFARAPKSIEVYSAYNNVKACLRNHQGPLPPVPLHLRNAPTR
                     LMKDLGYGKGYKYNPMYSEPVDQEYLPEELRGVDFFKQRRC"
     exon            1053..1244
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1486
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1716
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1872
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1952
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..3898
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2672..2677
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /note="hexamer: AATAAA"
     polyA_site      2691
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /note="major polyA site"
     regulatory      3843..3848
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
                     /note="hexamer: AATAAA"
     polyA_site      3865
                     /gene="WRNIP1"
                     /gene_synonym="bA420G6.2; CFAP93; FAP93; WHIP"
ORIGIN      
        1 acacttcccg acacgccgcg tgaggcgctg ccagcggccg gccgagggcg ggcggacgcg
       61 ggagctgcgg acgtgaggca tgagcggcgc cctcctccgg cccgcgagcg tcctgctggt
      121 tccccgagcg agggtctcgc ggcgcggggc ctagcggagg gcatcgaagg cctccgcgtg
      181 cgcacgggtt gctgcggccg cgccgggcgc cggggagggc ggcggccgcc atggaggtga
      241 gcgggccgga agacgacccc ttcctttcgc agctgcacca ggtgcagtgc cccgtgtgcc
      301 agcagatgat gcccgccgcg cacatcaact cgcacctgga ccgctgtctg ctgctccacc
      361 cggcggggca cgcggagccc gcggccgggt cgcaccgcgc cggggagcgg gccaaggggc
      421 cctcgccgcc cggcgccaag aggcggcggc tgtcggagag ctcggcgctg aagcagccag
      481 ccaccccgac ggcagccgag agcagcgagg gcgagggtga ggagggcgac gacggcggcg
      541 agaccgagag ccgcgagagc tacgacgcgc cgcccacacc cagcggcgcc cgccttatcc
      601 ccgacttccc ggtggcccgc tccagcagcc ccgggaggaa ggggtcgggg aagaggccgg
      661 cggccgccgc cgcggcgggg agcgcgtctc cgcgcagctg ggacgaggcg gaggcgcagg
      721 aggaggagga ggccgtgggc gacggcgatg gcgacgggga cgcggacgcg gacggcgagg
      781 acgacccggg gcactgggac gcggacgctg ccgaagccgc caccgccttc ggggccagtg
      841 gcgggggccg cccgcacccc cgggcgctgg ctgccgagga gatccgacag atgctacagg
      901 gcaagccgct ggccgacacg atgcgtcctg acacgctgca ggattacttc gggcagagca
      961 aggccgtggg ccaggatacc ctgctgcgct cgctcctgga gaccaacgaa atcccctcgc
     1021 ttatcctgtg ggggccgccg ggctgcggca agaccactct ggctcacatc atagccagca
     1081 acagcaagaa acatagcata aggtttgtga cattatctgc aacaaatgcc aagacaaatg
     1141 atgtgcgaga tgtcataaaa caagctcaaa atgaaaagag ctttttcaaa aggaaaacca
     1201 tcctttttat tgatgagatt catcggttca ataaatctca gcaggacact ttccttcctc
     1261 acgtggaatg tgggacgatc actctgattg gggcaaccac tgaaaaccct tccttccagg
     1321 tcaacgctgc tcttctgagc cgctgtcgag tgattgttct tgagaagctt ccagtagagg
     1381 caatggtgac tattttaatg cgagcgatca actccctggg aatccacgtc ctagactcta
     1441 gccgtcccac tgaccctctg agccacagca gcaacagcag ctcagagccc gccatgttca
     1501 tagaggataa agcagtagac accctggctt acctcagtga cggtgacgcc cgagctgggt
     1561 tgaacggact gcagctggcg gtgctggcta ggttaagctc taggaagatg ttctgtaaga
     1621 agagtgggca atcctattct cccagtagag ttctgatcac agagaatgac gtgaaggagg
     1681 gcctacagcg atcccacatt ttatatgacc gggcaggtga ggagcattac aactgcatct
     1741 ccgccctgca caagtccatg cggggctcag accagaacgc ctccctctac tggctggctc
     1801 gcatgctcga gggaggagag gacccactct acgtggcacg gaggcttgtc aggtttgcca
     1861 gcgaggacat aggtctggca gacccgtctg cgttaacaca agcggttgct gcctaccaag
     1921 gctgtcattt tataggcatg cctgaatgtg aggtgcttct ggcccagtgt gtggtctact
     1981 ttgccagagc cccaaagtcc attgaggtgt acagcgccta caacaacgtc aaagcctgcc
     2041 tgaggaacca ccaggggcca ctgccccccg tgcccctgca cctgaggaac gcgcccacta
     2101 ggctgatgaa ggatttgggc tatggcaaag gctacaagta caaccccatg tacagcgagc
     2161 ctgtggatca ggagtacctg cctgaagagt tgaggggggt agatttcttc aagcagagga
     2221 ggtgctgact cctcagggca cgacagcaga aggatgttgc ttttttaagg gagggccaga
     2281 aagaaagtta gtggattgca aagttggttg cctggtggaa gttagaacag accaacattt
     2341 tgtgccagaa atttaagagt tccataggtg gaggcgcagt tctttcgaat aaatgtgtaa
     2401 ctttgaaatt gtgttcattt gcactcggtg cagcggttat gcttatgaaa atacctggca
     2461 gctttgtgca atgaattaat gttataagga attatctatt ttgtcatagt atttaagtca
     2521 taatgtcatt tcagaattca gttctgtagg attttctttt ctttaaaaaa tgtatattct
     2581 gggtagtttt aattggtaaa aaaatgtaat tgtgatttaa tactgcatag tgttttgggt
     2641 atttttttta tatgcaaagg tcttacgagc caataaaact atttcaaagt actcttcgat
     2701 tctgtcatgg ttttcctgcc tggatgctag gtaccagcgt tgtcaccatt gcatttggtg
     2761 ggtggatact gggaaggaga aatcacccca gatgggaaga gtggggagct taagttaaga
     2821 agtcagtgtt atcttgttga aagttaactc tgatctcttt aaaggaatac ataaaggaat
     2881 tctttaaatg gcttgtggaa gactccagta gtcccatgcc cattttttcc cacttgtcct
     2941 ggtttcttct tgcagctcca tatttctaaa cagtcgtttt tcttttactt tatgtgtgtc
     3001 ctgaacacaa aatacgccac tccttctgct cagttaagag ttatttgtcc ctactgctac
     3061 ttcctctccc tctccttagt tgcatgtcgt gcatatgccc acaaggatgg ccccttcagg
     3121 tagtcggttc tcctcctgct gttgcgtgat ccctcctggg gtcctcctgc catgtctgga
     3181 aggctgccgg ctgttggcct gggacgtcct ctgcctttat tctgagtgag atgcccattt
     3241 tctggatgct ccatcccgcc ttactggttt attccgctat ttcagtgcaa cccatcctct
     3301 agtagtttta tcaggctgga gggactgtat gttgcagagg cttggcccga ctggcctccc
     3361 tccttctgtc tggagtggtt gcactgcagt gtgcagttgc catccccaga atctcccttc
     3421 atcatcaccc tggagctttc ctctgcttct ccctgcaccc catctgatga caggactgac
     3481 tgctttctgg cacaggtggc atgtaccctc tagtagcttt atggagcagg ggtgtgtgga
     3541 ggaaaagtat ttttagacct caggagactc aaaatgcttt tattgtacct tcagacgtgt
     3601 ctgagggttt ggctagtaat aaattgtagg taagaaatta cttgtgcatt gttttacact
     3661 ttcccacatt gttgtcaagt ttgaaggcat cctgatgtgt gtggtctttt gtttgtaacc
     3721 tctatcaact tgtagactct tctgtctttg ctccccgtgt tttaaaagtc tgcactgggt
     3781 acttgttggg cctttcaatc tggaaactcc tttcagttct agggaagttt ctttttttat
     3841 tcaataaatt tatttattta tgagaccaac tcttgctctt tttcccaggc tggagggc
//
