LOCUS       NM_001300939            1673 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens Wnt family member 8A (WNT8A), transcript variant 2,
            mRNA.
ACCESSION   NM_001300939 XM_005272076
VERSION     NM_001300939.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Nygaard,R., Yu,J., Kim,J., Ross,D.R., Parisi,G., Clarke,O.B.,
            Virshup,D.M. and Mancia,F.
  TITLE     Structural Basis of WLS/Evi-Mediated Wnt Transport and Secretion
  JOURNAL   Cell 184 (1), 194-206 (2021)
   PUBMED   33357447
  REMARK    GeneRIF: Structural Basis of WLS/Evi-Mediated Wnt Transport and
            Secretion.
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Mattes,B., Dang,Y., Greicius,G., Kaufmann,L.T., Prunsche,B.,
            Rosenbauer,J., Stegmaier,J., Mikut,R., Ozbek,S., Nienhaus,G.U.,
            Schug,A., Virshup,D.M. and Scholpp,S.
  TITLE     Wnt/PCP controls spreading of Wnt/beta-catenin signals by cytonemes
            in vertebrates
  JOURNAL   Elife 7, e36953 (2018)
   PUBMED   30060804
  REMARK    GeneRIF: Here, we show that Wnt/planar cell polarity (PCP)
            autocrine signaling controls the emergence of cytonemes, and that
            cytonemes subsequently control paracrine Wnt/beta-catenin signal
            activation. Upon binding of the Wnt family member Wnt8a, the
            receptor tyrosine kinase Ror2 becomes activated.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1673)
  AUTHORS   Voloshanenko,O., Gmach,P., Winter,J., Kranz,D. and Boutros,M.
  TITLE     Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting
            of receptor gene families
  JOURNAL   FASEB J 31 (11), 4832-4844 (2017)
   PUBMED   28733458
REFERENCE   4  (bases 1 to 1673)
  AUTHORS   Gao,H., Chen,D., Liu,X., Wu,M., Mi,J. and Wang,W.
  TITLE     Polymorphisms and expression of the WNT8A gene in Hirschsprung's
            disease
  JOURNAL   Int J Mol Med 32 (3), 647-652 (2013)
   PUBMED   23836442
  REMARK    GeneRIF: the WNT8A gene is involved in the susceptibility to HSCR,
            and plays an important role in the occurrence and development of
            Hirschsprung's disease.
REFERENCE   5  (bases 1 to 1673)
  AUTHORS   Ellinor,P.T., Lunetta,K.L., Albert,C.M., Glazer,N.L., Ritchie,M.D.,
            Smith,A.V., Arking,D.E., Muller-Nurasyid,M., Krijthe,B.P.,
            Lubitz,S.A., Bis,J.C., Chung,M.K., Dorr,M., Ozaki,K., Roberts,J.D.,
            Smith,J.G., Pfeufer,A., Sinner,M.F., Lohman,K., Ding,J.,
            Smith,N.L., Smith,J.D., Rienstra,M., Rice,K.M., Van Wagoner,D.R.,
            Magnani,J.W., Wakili,R., Clauss,S., Rotter,J.I., Steinbeck,G.,
            Launer,L.J., Davies,R.W., Borkovich,M., Harris,T.B., Lin,H.,
            Volker,U., Volzke,H., Milan,D.J., Hofman,A., Boerwinkle,E.,
            Chen,L.Y., Soliman,E.Z., Voight,B.F., Li,G., Chakravarti,A.,
            Kubo,M., Tedrow,U.B., Rose,L.M., Ridker,P.M., Conen,D., Tsunoda,T.,
            Furukawa,T., Sotoodehnia,N., Xu,S., Kamatani,N., Levy,D.,
            Nakamura,Y., Parvez,B., Mahida,S., Furie,K.L., Rosand,J.,
            Muhammad,R., Psaty,B.M., Meitinger,T., Perz,S., Wichmann,H.E.,
            Witteman,J.C., Kao,W.H., Kathiresan,S., Roden,D.M.,
            Uitterlinden,A.G., Rivadeneira,F., McKnight,B., Sjogren,M.,
            Newman,A.B., Liu,Y., Gollob,M.H., Melander,O., Tanaka,T.,
            Stricker,B.H., Felix,S.B., Alonso,A., Darbar,D., Barnard,J.,
            Chasman,D.I., Heckbert,S.R., Benjamin,E.J., Gudnason,V. and Kaab,S.
  TITLE     Meta-analysis identifies six new susceptibility loci for atrial
            fibrillation
  JOURNAL   Nat Genet 44 (6), 670-675 (2012)
   PUBMED   22544366
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1673)
  AUTHORS   Paige,S.L., Osugi,T., Afanasiev,O.K., Pabon,L., Reinecke,H. and
            Murry,C.E.
  TITLE     Endogenous Wnt/beta-catenin signaling is required for cardiac
            differentiation in human embryonic stem cells
  JOURNAL   PLoS One 5 (6), e11134 (2010)
   PUBMED   20559569
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1673)
  AUTHORS   Chiquet,B.T., Blanton,S.H., Burt,A., Ma,D., Stal,S., Mulliken,J.B.
            and Hecht,J.T.
  TITLE     Variation in WNT genes is associated with non-syndromic cleft lip
            with or without cleft palate
  JOURNAL   Hum Mol Genet 17 (14), 2212-2218 (2008)
   PUBMED   18413325
  REMARK    GeneRIF: Observational study of gene-disease association and
            gene-gene interaction. (HuGE Navigator)
REFERENCE   8  (bases 1 to 1673)
  AUTHORS   Saitoh,T., Mine,T. and Katoh,M.
  TITLE     Expression and regulation of WNT8A and WNT8B mRNAs in human tumor
            cell lines: up-regulation of WNT8B mRNA by beta-estradiol in MCF-7
            cells, and down-regulation of WNT8A and WNT8B mRNAs by retinoic
            acid in NT2 cells
  JOURNAL   Int J Oncol 20 (5), 999-1003 (2002)
   PUBMED   11956596
  REMARK    GeneRIF: Expression and regulation of WNT8A mRNA in human tumor
            cell lines
REFERENCE   9  (bases 1 to 1673)
  AUTHORS   Saitoh,T. and Katoh,M.
  TITLE     Molecular cloning and characterization of human WNT8A
  JOURNAL   Int J Oncol 19 (1), 123-127 (2001)
   PUBMED   11408932
REFERENCE   10 (bases 1 to 1673)
  AUTHORS   Smolich,B.D., McMahon,J.A., McMahon,A.P. and Papkoff,J.
  TITLE     Wnt family proteins are secreted and associated with the cell
            surface
  JOURNAL   Mol Biol Cell 4 (12), 1267-1275 (1993)
   PUBMED   8167409
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC144497.1, AB057725.1 and
            AY009402.1.
            
            On May 17, 2019 this sequence version replaced NM_001300939.1.
            
            Summary: The WNT gene family consists of structurally related genes
            which encode secreted signaling proteins. These proteins have been
            implicated in oncogenesis and in several developmental processes,
            including regulation of cell fate and patterning during
            embryogenesis. This gene is a member of the WNT gene family, and
            may be implicated in development of early embryos as well as germ
            cell tumors. Multiple alternatively spliced transcript variants
            have been found for this gene. [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (2) has an additional segment in
            the 3' region, which results in an alternate translation stop
            codon, compared to variant 1. The resulting isoform (2) is shorter
            and has a distinct C-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC144497.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000506684.6/ ENSP00000426653.1
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-526               BC144497.1         142-667
            527-889             BC144497.1         669-1031
            890-1642            AB057725.1         746-1498
            1643-1673           AY009402.1         1292-1322
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.2"
     gene            1..1673
                     /gene="WNT8A"
                     /gene_synonym="WNT8D"
                     /note="Wnt family member 8A"
                     /db_xref="GeneID:7478"
                     /db_xref="HGNC:HGNC:12788"
                     /db_xref="MIM:606360"
     exon            1..251
                     /gene="WNT8A"
                     /gene_synonym="WNT8D"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1205
                     /gene="WNT8A"
                     /gene_synonym="WNT8D"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; WNT8d; protein Wnt-8a; protein Wnt-8d;
                     wingless-type MMTV integration site family, member 8A"
                     /codon_start=1
                     /product="protein Wnt-8a isoform 2 precursor"
                     /protein_id="NP_001287868.1"
                     /db_xref="CCDS:CCDS75311.1"
                     /db_xref="GeneID:7478"
                     /db_xref="HGNC:HGNC:12788"
                     /db_xref="MIM:606360"
                     /translation="MLCCIQCLCLVSPFPTLTPCQGGPHCLIPIHLCLTFSLFGRSVN
                     NFLITGPKAYLTYTTSVALGAQSGIEECKFQFAWERWNCPENALQLSTHNRLRSATRE
                     TSFIHAISSAGVMYIITKNCSMGDFENCGCDGSNNGKTGGHGWIWGGCSDNVEFGERI
                     SKLFVDSLEKGKDARALMNLHNNRAGRLAVRATMKRTCKCHGISGSCSIQTCWLQLAE
                     FREMGDYLKAKYDQALKIEMDKRQLRAGNSAEGHWVPAEAFLPSAEAELIFLEESPDY
                     CTCNSSLGIYGTEGRECLQNSHNTSRWERRSCGRLCTECGLQVEERKTEVISSCNCKF
                     QWCCTVKCDQCRHVVSKYYCARSPGSAQSLGKGSA"
     exon            252..390
                     /gene="WNT8A"
                     /gene_synonym="WNT8D"
                     /inference="alignment:Splign:2.1.0"
     exon            391..516
                     /gene="WNT8A"
                     /gene_synonym="WNT8D"
                     /inference="alignment:Splign:2.1.0"
     exon            517..659
                     /gene="WNT8A"
                     /gene_synonym="WNT8D"
                     /inference="alignment:Splign:2.1.0"
     exon            660..1673
                     /gene="WNT8A"
                     /gene_synonym="WNT8D"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctcacttctc tggacttggc cctgagctgg acctggtcca ctggggtagg cagggcgatg
       61 gggaacctgt ttatgctctg ggcagctctg ggcatatgct gtgctgcatt cagtgcctct
      121 gcctggtaag tcctttccca accctcactc cttgccaagg aggcccccat tgtctcatcc
      181 ccattcacct ctgcctcact ttttctcttt ttggtaggtc agtgaacaat ttcctgataa
      241 caggtcccaa ggcctatctg acctacacga ctagtgtggc cttgggtgcc cagagtggca
      301 tcgaggagtg caagttccag tttgcttggg aacgctggaa ctgccctgaa aatgctcttc
      361 agctctccac ccacaacagg ctgagaagtg ctaccagaga gacttccttc atacatgcta
      421 tcagctctgc tggagtcatg tacatcatca ccaagaactg tagcatgggt gacttcgaaa
      481 actgtggctg tgatgggtca aacaatggaa aaacaggagg ccatggctgg atctggggag
      541 gctgcagcga caatgtggaa tttggggaaa ggatctccaa actctttgtg gacagtttgg
      601 agaaggggaa ggatgccaga gccctgatga atcttcacaa caacagggcc ggcagactgg
      661 cagtgagagc caccatgaaa aggacatgca aatgtcatgg catctctggg agctgcagca
      721 tacagacatg ctggctgcag ctggctgaat tccgggagat gggagactac ctaaaggcca
      781 agtatgacca ggcgctgaaa attgaaatgg ataagcggca gctgagagct gggaacagcg
      841 ccgagggcca ctgggtgccc gctgaggcct tccttcctag cgcagaggcg gaactgatct
      901 ttttagagga atcaccagat tactgtacct gcaattccag cctgggcatc tatggcacag
      961 agggtcgtga gtgcctacag aacagccaca acacatccag gtgggagcga cgtagctgtg
     1021 ggcgcctgtg cactgagtgt gggctgcagg tggaagagag gaaaactgag gtcataagca
     1081 gctgtaactg caaattccag tggtgctgta cggtcaagtg tgaccagtgt aggcatgtgg
     1141 tgagcaagta ttactgcgca cgctccccag gcagtgccca gtccctgggt aagggcagtg
     1201 cctgataata ccccacacaa gttcacttga ttaattgcat cagtggaagg ggacatagct
     1261 tctctcttag agagaacaga ttggaaagca atcggaaaat tgcagttttg gtctgtagtc
     1321 ctcatgatat ctgctatcag tggggaaaat ggaggcccaa gattctacag catattcctg
     1381 gcggggctga aattggaacc tgggcctcct gactttggca gacccccatt tcatctttcc
     1441 tgcaaactac tttcccatct ttgtgcctgt acttatgcag ctttctacag ggagagtttg
     1501 gtttggggtc tatatctaga gggaccttca aagtatttgt tcctttaaat ttcagaccat
     1561 gtccaaccca gctgtgctgc tgggaatcag gagaatagaa gcaaaaaacg aaagagttct
     1621 gttcagactt ctgaagagca gcctgtggct acaaatctat gctgataaat gag
//
