LOCUS       NM_032642               2238 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens Wnt family member 5B (WNT5B), transcript variant 1,
            mRNA.
ACCESSION   NM_032642
VERSION     NM_032642.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Zhao,H., Wu,G., Luo,Y., Xie,Y., Han,Y., Zhang,D., Han,Q., Zhao,X.,
            Qin,Y., Li,Q. and Wang,E.
  TITLE     WNT5B promotes the malignant phenotype of non-small cell lung
            cancer via the FZD3-DVL3-RAC1-PCP-JNK pathway
  JOURNAL   Cell Signal 122, 111330 (2024)
   PUBMED   39094673
  REMARK    GeneRIF: WNT5B promotes the malignant phenotype of non-small cell
            lung cancer via the FZD3-DVL3-RAC1-PCP-JNK pathway.
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Yamauchi,N., Otsuka,M., Ishikawa,T., Kakeji,Y., Kikuchi,A.,
            Masuda,A., Kodama,Y., Minami,Y. and Kamizaki,K.
  TITLE     Role of Wnt5b-Ror1 signaling in the proliferation of pancreatic
            ductal adenocarcinoma cells
  JOURNAL   Genes Cells 29 (6), 503-511 (2024)
   PUBMED   38531660
  REMARK    GeneRIF: Role of Wnt5b-Ror1 signaling in the proliferation of
            pancreatic ductal adenocarcinoma cells.
REFERENCE   3  (bases 1 to 2238)
  AUTHORS   Perkins,R.S., Murray,G., Suthon,S., Davis,L., Perkins,N.B. 3rd,
            Fletcher,L., Bozzi,A., Schreiber,S.L., Lin,J., Laxton,S.,
            Pillai,R.R., Wright,A.J., Miranda-Carboni,G.A. and Krum,S.A.
  TITLE     WNT5B drives osteosarcoma stemness, chemoresistance and metastasis
  JOURNAL   Clin Transl Med 14 (5), e1670 (2024)
   PUBMED   38689429
  REMARK    GeneRIF: WNT5B drives osteosarcoma stemness, chemoresistance and
            metastasis.
REFERENCE   4  (bases 1 to 2238)
  AUTHORS   Alve,S., Gramolelli,S., Jukonen,J., Juteau,S., Pink,A.,
            Manninen,A.A., Hanninen,S., Monto,E., Lackman,M.H., Carpen,O.,
            Saharinen,P., Karaman,S., Vaahtomeri,K. and Ojala,P.M.
  TITLE     DLL4/Notch3/WNT5B axis mediates bidirectional prometastatic
            crosstalk between melanoma and lymphatic endothelial cells
  JOURNAL   JCI Insight 9 (1), e171821 (2024)
   PUBMED   37971882
  REMARK    GeneRIF: DLL4/Notch3/WNT5B axis mediates bidirectional
            prometastatic crosstalk between melanoma and lymphatic endothelial
            cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2238)
  AUTHORS   Suthon,S., Lin,J., Perkins,R.S., Crockarell,J.R. Jr.,
            Miranda-Carboni,G.A. and Krum,S.A.
  TITLE     Estrogen receptor alpha and NFATc1 bind to a bone mineral
            density-associated SNP to repress WNT5B in osteoblasts
  JOURNAL   Am J Hum Genet 109 (1), 97-115 (2022)
   PUBMED   34906330
  REMARK    GeneRIF: Estrogen receptor alpha and NFATc1 bind to a bone mineral
            density-associated SNP to repress WNT5B in osteoblasts.
REFERENCE   6  (bases 1 to 2238)
  AUTHORS   Yates,K.E.
  TITLE     Demineralized bone alters expression of Wnt network components
            during chondroinduction of post-natal fibroblasts
  JOURNAL   Osteoarthritis Cartilage 12 (6), 497-505 (2004)
   PUBMED   15135146
REFERENCE   7  (bases 1 to 2238)
  AUTHORS   Saitoh,T. and Katoh,M.
  TITLE     Expression and regulation of WNT5A and WNT5B in human cancer:
            up-regulation of WNT5A by TNFalpha in MKN45 cells and up-regulation
            of WNT5B by beta-estradiol in MCF-7 cells
  JOURNAL   Int J Mol Med 10 (3), 345-349 (2002)
   PUBMED   12165812
  REMARK    GeneRIF: WNT5b is upregulated by beta-estradiol in tumor cell lines
REFERENCE   8  (bases 1 to 2238)
  AUTHORS   Saitoh,T. and Katoh,M.
  TITLE     Molecular cloning and characterization of human WNT5B on chromosome
            12p13.3 region
  JOURNAL   Int J Oncol 19 (2), 347-351 (2001)
   PUBMED   11445850
REFERENCE   9  (bases 1 to 2238)
  AUTHORS   Tanaka,K., Okabayashi,K., Asashima,M., Perrimon,N. and Kadowaki,T.
  TITLE     The evolutionarily conserved porcupine gene family is involved in
            the processing of the Wnt family
  JOURNAL   Eur J Biochem 267 (13), 4300-4311 (2000)
   PUBMED   10866835
REFERENCE   10 (bases 1 to 2238)
  AUTHORS   Smolich,B.D., McMahon,J.A., McMahon,A.P. and Papkoff,J.
  TITLE     Wnt family proteins are secreted and associated with the cell
            surface
  JOURNAL   Mol Biol Cell 4 (12), 1267-1275 (1993)
   PUBMED   8167409
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005182.2.
            
            On Nov 26, 2018 this sequence version replaced NM_032642.2.
            
            Summary: The WNT gene family consists of structurally related genes
            which encode secreted signaling proteins. These proteins have been
            implicated in oncogenesis and in several developmental processes,
            including regulation of cell fate and patterning during
            embryogenesis. This gene is a member of the WNT gene family. It
            encodes a protein which shows 94% and 80% amino acid identity to
            the mouse Wnt5b protein and the human WNT5A protein, respectively.
            Alternative splicing of this gene generates 2 transcript variants.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) differs from variant 2 in the
            5' UTR, which results from an alternate exon. Both variants encode
            the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC001749.1, SRR14038191.1749774.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397196.7/ ENSP00000380379.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               AC005182.2         47576-47716
            142-278             AC005182.2         49643-49779
            279-526             AC005182.2         51003-51250
            527-819             AC005182.2         58029-58321
            820-2238            AC005182.2         64139-65557
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.33"
     gene            1..2238
                     /gene="WNT5B"
                     /note="Wnt family member 5B"
                     /db_xref="GeneID:81029"
                     /db_xref="HGNC:HGNC:16265"
                     /db_xref="MIM:606361"
     exon            1..141
                     /gene="WNT5B"
                     /inference="alignment:Splign:2.1.0"
     exon            142..278
                     /gene="WNT5B"
                     /inference="alignment:Splign:2.1.0"
     CDS             199..1278
                     /gene="WNT5B"
                     /note="WNT-5B protein; protein Wnt-5b; wingless-type MMTV
                     integration site family, member 5B"
                     /codon_start=1
                     /product="protein Wnt-5b precursor"
                     /protein_id="NP_116031.1"
                     /db_xref="CCDS:CCDS8510.1"
                     /db_xref="GeneID:81029"
                     /db_xref="HGNC:HGNC:16265"
                     /db_xref="MIM:606361"
                     /translation="MPSLLLLFTAALLSSWAQLLTDANSWWSLALNPVQRPEMFIIGA
                     QPVCSQLPGLSPGQRKLCQLYQEHMAYIGEGAKTGIKECQHQFRQRRWNCSTADNASV
                     FGRVMQIGSRETAFTHAVSAAGVVNAISRACREGELSTCGCSRTARPKDLPRDWLWGG
                     CGDNVEYGYRFAKEFVDAREREKNFAKGSEEQGRVLMNLQNNEAGRRAVYKMADVACK
                     CHGVSGSCSLKTCWLQLAEFRKVGDRLKEKYDSAAAMRVTRKGRLELVNSRFTQPTPE
                     DLVYVDPSPDYCLRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYNQFKSVQVERC
                     HCKFHWCCFVRCKKCTEIVDQYICK"
     exon            279..526
                     /gene="WNT5B"
                     /inference="alignment:Splign:2.1.0"
     exon            527..819
                     /gene="WNT5B"
                     /inference="alignment:Splign:2.1.0"
     exon            820..2238
                     /gene="WNT5B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2220..2225
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WNT5B"
                     /note="hexamer: ATTAAA"
     polyA_site      2238
                     /gene="WNT5B"
                     /note="major polyA site"
ORIGIN      
        1 agtcacagcc acagtgacca ttagcaggca cccaggcctg tctttggctc ggaaacggtg
       61 gcccccaatg tagcctagtt tgaacctagg aactgcagga ccagagagat tccactggag
      121 cctgatggac gggtgacaga gggaacccta ctctggaaac tgtcagtccc agggcactgg
      181 ggagggctga ggccgaccat gcccagcctg ctgctgctgt tcacggctgc tctgctgtcc
      241 agctgggctc agcttctgac agacgccaac tcctggtggt cattagcttt gaacccggtg
      301 cagagacccg agatgtttat catcggtgcc cagcccgtgt gcagtcagct tcccgggctc
      361 tcccctggcc agaggaagct gtgccaattg taccaggagc acatggccta cataggggag
      421 ggagccaaga ctggcatcaa ggaatgccag caccagttcc ggcagcggcg gtggaattgc
      481 agcacagcgg acaacgcatc tgtctttggg agagtcatgc agataggcag ccgagagacc
      541 gccttcaccc acgcggtgag cgccgcgggc gtggtcaacg ccatcagccg ggcctgccgc
      601 gagggcgagc tctccacctg cggctgcagc cggacggcgc ggcccaagga cctgccccgg
      661 gactggctgt ggggcggctg tggggacaac gtggagtacg gctaccgctt cgccaaggag
      721 tttgtggatg cccgggagcg agagaagaac tttgccaaag gatcagagga gcagggccgg
      781 gtgctcatga acctgcaaaa caacgaggcc ggtcgcaggg ctgtgtataa gatggcagac
      841 gtagcctgca aatgccacgg cgtctcgggg tcctgcagcc tcaagacctg ctggctgcag
      901 ctggccgagt tccgcaaggt cggggaccgg ctgaaggaga agtacgacag cgcggccgcc
      961 atgcgcgtca cccgcaaggg ccggctggag ctggtcaaca gccgcttcac ccagcccacc
     1021 ccggaggacc tggtctatgt ggaccccagc cccgactact gcctgcgcaa cgagagcacg
     1081 ggctccctgg gcacgcaggg ccgcctctgc aacaagacct cggagggcat ggatggctgt
     1141 gagctcatgt gctgcgggcg tggctacaac cagttcaaga gcgtgcaggt ggagcgctgc
     1201 cactgcaagt tccactggtg ctgcttcgtc aggtgtaaga agtgcacgga gatcgtggac
     1261 cagtacatct gtaaatagcc cggagggcct gctcccggcc cccctgcact ctgcctcaca
     1321 aaggtctata ttatataaat ctatataaat ctattttata tttgtataag taaatgggtg
     1381 ggtgctatac aatggaaaga tgaaaatgga aaggaagagc ttatttaaga gacgctggag
     1441 atctctgagg agtggacttt gctggttctc tcctcttggt gggtgggaga cagggctttt
     1501 tctctccctc tggcgaggac tctcaggatg tagggacttg gaaatattta ctgtctgtcc
     1561 accacggcct ggaggaggga ggttgtggtt ggatggagga gatgatcttg tctggaagtc
     1621 tagagtcttt gttggttaga ggactgcctg tgatcctggc cactaggcca agaggcccta
     1681 tgaaggtggc gggaactcag cttcaacctc gatgtcttca gggtcttgtc cagaatgtag
     1741 atgggttccg taagaggcct ggtgctctct tactctttca tccacgtgca cttgtgcggc
     1801 atctgcagtt tacaggaacg gctccttccc taaaatgaga agtccaaggt catctctggc
     1861 ccagtgacca cagagagatc tgcacctccc ggacttcagg cctgcctttc cagcgagaat
     1921 tcttcatcct ccacggttca ctagctccta cctgaagagg aaagggggcc atttgacctg
     1981 acatgtcagg aaagccctaa actgaatgtt tgcgcctggg ctgcagaagc cagggtgcat
     2041 gaccaggctg cgtggacgtt atactgtctt cccccacccc cggggagggg aagcttgagc
     2101 tgctgctgtc actcctccac cgagggaggc ctcacaaacc acaggacgct gcaacgggtc
     2161 aggctggcgg gcccggcgtg ctcatcatct ctgccccagg tgtacggttt ctctctgaca
     2221 ttaaatgccc ttcatgga
//
