LOCUS       NM_032387               4199 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens WNK lysine deficient protein kinase 4 (WNK4),
            transcript variant 1, mRNA.
ACCESSION   NM_032387
VERSION     NM_032387.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Li,M., Shao,X., Ning,Q., Sun,R., Li,R., Liu,Y., Yuan,Y. and
            Zhang,Y.
  TITLE     Downregulation of WNK4 expression facilitates the proliferation of
            gastric cancer cells via activation of the STAT3 signaling pathway
  JOURNAL   Neoplasma 71 (3), 209-218 (2024)
   PUBMED   38764295
  REMARK    GeneRIF: Downregulation of WNK4 expression facilitates the
            proliferation of gastric cancer cells via activation of the STAT3
            signaling pathway.
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Wang,L., Wu,G. and Peng,J.B.
  TITLE     Identification of a novel KLHL3-interacting motif in the C-terminal
            region of WNK4
  JOURNAL   Biochem Biophys Res Commun 670, 87-93 (2023)
   PUBMED   37285722
  REMARK    GeneRIF: Identification of a novel KLHL3-interacting motif in the
            C-terminal region of WNK4.
REFERENCE   3  (bases 1 to 4199)
  AUTHORS   Ying,S., Guo,Q. and Zhang,C.
  TITLE     KLHL3-dependent WNK4 degradation affected by potassium through the
            neddylation and autophagy pathway
  JOURNAL   BMC Nephrol 24 (1), 217 (2023)
   PUBMED   37481568
  REMARK    GeneRIF: KLHL3-dependent WNK4 degradation affected by potassium
            through the neddylation and autophagy pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4199)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   5  (bases 1 to 4199)
  AUTHORS   Kahle,K.T., Gimenez,I., Hassan,H., Wilson,F.H., Wong,R.D.,
            Forbush,B., Aronson,P.S. and Lifton,R.P.
  TITLE     WNK4 regulates apical and basolateral Cl- flux in extrarenal
            epithelia
  JOURNAL   Proc Natl Acad Sci U S A 101 (7), 2064-2069 (2004)
   PUBMED   14769928
  REMARK    GeneRIF: general role for WNK4 in the regulation of electrolyte
            flux in diverse epithelia
REFERENCE   6  (bases 1 to 4199)
  AUTHORS   Wilson,F.H., Kahle,K.T., Sabath,E., Lalioti,M.D., Rapson,A.K.,
            Hoover,R.S., Hebert,S.C., Gamba,G. and Lifton,R.P.
  TITLE     Molecular pathogenesis of inherited hypertension with hyperkalemia:
            the Na-Cl cotransporter is inhibited by wild-type but not mutant
            WNK4
  JOURNAL   Proc Natl Acad Sci U S A 100 (2), 680-684 (2003)
   PUBMED   12515852
REFERENCE   7  (bases 1 to 4199)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Fariss,R.N., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of human RPE/choroid for the
            NEIBank Project: over 6000 non-redundant transcripts, novel genes
            and splice variants
  JOURNAL   Mol Vis 8, 205-220 (2002)
   PUBMED   12107410
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4199)
  AUTHORS   Verissimo,F. and Jordan,P.
  TITLE     WNK kinases, a novel protein kinase subfamily in multi-cellular
            organisms
  JOURNAL   Oncogene 20 (39), 5562-5569 (2001)
   PUBMED   11571656
REFERENCE   9  (bases 1 to 4199)
  AUTHORS   Wilson,F.H., Disse-Nicodeme,S., Choate,K.A., Ishikawa,K.,
            Nelson-Williams,C., Desitter,I., Gunel,M., Milford,D.V.,
            Lipkin,G.W., Achard,J.M., Feely,M.P., Dussol,B., Berland,Y.,
            Unwin,R.J., Mayan,H., Simon,D.B., Farfel,Z., Jeunemaitre,X. and
            Lifton,R.P.
  TITLE     Human hypertension caused by mutations in WNK kinases
  JOURNAL   Science 293 (5532), 1107-1112 (2001)
   PUBMED   11498583
REFERENCE   10 (bases 1 to 4199)
  AUTHORS   Ellison,D.H.
  TITLE     Pseudohypoaldosteronism Type II
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   22073419
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC100793.8, CA388932.1,
            BC136664.1 and BM683764.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_032387.4.
            
            Summary: This gene encodes a member of the WNK family of
            serine-threonine protein kinases. The kinase is part of the tight
            junction complex in kidney cells, and regulates the balance between
            NaCl reabsorption and K(+) secretion. The kinase regulates the
            activities of several types of ion channels, cotransporters, and
            exchangers involved in electrolyte flux in epithelial cells.
            Mutations in this gene result in pseudohypoaldosteronism type
            IIB.[provided by RefSeq, Sep 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC136664.1, AJ316534.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000246914.10/ ENSP00000246914.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AC100793.8         118383-118403
            22-71               CA388932.1         1-50
            72-4069             BC136664.1         1-3998
            4070-4199           BM683764.1         17-146              c
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.2"
     gene            1..4199
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /note="WNK lysine deficient protein kinase 4"
                     /db_xref="GeneID:65266"
                     /db_xref="HGNC:HGNC:14544"
                     /db_xref="MIM:601844"
     exon            1..707
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..3821
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein kinase WNK4; protein kinase with
                     no lysine 4; protein kinase lysine-deficient 4"
                     /codon_start=1
                     /product="serine/threonine-protein kinase WNK4 isoform 1"
                     /protein_id="NP_115763.2"
                     /db_xref="CCDS:CCDS11439.1"
                     /db_xref="GeneID:65266"
                     /db_xref="HGNC:HGNC:14544"
                     /db_xref="MIM:601844"
                     /translation="MLASPATETTVLMSQTEADLALRPPPPLGTAGQPRLGPPPRRAR
                     RFSGKAEPRPRSSRLSRRSSVDLGLLSSWSLPASPAPDPPDPPDSAGPGPARSPPPSS
                     KEPPEGTWTEGAPVKAAEDSARPELPDSAVGPGSREPLRVPEAVALERRREQEEKEDM
                     ETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRF
                     SEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRV
                     LQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPTGSVKIGDLGLATLKRASFAK
                     SVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGR
                     KPNSFHKVKIPEVKEIIEGCIRTDKNERFTIQDLLAHAFFREERGVHVELAEEDDGEK
                     PGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVAR
                     AVRERVAAIQRKREKLRKARELEALPPEPGPPPATVPMAPGPPSVFPPEPEEPEADQH
                     QPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGVPSSLAESHLCLPSAFA
                     LSIPRSGPGSDFSPGDSYASDAASGLSDVGEGMGQMRRPPGRNLRRRPRSRLRVTSVS
                     DQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLRRIREI
                     IQRVETLLKRDTGPMEAAEDTLSPQEEPAPLPALPVPLPDPSNEELQSSTSLEHRSWT
                     AFSTSSSSPGTPLSPGNPFSPGTPISPGPIFPITSPPCHPSPSPFSPISSQVSSNPSP
                     HPTSSPLPFSSSTPEFPVPLSQCPWSSLPTTSPPTFSPTCSQVTLSSPFFPPCPSTSS
                     FPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPSPLPSLPLPPPVAPG
                     GQESPSPHTAEVESEASPPPARPLPGEARLAPISEEGKPQLVGRFQVTSSKEPAEPLP
                     LQPTSPTLSGSPKPSTPQLTSESSDTEDSAGGGPETREALAESDRAAEGLGAGVEEEG
                     DDGKEPQVGGSPQPLSHPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQSLRQKHL
                     SEVETLQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQR
                     SEPPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM"
     exon            708..880
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1101
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1259
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1348
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1565
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1830
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1831..1952
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2011
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2129
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2246
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2384
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2385..2439
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..3050
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3111
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3112..3520
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3521..3720
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3721..3818
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     exon            3819..4199
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4177..4182
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /note="hexamer: AATAAA"
     polyA_site      4199
                     /gene="WNK4"
                     /gene_synonym="PHA2B; PRKWNK4"
                     /note="major polyA site"
ORIGIN      
        1 ggagcgcagc gcacccagcg agtccgtctg tcaggccgcc tcctctccgg ccgtctgatt
       61 ttctaccctt cggcgccctg ctcttcctca tgttggcatc cccggccacg gagaccaccg
      121 tcctcatgtc ccagactgag gccgacctgg ccctgcggcc cccgcctcct cttggcaccg
      181 cggggcagcc ccgcctcggg ccccctcctc gccgagcgcg ccgcttctcc gggaaggctg
      241 agccccggcc gcgctcttct cgtctcagcc gccgtagctc agtcgacttg gggctgctga
      301 gctcttggtc cctgccagcc tcacccgctc cggacccccc cgatcctccg gactccgctg
      361 gtcctggccc cgcgaggagc ccaccgccta gctccaaaga accccccgag ggcacgtgga
      421 ccgagggagc ccctgtgaag gctgcggaag actccgcgcg tcccgagctc ccggactctg
      481 cagtgggccc ggggtccagg gagccgctaa gggtccctga agctgtggcc ctagagcggc
      541 ggcgggagca ggaagaaaag gaggacatgg agacccaggc tgtggcaacg tcccccgatg
      601 gccgatacct caagtttgac atcgagattg gacgtggctc cttcaagacg gtgtatcgag
      661 ggctagacac cgacaccaca gtggaggtgg cctggtgtga gctgcagact cggaaactgt
      721 ctagagctga gcggcagcgc ttctcagagg aggtggagat gctcaagggg ctgcagcacc
      781 ccaacatcgt ccgcttctat gattcgtgga agtcggtgct gaggggccag gtttgcatcg
      841 tgctggtcac cgaactcatg acctcgggca cgctcaagac gtacctgagg cggttccggg
      901 agatgaagcc gcgggtcctt cagcgctgga gccgccaaat cctgcgggga cttcatttcc
      961 tacactcccg ggttcctccc atcctgcacc gggatctcaa gtgcgacaat gtctttatca
     1021 cgggacctac tggctctgtc aaaatcgggg acctgggcct ggccacgctc aagcgcgcct
     1081 cctttgccaa gagtgtcatc gggaccccgg aattcatggc ccccgagatg tacgaggaaa
     1141 agtacgatga ggccgtggac gtgtacgcgt tcggcatgtg catgctggag atggccacct
     1201 ctgagtaccc gtactccgag tgccagaatg ccgcgcaaat ctaccgcaag gtcacttcgg
     1261 gcagaaagcc gaacagcttc cacaaggtga agatacccga ggtgaaggag atcattgaag
     1321 gctgcatccg cacggataag aacgagaggt tcaccatcca ggacctcctg gcccacgcct
     1381 tcttccgcga ggagcgcggt gtgcacgtgg aactagcgga ggaggacgac ggcgagaagc
     1441 cgggcctcaa gctctggctg cgcatggagg acgcgcggcg cggggggcgc ccacgggaca
     1501 accaggccat cgagttcctg ttccagctgg gccgggacgc ggccgaggag gtggcacagg
     1561 agatggtggc tctgggcttg gtctgtgaag ccgattacca gccagtggcc cgtgcagtac
     1621 gtgaacgggt tgctgccatc cagcgaaagc gtgagaagct gcgtaaagca agggaattgg
     1681 aggcactccc accagagcca ggacctccac cagcaactgt gcccatggcc cccggtcccc
     1741 ccagtgtctt cccccctgag cctgaggagc cagaggcaga ccagcaccag cccttccttt
     1801 tccgccacgc cagctactca tctaccactt cggattgcga gactgatggc tacctcagct
     1861 cctccggctt cctggatgcc tcagaccctg cccttcagcc ccctgggggg gtgccatcca
     1921 gcctggctga gtcccatctc tgcctgccct cggcttttgc cctatccatt ccacgttctg
     1981 gccctggaag tgacttttcc cccggggaca gctatgcctc agatgcagct tcaggcctta
     2041 gcgatgtggg agaagggatg ggacaaatga ggagaccccc agggaggaat ctccggcgca
     2101 gaccccgatc ccggctgcgg gtcactagtg tctcagacca gaatgacaga gtggttgagt
     2161 gccagctaca gacccataac agcaagatgg tgaccttccg atttgatctg gatggggaca
     2221 gcccggaaga gattgcagct gccatggtat ataacgagtt cattctgcct tcggagcgag
     2281 atggatttct cagacggatt cgggagatta tccagcgagt ggagaccctg ttgaagagag
     2341 acactggccc catggaggct gctgaagaca ccctaagccc ccaggaggag ccagcaccat
     2401 tacctgccct gcccgtcccc ctcccagacc catccaatga agagctccag agcagcacct
     2461 ccctggagca caggagctgg acagccttct ccacctcctc atcttctcct ggaactcctt
     2521 tgtctcctgg aaacccattt tcccctggaa cccccatttc cccaggtccc atcttcccca
     2581 tcacttctcc cccatgtcat cccagcccct ccccattctc ccccatttct tcccaggtct
     2641 cctcaaatcc ctctccacac cccaccagct ctccacttcc attctcctcc agcacacccg
     2701 agtttccggt cccactctct cagtgtccct ggagttctct ccccacgact tctccaccta
     2761 cgttctctcc cacttgttct caggtcactc ttagttcccc tttctttcct ccgtgcccct
     2821 ccacttcttc cttcccctcc accacagcag cccctctcct ttctctggct agtgccttct
     2881 cactggctgt gatgactgtg gcccagtccc tgctgtcccc ctcacctggg ctcctttccc
     2941 agtctcctcc agcccctcct agtcccctcc ctagcctgcc ccttccccct cccgttgctc
     3001 ctggtggcca ggaaagccct tcaccccaca cagctgaggt ggagagtgag gcctcaccac
     3061 ctcctgctcg gcccctccca ggggaagcca ggctggcgcc catctctgaa gagggaaagc
     3121 cgcagcttgt tgggcgtttc caagtgactt catccaagga accggctgag cctcttccct
     3181 tgcagccaac atcccccact ctctctggtt ctccaaaacc ttcaacccct cagctcactt
     3241 cagagagctc agatacagag gacagtgctg gaggcgggcc agagaccagg gaagctctgg
     3301 ctgagagcga ccgtgcagct gagggtctgg gggctggagt tgaggaggaa ggagatgatg
     3361 ggaaggaacc ccaagttggg ggcagccccc aacccctgag ccatcccagc ccagtgtgga
     3421 tgaactactc ctacagcagc ctgtgtttga gcagcgagga gtcagaaagc agtggggaag
     3481 atgaggagtt ctgggctgag ctgcagagtc ttcggcagaa gcacttgtca gaggtggaaa
     3541 cactacagac actacagaaa aaagaaattg aagatttgta cagccggctg gggaagcagc
     3601 ccccaccggg tattgtggcc ccagctgcta tgctgtccag ccgccagcgc cgcctctcca
     3661 agggcagctt ccccacctcc cgccgcaaca gcctacagcg ctctgagccc ccaggccctg
     3721 gcatcatgcg aaggaactct ctgagtggca gcagcaccgg ctcccaggag cagcgggcaa
     3781 gcaagggggt gacattcgcc ggggatgttg gcaggatgtg aattcagaac agaagccatg
     3841 tatctccccc acaccagggc ccaccatgga gcttgtgttc tcagaatctg atgctttctg
     3901 atcaacaaaa ctgagcaagg aagatcccaa cactgaaggg gtagaaggcc aggggggcat
     3961 ggagagtgca gctccattat agtgaagagc caaacatatg tgaactgttt gctgtgtgga
     4021 ggtgttagtt ctgctgccta ccatcttcat ctctagcacc tcccctgcca agagtcaacc
     4081 actaagcaat cccacccaag cctggatgct tctagagggg cccactccca gctgggagag
     4141 tgtaggggat atgctcacac cacattagca gcaaccaata aaaatgctgg aaacaagaa
//
