LOCUS       NM_020922              11384 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens WNK lysine deficient protein kinase 3 (WNK3),
            transcript variant 1, mRNA.
ACCESSION   NM_020922
VERSION     NM_020922.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Boyd-Shiwarski,C.R., Shiwarski,D.J., Griffiths,S.E., Beacham,R.T.,
            Norrell,L., Morrison,D.E., Wang,J., Mann,J., Tennant,W.,
            Anderson,E.N., Franks,J., Calderon,M., Connolly,K.A., Cheema,M.U.,
            Weaver,C.J., Nkashama,L.J., Weckerly,C.C., Querry,K.E.,
            Pandey,U.B., Donnelly,C.J., Sun,D., Rodan,A.R. and Subramanya,A.R.
  TITLE     WNK kinases sense molecular crowding and rescue cell volume via
            phase separation
  JOURNAL   Cell 185 (24), 4488-4506 (2022)
   PUBMED   36318922
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   Yoon,H.J., Kim,G.C., Oh,S., Kim,H., Kim,Y.K., Lee,Y., Kim,M.S.,
            Kwon,G., Ok,Y.S., Kwon,H.K. and Kim,H.S.
  TITLE     WNK3 inhibition elicits antitumor immunity by suppressing PD-L1
            expression on tumor cells and activating T-cell function
  JOURNAL   Exp Mol Med 54 (11), 1913-1926 (2022)
   PUBMED   36357569
  REMARK    GeneRIF: WNK3 inhibition elicits antitumor immunity by suppressing
            PD-L1 expression on tumor cells and activating T-cell function.
REFERENCE   3  (bases 1 to 11384)
  AUTHORS   Pleinis,J.M., Norrell,L., Akella,R., Humphreys,J.M., He,H., Sun,Q.,
            Zhang,F., Sosa-Pagan,J., Morrison,D.E., Schellinger,J.N.,
            Jackson,L.K., Goldsmith,E.J. and Rodan,A.R.
  TITLE     WNKs are potassium-sensitive kinases
  JOURNAL   Am J Physiol Cell Physiol 320 (5), C703-C721 (2021)
   PUBMED   33439774
  REMARK    GeneRIF: WNKs are potassium-sensitive kinases.
REFERENCE   4  (bases 1 to 11384)
  AUTHORS   Gong,Y.T., Wu,M.Y., Tang,J.F., Shen,J.C., Li,J., Gao,R., Dang,B.Q.
            and Chen,G.
  TITLE     Advances in the study of the role and molecular mechanism of
            with-no-lysine kinase 3 in nervous system diseases (Review)
  JOURNAL   Mol Med Rep 23 (5) (2021)
   PUBMED   33760209
  REMARK    GeneRIF: Advances in the study of the role and molecular mechanism
            of withnolysine kinase 3 in nervous system diseases (Review).
            Review article
REFERENCE   5  (bases 1 to 11384)
  AUTHORS   Akella,R., Drozdz,M.A., Humphreys,J.M., Jiou,J., Durbacz,M.Z.,
            Mohammed,Z.J., He,H., Liwocha,J., Sekulski,K. and Goldsmith,E.J.
  TITLE     A Phosphorylated Intermediate in the Activation of WNK Kinases
  JOURNAL   Biochemistry 59 (18), 1747-1755 (2020)
   PUBMED   32314908
  REMARK    GeneRIF: A Phosphorylated Intermediate in the Activation of WNK
            Kinases.
REFERENCE   6  (bases 1 to 11384)
  AUTHORS   Holden,S., Cox,J. and Raymond,F.L.
  TITLE     Cloning, genomic organization, alternative splicing and expression
            analysis of the human gene WNK3 (PRKWNK3)
  JOURNAL   Gene 335, 109-119 (2004)
   PUBMED   15194194
  REMARK    GeneRIF: WNK3 lies within the critical linkage interval for several
            human monogenic disorders, including X-linked mental retardation.
            The function of mammalian WNK3 kinase remains to be investigated.
REFERENCE   7  (bases 1 to 11384)
  AUTHORS   Verissimo,F. and Jordan,P.
  TITLE     WNK kinases, a novel protein kinase subfamily in multi-cellular
            organisms
  JOURNAL   Oncogene 20 (39), 5562-5569 (2001)
   PUBMED   11571656
REFERENCE   8  (bases 1 to 11384)
  AUTHORS   Wilson,F.H., Disse-Nicodeme,S., Choate,K.A., Ishikawa,K.,
            Nelson-Williams,C., Desitter,I., Gunel,M., Milford,D.V.,
            Lipkin,G.W., Achard,J.M., Feely,M.P., Dussol,B., Berland,Y.,
            Unwin,R.J., Mayan,H., Simon,D.B., Farfel,Z., Jeunemaitre,X. and
            Lifton,R.P.
  TITLE     Human hypertension caused by mutations in WNK kinases
  JOURNAL   Science 293 (5532), 1107-1112 (2001)
   PUBMED   11498583
REFERENCE   9  (bases 1 to 11384)
  AUTHORS   Mulley,J.C., Kerr,B., Stevenson,R. and Lubs,H.
  TITLE     Nomenclature guidelines for X-linked mental retardation
  JOURNAL   Am J Med Genet 43 (1-2), 383-391 (1992)
   PUBMED   1605216
  REMARK    Review article
REFERENCE   10 (bases 1 to 11384)
  AUTHORS   Watty,A., Prieto,F., Beneyto,M., Neugebauer,M. and Gal,A.
  TITLE     Gene localization in a family with X-linked syndromal mental
            retardation (Prieto syndrome)
  JOURNAL   Am J Med Genet 38 (2-3), 234-239 (1991)
   PUBMED   1673297
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL591766.20, AY352048.1,
            Z84469.1 and R44257.1.
            
            On Aug 13, 2020 this sequence version replaced NM_020922.4.
            
            Summary: This gene encodes a protein belonging to the 'with no
            lysine' family of serine-threonine protein kinases. These family
            members lack the catalytic lysine in subdomain II, and instead have
            a conserved lysine in subdomain I. This family member functions as
            a positive regulator of the transcellular Ca2+ transport pathway,
            and it plays a role in the increase of cell survival in a
            caspase-3-dependent pathway. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY352048.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354646.7/ ENSP00000346667.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                AL591766.20        31446-31486         c
            42-6253             AY352048.1         1-6212
            6254-11298          Z84469.1           35053-40097         c
            11299-11384         R44257.1           14-99               c
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp11.22"
     gene            1..11384
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /note="WNK lysine deficient protein kinase 3"
                     /db_xref="GeneID:65267"
                     /db_xref="HGNC:HGNC:14543"
                     /db_xref="MIM:300358"
     exon            1..361
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            362..1017
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             481..5883
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein kinase WNK3; protein kinase with
                     no lysine 3; Prieto X-linked mental retardation syndrome"
                     /codon_start=1
                     /product="serine/threonine-protein kinase WNK3 isoform 1"
                     /protein_id="NP_065973.2"
                     /db_xref="CCDS:CCDS14357.1"
                     /db_xref="GeneID:65267"
                     /db_xref="HGNC:HGNC:14543"
                     /db_xref="MIM:300358"
                     /translation="MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNS
                     TFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQ
                     EVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKT
                     VYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILK
                     GKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDL
                     KCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAF
                     GMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSE
                     RLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEF
                     SFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRD
                     SQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGD
                     NLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHY
                     QQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVS
                     QPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEK
                     GTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLES
                     EKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQ
                     TSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEI
                     SRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAP
                     VTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGN
                     SLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQ
                     PTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFS
                     SSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDL
                     NVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKN
                     PISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEEMRSAIAPDPIPLTRESTADTR
                     ALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKS
                     QLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPE
                     KEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESN
                     QCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQ
                     TQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGI
                     VATGKSCLINELENPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIK
                     PSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPS
                     FPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAF
                     PTSSVQNPATIPPGPK"
     exon            1018..1190
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1411
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1569
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1658
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1878
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2173
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2174..2367
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2517
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..2582
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2583..2730
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..2847
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..3003
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3004..3055
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3193
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3194..4131
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            4132..4363
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            4364..4494
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            4495..5108
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            5109..5320
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            5321..5350
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            5351..5553
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     exon            5554..11384
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11325..11330
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /note="hexamer: AATAAA"
     polyA_site      11346
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /note="major polyA site"
     regulatory      11358..11363
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /note="hexamer: ATTAAA"
     polyA_site      11384
                     /gene="WNK3"
                     /gene_synonym="MRXS2; PRKWNK3; PRS"
                     /note="major polyA site"
ORIGIN      
        1 ctacccgtcc gaattccctg gctggagtac gcgtgcgtct gctcagctcc gggccgagcg
       61 cgagcttggg ctcgtacccc gctcccctct ctccgcccgc tctttaccta ccggccgtag
      121 ctggcccggt gggctcgcgg ggagacgctc gcgcgcgcaa gcgcacacga cgcggagcgg
      181 agctcgagac atcgaggaga ggagtaagaa gcatcggagg aggcgatcga ggaggaggcg
      241 gtgtgtgtgt atgtgtctat tctacggtgt ggctcgtcgt ggggcgagag gaggctcgag
      301 agagggcgag ggagaagcga aaagagtaag agagtcttcc tttgcgagag cctctaatct
      361 ggttgctatg gaaatacatg accacgcaaa aggaagtcca ttctgataat tctgatacct
      421 gagatgtaac tggactgaag agtagaaaca ggaaaaattt tagtgccaac tttaattaca
      481 atggccactg attcagggga tccagccagc acagaagatt ctgagaaacc tgatggaatt
      541 tcatttgaaa acagagttcc ccaggtcgct gcaactttga cagtagaagc tagactaaag
      601 gagaaaaaca gtaccttctc tgcttctggg gaaactgtag aaaggaagag atttttccga
      661 aagagtgttg aaatgacgga agatgacaaa gttgccgaat catcccccaa agatgagaga
      721 attaaggctg caatgaatat tccaagagta gataagcttc cttcaaatgt gttgagaggt
      781 ggacaagaag ttaaatatga acagtgttca aagtcaacct cagaaatctc aaaagattgt
      841 ttcaaggaga aaaatgaaaa ggaaatggaa gaagaagcag aaatgaaggc tgtagctact
      901 tctcctagtg gcagattcct gaaatttgac atagaactag gaagaggagc atttaaaaca
      961 gtatataaag gactggacac tgaaacatgg gttgaggttg cttggtgtga gctgcaggac
     1021 cgaaagttaa ccaaagctga gcagcaaaga ttcaaggaag aagcagagat gttgaagggt
     1081 ctccagcacc ccaatatagt tcgattttat gattcctggg aatctatatt aaaaggaaag
     1141 aaatgtattg tattagtgac tgaactaatg acatctggga ccttaaagac gtacttaaaa
     1201 cgatttaaag tcatgaaacc aaaggtctta aggagctggt gcaggcaaat tttaaagggg
     1261 ttgcagttct tgcacactag gactcctcct attattcacc gggatctgaa gtgtgacaat
     1321 attttcatca cgggacccac tggatctgtg aagattggtg atctaggatt agccacctta
     1381 atgcgtacct catttgctaa gagtgtcatt ggaactcctg agtttatggc tccagagatg
     1441 tatgaagaac actatgatga atccgtagat gtttatgctt ttggaatgtg tatgctggaa
     1501 atggccacat cggagtatcc ttattctgag tgtcagaatg cagctcaaat ataccggaaa
     1561 gtaactagtg gcataaaacc agccagcttc aataaagtca ctgatcctga agtcaaagaa
     1621 atcattgaag gatgtattcg tcaaaacaaa tctgaaaggt tgtctatcag ggacctatta
     1681 aaccatgcat tttttgctga ggatacagga ctgagggtgg agttagcaga agaagatgat
     1741 tgctcaaatt catcccttgc tttaagactc tgggttgaag accctaaaaa attgaaaggc
     1801 aaacacaaag acaatgaagc tattgaattt agtttcaact tagaaacaga tacacctgag
     1861 gaagtagcat atgaaatggt caagtctggg ttcttccatg aaagtgattc caaagctgtt
     1921 gctaaatcca ttagagaccg ggtgacgcca ataaagaaga caagagagaa gaagcctgct
     1981 ggctgtttgg aagaacgcag ggattctcag tgcaagtcta tggggaatgt attccctcag
     2041 ccccagaata caactttacc ccttgctccc gctcagcaaa ctggggctga atgtgaagaa
     2101 actgaagttg atcaacatgt tagacaacag cttctacaaa gaaaaccaca gcagcactgc
     2161 tcctctgtta caggtgacaa tttgtctgag gcaggagctg catcagttat acattcagat
     2221 acttcaagtc agcccagtgt agcctattcc tcaaatcaaa cgatgggctc tcaaatggtt
     2281 tctaatatcc cgcaggctga agtaaatgtt ccagggcaaa tttattcatc tcagcaacta
     2341 gtaggacatt accagcaagt ttcagggtta cagaagcatt caaagctgac tcagccgcag
     2401 attttgcctt tggttcaagg tcagtccact gttttacctg tacatgtcct tggaccgaca
     2461 gttgtttcac aaccccaggt ttccccatta actgttcaga aggtcccaca gataaagcct
     2521 gtatcccaac cagttggagc tgaacaacaa gcagctcttc taaaaccaga tttagttcga
     2581 agcttgaatc aagatgtggc aactacgaag gaaaacgtca gtagccctga taacccaagt
     2641 ggaaatggca aacaggatcg gatcaaacag agaagagctt cctgtccccg accagagaag
     2701 gggactaaat ttcagcttac cgtccttcag gtatcaacct ctggagataa catggtagag
     2761 tgtcagctgg agacacacaa caacaagatg gtcaccttca agtttgatgt tgatggtgat
     2821 gcgccagagg atattgcaga ctatatggtt gaagataact ttgtgctgga aagtgagaaa
     2881 gaaaaatttg tagaagaatt gagagctatt gtaggtcaag cccaggagat ccttcatgtc
     2941 cactttgcca cagaaagagc cactggagtt gattctatta ctgtggactc caacagtagc
     3001 cagacagggt catctgaaca agtacagata aattctacat ctactcaaac cagcaatgaa
     3061 tctgctcctc agtcatcccc agttggtcgg tggcgattct gtatcaatca gacgataaga
     3121 aaccgtgaga ctcagtctcc tccttctctt cagcattcca tgtctgcggt tcctggccga
     3181 catccacttc ctagtccaaa aaacacaagt aataaggaaa tatcacggga cacattgctc
     3241 actatagaaa ataatccatg ccaccgtgca cttttcacct ccaaatcaga acacaaggat
     3301 gtggttgatg gtaagatttc tgaatgtgct agtgtagaaa ccaagcagcc agctatactt
     3361 tatcaagtgg aagataacag gcagataatg gcaccagtta ctaatagttc cagttactct
     3421 actacttcag ttcgtgcagt tccagctgaa tgtgagggac tcaccaaaca agcaagcata
     3481 ttcatacctg tgtatccatg tcaccaaact gccagtcagg ctgatgcact tatgtcccat
     3541 cctggcgaat caactcagac ttctggtaac tctcttacaa ctctggcatt tgatcaaaag
     3601 cctcaaacct tatcagtaca gcagccagct atggatgcag agtttatttc tcaagaagga
     3661 gaaactacag tgaacactga agcaagttct cctaagacag tcattcccac tcagacccct
     3721 ggccttgaac caactaccct tcaacccact actgtcctgg aatcagatgg agaaagacct
     3781 ccaaaactgg agtttgcaga caaccgaatt aaaactctgg atgaaaaatt aagaaacttg
     3841 ctctatcagg agcacagcat ctctagcatc tatcccgaga gtcagaagga tacccaaagc
     3901 atagactctc cattttcttc ctctgctgaa gataccctct cctgtccagt gacagaagtc
     3961 atagccatca gtcactgtgg aattaaagat agccctgtac aatcccctaa tttccaacag
     4021 acaggctcta agcttctgtc caatgtggct gcaagtcagc ctgctaatat atcagtgttc
     4081 aaaagggacc tgaatgtgat aacttctgta cccagcgaat tgtgtttaca tgagatgtcc
     4141 tcagatgctt cacttccagg ggatccagag gcctatcctg ctgctgtgtc aagcggtgga
     4201 gccattcatc tgcagacagg aggtggatat tttggcctaa gctttacttg tcctagtctc
     4261 aaaaatccta ttagcaagaa atcctggact cgcaaattaa aaagctgggc atacaggcta
     4321 cggcagtcaa ccagcttttt caagagatca aaagtccgtc aagtggaaac agaagagatg
     4381 agatcagcaa ttgctcctga tcccattcct ctgacacggg agtccacagc tgatactagg
     4441 gctttgaata gatgtaaagc gatgagtgga tcatttcagc ggggtcggtt ccaggtgatt
     4501 acaattcctc agcagcagtc agcaaaaatg acatcttttg gaatagaaca catatcagtg
     4561 ttcagtgaga caaaccattc tagtgaagaa gcctttatta aaacagcaaa gtctcagttg
     4621 gtagaaatag aacctgccac acaaaatcca aaaacttcgt tttcttatga gaagttacaa
     4681 gctcttcagg aaacctgtaa agaaaataaa ggagttccca aacaaggtga caacttctta
     4741 tctttcagcg cagcttgtga gactgatgta tcttcagtga ccccagaaaa ggaatttgaa
     4801 gaaacttcag ccacaggaag tagcatgcag tctggatctg aactgttgct taaagagaga
     4861 gagatattga ctgctgggaa acagcctagc tctgatagtg aattttcagc cagtcttgct
     4921 ggcagtggaa agtcagtggc aaagactggt ccagagagta atcagtgctt accacaccac
     4981 gaagaacaag cttatgctca aacacagagt tcactcttct attcgccatc ttccccaatg
     5041 agcagtgatg atgaatcaga aatagaggat gaggacttga aggtggagct tcaaagatta
     5101 cgagaaaaac acattcagga ggtggtaaat cttcaaaccc agcagaataa ggagctgcag
     5161 gagctctatg aacgccttcg gtcaattaaa gatagcaaaa cccaatctac tgagattcct
     5221 ttgccacctg catcaccacg tcgaccaaga tctttcaaaa gcaaacttcg aagccgcccc
     5281 cagtccttga cacatgtgga caatggcata gttgctacag ggaaaagctg ccttataaat
     5341 gagttggaaa atccactgtg tgtggagagt aatgcagcat catgccaaca gtctccagcc
     5401 agtaaaaaag ggatgttcac agatgactta cacaagctgg tggatgactg gacaaaggaa
     5461 gcagtaggaa attctcttat taagccaagt ttaaaccaac ttaaacaaag tcaacacaaa
     5521 ctagagacag aaaactggaa taaagtatct gaaaatactc cgtctactat gggctacaca
     5581 tcaacatgga tttcttctct gtcccaaatc cgtggagctg tcccaacttc cttgccacaa
     5641 ggactctcac tcccttcatt tcctgggcca ttatcatcat atggaatgcc tcacgtttgt
     5701 cagtataatg ctgtggcggg ggcggggtat ccagtacagt gggtaggaat ttcaggaaca
     5761 acacaacaat ctgtagtaat tcccgcccaa tctgggggac cattccagcc agggatgaat
     5821 atgcaggcat ttccaacttc atcagtgcag aatcctgcca caatccctcc tggtcctaaa
     5881 tgaatcagag tagatggtga taaaaaggga ggttttttca gaattatttt caggacaccc
     5941 aagatattaa agtttcttcc tcttgggttc tgtcatattt tccctcattt gagtttactt
     6001 tagactatat attttttgtt ccagtgtggt atttgataca gctcatcatt ctgtagaaat
     6061 gtgcagtttg cagatagaac actgcacaca gaaatgtttt ttcacttcaa attctatcct
     6121 ctttgatact tgatttttta aaaatactgt tcagaatgct ttaataagct cagcttgagc
     6181 attaccattt ccaggacact ttccatgaat tgttgagaag ctccccatcc cccgcctttt
     6241 tttttttttt ttttaactgc tgctatctgc agctggatac tcttctttaa aaatgtttaa
     6301 aaattattta gattgattct ccccaaatgt ggatgaactg agaccctcca tcagccagag
     6361 tcttatggga tcttcacttc acatgtttgt cttaggttta cctttcacag aaggcagggt
     6421 aacttgctgc aggaaacgtt ttggtgtata aaattagaat cacttttttt tttttttttg
     6481 agatggagtc tctctctgtc acccaggttg gagtgcagtg gtgtgatctt ggctcactgc
     6541 tgcaacctcc ccctcctggg ttcaagcgat tctcctgcct cagcctcctg agtagctggg
     6601 actacaggcg ccagccacca tgcctggcta atttttgtat ttttagtaga ggatggggtt
     6661 tcactatgtt ggccagactg gtctcaaact cctaaccttg tgatccgccc tccttagccc
     6721 ccccaaaaaa ttacaataat tttgtcaacc aatacagaac atgagccctt tttaaatgtg
     6781 atttcccgtc atcacagcag taggatgaag ccttgttttg ggtgagacag agggaaaggg
     6841 aaaaaacaga attgtaacag ttccttgatt ctgcagatac tgctgctgtt ttacgatttc
     6901 agtaataatt caaagcagct gtctccttgt ttctcacatg catactacat tggttttgag
     6961 catctgagag gcattggttt gtcttgcatt cttttgcaag gcatatattg cagctgatat
     7021 gaatctcgtt tggggggaat ccttgtattc ctaacaaggg ggattaaagc tacacctcaa
     7081 tccattcttc tgaagtgaaa tttacttgac acagtaggtt tcgagactta catatgaaac
     7141 actggcttta cagggttctc agagatgtgg ggtatacact gtcctgcagt gagatagtag
     7201 accctagaag gatactattt aagttgtcct tagctctttt taattacaat ttttagttgt
     7261 tagaatttct ttttagtatc tatagtatct tagatgattt tagatcttaa atgagtttgg
     7321 cagcctcttt agaatctgag agagatcact gtcaagttgc actttactgg gttttatcca
     7381 gttttaggag gaggggaggc aatgtcaaaa tgtacacctt tggtttttaa gaaggttaac
     7441 tataataatg ttaaaggcta gtcacagggt tacctaatgc tcagtctgtg aaacctaaat
     7501 agaattctta aaatgttatt taccaactaa gtattcatgt tcaaattact taccttccac
     7561 tctgaagact ttaaggtcat ttctaataca catttaccta attaggagtt ctgtctacta
     7621 gtaaacattc caaattgcta cccaacatta ctgttaacag attttcctcc gtattcttct
     7681 aaaaattata ccccatgggc ttgccaggta atgacgaaaa cttaatgggt gtactttttt
     7741 ttttttttgc acagtctact ttgtgacatt aagtacctct tttccaaaaa aaaaaaaaaa
     7801 tgtgttttat gttgcttacc tgaggatcac atccattatc aagaagattc tgtcgctata
     7861 atttgaggca tctagggcag cattttgaga attgctgttg ataagatcca ctgtttcttt
     7921 tcatagaaca tgatctgttt tgtatgagtc tttccaaatg ttaagtacag attcttcaaa
     7981 tttcttgtac agtattggtt tgcagtttaa agtgatttta ggacattagt gtaatgcaaa
     8041 tgcttgcatt gcactgtttt ccatatgtat aaaaacaatg ctcctaaaat ccctaattct
     8101 ttaaaactgc ctgtgtttca ttataaaaag ttggctttgt gtaattacac cgagccatac
     8161 aacctataaa gtgaaaatac tgcagatttt aggacccctt cttctctagt cacaatcatt
     8221 ttaaacagtg taccccagga tgaaaaaagc tccatggctg acatgtacaa ttacatgtat
     8281 taatgactgc tctacatcct atgttctgcc tttggccacc acctctattc cacatataat
     8341 tcatcattct tgtttgcctg cagactcttg cagtgccttg taatatttac aatcttttca
     8401 ggtttttgct ggaaagaaaa gtagtcttct aaagtttgaa gatggtgaat agtgtactgc
     8461 tgtctccatg ctgactgcag taaccgggtt atcatatatg ttatgtatac attatgtata
     8521 tataacatgt gtacatttaa aaatacagaa aaaagtgagc ttgtgcagaa atggagaaaa
     8581 caatgttatt ctcatcactg tggggtttta ttaaaattat tttttcccat gacttacatg
     8641 tttggttcag attatagtca ctttgactgt aaatactggc tagttttcta tccagggctt
     8701 ttgcattatt tcataatttt gctaaatcat ataatagtgc tattttcatc cttgtctgag
     8761 gtacttttcc tccccagaaa taattaactt gaggtaaagc tcagaaattc ccttcttaac
     8821 aaagagccta ctatataaaa agggccttcc tttccaaaaa acacacaaac ctgtcctttt
     8881 cattttcctt cttgtagagg agaaaatggt tgtacttaac catcctgctt gtaaagttta
     8941 ttcagaggat agtctgtatg tccagccacc atgtttactt ctcaaagtgc aagtctgatg
     9001 aatagtactg tatctccata tgtggtaatg tgagcatttc atcatgaagt acctgctgct
     9061 atgtctgtga agttgggtgt aacatcataa gaatatgaaa ccagtattgg tgtaaatcta
     9121 atgaaaataa ccctatgtat atttgtagca gtcttgaatc tctatttaag gtaatttgct
     9181 gtaatataac aaatattcat tctgtttttc atcccttcct tgccagtttt atatcaagca
     9241 tgaattccaa tggtttgaca gcaaaattat ctgtgaaagt ttaaaaggca cttttctgct
     9301 gtgatttttg gattgagtgt ggagtgttat gtacagtttg atgggatata tcattttaga
     9361 accccagatt taattctagt ccaattaatt ttgtttaact acctcgtttt cagtgctgtt
     9421 ttgggtgcac aagtaaacac tacctgtgaa acttgctttt ttgccttgat tctcagtatt
     9481 ctttcagatt ataaatctgg tctgcctctt ttttcccagt gctagccaac agtttgagat
     9541 gagagggatc atcactctca tctgtgttta agtggagagc catgtgcttc tgtgaaatat
     9601 gctttgggga tttgggggtg gagagcagaa gaataattga aattggtggc aatataggat
     9661 ttaggtgtag gagttagtta taaaataaac aaatggtaag aaagtacctg aattagacaa
     9721 tccagtgcct ataactttgc attttttatt agagatgcat ttctcgtctg tgtgcctaaa
     9781 gggcaatggg ttgaaattgc tttaactacc acagcgaaat atttgacaaa tccttcagca
     9841 atggcctatc attcaacata tattagcaca gtgctaggta tttgcagtgg tattttgtta
     9901 ccttaaaacg gccaaatgca tttagttgaa atgtagttga tagtaagggt tttatataac
     9961 tttatccaat ctctaaagtt tatggaagca ttgactttct tctttgctgt cttttctgag
    10021 tgatgaaaat gatgcattcc ttttttttcc ttgaaaactc agcaaatgtt tgtaaccaga
    10081 tgttccttgt ttcacatgtt tactgtgtaa cactacagca tatttaatat tggtaaatac
    10141 tgtatttcag tcattatgca taagtacatt cctgaggtat atttgccatc attttgggat
    10201 tcagttattt tatcttctgt ttgggtcaga aaaacttgaa ctatctgagg tggtatgcag
    10261 gccattttct agaagattgt tttttgaatc tgtagtttat caaggacttg ttccccccct
    10321 ccctttgttt agaagaggaa tcagaaaagg ggggtggtgt gtagaaaagg cctttgattt
    10381 gataggtgta atttctaaga ttgaaatttt aaaaatgcag atcgtaagat agtaacactc
    10441 aaacttgata aaatcctttg aattgaaatt agtatgaaat ttttatggaa ccaaatgcct
    10501 tcaggtcacc ctggaaatag tctttgtatg tgaaagatca acacaaacat tggtcaggaa
    10561 ccctgacagc caatatatga aggagcctgg gatagtaact aggttacact aaagttattt
    10621 tcaactaaga tagctagacg agtgtacaaa ccaatagctc ctattctgga aggttttctt
    10681 tttattaaaa aaaattcaaa caaggttaaa agtcaagcaa gaagggaaga gagaaactgg
    10741 gttctgagaa aaaaatgtgc cagtataaaa taaactccta aatgcgtgct tgtcatcctc
    10801 tagttttttt ttaagttgaa tttcttttcc actgtaactt aagatttgag attgaggttt
    10861 gcggtccaga acataccctc agcagataca gtgactaact ggaaagtgca gttgttcaag
    10921 gtctgtcatg ctcaatcacc taaagctata atttgtttga tatattaagc atgtagacct
    10981 agtgcagcat gggagccact caggaagttt atgcaattaa taaactttca gcataattta
    11041 ctatgaagta tgcagaattt caccctcttc tccacactta acatttagtt gtatatgtga
    11101 actctccttt cttaattggg gaatgtagca ttatatagaa tgttgttaaa gttaatttta
    11161 atcctttttg acattaacct tttttttttt ttggtaaacc aagtgatctg cctttcagca
    11221 actgtcttat tttggttctt tgaaactgtg atttttattt catttgtacc caaccattgt
    11281 aagtattttg ttttgtttcc tggaaattct gttttgaaac agaaaataaa ggctgtgtta
    11341 aatgagtatg ttatcttatt aaaatgctgc catagtcact gcaa
//
