LOCUS       NM_006648               7080 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens WNK lysine deficient protein kinase 2 (WNK2),
            transcript variant 2, mRNA.
ACCESSION   NM_006648 XM_005252141
VERSION     NM_006648.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Zhu,L., Chen,C., Wu,S., Guo,H., Li,L., Wang,L., Liu,D., Zhan,Y.,
            Du,X., Liu,J., Tan,J., Huang,Y., Mo,K., Lan,X., Ouyang,H., Yuan,J.,
            Chen,X. and Ji,J.
  TITLE     PAX6-WNK2 Axis Governs Corneal Epithelial Homeostasis
  JOURNAL   Invest Ophthalmol Vis Sci 65 (12), 40 (2024)
   PUBMED   39453672
  REMARK    GeneRIF: PAX6-WNK2 Axis Governs Corneal Epithelial Homeostasis.
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Mo,H.Y., Moon,S.W., An,C.H. and Lee,S.H.
  TITLE     Regional bias of tumor suppressor gene mutations of STARD8 and WNK2
            in colon cancers
  JOURNAL   Pathol Res Pract 253, 155000 (2024)
   PUBMED   38091885
  REMARK    GeneRIF: Regional bias of tumor suppressor gene mutations of STARD8
            and WNK2 in colon cancers.
REFERENCE   3  (bases 1 to 7080)
  AUTHORS   Soares de Lima,Y., Arnau-Collell,C., Munoz,J., Herrera-Pariente,C.,
            Moreira,L., Ocana,T., Diaz-Gay,M., Franch-Exposito,S.,
            Cuatrecasas,M., Carballal,S., Lopez-Novo,A., Moreno,L.,
            Fernandez,G., Diaz de Bustamante,A., Peters,S., Sommer,A.K.,
            Spier,I., Te Paske,I.B.A.W., van Herwaarden,Y.J., Castells,A.,
            Bujanda,L., Capella,G., Steinke-Lange,V., Mahmood,K., Joo,J.E.,
            Arnold,J., Parry,S., Macrae,F.A., Winship,I.M., Rosty,C.,
            Cubiella,J., Rodriguez-Alcalde,D., Holinski-Feder,E., de Voer,R.,
            Buchanan,D.D., Aretz,S., Ruiz-Ponte,C., Valle,L., Balaguer,F.,
            Bonjoch,L. and Castellvi-Bel,S.
  TITLE     Germline mutations in WNK2 could be associated with serrated
            polyposis syndrome
  JOURNAL   J Med Genet 60 (6), 557-567 (2023)
   PUBMED   36270769
  REMARK    GeneRIF: Germline mutations in WNK2 could be associated with
            serrated polyposis syndrome.
REFERENCE   4  (bases 1 to 7080)
  AUTHORS   Rehman,T., Karp,P.H., Thurman,A.L., Mather,S.E., Jain,A.,
            Cooney,A.L., Sinn,P.L., Pezzulo,A.A., Duffey,M.E. and Welsh,M.J.
  TITLE     WNK Inhibition Increases Surface Liquid pH and Host Defense in
            Cystic Fibrosis Airway Epithelia
  JOURNAL   Am J Respir Cell Mol Biol 67 (4), 491-502 (2022)
   PUBMED   35849656
  REMARK    GeneRIF: WNK Inhibition Increases Surface Liquid pH and Host
            Defense in Cystic Fibrosis Airway Epithelia.
REFERENCE   5  (bases 1 to 7080)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 7080)
  AUTHORS   Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F.,
            Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M.
            and Zoghbi,H.Y.
  TITLE     A protein-protein interaction network for human inherited ataxias
            and disorders of Purkinje cell degeneration
  JOURNAL   Cell 125 (4), 801-814 (2006)
   PUBMED   16713569
REFERENCE   7  (bases 1 to 7080)
  AUTHORS   Verissimo,F. and Jordan,P.
  TITLE     WNK kinases, a novel protein kinase subfamily in multi-cellular
            organisms
  JOURNAL   Oncogene 20 (39), 5562-5569 (2001)
   PUBMED   11571656
REFERENCE   8  (bases 1 to 7080)
  AUTHORS   Wilson,F.H., Disse-Nicodeme,S., Choate,K.A., Ishikawa,K.,
            Nelson-Williams,C., Desitter,I., Gunel,M., Milford,D.V.,
            Lipkin,G.W., Achard,J.M., Feely,M.P., Dussol,B., Berland,Y.,
            Unwin,R.J., Mayan,H., Simon,D.B., Farfel,Z., Jeunemaitre,X. and
            Lifton,R.P.
  TITLE     Human hypertension caused by mutations in WNK kinases
  JOURNAL   Science 293 (5532), 1107-1112 (2001)
   PUBMED   11498583
REFERENCE   9  (bases 1 to 7080)
  AUTHORS   Ito,M., Shichijo,S., Tsuda,N., Ochi,M., Harashima,N., Saito,N. and
            Itoh,K.
  TITLE     Molecular basis of T cell-mediated recognition of pancreatic cancer
            cells
  JOURNAL   Cancer Res 61 (5), 2038-2046 (2001)
   PUBMED   11280764
REFERENCE   10 (bases 1 to 7080)
  AUTHORS   Scanlan,M.J., Chen,Y.T., Williamson,B., Gure,A.O., Stockert,E.,
            Gordan,J.D., Tureci,O., Sahin,U., Pfreundschuh,M. and Old,L.J.
  TITLE     Characterization of human colon cancer antigens recognized by
            autologous antibodies
  JOURNAL   Int J Cancer 76 (5), 652-658 (1998)
   PUBMED   9610721
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL390760.25, AL354991.11 and AL583839.12.
            
            On or before Oct 23, 2020 this sequence version replaced
            XM_005252141.2, NM_006648.3.
            
            Summary: The protein encoded by this gene is a cytoplasmic
            serine-threonine kinase that belongs to the protein kinase
            superfamily. The protein plays an important role in the regulation
            of electrolyte homeostasis, cell signaling survival, and
            proliferation. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Sep 2013].
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            coding region and uses an alternate splice site in the 3' coding
            region, compared to variant 1. This results in a shorter protein
            (isoform 2), compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1968540,
                              SAMEA1968832 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000427277.7/ ENSP00000411181.4
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-247               AL390760.25        85136-85382
            248-930             AL390760.25        85925-86607
            931-1103            AL390760.25        130693-130865
            1104-1324           AL390760.25        131885-132105
            1325-1482           AL390760.25        135805-135962
            1483-1571           AL390760.25        139230-139318
            1572-1791           AL390760.25        140754-140973
            1792-2083           AL390760.25        148540-148831
            2084-2283           AL390760.25        153880-154079
            2284-2439           AL390760.25        157296-157451
            2440-2631           AL390760.25        157945-158136
            2632-3315           AL390760.25        159928-160611
            3316-3609           AL390760.25        162811-163104
            3610-3659           AL390760.25        163667-163716
            3660-3828           AL390760.25        164563-164731
            3829-3945           AL390760.25        164914-165030
            3946-4116           AL390760.25        168743-168913
            4117-4162           AL390760.25        169017-169062
            4163-4282           AL390760.25        169624-169743
            4283-5115           AL354991.11        15988-16820
            5116-5185           AL354991.11        17178-17247
            5186-5274           AL354991.11        19508-19596
            5275-5957           AL354991.11        19691-20373
            5958-6172           AL354991.11        25053-25267
            6173-6364           AL354991.11        26270-26461
            6365-6463           AL354991.11        27251-27349
            6464-6508           AL354991.11        33977-34021
            6509-6765           AL354991.11        35528-35784
            6766-6877           AL583839.12        9776-9887
            6878-7080           AL583839.12        12623-12825
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q22.31"
     gene            1..7080
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /note="WNK lysine deficient protein kinase 2"
                     /db_xref="GeneID:65268"
                     /db_xref="HGNC:HGNC:14542"
                     /db_xref="MIM:606249"
     exon            1..247
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            248..930
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     CDS             250..6903
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     serologically defined colon cancer antigen 43;
                     mitogen-activated protein kinase kinase kinase;
                     serine/threonine-protein kinase WNK2; antigen NY-CO-43;
                     protein kinase with no lysine 2; protein kinase
                     lysine-deficient 2"
                     /codon_start=1
                     /product="serine/threonine-protein kinase WNK2 isoform 2"
                     /protein_id="NP_006639.3"
                     /db_xref="GeneID:65268"
                     /db_xref="HGNC:HGNC:14542"
                     /db_xref="MIM:606249"
                     /translation="MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRR
                     SVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALV
                     AQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAE
                     AKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETW
                     VEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTE
                     LMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGP
                     TGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATS
                     EYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSH
                     AFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPD
                     EVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARG
                     PPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTV
                     YSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLP
                     VGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPL
                     PQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPI
                     PVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPL
                     QAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPA
                     AFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQP
                     TRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAP
                     YAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTA
                     TLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSG
                     KELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVEC
                     QLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLS
                     EDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAE
                     HPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPG
                     APPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPL
                     AETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALG
                     QPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSL
                     DEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKE
                     AVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSH
                     VVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARAL
                     GSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDV
                     KLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATA
                     FLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSL
                     REKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGK
                     LLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSV
                     RASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSA
                     LKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEW
                     TSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPG
                     PLSTTVIPGAAPTLSVPTPDPESEKPD"
     exon            931..1103
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1324
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1482
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1571
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1791
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..2083
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2283
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2439
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..2631
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            2632..3315
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            3316..3609
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            3610..3659
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            3660..3828
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            3829..3945
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            3946..4116
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            4117..4162
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            4163..4282
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            4283..5115
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            5116..5185
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            5186..5274
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            5275..5957
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            5958..6172
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            6173..6364
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            6365..6463
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            6464..6508
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            6509..6765
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            6766..6877
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     exon            6878..7080
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7056..7061
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /note="hexamer: AATAAA"
     polyA_site      7080
                     /gene="WNK2"
                     /gene_synonym="NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43"
                     /note="major polyA site"
ORIGIN      
        1 ctccccgcgc gccgggtcgg agccggtgcg ggagcggagc cgcgcgaagc cggcaggagc
       61 acgcgggagc gcggccccgc agtggcccgg cccgagagag cagcgcgcag gagctggagc
      121 ggcgccacgg cccccacccc agcgcggacc tcgcccggaa ctcggacccc gtcctcgaag
      181 cgtgatctct cccgcctcgc acgccctggc cgccgggccg cgggcatgga cggcgtccgc
      241 tcgccctaga tggacggcga tggcggccgc cgagacgtcc ccggcacgct gatggagccc
      301 gggcgcggcg cggggcccgc gggcatggcg gagcctcggg cgaaggcggc gcggccgggg
      361 ccccagcgct ttctgcggcg cagcgtggta gagtcggacc aggaggagcc gccgggcttg
      421 gaggcagccg aggcgccggg cccgcagccc ccgcagcccc tgcagcgccg ggtgcttctg
      481 ctctgcaaga cgcgccgcct catcgcggag cgcgcccgcg gacgccccgc cgcccccgcg
      541 cccgcagcgc tggtagcgca gccgggagcc cccggagccc ccgcggacgc cggccccgag
      601 cccgtgggca cgcaggagcc cggcccggac cccatcgcag ccgctgtcga aaccgcgcct
      661 gcccccgacg gcggccccag ggaggaggcg gcggcgaccg tgaggaagga ggatgagggg
      721 gcggccgagg cgaagcctga gcccgggcgc actcgccggg acgagcccga agaggaggag
      781 gacgacgagg acgacctcaa ggccgtggcc acctctctgg acggccgctt cctcaagttc
      841 gacatcgagc tgggccgcgg ttccttcaag acggtctaca aggggctgga cacggagacc
      901 tgggtggagg tggcctggtg tgagctgcag gaccggaagc tcaccaagct ggagcggcag
      961 cggttcaagg aagaggctga gatgctgaaa ggcctgcagc accccaacat cgtgcgcttc
     1021 tacgacttct gggagtccag cgccaagggc aagcggtgca ttgtgctggt gacggagctg
     1081 atgacctcag ggacgctgaa gacatacctg aagcggttca aggtgatgaa gcccaaggtt
     1141 ctccgcagct ggtgccggca gatcctgaag ggcctgctgt tcctgcacac aaggacgcca
     1201 cccatcatcc accgagacct gaaatgtgac aatattttca tcaccggacc aactgggtct
     1261 gtgaagattg gcgacttggg cctggccact ctgaaaagag cgtcatttgc caaaagtgtg
     1321 ataggtactc ccgagttcat ggcgcccgag atgtacgagg agcactacga tgagtccgtg
     1381 gacgtctatg cctttgggat gtgcatgctg gagatggcca cctcggagta cccctactcg
     1441 gagtgccaga atgcggccca gatctaccgc aaggtcacct gtggtatcaa gccggccagc
     1501 tttgagaaag tgcacgatcc tgaaatcaag gagattattg gggagtgtat ctgcaaaaac
     1561 aaggaggaaa ggtacgagat caaagacctg ctgagccacg ccttcttcgc agaggacaca
     1621 ggcgtgaggg tggagctcgc ggaggaggac cacggcagga agtccaccat cgccctgagg
     1681 ctctgggtgg aagaccccaa gaaactgaag ggaaagccca aggacaatgg agccatagag
     1741 ttcaccttcg acctggagaa ggagacgccg gatgaggtgg cccaagagat gattgagtct
     1801 ggattcttcc acgagagtga cgtcaagatc gtggccaagt ccatccgtga ccgcgtggcc
     1861 ttgatccagt ggcggcggga gaggatctgg cccgcgctgc agcccaagga gcagcaggat
     1921 gtgggcagcc cggacaaggc caggggtccg ccggtgcccc tgcaggtcca ggtgacctac
     1981 catgcacagg ctgggcagcc cgggccacca gagcccgagg agccggaggc cgaccagcac
     2041 ctcctgccac ctacgttgcc gaccagcgcc acctccctgg cctcggacag caccttcgac
     2101 agcggccagg gctctaccgt gtactcagac tcgcagagca gccagcagag cgtgatgctt
     2161 ggctcccttg ccgacgcagc gccgtccccg gcccagtgtg tgtgcagccc ccctgtgagc
     2221 gaggggcccg tcctgccgca gagcctgccc tcgctggggg cctaccagca gcccacggct
     2281 gcacctggct tgccggtggg ctctgtcccg gcccccgcct gccctccgtc cctccagcag
     2341 cacttcccgg atccggccat gagcttcgcc cccgtgctgc cgccgcccag cacccccatg
     2401 cccacgggcc caggccagcc agcacccccc ggccagcagc ctcctccgct ggcccagccg
     2461 acacccctgc cgcaggtcct ggccccacag cccgtggtcc ccctccagcc ggttcccccc
     2521 cacctgccac cgtacctggc tccagcctcc caggtggggg cccccgctca gctgaagccc
     2581 ctccagatgc cacaggcgcc cctgcagccg cttgctcaag tccctccgca gatgcccccg
     2641 attcctgttg tgccccccat cacgcccctg gcgggaatcg acggcctccc tccggccctc
     2701 ccagacctgc cgaccgcgac tgtgcctccc gtgccaccac ctcagtattt ctctccagcc
     2761 gtgatcttgc cgagcctcgc tgccccactc ccccctgcgt ccccagcctt gcctctgcag
     2821 gctgtgaagc tgccccaccc ccctggggcg cccctggcca tgccctgccg gaccattgtg
     2881 ccaaatgcac cggccactat ccccctgctg gccgtagccc caccgggcgt ggctgccctg
     2941 tccattcatt ctgccgtggc ccagctccca ggccaacctg tgtacccagc ggccttccca
     3001 cagatggcgc ctactgacgt ccctccttcc ccccatcaca cggtgcagaa tatgagggcc
     3061 acccctccac agccggcact gcctccacaa cccacactgc ccccacaacc cgtgctgccc
     3121 ccgcaaccca cgctgccccc tcaacctgtg ttgcccccgc aacccacacg gccccctcaa
     3181 cctgtgctgc ccccgcaacc catgctgccc ccacaacctg tgctgccccc gcagccggca
     3241 ctgcctgtgc gccctgagcc cctccagccc caccttcctg aacaagctgc tccagctgct
     3301 acaccaggga gccagattct gcttggccac ccagctccct atgctgtgga cgtcgccgct
     3361 caggtcccca ccgtgcctgt gccaccggct gcggtcctct cgccgcctct gccggaagtg
     3421 ctgctgcctg ccgcccctga gctcctgcct cagttcccca gctccctggc cacggtgtct
     3481 gcctctgtgc agagtgtgcc cacccagact gccacacttc tgccaccagc aaacccaccg
     3541 ctgcctggcg ggcccgggat cgccagccct tgcccaactg tccagctgac ggtggaacca
     3601 gtccaagagg agcaggcctc acaggacaag ccgcccggcc tcccgcagag ctgtgagagc
     3661 tatggaggtt ctgatgtcac ttctggaaaa gagctgagtg acagctgtga aggcgccttt
     3721 ggagggggca ggctggaggg cagggcagcc cgaaaacacc accgcaggtc cacgcgtgcg
     3781 cgctcccggc aggagagggc cagccggccc cggcttacca tcttgaacgt gtgcaacact
     3841 ggggacaaga tggtggagtg ccagctggag acgcacaacc acaagatggt gaccttcaag
     3901 ttcgacttgg acggggacgc acccgatgaa attgccacgt atatggtgga gcatgacttt
     3961 atcctgcagg ccgagcggga aacgttcatc gagcagatga aggatgtcat ggacaaggca
     4021 gaggacatgc tcagcgagga cacagacgcc gaccgtggct ccgacccagg gaccagcccg
     4081 ccacacctca gcacctgcgg cctgggcacc ggggaggaga gccgacaatc ccaagccaac
     4141 gcccccgtgt atcagcagaa cgtcctgcac accgggaaga ggtggttcat catctgtccg
     4201 gtggctgagc accccgcccc cgaggcccct gaatcttcgc ccccacttcc tctaagctcc
     4261 ctgccgccag aagccagcca agattcagcg ccctataaag accagctgtc ctcgaaggaa
     4321 caacccagct ttctagccag tcagcagctc ctgagccagg cgggccccag caaccctcct
     4381 ggggcacccc cagccccttt ggccccctcc tcccctcctg tgactgctct gccccaagat
     4441 ggagcagctc cagccaccag caccatgcca gagccagcgt caggaactgc cagccaggca
     4501 gggggtccag ggacacctca ggggctgacc agtgagctcg agacgtctca gccactagcg
     4561 gagactcacg aggccccgct tgctgtgcag cccctcgtgg tgggcctagc accttgcact
     4621 ccagctccag aggctgcctc aaccagggac gccagtgccc caagggagcc cctgccacct
     4681 cctgcacctg agcccagccc ccacagcggg accccacagc ccgccttggg tcaacctgct
     4741 cccctgcttc ctgccgcagt gggggccgtc agcctggcca cctcccagct cccaagccca
     4801 cccctggggc ccaccgtccc cccacagcca ccctcggccc tggagtcgga tggggaaggg
     4861 ccgcccccca gggtgggctt tgtggacagc accatcaaga gcctggacga gaagctgcgg
     4921 actctgctct accaggagca cgtgcccacc tcctcagcct cagctgggac ccctgtggag
     4981 gtgggcgaca gagacttcac cctggagccc ctgagagggg accagccccg ctcagaggtc
     5041 tgcggggggg acctggccct gcccccagtg cctaaggagg cggtctcagg gcgtgtccag
     5101 ctgccccagc ccttggtgga gaagtcagaa ctggccccca ctcgaggggc cgtgatggag
     5161 cagggcacgt cctcgtcaat gacagcagag tcgtctccca ggagtatgct aggctatgac
     5221 agagatggaa ggcaggtggc ctcagactcc catgtggtcc ccagcgtccc ccaggatgta
     5281 cctgcttttg tgagacctgc acgtgtggag cccacagaca gggatggtgg agaagctgga
     5341 gaaagctcgg cagagccccc gccgagtgac atgggcacag tggggggcca ggctagccac
     5401 ccccagacac tcggcgctcg agctttgggg tcccctcgga aacgtccaga gcagcaggat
     5461 gtcagctcac cagccaagac tgtgggccgt ttctcggtgg tcagcactca ggacgagtgg
     5521 accctggcct ccccccacag cctgagatac tctgccccac ccgacgtcta cctggacgag
     5581 gccccctcca gccccgacgt gaagctggca gtgcggcggg cgcagacggc ctcctccatc
     5641 gaggtcggcg tgggcgagcc cgtgtccagc gactctgggg acgagggccc tcgggcgaga
     5701 cccccggtgc agaagcaggc gtccctgccc gtgagtggca gcgtggctgg cgacttcgtg
     5761 aagaaggcca ccgccttcct gcagaggcct tctcgggccg gctcgctggg ccccgagaca
     5821 cccagcaggg tgggcatgaa ggtccccacg atcagcgtga cctccttcca ttcccagtcg
     5881 tcctacatca gcagcgacaa tgattcggag ctcgaggatg ctgacataaa gaaggagctg
     5941 cagagtctgc gggagaagca cctgaaggag atctcggagc tgcagagcca gcagaagcag
     6001 gagatcgaag ctctgtaccg ccgcctgggc aagccactgc cccccaacgt gggcttcttc
     6061 cacacggcac cccccactgg ccgccggaga aaaaccagca agagcaagct gaaggcaggc
     6121 aagctgctaa atcccctggt gcggcagctc aaggtcgtgg cctccagcac aggtcacttg
     6181 gctgactcca gcagaggccc tcccgctaag gaccctgccc aagccagtgt ggggctcact
     6241 gcagacagca cgggcctgag cgggaaggca gtgcagaccc agcagccctg ctccgtccgg
     6301 gcctccctgt cttcggacat ctgctccggc ttagccagtg atggaggcgg agcgcgtggc
     6361 caaggctgga cggtttacca cccaacgtct gagagagtga cctataagtc tagtagcaaa
     6421 cctcgtgctc gattcctcag tggacccgta tctgtgtcca tctggtctgc cctgaagcgt
     6481 ctctgcctag gcaaagaaca cagcagtagg tcctccacca gcagcctggc cccaggccct
     6541 gagccaggcc cccagcccgc cctgcacgtc caggcgcagg tgaacaacag caacaacaag
     6601 aagggtacct tcacggacga cctgcacaag ctggtggacg agtggacgag caagacggtg
     6661 ggggccgcgc agctgaagcc cacgctcaac cagctgaagc agacccagaa gctgcaagac
     6721 atggaggccc aggcaggctg ggctgcccct ggcgaggcgc gggctatgac cgcacctcga
     6781 gcaggagtgg ggatgccacg tctgccccca gcgcccggcc ctctgtccac cacggtcatt
     6841 cccggagccg ccccgaccct gtccgtgccc acaccagatc ctgagagtga gaagcctgac
     6901 tgaccccgcc tagacgccag gcccacttca cgccgtctaa gtggagaagt gacggaccct
     6961 cagggccagc tgctcctcct gtccagttca cgctgttttg taaccacttt ctaagcattt
     7021 tttattcaca attggaaaca caaatgtaat gcaagaataa aaaatatttt ggggcagaaa
//
